Abstract:
:Coxsackievirus B3 (CVB-3) is a plus-strand RNA virus that is believed to be the most common causal agent of viral myocarditis. Since no specific treatment for CVB-3 infections is available to date, we and others have recently started to develop RNA interference (RNAi) approaches to prevent virus propagation. Here we describe our strategy for the development of efficient small interfering RNAs (siRNAs) against viral genomes. Initially, fusion constructs of a reporter (green fluorescent protein) and viral subgenomic fragments were employed to select active siRNAs against the virus. Moreover, in an attempt to achieve sustained virus silencing and reduce the risk of generating escape mutants, only highly efficient siRNAs directed against regions of the viral genome that are unlikely to tolerate mutations were considered for virus inhibition. Two siRNAs directed against the 3D RNA-dependent RNA polymerase were found to inhibit virus propagation by 80-90%. The protective effect of the efficient siRNAs lasted for several days. Furthermore, we have first evidence that inhibition of the cellular coxsackievirus-adenovirus receptor (CAR) by RNAi also reduces the virus titre. Our strategy is likely to be applicable to other (RNA) viruses as well.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Werk D,Schubert S,Lindig V,Grunert HP,Zeichhardt H,Erdmann VA,Kurreck Jdoi
10.1515/BC.2005.100keywords:
subject
Has Abstractpub_date
2005-09-01 00:00:00pages
857-63issue
9eissn
1431-6730issn
1437-4315journal_volume
386pub_type
杂志文章abstract::This review addresses many of the chemical aspects of nitrosative stress mediated by N2O3. From a cellular perspective, N2O3 and the resulting reactive nitrogen oxide species target specific motifs such as thiols, lysine active sites, and zinc fingers and is dependant upon both the rates of production as well as consu...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2004.001
更新日期:2004-01-01 00:00:00
abstract::Haptoglobin (Hpt) is known to capture circulating free hemoglobin (Hb) and bind apolipoprotein (Apo) A-I or E. Here, we report that Hb can be tightly bound by most of Hpt molecules (TB-Hpt, 80%), whereas loosely bound by a minor part of them (LB-Hpt, 20%). LB-Hpt amount was significantly increased (over 60%) in patien...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2011.033
更新日期:2011-04-01 00:00:00
abstract::Characterization of protein damage during photosensitization of chlorin e6-treated cells was performed using the green fluorescent protein (GFP). The GFP-chromophore damage caused by singlet oxygen was studied in COS 7 kidney cells and E. coli bacteria following light irradiation. Electron spin resonance (ESR) reveale...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.153
更新日期:2000-12-01 00:00:00
abstract::Understanding the molecular mechanisms behind extreme temperature stability is of relevance for the protein folding problem and for designing proteins for industrial and medical applications. A powerful approach for understanding the structural basis of thermostability is the comparison of high resolution structures o...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
abstract::Cytokinins are essential plant hormones that regulate numerous physiological processes. Recently, a protein was identified in mung bean (Vigna radiata) and characterized as a cytokinin-specific binding protein (VrCSBP). Fluorescence correlation spectroscopy was used to investigate the interaction between VrCSBP and it...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2010.005
更新日期:2010-01-01 00:00:00
abstract::We have previously shown that compatible organic osmolytes, such as betaine, myo-inositol and taurine, are part of the stress response of normal human keratinocytes (NHKs) to ultraviolet B (UVB) radiation. In this regard, we tested human HaCaT keratinocytes as a surrogate cell line for NHK. HaCaT cells osmo-dependentl...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.140
更新日期:2007-12-01 00:00:00
abstract::Hypochlorous acid (HOCl) is a bactericidal compound formed by activated neutrophils during inflammation. Overproduction of HOCl causes damage to tissues at the site of neutrophil accumulation. The deleterious effects of excessive HOCl formation can be attenuated using antioxidants. Thiols and thioethers are known to b...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2002.073
更新日期:2002-03-01 00:00:00
abstract::The albumin gene family arose in a series of duplication events which gave rise to symmetry in its structure. The four genes are tandemly linked on human chromosome 4q in the order: 5'ALB-5'AFP-5'ALF-5'DBP-centromere, and their introns display a symmetrical and repetitive pattern that is shared by members of the gene ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.183
更新日期:1999-12-01 00:00:00
abstract::The periplasmic ligand-binding protein ChoX is part of the ABC transport system ChoVWX that imports choline as a nutrient into the soil bacterium Sinorhizobium meliloti. We have recently reported the crystal structures of ChoX in complex with its ligands choline and acetylcholine and the structure of a fully closed bu...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2009.113
更新日期:2009-11-01 00:00:00
abstract::Abstract Few genes are known to be involved in renal cell carcinoma (RCC) development and progression. The cell-specific transcription factor hepatocyte nuclear factor 4 alpha (HNF4 alpha) is down-regulated in RCC and we have shown that HNF4 alpha inhibits cell proliferation in the embryonic kidney cell line HEK293. T...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.011
更新日期:2008-02-01 00:00:00
abstract::Dendritic cells (DCs) are an important link between innate and adaptive immunity. DCs get activated in inflamed tissues where oxygen tension is usually low, which requires the transcription factor hypoxia inducible factor (HIF)-1 for cellular adaptation. To investigate whether the HIF-1 transcriptional complex plays a...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2012-0320
更新日期:2013-04-01 00:00:00
abstract::The multifunctional helper component proteinase (HC-Pro) of potyviruses contains an autoproteolytic function that, together with the protein 1 (P1) and NIa proteinase, processes the polyprotein into mature proteins. In this study, we analysed the autoproteolytic active domain of zucchini yellow mosaic virus (ZYMV) HC-...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2011.097
更新日期:2011-10-01 00:00:00
abstract::Induction of apoptosis by selective BH3-mimetics is currently investigated as a novel strategy for cancer treatment. Here, we report that selective BH3-mimetics induce apoptosis in a variety of hematological malignancies. Apoptosis is accompanied by severe mitochondrial toxicities upstream of caspase activation. Speci...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2018-0233
更新日期:2019-01-28 00:00:00
abstract::The ATP-binding cassette (ABC) transporter genes represent the largest family of transporters and these genes are abundant in the genome of all vertebrates. Through analysis of the genome sequence databases we have characterized the full complement of ABC genes from several mammals and other vertebrates. Multiple gene...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2011.006
更新日期:2011-01-01 00:00:00
abstract::The Sialyl-Tn antigen (Sialyl alpha-Ser/Thr) is expressed as a cancer-associated antigen on the surface of cancer cells. Its presence is associated with a poor prognosis in patients with colorectal and other cancers. We previously reported that Sialyl-Tn expression in LSC human colon cancer cells could be explained by...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.029
更新日期:2001-02-01 00:00:00
abstract::Arginine deiminase (ADI) is a potential antitumor agent for the arginine deprivation treatment of L-arginine auxotrophic tumors. The optimum pH of ADI varies significantly, yet little is known about the origin of this variety. Here, Pseudomonas aeruginosa ADI (PaADI), an enzyme that functions only at acidic pH, was ut...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2012-0183
更新日期:2012-09-01 00:00:00
abstract::CYP20A1 is a well-conserved member of the human cytochrome P450 enzyme family for which no endogenous or xenobiotic substrate is known. We have recently shown that this enzyme has moderate activity towards two proluciferin probe substrates. In order to facilitate the search for physiological substrates we have tested ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2019-0307
更新日期:2020-02-25 00:00:00
abstract::Fluorescence correlation spectroscopy (FCS) was used in monitoring human parvovirus B19 virus-like particle (VLP) antibody complexes from acute phase and past-immunity serum samples. The Oregon Green 488-labeled VLPs gave an average diffusion coefficient of 1.7 x 10(-7) cm2 s(-1) with an apparent hydrodynamic radius o...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.011
更新日期:2004-01-01 00:00:00
abstract::We present a novel strategy, termed CISTEM, which allows direct in vivo screening of polypeptides displayed on the surface of E. coli cells by a combination of ligand-mediated protection and phage-mediated selection. The effectiveness of this new approach was demonstrated by displaying the T7.tag on the surface of E. ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.202
更新日期:2001-12-01 00:00:00
abstract::Two members of the nuclear receptor superfamily, EcR (ecdysteroid receptor protein) and Usp (Ultraspiracle), heterodimerize to form a functional receptor for the steroid hormone 20-hydroxyecdysone and thus enable it to coordinate morphogenetic events during insect metamorphosis. N-terminally His-tagged Usp was overexp...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.007
更新日期:2003-01-01 00:00:00
abstract::The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. Experiments using anti-peptide antisera indicated that this 556-aa protein is nuclear throughout the cell cycle and excluded from condensed chromatin during mitosis. Based on its electrophoretic migration and sensitivity to ph...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.045
更新日期:2007-04-01 00:00:00
abstract::Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes. ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.062
更新日期:2001-03-01 00:00:00
abstract::As the outermost barrier of the body, the skin is directly and frequently exposed to a prooxidative environment, including solar UVA and UVB radiation, and air pollution. Ozone, a major air pollutant, possesses a strong oxidizing potential and therefore is possibly one of the most reactive chemicals the skin ever meet...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/bchm.1997.378.11.1299
更新日期:1997-11-01 00:00:00
abstract::TNF-alpha is a major pro-inflammatory cytokine that regulates further cytokine induction, especially of IL-1 and IL-6, in many human diseases including cancer, inflammation and immune disorders. In a search for new inhibitors of inducible TNF-alpha promoter activity and expression, cultures of the imperfect fungus Tri...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.066
更新日期:2007-06-01 00:00:00
abstract::The hair follicle consists of a complex system of multiple tissue compartments that are clearly distinguishable by their morphology and type of differentiation. We have synthesized hair follicle-specific keratins from the companion layer (K6hf, K17) and the hair cortex (Ha1, Hb3, Hb6) in Escherichia coli. The assembly...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2002.156
更新日期:2002-09-01 00:00:00
abstract::The formation of reactive oxygen species (ROS) can be induced by xenobiotic substances, such as redox cycling molecules, but also by endogenous substances such as hormones and cytokines. Recent research shows the importance of ROS in cellular signaling. Here, the signaling pathways of the two blood pressure-regulating...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2010.136
更新日期:2010-11-01 00:00:00
abstract::The enzymatic properties of glycosylphosphatidylinositol-specific phospholipase D (EC 3.1.4.50) were characterized using a 6,000-fold purified enzyme. This was obtained in 100 microg amounts from human serum with a recovery of 35%. Pure alkaline phosphatase containing one anchor moiety per molecule was used as substra...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.062
更新日期:2000-05-01 00:00:00
abstract::Most excitatory synaptic transmissions in the central nervous system are mediated by the neurotransmitter glutamate. Binding of glutamate released from the presynaptic membrane causes glutamate receptors in the postsynaptic membrane to open, which results in a transient depolarization of the postsynaptic membrane. The...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.024
更新日期:2006-02-01 00:00:00
abstract::The possible application of proteinase inhibitors in the support of anti-tumor chemotherapy requires profound knowledge of the proteinases involved in malignant processes. Therefore, the occurrence of cathepsins B, D, H, L and S and of gelatinases, urokinase plasminogen activator and stromelysins was studied in biopsi...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm3.1996.377.11.695
更新日期:1996-11-01 00:00:00
abstract::The transcription of the fatty acid synthase (FAS) gene is regulated by the sterol status of the cell via cleavage of the sterol regulatory element-binding protein (SREBP). When human HepG2 hepatoma cells were cotransfected with an expression plasmid for mature SREBP-1a together with FAS promoter/reporter constructs s...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.136
更新日期:2001-07-01 00:00:00