Inhibition of cathepsin L-like proteases by cathepsin V propeptide.

abstract：:Abstract The R- and K-gingipain proteases of Porphyromonas gingivalis are involved in proteolysis of haemoglobin from which the defensive dimeric haem pigment is formed. Whilst oxyhaemoglobin is refractory towards K-gingipain, methaemoglobin is rapidly degraded. Ligation of methaemoglobin with N3-, which effectively b...

journal_title：Biological chemistry

authors： Smalley JW,Birss AJ,Szmigielski B,Potempa J

更新日期：2008-09-01 00:00:00

abstract：:RNAs are key players in life as they connect the genetic code (DNA) with all cellular processes dominated by proteins. They contain a variety of chemical modifications and many RNAs fold into complex structures. Here, we review recent progress in the analysis of RNA modification and structure on the basis of stable is...

journal_title：Biological chemistry

authors： Asadi-Atoi P,Barraud P,Tisne C,Kellner S

更新日期：2019-06-26 00:00:00

abstract：:Acid ceramidase (N-acylsphingosine deacylase, EC 3.5.1.23; AC) is the lipid hydrolase responsible for the degradation of ceramide into sphingosine and free fatty acids within lysosomes. The enzymatic activity was first identified over four decades ago and is deficient in two rare inherited disorders, Farber lipogranul...

journal_title：Biological chemistry

authors： Frohbergh M,He X,Schuchman EH

更新日期：2015-06-01 00:00:00

abstract：:Human inositol phosphate multikinase (IPMK) is a multifunctional protein in cellular signal transduction, namely, a multispecific inositol phosphate kinase, phosphatidylinositol 3-kinase, and a scaffold within the mTOR-raptor complex. To fulfill these nuclear and cytoplasmic functions, intracellular targeting of IPMK ...

journal_title：Biological chemistry

authors： Meyer R,Nalaskowski MM,Ehm P,Schröder C,Naj X,Brehm MA,Mayr GW

更新日期：2012-03-01 00:00:00

abstract：:As a crucial protein, the herpes simplex virus 1 (HSV-1) UL2 protein has been shown to take part in various stages of viral infection, nonetheless, its exact subcellular localization and transport molecular determinants are not well known thus far. In the present study, by using live cells fluorescent microscopy assay...

journal_title：Biological chemistry

authors： Cai M,Huang Z,Liao Z,Chen T,Wang P,Jiang S,Chen D,Peng T,Bian Y,Hong G,Yang H,Zeng Z,Li X,Li M

更新日期：2017-04-01 00:00:00

abstract：:TNF-alpha is a major pro-inflammatory cytokine that regulates further cytokine induction, especially of IL-1 and IL-6, in many human diseases including cancer, inflammation and immune disorders. In a search for new inhibitors of inducible TNF-alpha promoter activity and expression, cultures of the imperfect fungus Tri...

journal_title：Biological chemistry

authors： Rether J,Serwe A,Anke T,Erkel G

更新日期：2007-06-01 00:00:00

abstract：:Historically considered as accessory cells to neurons, there is an increasing interest in the role of astrocytes in normal and pathological conditions. Astrocytes are involved in neurotransmitter recycling, antioxidant supply, ion buffering and neuroinflammation, i.e. a lot of the same pathways that go astray in Alzhe...

journal_title：Biological chemistry

authors： Zulfiqar S,Garg P,Nieweg K

更新日期：2019-08-27 00:00:00

abstract：:There is increasing evidence that apoptosis and necrosis represent only two of several possible ways for cells to die. These two types of demise can occur simultaneously in tissues or cell cultures exposed to the same stimulus, and often local metabolic conditions and the intensity of the same initial insult decide th...

journal_title：Biological chemistry

authors： Nicotera P,Leist M,Single B,Volbracht C

更新日期：1999-09-01 00:00:00

abstract：:Recent progress in the identification and partial characterization of novel genes encoding cysteine proteases of the papain family has considerably increased our knowledge of this family of enzymes. Kinetic data available to date for this large family indicate relatively broad, overlapping specificities for most enzym...

journal_title：Biological chemistry

authors： Nägler DK,Ménard R

更新日期：2003-06-01 00:00:00

abstract：:Abstract The homeostasis and atheroprotective function of bilirubin could be an appealing model to investigate one of the many physiologic functions of the human aryl hydrocarbon receptor (AhR). Several clinical and epidemiological studies have been carried out on key enzymes generating and eliminating bilirubin (heme...

journal_title：Biological chemistry

authors： Bock KW,Köhle C

更新日期：2010-06-01 00:00:00

abstract：:Cellular volume regulation is fundamental for numerous physiological processes. The volume-regulated anion channel, VRAC, plays a crucial role in regulatory volume decrease. This channel, which is ubiquitously expressed in vertebrates, has been vastly characterized by electrophysiological means. It opens upon cell swe...

journal_title：Biological chemistry

authors： Stauber T

更新日期：2015-09-01 00:00:00

abstract：:Vaccination with either exogenous hepatitis B surface antigen (HBsAg) lipoprotein particles without adjuvants, or plasmid DNA encoding secreted small HBsAg stimulate long-lasting, potent antibody responses in H-2d (BALB/c) and C57Bl/6 (H-2b) mice. Vaccination with exogenous HBsAg primes MHC-I restricted cytotoxic T ly...

journal_title：Biological chemistry

authors： Schirmbeck R,Reimann J

更新日期：1999-03-01 00:00:00

abstract：:In this review, we discuss how time-resolved Fourier transform infrared (FTIR) spectroscopy is used to understand how GTP hydrolysis is catalyzed by small GTPases and their cognate GTPase-activating proteins (GAPs). By interaction with small GTPases, GAPs regulate important signal transduction pathways and transport m...

journal_title：Biological chemistry

authors： Kötting C,Gerwert K

更新日期：2015-02-01 00:00:00

abstract：:Stromal cell-derived factor-1alpha (SDF-1alpha) is a strong migratory stimulant for hematopoietic stem and progenitor cells (HSPCs). The hematopoietic cytokines thrombopoietin (TPO), Flt3-ligand (FL), stem cell factor (SCF) and interleukin 11 (IL-11) are able to stimulate amplification of primitive murine hematopoieti...

journal_title：Biological chemistry

authors： Kassmer SH,Niggemann B,Punzel M,Mieck C,Zänker KS,Dittmar T

更新日期：2008-07-01 00:00:00

abstract：:Human kallikrein-related peptidases (KLKs), including the well-known prostate cancer biomarker, prostate-specific antigen (PSA-KLK3), along with 14 other serine proteinase KLK family members are now known to regulate cells by cleaving and activating members of the G-protein-coupled proteinase-activated receptor (PAR) ...

journal_title：Biological chemistry

authors： Hollenberg MD

更新日期：2014-09-01 00:00:00

abstract：:The regulation of eukaryotic gene expression is a complicated process involving the interaction of a large number of transacting factors with specific cis-regulatory elements. DNA methylation plays a role in this scheme by acting in cis to modulate protein-DNA interactions. Several lines of evidence indicate that meth...

journal_title：Biological chemistry

authors： Bergman Y,Mostoslavsky R

更新日期：1998-04-01 00:00:00

abstract：:Myocardial zinc dyshomeostasis is associated with caspase-3 activation, ErbB2 degradation and apoptosis during hypoxia/reoxygenation. Zinc pyrithione replenishes intracellular zinc, suppresses caspase-3, augments ErbB2 levels and improves cell survival. We hypothesize that zinc is capable of modulating redox and endop...

journal_title：Biological chemistry

authors： Bodiga VL,Vemuri PK,Nimmagadda G,Bodiga S

更新日期：2020-10-25 00:00:00

abstract：:Induction of apoptosis by selective BH3-mimetics is currently investigated as a novel strategy for cancer treatment. Here, we report that selective BH3-mimetics induce apoptosis in a variety of hematological malignancies. Apoptosis is accompanied by severe mitochondrial toxicities upstream of caspase activation. Speci...

journal_title：Biological chemistry

authors： Henz K,Al-Zebeeby A,Basoglu M,Fulda S,Cohen GM,Varadarajan S,Vogler M

更新日期：2019-01-28 00:00:00

abstract：:N-acyl homoserine lactones (AHLs) are quorum sensing (QS) signal molecules used by most Gram-negative pathogenic bacteria. In this article the lactonase activity of the preparations based on hexahistidine-tagged organophosphorus hydrolase (His6-OPH) towards AHLs was studied. Initially, three of the most interesting β-...

journal_title：Biological chemistry

authors： Aslanli A,Lyagin I,Efremenko E

更新日期：2018-07-26 00:00:00

abstract：:The three-dimensional structure of pterin-4a-carbinolamine dehydratase (PCD) from Pseudomonas aeruginosa has been solved. Based on this we have investigated the roles of putative active center residues through functional replacement by site-directed mutagenesis. Three histidines, His73, His74 and His91, appear to be i...

journal_title：Biological chemistry

authors： Köster S,Stier G,Kubasch N,Curtius HC,Ghisla S

更新日期：1998-12-01 00:00:00

abstract：:In order to study the role of N-glycans in the ER-associated degradation of unassembled immunoglobulin light (Ig L) chains, we introduced N-glycan acceptor sites into the variable domain of the murine Ig L chain kappaNS1, which is unfolded in unassembled molecules. We investigated the fate of kappaNS1 glycosylated at ...

journal_title：Biological chemistry

authors： Chillarón J,Adán C,Haas IG

更新日期：2000-12-01 00:00:00

abstract：:Matrix metalloproteinases (MMPs) are a family of zinc endopeptidases that have been implicated in various disease processes. Different classes of MMP inhibitors, including hydroxamic acids, phosphinic acids and thiols, have been previously described. Most of these mimic peptides and most likely bind in a similar way t...

journal_title：Biological chemistry

authors： Grams F,Brandstetter H,D'Alò S,Geppert D,Krell HW,Leinert H,Livi V,Menta E,Oliva A,Zimmermann G,Gram F,Brandstetter H,D'Alò S,Geppert D,Krell HW,Leinert H,Livi VMenta E,Oliva A,Zimmermann G

更新日期：2001-08-01 00:00:00

abstract：:Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy has emerged as an efficient tool to elucidate the structure and the conformational dynamics of proteins under conditions close to the native state. This review article summarizes the basics as well as the recent p...

journal_title：Biological chemistry

authors： Klare JP

更新日期：2013-10-01 00:00:00

abstract：:Dipeptidyl peptidase-IV has been demonstrated to play a role in cancer biology by many authors. Since then, additional proteins possessing similar enzymatic activity have been described and their role in cancerogenesis has been hypothesized. To assess the complexity of these 'Dipeptidyl peptidase-IV activity and/or st...

journal_title：Biological chemistry

authors： Sedo A,Busek P,Scholzová E,Malík R,Vlasicová K,Janácková S,Mares V

更新日期：2004-06-01 00:00:00

abstract：:Mitochondria contain elaborate machineries for the import of precursor proteins from the cytosol. The translocase of the outer mitochondrial membrane (TOM) performs the initial import of precursor proteins and transfers the precursors to downstream translocases, including the presequence translocase and the carrier tr...

journal_title：Biological chemistry

authors： Straub SP,Stiller SB,Wiedemann N,Pfanner N

更新日期：2016-11-01 00:00:00

abstract：:The p53 tumour suppressor gene is frequently mutated in human tumours and different tumour-derived mutations have varying effects on cells. The effect of a novel tumour-derived p53 mutation and two recently described mutations from South African breast cancer patients on the growth rate, colony formation, cell cycle a...

journal_title：Biological chemistry

authors： Donninger H,Binder A,Bohm L,Parker MI

更新日期：2008-01-01 00:00:00

abstract：:Small-angle scattering (SAS) of X-rays and neutrons reveals low-resolution structures of biological macromolecules in solution. With the recent experimental and methodological advances, SAS became a unique tool for characterising biological systems. The method covers an extremely broad range of molecule sizes (from a ...

journal_title：Biological chemistry

authors： Svergun DI

更新日期：2010-07-01 00:00:00

abstract：:Human adenocarcinomas overexpress a hypoglycosylated, tumor-associated form of the mucin-like glycoprotein MUC1 containing abnormal mono- and disaccharide antigens, such as Tn, sialyl-Tn, and TF, as well as stretches of unglycosylated protein backbone in the variable number of tandem repeats (VNTR) region. Both peptid...

journal_title：Biological chemistry

authors： Ryan SO,Vlad AM,Islam K,Gariépy J,Finn OJ

更新日期：2009-07-01 00:00:00

abstract：:The GAGA box of the rat serine protease inhibitor 2 (spi 2) genes not only acts as a basal promoter element, but also mediates transcriptional activation by growth hormone and interleukin-6. The GAGA box is separated from the TATA box by only 12 bp, and this close association is required for efficient transcription. H...

journal_title：Biological chemistry

authors： Leverrier S,Cinato E,Paul C,Derancourt J,Bemark M,Leanderson T,Legraverend C

更新日期：2000-11-01 00:00:00

abstract：:A deletion mutant (V314) of the calcineurin A subunit was constructed using site-directed mutagenesis. Its phosphatase activity and function were then characterized. The V314 deletion significantly altered the phosphatase activity, which was more than ten times higher than that of wild-type calcineurin, the calcineuri...

journal_title：Biological chemistry

authors： Yan L,Wei Q

更新日期：1999-11-01 00:00:00