Abstract:
:Vaccination with either exogenous hepatitis B surface antigen (HBsAg) lipoprotein particles without adjuvants, or plasmid DNA encoding secreted small HBsAg stimulate long-lasting, potent antibody responses in H-2d (BALB/c) and C57Bl/6 (H-2b) mice. Vaccination with exogenous HBsAg primes MHC-I restricted cytotoxic T lymphocyte (CTL) responses to HBsAg in H-2d but not H-2b mice, while DNA vaccination primes HBsAg-specific CTL responses in both mouse strains. We defined vaccination strategies that could elicit CTL responses to exogenous HBsAg in 'low responder' C57Bl/6 mice. We found that the bacterial plasmid DNA itself, synthetic oligodeoxynucleotides containing immunostimulating sequences, or recombinant Th1 cytokines (IL12, IFNgamma) efficiently support priming of CTL responses to exogenous HBsAg in 'low responder' H-2b mice, but have only minor effects on CTL priming in 'high responder' H-2d mice in the high dose range tested. These molecularly well defined adjuvants can thus efficiently support priming of anti-viral T cell responses under 'low responder' conditions.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Schirmbeck R,Reimann Jdoi
10.1515/BC.1999.039keywords:
subject
Has Abstractpub_date
1999-03-01 00:00:00pages
285-91issue
3eissn
1431-6730issn
1437-4315journal_volume
380pub_type
杂志文章,评审abstract::MHC encoded DM heterodimers and classical MHC class II complexes meet in an endosomal/lysosomal compartment where DM heterodimers support peptide loading of MHC class II. Studies on peptide loading of rat class II and on peptide persistence in cells of the dendritic lineage prompted us to establish full length cDNA cl...
journal_title:Biological chemistry
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journal_title:Biological chemistry
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journal_title:Biological chemistry
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pub_type: 杂志文章,评审
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更新日期:2001-01-01 00:00:00
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更新日期:2000-11-01 00:00:00
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更新日期:2005-03-01 00:00:00
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