six-banded, a novel Drosophila gene, is expressed in 6 segmental stripes during embryonic development and in the eye imaginal disc.

abstract：:The cell, as a molecular system, is often interpreted in terms of complex clockworks, and the design charts of mechanical and electrical engineering are assumed to provide adequate approximations for the description of cellular organization. However, a growing body of experimental evidence obtained through the observa...

journal_title：Biological chemistry

authors： Kurakin A

更新日期：2005-03-01 00:00:00

abstract：:Bacterial proteases secreted into an infected host may exhibit a wide range of pathogenic potentials. Staphylococci, in particular Staphylococcus aureus, are known to produce several extracellular proteases, including serine-, cysteine- and metalloenzymes. Their insensitivity to most human plasma protease inhibitors a...

journal_title：Biological chemistry

authors： Dubin G

更新日期：2002-07-01 00:00:00

abstract：:The mitochondrial DNA (mtDNA) encompasses two classes of functionally important sequence variants: recent pathogenic mutations and ancient adaptive polymorphisms. To rapidly and cheaply evaluate both classes of single nucleotide variants (SNVs), we have developed an integrated system in which mtDNA SNVs are analyzed b...

journal_title：Biological chemistry

authors： Poole JC,Procaccio V,Brandon MC,Merrick G,Wallace DC

更新日期：2010-10-01 00:00:00

abstract：:The transcription of the fatty acid synthase (FAS) gene is regulated by the sterol status of the cell via cleavage of the sterol regulatory element-binding protein (SREBP). When human HepG2 hepatoma cells were cotransfected with an expression plasmid for mature SREBP-1a together with FAS promoter/reporter constructs s...

journal_title：Biological chemistry

authors： Wolf SS,Roder K,Sickinger S,Schweizer M

更新日期：2001-07-01 00:00:00

abstract：:Protein cofactors often are the business ends of proteins, and are either synthesized inside cells or are taken up from the nutrition. A cofactor that strictly needs to be synthesized by cells is the iron-sulfur (Fe/S) cluster. This evolutionary ancient compound performs numerous biochemical functions including electr...

journal_title：Biological chemistry

authors： Lill R

更新日期：2020-05-26 00:00:00

abstract：:The 15 human kallikrein-related peptidases (KLKs) are clinically important biomarkers and therapeutic targets of interest in inflammation, cancer, and neurodegenerative disease. KLKs are secreted as inactive pro-forms (pro-KLKs) that are activated extracellularly by specific proteolytic release of their amino-terminal...

journal_title：Biological chemistry

authors： Yoon H,Blaber SI,Li W,Scarisbrick IA,Blaber M

更新日期：2013-01-01 00:00:00

abstract：:Extreme thermophiles produce unusually long polyamines, including the linear caldopentamine (Cdp) and the branched pentamine tetrakis(3-aminopropyl)-ammonium (Taa), with the latter containing a central quaternary ammonium moiety. Here we compare the interaction of these two pentamines with RNA by studying the heat den...

journal_title：Biological chemistry

authors： Hayrapetyan A,Grosjean H,Helm M

更新日期：2009-09-01 00:00:00

abstract：:Mutations of the p53 gene may alter the specific regulatory domains of the protein. We examined the conserved domains III, IV and V by SSCP using PCR primers covering exons 5, 6, 7 and 8 from hairy cell leukemia (HCL), polyps, colorectal and gastric carcinomas. A low rate of p53 mutations was detected in HCL and polyp...

journal_title：Biological chemistry

authors： Marcsek ZL,König EA

更新日期：1998-04-01 00:00:00

abstract：:Historically considered as accessory cells to neurons, there is an increasing interest in the role of astrocytes in normal and pathological conditions. Astrocytes are involved in neurotransmitter recycling, antioxidant supply, ion buffering and neuroinflammation, i.e. a lot of the same pathways that go astray in Alzhe...

journal_title：Biological chemistry

authors： Zulfiqar S,Garg P,Nieweg K

更新日期：2019-08-27 00:00:00

abstract：:Seven purified proteins may be combined to reconstitute regulated, promoter-dependent RNA polymerase II transcription: five general transcription factors, Mediator, and RNA polymerase II. The entire system has been conserved across species from yeast to humans. The structure of RNA polymerase II, consisting of 10 poly...

journal_title：Biological chemistry

authors： Kornberg RD

更新日期：2001-08-01 00:00:00

abstract：:Nanomolar concentrations of human amylin promote death of RINm5F cells in a time- and concentrationdependent manner. Morphological changes of chromatin integrity suggest that cells are predominantly undergoing apoptosis. Human amylin induces significant activation of caspase-3 and strong and sustained phosphorylation ...

journal_title：Biological chemistry

authors： Rumora L,Hadzija M,Barisić K,Maysinger D,Grubiić TZ

更新日期：2002-11-01 00:00:00

abstract：:To elucidate the structures and dynamics of membrane proteins, highly advanced biophysical methods have been developed that often require significant resources, both for sample preparation and experimental analyses. For very complex systems, such as membrane transporters, ion channels or G-protein coupled receptors (G...

journal_title：Biological chemistry

authors： Rose-Sperling D,Tran MA,Lauth LM,Goretzki B,Hellmich UA

更新日期：2019-09-25 00:00:00

abstract：:Plasma kallikrein plays a role in coagulation, fibrinolysis and inflammation. Cathepsins B and L participate in (patho)physiological processes such as peptide antigen processing, tissue remodeling events, protein turnover in cells, hormone processing and tumor invasion. The present work analyzes the processing of prek...

journal_title：Biological chemistry

authors： Barros NM,Puzer L,Tersariol IL,Oliva ML,Sampaio CA,Carmona AK,Motta G

更新日期：2004-11-01 00:00:00

abstract：:Coxsackievirus B3 (CVB-3) is a plus-strand RNA virus that is believed to be the most common causal agent of viral myocarditis. Since no specific treatment for CVB-3 infections is available to date, we and others have recently started to develop RNA interference (RNAi) approaches to prevent virus propagation. Here we d...

journal_title：Biological chemistry

authors： Werk D,Schubert S,Lindig V,Grunert HP,Zeichhardt H,Erdmann VA,Kurreck J

更新日期：2005-09-01 00:00:00

abstract：:FK506-binding proteins (FKBPs) belong to a distinct class of immunophilins that interact with immunosuppressants. They use their peptidyl-prolyl isomerase (PPIase) activity to catalyze the cis-trans conversion of prolyl bonds in proteins during protein-folding events. FKBPs also act as a unique group of chaperones. Th...

journal_title：Biological chemistry

authors： Orłowski M,Popławska K,Pieprzyk J,Szczygieł-Sommer A,Więch A,Zarębski M,Tarczewska A,Dobrucki J,Ożyhar A

更新日期：2018-04-25 00:00:00

abstract：:Only one transcript for KLK10 was identified by RT-PCR in lung tissue, whereas KLK11 expressed at least four alternative transcripts. Quantitative analysis of KLK10 and KLK11 expression levels was assessed by real-time PCR, in a cohort of 47 patients with non-small-cell lung cancer (NSCLC). Expression levels of these ...

journal_title：Biological chemistry

authors： Planque C,Aïnciburu M,Heuzé-Vourc'h N,Régina S,de Monte M,Courty Y

更新日期：2006-06-01 00:00:00

abstract：:Oxidative stress has for a long time been associated with cell death, especially classical necrosis, however, its role in other cell death pathways is less clear. Here, we evaluated in a comparative way, the effect of four different reactive oxygen species (ROS) scavengers, N-acetyl-L-cysteine (NAC), α-tocopherol and ...

journal_title：Biological chemistry

authors： Kavčič N,Pegan K,Vandenabeele P,Turk B

更新日期：2019-01-28 00:00:00

abstract：:The molecular architectures of the catalytic core of protein phosphatase 1 (PP1) and protein phosphatase 2B (PP2B) are similar, and both contain a beta12-beta13 loop that consists of non-conserved residues. A truncation mutant containing the PP2B catalytic domain has previously been constructed in our laboratory, and ...

journal_title：Biological chemistry

authors： Xie XJ,Xue CZ,Huang W,Yu DY,Wei Q

更新日期：2006-10-01 00:00:00

abstract：:Ammonia is a major player in the pathogenesis of hepatic encephalopathy (HE) and affects astrocyte function by triggering a self-amplifying cycle between osmotic and oxidative stress. We recently demonstrated that hypoosmotic astrocyte swelling rapidly stimulates nitric oxide (NO) production and increases intracellula...

journal_title：Biological chemistry

authors： Kruczek C,Görg B,Keitel V,Bidmon HJ,Schliess F,Häussinger D

更新日期：2011-12-01 00:00:00

abstract：:Here, we describe a novel member in the group of membrane-anchored chymotrypsin (S1)-like serine proteases, namely testis serine protease 1 (T-SP1), as it is principally expressed in testis tissue. The human T-SP1 gene encompasses 28.7 kb on the short arm of chromosome 8 and consists of seven exons. Rapid amplificatio...

journal_title：Biological chemistry

authors： Neth P,Profanter B,Geissler C,Nägler DK,Nerlich A,Sommerhoff CP,Jochum M

更新日期：2008-12-01 00:00:00

abstract：:RNAs are key players in life as they connect the genetic code (DNA) with all cellular processes dominated by proteins. They contain a variety of chemical modifications and many RNAs fold into complex structures. Here, we review recent progress in the analysis of RNA modification and structure on the basis of stable is...

journal_title：Biological chemistry

authors： Asadi-Atoi P,Barraud P,Tisne C,Kellner S

更新日期：2019-06-26 00:00:00

abstract：:The lysosomal cysteine proteases cathepsin B and L play important roles in tumor cell invasion. An imbalance between these cathepsins and their endogenous inhibitors, the cystatins, has been associated with development of the metastatic phenotype. Accordingly, many studies have indicated potential use of cystatins in ...

journal_title：Biological chemistry

authors： Gianotti A,Sommer CA,Carmona AK,Henrique-Silva F

更新日期：2008-04-01 00:00:00

abstract：:The occurrence of rhodanese-like proteins in the major evolutionary phyla, together with the observed abundance of these proteins also within the same genome, suggests that their function cannot be limited to cyanide scavenging. The aim of the present study was to investigate whether Azotobacter vinelandii RhdA, an en...

journal_title：Biological chemistry

authors： Cereda A,Forlani F,Iametti S,Bernhardt R,Ferranti P,Picariello G,Pagani S,Bonomi F

更新日期：2003-10-01 00:00:00

abstract：:The mechanism of interaction of the lipopolysaccharide (LPS)-binding protein, LBP, with differently composed symmetric and asymmetric planar lipid bilayers was investigated in electrical measurements (membrane current, potential, capacitance). From a change of the inner membrane potential difference, binding of LBP to...

journal_title：Biological chemistry

authors： Gutsmann T,Haberer N,Carroll SF,Seydel U,Wiese A

更新日期：2001-03-01 00:00:00

abstract：:In bacteria, UGA stop codons can be recoded to direct the incorporation of selenocysteine into proteins on the ribosome. Recoding requires a selenocysteine incorporation sequence (SECIS) downstream of the UGA codon, a specialized translation factor SelB, and the non-canonical Sec-tRNASec, which is formed from Ser-tRNA...

journal_title：Biological chemistry

authors： Fischer N,Paleskava A,Gromadski KB,Konevega AL,Wahl MC,Stark H,Rodnina MV

更新日期：2007-10-01 00:00:00

abstract：:Plastids originated from an endosymbiotic event between an early eukaryotic host cell and an ancestor of today's cyanobacteria. During the events by which the engulfed endosymbiont was transformed into a permanent organelle, many genes were transferred from the plastidal genome to the nucleus of the host cell. Protein...

journal_title：Biological chemistry

authors： Vothknecht UC,Soll J

更新日期：2000-09-01 00:00:00

abstract：:The surface of spherical, nonporous silica nanoparticles (SiO2-NPs) was modified with gadolinium (Gd) complexes, fluorophores, and cell-penetrating peptides to achieve multifunctionality on a single particle. The Gd surface concentrations were 9-16 μmol/g resulting in nanomaterials with high local longitudinal and tra...

journal_title：Biological chemistry

authors： Joshi R,Feldmann V,Koestner W,Detje C,Gottschalk S,Mayer HA,Sauer MG,Engelmann J

更新日期：2013-01-01 00:00:00

abstract：:Metachromatic leukodystrophy is a lysosomal disorder caused by the deficiency of arylsulfatase A (ASA). This leads to the storage of the sphingolipid 3-O-sulfogalactosylceramide (sulfatide) in various cell types, such as renal tubular cells. Examination of mannose 6-phosphate receptor (MPR300)-dependent endocytosis re...

journal_title：Biological chemistry

authors： Klein D,Yaghootfam A,Matzner U,Koch B,Braulke T,Gieselmann V

更新日期：2009-01-01 00:00:00

abstract：:In order to study the role of N-glycans in the ER-associated degradation of unassembled immunoglobulin light (Ig L) chains, we introduced N-glycan acceptor sites into the variable domain of the murine Ig L chain kappaNS1, which is unfolded in unassembled molecules. We investigated the fate of kappaNS1 glycosylated at ...

journal_title：Biological chemistry

authors： Chillarón J,Adán C,Haas IG

更新日期：2000-12-01 00:00:00

abstract：:Pseudomonas aeruginosa and Serratia marcescens can cause refractory keratitis resulting in corneal perforation and blindness. These bacteria produce various kinds of proteases. In addition to pseudomonal elastase (LasB) and alkaline protease, LasA protease and protease IV have recently been found to be more important ...

journal_title：Biological chemistry

authors： Matsumoto K

更新日期：2004-11-01 00:00:00