Abstract：
:The lysosomal cysteine proteases cathepsin B and L play important roles in tumor cell invasion. An imbalance between these cathepsins and their endogenous inhibitors, the cystatins, has been associated with development of the metastatic phenotype. Accordingly, many studies have indicated potential use of cystatins in therapeutic approaches. We report a novel cystatin from sugarcane (Saccharum officinarum), CaneCPI-4, with strong inhibitory activity against cathepsins B (K(i) = 0.83 nM) and L (K(i) = 0.021 nM). The invasive ability of MDA-MB-231 human breast cancer cells expressing CaneCPI-4 was only slightly decreased. In contrast, addition of low, non-toxic concentrations of recombinant His-tagged CaneCPI-4 significantly reduced invasion through a Matrigel matrix. Immunoblot analyses failed to detect the recombinant protein inside cells, indicating that the cystatin was not internalized by endocytosis, but exerted its anti-invasive effect mainly through inhibition of extracellular cathepsins. Our findings open the possibility of considering phytocystatins for anti-cancer strategies.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Gianotti A,Sommer CA,Carmona AK,Henrique-Silva Fdoi
10.1515/BC.2008.035subject
Has Abstractpub_date
2008-04-01 00:00:00pages
447-53issue
4eissn
1431-6730issn
1437-4315journal_volume
389pub_type
杂志文章abstract：:The flagellate Euglena gracilis contains as photoreceptor complex a heterotetrameric light-sensitive adenylyl cyclase (AC), consisting of the flavoproteins PACalpha and PACbeta. Previously, we demonstrated the functional expression of PACalpha and PACbeta in oocytes from Xenopus laevis and of PACalpha in different ani...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2009.132
更新日期：2009-11-01 00:00:00
abstract：:The expression of the cysteine protease cathepsin B is increased in early stages of human breast cancer.To assess the potential role of cathepsin B in premalignant progression of breast epithelial cells, we employed a 3D reconstituted basement membrane overlay culture model of MCF10A human breast epithelial cells and ...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/hsz-2012-0252
更新日期：2012-12-01 00:00:00
abstract：:We have cloned from a chicken intestinal cDNA library Cmdr1, the first avian P-glycoprotein. Cmdr1 is 67% and 69% identical to proteins encoded by the human MDR1 and MDR2 genes, respectively. Functional expression of Cmdr1 in both mouse NIH 3T3 and yeast cells demonstrated that Cmdr1 represents the avian ortholog of h...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.1999.031
更新日期：1999-02-01 00:00:00
abstract：:Cysteine cathepsins are expressed in most tissues, including the gastrointestinal tract. We demonstrated an involvement of mouse intestinal cathepsin B in extracellular matrix remodeling for regeneration from trauma. The present study aimed at elucidating roles of cysteine cathepsins in the non-traumatized gastrointes...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/hsz-2014-0151
更新日期：2014-10-01 00:00:00
abstract：:An inert polysaccharide scaffold identified as a 5-15% component of prion rods (PrP 27-30) is unambiguously distinguishable from the N-glycosyl groups and the GPI anchor of PrP, and consists predominantly of 1,4-linked glucose with some branching via 1,4,6-linked glucose. We show that this polysaccharide scaffold is a...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2005.131
更新日期：2005-11-01 00:00:00
abstract：:Historically considered as accessory cells to neurons, there is an increasing interest in the role of astrocytes in normal and pathological conditions. Astrocytes are involved in neurotransmitter recycling, antioxidant supply, ion buffering and neuroinflammation, i.e. a lot of the same pathways that go astray in Alzhe...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/hsz-2019-0140
更新日期：2019-08-27 00:00:00
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journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/hsz-2015-0161
更新日期：2016-01-01 00:00:00
abstract：:Meprins are zinc-endopeptidases of the astacin family, which are expressed as membrane-bound or secreted forms in renal and intestinal brush-border membranes of mouse, rat and man. There are two types of meprin subunits, alpha and beta, which form disulfide-bonded homo- and heterodimers; further oligomerization is med...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2003.092
更新日期：2003-05-01 00:00:00
abstract：:Hsp70 chaperone systems play an essential role in the life cycle of many proteins not only in an hostile environment but also under normal growth conditions. In the course of evolution the diversification of functions was accompanied by an amplification of components of the Hsp70 system. Here strategies are reviewed h...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：
更新日期：1998-03-01 00:00:00
abstract：:The signal recognition particle (SRP), a ubiquitous cytoplasmic ribonucleoprotein particle, plays an essential role in promoting co-translational translocation of proteins into the endoplasmic reticulum. Here, we summarise recent progress made in the understanding of two essential SRP functions: the signal recognition...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/BC.1999.021
更新日期：1999-02-01 00:00:00
abstract：:Five moeA mutants were generated by replacing some conserved amino acids of MoeA by site-directed mutagenesis. The mutants were assayed for the ability to restore in vivo nitrate reductase activity of the moeA mutant Escherichia coli JRG97 and in vitro Neurospora crassa nit-1 nitrate reductase activity. The replacemen...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2002.034
更新日期：2002-02-01 00:00:00
abstract：:While the role of C2-ceramide in the induction of programmed cell death (PCD) in animal systems has been well documented, little is known of its role in plant cells. Here we show that C2-ceramide induces PCD in Arabidopsis suspension cultures, which is preceded by the generation of a calcium transient and an increase ...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2005.020
更新日期：2005-02-01 00:00:00
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journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/hsz-2015-0298
更新日期：2016-06-01 00:00:00
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journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2011.071
更新日期：2011-07-01 00:00:00
abstract：:G-protein coupled receptor 119 (GPR119) is emerging as a potential target for the treatment of type 2 diabetes with beneficial effects on glucose homeostasis. This study assessed the insulin-secreting properties of various GPR119 agonists and the distribution of GPR119 in pancreatic islets. Endogenous ligands [oleoyle...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/hsz-2013-0255
更新日期：2014-04-01 00:00:00
abstract：:Human septins comprise a family of 13 genes that encode for >30 protein isoforms with ubiquitous and tissue-specific expressions. Septins are GTP-binding proteins that assemble into higher-order oligomers and filamentous polymers, which associate with cell membranes and the cytoskeleton. In the last decade, much progr...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/hsz-2013-0233
更新日期：2014-02-01 00:00:00
abstract：:L-Arginine:glycine amidinotransferase (AT) catalyzes the committed step in creatine biosynthesis by formation of guanidinoacetic acid, the direct precursor of creatine. The X-ray structure of the human enzyme shows a novel fold with fivefold pseudosymmetry of beta beta alphabeta-modules. These modules enclose the acti...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：
更新日期：1997-03-01 00:00:00
abstract：:Abstract Fanconi anemia (FA) is a rare cancer-prone genetic disorder characterized by progressive bone marrow failure, chromosomal instability and redox abnormalities. There is much biochemical and genetic data, which strongly suggest that FA cells experience increased oxidative stress. The present study was designed ...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2011.064
更新日期：2011-07-01 00:00:00
abstract：:The non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase catalyses the irreversible reaction of glyceraldehyde-3-phosphate to 3-phosphoglycerate by the reduction of NADP to NADPH. This is in contrast to the extensively analysed phosphorylating glyceraldehyde-3-phosphate dehydrogenases which catalyse the revers...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：
更新日期：1997-12-01 00:00:00
abstract：:A FAD and [4Fe-4S]cluster-containing enzyme from Clostridium aminobutyricum catalyses the reversible dehydration of 4-hydroxybutyryl-CoA to crotonyl-CoA which involves the cleavage of an unactivated C-H bond at the beta-carbon. Transient oxidation of the substrate to an enoxy radical by FAD might facilitate the remova...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/bchm.1997.378.8.843
更新日期：1997-08-01 00:00:00
abstract：:Proteomic identification of protease cleavage site specificity (PICS) is a recent proteomic approach for the easy mapping of protease subsite preferences that determines both the prime- and non-prime side specificity concurrently. Here we greatly facilitate user access by providing an automated and simple web-based da...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2011.158
更新日期：2011-11-01 00:00:00
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journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2010.046
更新日期：2010-04-01 00:00:00
abstract：:The hair follicle consists of a complex system of multiple tissue compartments that are clearly distinguishable by their morphology and type of differentiation. We have synthesized hair follicle-specific keratins from the companion layer (K6hf, K17) and the hair cortex (Ha1, Hb3, Hb6) in Escherichia coli. The assembly...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2002.156
更新日期：2002-09-01 00:00:00
abstract：:Abstract The homeostasis and atheroprotective function of bilirubin could be an appealing model to investigate one of the many physiologic functions of the human aryl hydrocarbon receptor (AhR). Several clinical and epidemiological studies have been carried out on key enzymes generating and eliminating bilirubin (heme...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/BC.2010.065
更新日期：2010-06-01 00:00:00
abstract：:The three-dimensional structure of pterin-4a-carbinolamine dehydratase (PCD) from Pseudomonas aeruginosa has been solved. Based on this we have investigated the roles of putative active center residues through functional replacement by site-directed mutagenesis. Three histidines, His73, His74 and His91, appear to be i...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/bchm.1998.379.12.1427
更新日期：1998-12-01 00:00:00
abstract：:Five phage displayed peptide libraries were screened for binders to C1q, the recognition subunit of the classical complement pathway. Two rounds of panning resulted in the isolation and characterisation of several different phage displayed C1q-binding peptides from all five libraries. Two groups of the characterised p...
journal_title：Biological chemistry
pub_type： 评论,杂志文章
doi：10.1515/bchm.1997.378.12.1509
更新日期：1997-12-01 00:00:00
abstract：:The activities of cathepsin B, L, J and H in rat liver were significantly increased by starvation if compared with normal diet rats. Furthermore, the activity of cathepsin L increased with glucagon treatment, and the activities of cathepsin L and H decreased significantly with insulin treatment. The changes in catheps...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:In mammalian cells, glucosylceramide (GlcCer), the simplest glycosphingolipid, is hydrolyzed by the lysosomal enzyme acid beta-glucosidase (GlcCerase). In the human metabolic disorder Gaucher disease, GlcCerase activity is significantly decreased owing to one of approximately 200 mutations in the GlcCerase gene. The m...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/BC.2008.163
更新日期：2008-11-01 00:00:00
abstract：:Metachromatic leukodystrophy is a lysosomal disorder caused by the deficiency of arylsulfatase A (ASA). This leads to the storage of the sphingolipid 3-O-sulfogalactosylceramide (sulfatide) in various cell types, such as renal tubular cells. Examination of mannose 6-phosphate receptor (MPR300)-dependent endocytosis re...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2009.009
更新日期：2009-01-01 00:00:00
abstract：:A controlled interaction of materials with their surrounding biological environment is of great interest in many fields. Multifunctional coatings aim to provide simultaneous modulation of several biological signals. They can consist of various combinations of bioactive, and bioinert components as well as of reporter m...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/hsz-2016-0204
更新日期：2017-01-01 00:00:00
