Abstract:
:A controlled interaction of materials with their surrounding biological environment is of great interest in many fields. Multifunctional coatings aim to provide simultaneous modulation of several biological signals. They can consist of various combinations of bioactive, and bioinert components as well as of reporter molecules to improve cell-material contacts, prevent infections or to analyze biochemical events on the surface. However, specific immobilization and particular assembly of various active molecules are challenging. Herein, an overview of multifunctional coatings for biomaterials is given, focusing on synthetic strategies and the biological benefits by displaying several motifs.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Pagel M,Beck-Sickinger AGdoi
10.1515/hsz-2016-0204subject
Has Abstractpub_date
2017-01-01 00:00:00pages
3-22issue
1eissn
1431-6730issn
1437-4315pii
/j/bchm.just-accepted/hsz-2016-0204/hsz-2016-0204.journal_volume
398pub_type
杂志文章,评审abstract::Knockout mice are the gold standard to probe for the role of a specific protease within the interacting network of proteases, substrates, and inhibitors. This proteolytic network, or protease web, determines cell signaling and organ homeostasis. Therefore, protease deficiency or inhibition is intrinsically tied to alt...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2009.015
更新日期:2009-02-01 00:00:00
abstract::Isolated human granulocyte plasma membranes contain progelatinase B. The binding of progelatinase B to the membrane, however, is relatively weak, and a considerable part of progelatinase B can be removed by simply washing the membrane with buffer. This detachment does not depend on the ionic strength of the buffer, in...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.007
更新日期:2000-01-01 00:00:00
abstract::The mechanism of interaction of the lipopolysaccharide (LPS)-binding protein, LBP, with differently composed symmetric and asymmetric planar lipid bilayers was investigated in electrical measurements (membrane current, potential, capacitance). From a change of the inner membrane potential difference, binding of LBP to...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.052
更新日期:2001-03-01 00:00:00
abstract::The BCL2 family of genes (B-cell CLL/lymphoma 2; Bcl-2) plays a pivotal role in the highly regulated process of apoptosis. We have recently cloned a newly identified member of this family, BCL2L12, which was found to be differentially expressed in many tumors. It is known that topotecan and methotrexate act through in...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.203
更新日期:2006-12-01 00:00:00
abstract::We have characterised a Drosophila P-element enhancer detector insertion F125, which is expressed in the embryonic head and CNS as well as in various third instar imaginal discs. In an attempt to identify the gene with the equivalent expression pattern, we have characterised an adjacent gene. It encodes two novel conc...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1997.378.10.1119
更新日期:1997-10-01 00:00:00
abstract::The fundamental cellular role and molecular interactions of annexins in vesicle trafficking and membrane remodeling remain to be further clarified in order to better understand and exploit their contributions to health and disease. We focused on distinctive features of atypical annexins from all domains of life using ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2016-0273
更新日期:2017-06-27 00:00:00
abstract::Interleukin-13 (IL-13) is a T-cell-derived pleiotropic cytokine of particular medical importance because of its critical role in the development of allergic asthma. The effects of IL-13 on its target cells are mediated through a dimeric transmembrane receptor (IL-13R), which shares the IL-4Ralpha subunit with the IL-4...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.036
更新日期:2007-03-01 00:00:00
abstract::Among the Ras family, Ran is a unique small G protein. It does not have a lipid modification motif at the C-terminus to bind to the membrane, which is often observed within the Ras family. Ran may therefore interact with a wide range of proteins in various intracellular locations. This means that Ran could play many d...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2000.052
更新日期:2000-05-01 00:00:00
abstract::We present a method that allows preparing long DNA containing defined mismatches without the use of gel electrophoretic or chromatographic purification steps. The preparation starts with the synthesis of two PCR products, which are identical except for those positions that will later form the mismatches. One of the PC...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2002.166
更新日期:2002-09-01 00:00:00
abstract::Understanding the molecular mechanisms behind extreme temperature stability is of relevance for the protein folding problem and for designing proteins for industrial and medical applications. A powerful approach for understanding the structural basis of thermostability is the comparison of high resolution structures o...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
abstract::Cellular volume regulation is fundamental for numerous physiological processes. The volume-regulated anion channel, VRAC, plays a crucial role in regulatory volume decrease. This channel, which is ubiquitously expressed in vertebrates, has been vastly characterized by electrophysiological means. It opens upon cell swe...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2015-0127
更新日期:2015-09-01 00:00:00
abstract::The average amount of cholesterol in the whole animal equals approximately 2100 mg/kg body weight, and 15% and 23% of this sterol in the mouse and human, respectively, is found in the central nervous system. There is no detectable uptake across the blood-brain barrier of cholesterol carried in lipoproteins in the plas...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2009.035
更新日期:2009-04-01 00:00:00
abstract::Proteomic identification of protease cleavage site specificity (PICS) is a recent proteomic approach for the easy mapping of protease subsite preferences that determines both the prime- and non-prime side specificity concurrently. Here we greatly facilitate user access by providing an automated and simple web-based da...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2011.158
更新日期:2011-11-01 00:00:00
abstract::Bromelain isoinhibitors from pineapple stem (BIs) are unique double-chain inhibitors and inhibit the cysteine proteinase bromelain competitively. The three-dimensional structure was shown to be composed of two distinct domains, each of which is formed by a three-stranded anti-parallel beta-sheet. Unexpectedly, BIs wer...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2002.126
更新日期:2002-07-01 00:00:00
abstract::92 kDa and 72 kDa gelatinases, two neutral proteinases exhibiting elastinolytic activity and secreted as zymogens by aortic smooth muscle cells, were shown to bind to insoluble elastin. The active form of each enzyme interacted with substrate more avidly than latent form. Once bound to insoluble elastin, 92 kDa progel...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1997.378.3-4.265
更新日期:1997-03-01 00:00:00
abstract::Bacterial infections often upregulate the plasma kinin-forming cascade of the host (the 'contact system') which is triggered by adsorption of high molecular mass kininogen (HK), coagulation factor XII (FXII) and prekallikrein (pHPK) on the host or pathogen cell surfaces. A possible activation of the contact system upo...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2009.145
更新日期:2010-01-01 00:00:00
abstract::Cysteine proteases of the malaria parasite Plasmodium falciparum, known as falcipains, are promising targets for antimalarial chemotherapy. We evaluated cultured parasites for the stage-specific expression of cysteine proteases and sensitivity to cysteine protease inhibitors. Protease activity and inhibitor sensitivit...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2002.089
更新日期:2002-05-01 00:00:00
abstract::Nitrogen dioxide (*NO2) is an oxidizing free radical which can initiate a variety of destructive pathways in living systems, and several diseases are suspected to be connected with both exogenously and endogenously formed *NO2. Peroxynitrite (ONOO-/ONOOH) is believed to be an important endogenous source of *NO2 radica...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2002.043
更新日期:2002-03-01 00:00:00
abstract::This is the first report describing the enhancement of xylanase activity by the neutral amino acid glycine. Xylanase activity is increased seven-fold at alkaline pH in the presence of glycine and its pH optimum is shifted from pH 7 to 8 without using any protein engineering techniques. Analysis of the steady-state kin...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.007
更新日期:2007-01-01 00:00:00
abstract::A high-resolution crystallographic structure determination of a protein-ligand complex is generally accepted as the 'gold standard' for structure-based drug design, yet the relationship between structure and affinity is neither obvious nor straightforward. Here we analyze the interactions of a series of serine protein...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2014-0158
更新日期:2014-07-01 00:00:00
abstract::Protein disulfide isomerase (PDI) is a protein of the endoplasmic reticulum (ER) that is essential for the unscrambling of nonnative disulfide bonds. Here, we have determined the importance of PDI to both spore germination and vegetative cell division. To vary the concentration of PDI in the ER, we used plasmids that ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1997.378.5.431
更新日期:1997-05-01 00:00:00
abstract::Trypsin and chymotrypsin have similar tertiary structures, although very different substrate specificities. Trypsin hydrolyzes peptides at Lys/Arg residues while chymotrypsin recognizes large hydrophobic residues. Recent work has shown that trypsin is not converted into a protease with chymotrypsin-like activity when ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1996-07-01 00:00:00
abstract::Small RNAs such as microRNAs (miRNAs), short interfering RNAs (siRNAs) or Piwi-interacting RNAs (piRNAs) are important regulators of gene expression in various organisms. Small RNAs bind to a member of the Argonaute protein family and are incorporated into larger structures that mediate diverse gene silencing events. ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2014-0116
更新日期:2014-06-01 00:00:00
abstract::The cell, as a molecular system, is often interpreted in terms of complex clockworks, and the design charts of mechanical and electrical engineering are assumed to provide adequate approximations for the description of cellular organization. However, a growing body of experimental evidence obtained through the observa...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2005.030
更新日期:2005-03-01 00:00:00
abstract::Eukaryotic organisms have evolved complex and robust cellular stress response pathways to ensure maintenance of proteostasis and survival during fluctuating environmental conditions. Highly conserved stress response pathways can be triggered and coordinated at the cell-autonomous and cell-nonautonomous level by proteo...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2019-0385
更新日期:2020-08-27 00:00:00
abstract::Adenoviral DNA was examined within capsids by dimethyl sulfate footprinting. Protein-DNA interactions were visualized through ligation-mediated PCR (LM-PCR). Signals for protein binding were found adjacent to both inverted terminal repeats (ITR). There were no indications of close protein binding at several other loci...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.169
更新日期:2001-09-01 00:00:00
abstract::Metarhizium anisopliae is an entomopathogenic fungus relevant in biotechnology with applications like malaria vector control. Studies of its virulence factors are therefore of great interest. Fungal ribotoxins are toxic ribonucleases with extraordinary efficiency against ribosomes and suggested as potential insecticid...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2016-0119
更新日期:2017-01-01 00:00:00
abstract::Interleukin-6 (IL-6) is a 185 amino acid residue helical cytokine with various biological activities (e. g. B cell development, acute phase reaction). We have investigated the role of the 168-185 C-terminal region of IL-6 in the induction of fibrinogen synthesis and expression of junB mRNA using synthetic peptides cor...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.079
更新日期:2001-04-01 00:00:00
abstract::Components of kinin-forming systems operating at inflammatory sites are likely to interact with elastase that is released by recruited neutrophils and may, at least temporarily, constitute the major proteolytic activity present at these sites. The aim of this work was to determine the effect of kininogen degradation b...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.104
更新日期:2003-06-01 00:00:00
abstract::Eremophilanes are sesquiterpenes with a rearranged carbon skeleton formed both by plants and fungi, however, almost no plant eremophilanes are found in fungi. These eremophilanes possess mainly phytotoxic, antimicrobial, anticancer and immunomodulatory properties and in this review fungal eremophilanes with bioactivit...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2017-0171
更新日期:2017-12-20 00:00:00