Acid beta-glucosidase: insights from structural analysis and relevance to Gaucher disease therapy.

abstract：:The efficient execution of apoptotic cell death with the clearance of apoptotic debris by phagocytes is a key regulatory mechanism ensuring tissue homeostasis. Failure in this execution program contributes to the pathogenesis of many human diseases. In this review, we describe the current knowledge regarding the inter...

journal_title：Biological chemistry

authors： Weigert A,Jennewein C,Brüne B

更新日期：2009-05-01 00:00:00

abstract：:SlyD is a bacterial two-domain protein that functions as a molecular chaperone, a prolyl cis/trans isomerase, and a nickel-binding protein. This review summarizes recent findings about the molecular enzyme mechanism of SlyD. The chaperone function located in one domain of SlyD is involved in twin-arginine translocatio...

journal_title：Biological chemistry

authors： Kovermann M,Schmid FX,Balbach J

更新日期：2013-08-01 00:00:00

abstract：:Kallikrein-related peptidases (KLKs) are a family of serine proteases that were shown to be useful cancer biomarkers. KLKs have been shown to be dysregulated in prostate cancer (PCa). microRNAs (miRNAs) are short RNA nucleotides that negatively regulate gene expression and have been reportedly dysregulated in PCa. We ...

journal_title：Biological chemistry

authors： White NM,Youssef YM,Fendler A,Stephan C,Jung K,Yousef GM

更新日期：2012-04-01 00:00:00

abstract：:Protein transport, especially into different cellular compartments, is a highly coordinated and regulated process. The molecular machineries which carry out these transport processes are highly complex in structure, function, and regulation. In the case of chloroplasts, thousands of protein molecules have been estimat...

journal_title：Biological chemistry

authors： Sommer MS,Schleiff E

更新日期：2009-08-01 00:00:00

abstract：:Five phage displayed peptide libraries were screened for binders to C1q, the recognition subunit of the classical complement pathway. Two rounds of panning resulted in the isolation and characterisation of several different phage displayed C1q-binding peptides from all five libraries. Two groups of the characterised p...

journal_title：Biological chemistry

authors： Lauvrak V,Brekke OH,Ihle O,Lindqvist BH

更新日期：1997-12-01 00:00:00

abstract：:The N-terminal propeptide domains of several cathepsin L-like cysteine proteases have been shown to possess potent inhibitory activity. Here we report the first kinetic characterisation of the inhibition properties of the cathepsin V propeptide (CatV PP). Using a facile recombinant approach we demonstrate expression, ...

journal_title：Biological chemistry

authors： Burden RE,Snoddy P,Jefferies CA,Walker B,Scott CJ

更新日期：2007-05-01 00:00:00

abstract：:This article presents a historical account of the discovery of reversible protein phosphorylation. The process was uncovered in the mid-1950s in a study designed to elucidate the complex hormonal regulation of skeletal muscle glycogen phosphorylase. This enzyme was originally thought to be regulated by AMP now known t...

journal_title：Biological chemistry

authors： Fischer EH

更新日期：2010-02-01 00:00:00

abstract：:Few therapeutic options are offered to treat inflammation and alveolar wall destruction in emphysema. The effect of recombinant human pre-elafin, an elastase inhibitor, was evaluated in porcine pancreatic elastase (PPE)-induced emphysema in C57BL/6 mice. In a first protocol, mice received a single instillation of pre-...

journal_title：Biological chemistry

authors： Janelle MF,Doucet A,Bouchard D,Bourbonnais Y,Tremblay GM

更新日期：2006-07-01 00:00:00

abstract：:Vascular injury triggers endothelial exocytosis of granules, releasing pro-inflammatory and pro-thrombotic mediators into the blood. Nitric oxide (NO) and reactive oxygen species (ROS) limit vascular inflammation and thrombosis by inhibiting endothelial exocytosis. NO decreases exocytosis by regulating the activity of...

journal_title：Biological chemistry

authors： Lowenstein CJ,Tsuda H

更新日期：2006-10-01 00:00:00

abstract：:Latent episomal genomes of Epstein-Barr virus, a human gammaherpesvirus, represent a suitable model system for studying replication and methylation of chromosomal DNA in mammals. We analyzed the methylation patterns of CpG dinucleotides in the latent origin of DNA replication of Epstein-Barr virus using automated fluo...

journal_title：Biological chemistry

authors： Salamon D,Takacs M,Myöhänen S,Marcsek Z,Berencsi G,Minarovits J

更新日期：2000-02-01 00:00:00

abstract：:Small-angle scattering (SAS) of X-rays and neutrons reveals low-resolution structures of biological macromolecules in solution. With the recent experimental and methodological advances, SAS became a unique tool for characterising biological systems. The method covers an extremely broad range of molecule sizes (from a ...

journal_title：Biological chemistry

authors： Svergun DI

更新日期：2010-07-01 00:00:00

abstract：:Host T cell activation, a key step in obtaining adaptive immunity against pathogens, is initiated by the binding of the T cell receptor to a foreign antigenic peptide presented by the major histocompatibility complex on the surface of an antigen-presenting cell and, consequently, formation of an immunological synapse....

journal_title：Biological chemistry

authors： Beyersdorf N,Müller N

更新日期：2015-06-01 00:00:00

abstract：:The vast majority of malignancies detected in renal parenchyma are diagnosed as renal cell carcinoma (RCC), whose subtype discrimination and determination of prognosis may contribute to the selection of the adequate therapy. Recently, a new class of small non-coding RNAs, known as microRNAs, has proven to be among the...

journal_title：Biological chemistry

authors： Papadopoulos EI,Petraki C,Gregorakis A,Fragoulis EG,Scorilas A

更新日期：2016-06-01 00:00:00

abstract：:Annexins are a highly conserved protein family that bind to phospholipids in a calcium (Ca2+) - dependent manner. Studies with purified annexins, as well as overexpression and knockdown approaches identified multiple functions predominantly linked to their dynamic and reversible membrane binding behavior. However, mos...

journal_title：Biological chemistry

authors： Grewal T,Wason SJ,Enrich C,Rentero C

更新日期：2016-10-01 00:00:00

abstract：:The heterodimer consisting of ecdysteroid receptor (EcR) and ultraspiracle (USP), both of which are members of the nuclear receptor superfamily, is considered to be the functional ecdysteroid receptor. Here we analyzed the subcellular distribution of EcR and USP fused to fluorescent proteins. The experiments were carr...

journal_title：Biological chemistry

authors： Nieva C,Gwóźdź T,Dutko-Gwóźdź J,Wiedenmann J,Spindler-Barth M,Wieczorek E,Dobrucki J,Duś D,Henrich V,Ozyhar A,Spindler KD

更新日期：2005-05-01 00:00:00

abstract：:A kinetic study was performed of a model for an autocatalytic zymogen activation process involving both intra- and intermolecular routes, to which a chromogenic reaction in which the active enzyme acts upon one of its substrates was coupled to continuously monitor the reaction. Kinetic equations describing the evoluti...

journal_title：Biological chemistry

authors： Fuentes ME,Varón R,García-Moreno M,Valero E

更新日期：2005-07-01 00:00:00

abstract：:During the last years, the use of therapeutic glycoproteins has increased strikingly. Glycosylation of recombinant glycoproteins is of major importance in biotechnology, as the glycan composition of recombinant glycoproteins impacts their pharmacological properties. The terminal position of N-linked complex glycans in...

journal_title：Biological chemistry

authors： Werner A,Horstkorte R,Glanz D,Biskup K,Blanchard V,Berger M,Bork K

更新日期：2012-08-01 00:00:00

abstract：:Most excitatory synaptic transmissions in the central nervous system are mediated by the neurotransmitter glutamate. Binding of glutamate released from the presynaptic membrane causes glutamate receptors in the postsynaptic membrane to open, which results in a transient depolarization of the postsynaptic membrane. The...

journal_title：Biological chemistry

authors： Nakagawa T,Cheng Y,Sheng M,Walz T

更新日期：2006-02-01 00:00:00

abstract：:Human adenocarcinomas overexpress a hypoglycosylated, tumor-associated form of the mucin-like glycoprotein MUC1 containing abnormal mono- and disaccharide antigens, such as Tn, sialyl-Tn, and TF, as well as stretches of unglycosylated protein backbone in the variable number of tandem repeats (VNTR) region. Both peptid...

journal_title：Biological chemistry

authors： Ryan SO,Vlad AM,Islam K,Gariépy J,Finn OJ

更新日期：2009-07-01 00:00:00

abstract：:The signal recognition particle (SRP), a ubiquitous cytoplasmic ribonucleoprotein particle, plays an essential role in promoting co-translational translocation of proteins into the endoplasmic reticulum. Here, we summarise recent progress made in the understanding of two essential SRP functions: the signal recognition...

journal_title：Biological chemistry

authors： Bui N,Strub K

更新日期：1999-02-01 00:00:00

abstract：:Protein phosphatase 2A (PP2A) constitutes one of the major families of protein serine/threonine phosphatases found in all eukaryotic cells. PP2A holoenzymes are composed of a catalytic subunit complexed with a structural regulatory subunit of 65 kDa. These core subunits associate with regulatory subunits of various si...

journal_title：Biological chemistry

authors： Götz J,Kues W

更新日期：1999-09-01 00:00:00

abstract：:TNF-alpha is a major pro-inflammatory cytokine that regulates further cytokine induction, especially of IL-1 and IL-6, in many human diseases including cancer, inflammation and immune disorders. In a search for new inhibitors of inducible TNF-alpha promoter activity and expression, cultures of the imperfect fungus Tri...

journal_title：Biological chemistry

authors： Rether J,Serwe A,Anke T,Erkel G

更新日期：2007-06-01 00:00:00

abstract：:The bioactivation of inorganic nitrite refers to the conversion of otherwise 'inert' nitrite to the diatomic signaling molecule nitric oxide (NO), which plays important roles in human physiology and disease, notably in the regulation of vascular tone and blood flow. While the most well-known sources of NO are the nitr...

journal_title：Biological chemistry

authors： Amdahl MB,DeMartino AW,Gladwin MT

更新日期：2019-12-18 00:00:00

abstract：:Extreme thermophiles produce unusually long polyamines, including the linear caldopentamine (Cdp) and the branched pentamine tetrakis(3-aminopropyl)-ammonium (Taa), with the latter containing a central quaternary ammonium moiety. Here we compare the interaction of these two pentamines with RNA by studying the heat den...

journal_title：Biological chemistry

authors： Hayrapetyan A,Grosjean H,Helm M

更新日期：2009-09-01 00:00:00

abstract：:Thyroid hormones stimulate gap junctional communication in rat liver WB-F344 epithelial cells, elevating connexin43 mRNA and protein levels. In the present work we analysed connexin43 expression in liver and heart samples from thyroid hormone-treated Wistar rats. Connexin43 mRNA was elevated 2.1-fold in rat liver samp...

journal_title：Biological chemistry

authors： Stock A,Sies H

更新日期：2000-09-01 00:00:00

abstract：:Early diagnostics at the preclinical stage of Alzheimer's disease is of utmost importance for drug development in clinical trials and prognostic guidance. Since soluble Aβ oligomers are considered to play a crucial role in the disease pathogenesis, several methods aim to quantify Aβ oligomers in body fluids such as ce...

journal_title：Biological chemistry

authors： Kravchenko K,Kulawik A,Hülsemann M,Kühbach K,Zafiu C,Herrmann Y,Linnartz C,Peters L,Bujnicki T,Willbold J,Bannach O,Willbold D

更新日期：2017-04-01 00:00:00

abstract：:Knockout mice are the gold standard to probe for the role of a specific protease within the interacting network of proteases, substrates, and inhibitors. This proteolytic network, or protease web, determines cell signaling and organ homeostasis. Therefore, protease deficiency or inhibition is intrinsically tied to alt...

journal_title：Biological chemistry

authors： Krüger A

更新日期：2009-02-01 00:00:00

abstract：:FK506-binding proteins (FKBPs) belong to a distinct class of immunophilins that interact with immunosuppressants. They use their peptidyl-prolyl isomerase (PPIase) activity to catalyze the cis-trans conversion of prolyl bonds in proteins during protein-folding events. FKBPs also act as a unique group of chaperones. Th...

journal_title：Biological chemistry

authors： Orłowski M,Popławska K,Pieprzyk J,Szczygieł-Sommer A,Więch A,Zarębski M,Tarczewska A,Dobrucki J,Ożyhar A

更新日期：2018-04-25 00:00:00

abstract：:The kinin B2 receptor, which is constitutively expressed in a large number of tissues, mediates most of the known effects of bradykinin (BK). Normally undetectable in healthy tissues, the B1 receptor is strongly over-expressed under pathological conditions. BK is an important mediator in renal homeostasis and is mainl...

journal_title：Biological chemistry

authors： Bachvarov D,Bachvarova M,Koumangaye R,Klein J,Pesquero JB,Neau E,Bader M,Schanstra JP,Bascands JL

更新日期：2006-01-01 00:00:00

abstract：:The multifunctional helper component proteinase (HC-Pro) of potyviruses contains an autoproteolytic function that, together with the protein 1 (P1) and NIa proteinase, processes the polyprotein into mature proteins. In this study, we analysed the autoproteolytic active domain of zucchini yellow mosaic virus (ZYMV) HC-...

journal_title：Biological chemistry

authors： Boonrod K,Füllgrabe MW,Krczal G,Wassenegger M

更新日期：2011-10-01 00:00:00