Abstract:
:Knockout mice are the gold standard to probe for the role of a specific protease within the interacting network of proteases, substrates, and inhibitors. This proteolytic network, or protease web, determines cell signaling and organ homeostasis. Therefore, protease deficiency or inhibition is intrinsically tied to alterations within this network, always leading to new molecular phenotypes, which define susceptibility of an organ to disease. Furthermore, recent hints, mainly from research on matrix metalloproteinases, about the impact of the protease web on inter-organ signaling molecules suggest the existence of a proteolytic internet of communicating local organ- or molecular polymorphism-specific networks, thereby defining homeostasis and disease susceptibility in the whole organism.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Krüger Adoi
10.1515/BC.2009.015subject
Has Abstractpub_date
2009-02-01 00:00:00pages
91-7issue
2eissn
1431-6730issn
1437-4315pii
10.1515/BC.2009.015journal_volume
390pub_type
杂志文章,评审abstract::We present a method that allows preparing long DNA containing defined mismatches without the use of gel electrophoretic or chromatographic purification steps. The preparation starts with the synthesis of two PCR products, which are identical except for those positions that will later form the mismatches. One of the PC...
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