Abstract:
:Human adenocarcinomas overexpress a hypoglycosylated, tumor-associated form of the mucin-like glycoprotein MUC1 containing abnormal mono- and disaccharide antigens, such as Tn, sialyl-Tn, and TF, as well as stretches of unglycosylated protein backbone in the variable number of tandem repeats (VNTR) region. Both peptide and glycopeptide epitopes generated from the VNTR are candidates for cancer vaccines and we performed experiments to evaluate their relative potential to elicit tumor-MUC1-specific immunity. We show here that immunization with the 100 amino acid-long VNTR peptide (MUC1p) elicits weaker responses in MUC1 transgenic mice compared to wild type mice suggesting self-tolerance. In contrast, when glycosylated with tumor-associated Tn antigen (GalNAc-O-S/T), TnMUC1 induces glycopeptide-specific T cell and antibody responses in both strains of mice and helps enhance responses to MUC1p in MUC1 transgenic mice. Using newly derived MUC1-specific mouse T cell hybridomas we show that the only antigen-presenting cells able to cross-present TnMUC1 glycopeptide are dendritic cells (DCs). This is likely due to their exclusive expression of receptors capable of binding TnMUC1. We conclude that MUC1 glycopeptides induce stronger immunity in MUC1-Tg mice because they are recognized as 'foreign' rather than ;self' and because they are cross-presented preferentially by DCs.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Ryan SO,Vlad AM,Islam K,Gariépy J,Finn OJdoi
10.1515/BC.2009.070subject
Has Abstractpub_date
2009-07-01 00:00:00pages
611-8issue
7eissn
1431-6730issn
1437-4315journal_volume
390pub_type
杂志文章abstract::DNA-based immunization has been shown to induce protective immunity against several microbial pathogens including HIV-1. Several routes of DNA vaccination have been exploited. However, the properties of the immune responses seem to differ with the different routes used for DNA delivery, ultimately affecting the outcom...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.049
更新日期:1999-03-01 00:00:00
abstract::Here, we describe a novel member in the group of membrane-anchored chymotrypsin (S1)-like serine proteases, namely testis serine protease 1 (T-SP1), as it is principally expressed in testis tissue. The human T-SP1 gene encompasses 28.7 kb on the short arm of chromosome 8 and consists of seven exons. Rapid amplificatio...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.170
更新日期:2008-12-01 00:00:00
abstract::Based on studies presented here and other published experiments performed with surviving tissue preparations, with transfected cells and with cells that constitutively express the human angiotensin I converting enzyme ACE and B2 receptors, we concluded the following: ACE inhibitors and other endogenous peptides that r...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2001.007
更新日期:2001-01-01 00:00:00
abstract::Adenoviral DNA was examined within capsids by dimethyl sulfate footprinting. Protein-DNA interactions were visualized through ligation-mediated PCR (LM-PCR). Signals for protein binding were found adjacent to both inverted terminal repeats (ITR). There were no indications of close protein binding at several other loci...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.169
更新日期:2001-09-01 00:00:00
abstract::Magnesium cation (Mg2+) is the most abundant divalent cation in living cells, where it is required for various intracellular functions. In chloroplasts and cyanobacteria, established photosynthetic model systems, Mg2+ is the central ion in chlorophylls, and Mg2+ flux across the thylakoid membrane is required for count...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0476
更新日期:2019-09-25 00:00:00
abstract::MHC encoded DM heterodimers and classical MHC class II complexes meet in an endosomal/lysosomal compartment where DM heterodimers support peptide loading of MHC class II. Studies on peptide loading of rat class II and on peptide persistence in cells of the dendritic lineage prompted us to establish full length cDNA cl...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1997.378.9.1005
更新日期:1997-09-01 00:00:00
abstract::A large number of prostate cancer (PCa) patients receive treatment without significant benefits, strengthening the need for accurate prognosis, which can be supported by the study of miRNAs. In silico specificity analysis was performed for the identification of miRNAs able to regulate KLK2 and KLK4 expression. Total R...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2014-0150
更新日期:2014-09-01 00:00:00
abstract::Plastids originated from an endosymbiotic event between an early eukaryotic host cell and an ancestor of today's cyanobacteria. During the events by which the engulfed endosymbiont was transformed into a permanent organelle, many genes were transferred from the plastidal genome to the nucleus of the host cell. Protein...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2000.110
更新日期:2000-09-01 00:00:00
abstract::In a recent study we showed that two proteinases (CMS2MS2 and CMS2MS3) from Carica candamarcensis enhance mammalian cell proliferation. The aim of the present study is the determination of the primary structure of CMS2MS2 and prediction of its three-dimensional structure. The protein contains 214 residues, including t...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.083
更新日期:2007-08-01 00:00:00
abstract::The 15 members of the kallikrein-related serine peptidase (KLK) family have diverse tissue-specific expression profiles and roles in a range of cellular processes, including proliferation, migration, invasion, differentiation, inflammation and angiogenesis that are required in both normal physiology as well as patholo...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2014-0147
更新日期:2014-09-01 00:00:00
abstract::The cellular adhesion molecule E-selectin is expressed on activated endothelial cells, and is involved in the process of adherence of blood cells to vessel endothelium in inflammatory events such as atherosclerosis. In a recent study we found a Ser128Arg mutation in the EGF domain as well as a Leu554Phe mutation in th...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.082
更新日期:1999-06-01 00:00:00
abstract::The gene product of the yeast VPS1 gene is a member of a family of high-molecular-weight GTP-binding proteins that are involved in diverse cellular processes. The Vps1 protein (Vps1p) was shown to perform an essential function in the yeast secretory pathway. Here, we report the isolation and characterization of a muta...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:
更新日期:1997-10-01 00:00:00
abstract::Mammalian apolipoproteinB (apoB) RNA editing is a site-specific deamination reaction that mediates the C to U conversion responsible for apoB48 production in the mammalian small intestine. This process is not detected in chicken apoB RNA. Mammalian apoB RNA editing is mediated by a multicomponent enzyme complex that i...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1998.379.8-9.1075
更新日期:1998-08-01 00:00:00
abstract::The bifunctional protein DCoH (dimerization cofactor of HNF-1) is a transcriptional coactivator of homeodomain proteins and a cytoplasmic enzyme. Here we report on the isolation, nucleotide sequence and genomic structure of the chicken and rat DCoH genes. They have an unusual first exon coding for a single amino acid....
journal_title:Biological chemistry
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::HB13 human myoblasts express physiological and biochemical markers associated with myoblast differentiation in non-human cell culture model systems. During differentiation, HB13 myoblasts also demonstrate fusion-related increases in cathepsin B activity and protein levels. These increases are associated with an increa...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2002.207
更新日期:2002-12-01 00:00:00
abstract::Abstract Fanconi anemia (FA) is a rare cancer-prone genetic disorder characterized by progressive bone marrow failure, chromosomal instability and redox abnormalities. There is much biochemical and genetic data, which strongly suggest that FA cells experience increased oxidative stress. The present study was designed ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2011.064
更新日期:2011-07-01 00:00:00
abstract::Human kallikrein-related peptidases (KLKs), including the well-known prostate cancer biomarker, prostate-specific antigen (PSA-KLK3), along with 14 other serine proteinase KLK family members are now known to regulate cells by cleaving and activating members of the G-protein-coupled proteinase-activated receptor (PAR) ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2014-0123
更新日期:2014-09-01 00:00:00
abstract::Malaria can be a very severe disease, particularly in young children, pregnant women (mostly in primipara), and malaria naïve adults, and currently ranks among the most prevalent infections in tropical and subtropical areas throughout the world. The widespread occurrence and the increased incidence of malaria in many ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2001.069
更新日期:2001-04-01 00:00:00
abstract::The ATP-binding cassette (ABC) transporter genes represent the largest family of transporters and these genes are abundant in the genome of all vertebrates. Through analysis of the genome sequence databases we have characterized the full complement of ABC genes from several mammals and other vertebrates. Multiple gene...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2011.006
更新日期:2011-01-01 00:00:00
abstract::The enzyme L-asparaginase is a crucial component in the treatment of acute lymphoblastic leukemia (ALL). As all asparaginases in clinical use are derived from microorganisms, immunological reactions are the most important adverse events associated with asparaginase treatment. Two different methods, phage display and t...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.063
更新日期:2005-06-01 00:00:00
abstract::Interleukin-13 (IL-13) is a T-cell-derived pleiotropic cytokine of particular medical importance because of its critical role in the development of allergic asthma. The effects of IL-13 on its target cells are mediated through a dimeric transmembrane receptor (IL-13R), which shares the IL-4Ralpha subunit with the IL-4...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.036
更新日期:2007-03-01 00:00:00
abstract::Dendritic cells (DCs) are an important link between innate and adaptive immunity. DCs get activated in inflamed tissues where oxygen tension is usually low, which requires the transcription factor hypoxia inducible factor (HIF)-1 for cellular adaptation. To investigate whether the HIF-1 transcriptional complex plays a...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2012-0320
更新日期:2013-04-01 00:00:00
abstract::The neurobiological mechanisms of obesity-induced peripheral neuropathy are poorly understood. We evaluated the role of Sigma-1 receptor (Sig-1R) and NMDA receptor (NMDARs) in the spinal cord in peripheral neuropathy using an animal model of high fat diet-induced diabetes. We examined the expression of Sig-1R and NMDA...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2017-0117
更新日期:2017-09-26 00:00:00
abstract::Recent progress in the identification and partial characterization of novel genes encoding cysteine proteases of the papain family has considerably increased our knowledge of this family of enzymes. Kinetic data available to date for this large family indicate relatively broad, overlapping specificities for most enzym...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2003.094
更新日期:2003-06-01 00:00:00
abstract::Mitochondria contain elaborate machineries for the import of precursor proteins from the cytosol. The translocase of the outer mitochondrial membrane (TOM) performs the initial import of precursor proteins and transfers the precursors to downstream translocases, including the presequence translocase and the carrier tr...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2016-0145
更新日期:2016-11-01 00:00:00
abstract::Cells need to process multifaceted external cues to steer their dynamic behavior. To efficiently perform this task, cells implement several exploratory mechanisms to actively sample their environment. In particular, cells can use exploratory actin-based cell protrusions and contractions to engage and squeeze the envir...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2017-0341
更新日期:2018-07-26 00:00:00
abstract::Abstract Few genes are known to be involved in renal cell carcinoma (RCC) development and progression. The cell-specific transcription factor hepatocyte nuclear factor 4 alpha (HNF4 alpha) is down-regulated in RCC and we have shown that HNF4 alpha inhibits cell proliferation in the embryonic kidney cell line HEK293. T...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.011
更新日期:2008-02-01 00:00:00
abstract::Protein folding is one of the fundamental processes in life and therefore needs to be tightly regulated. Many cellular quality control systems are in place to ensure that proteostasis is optimally adjusted for a changing environment, facilitating protein folding, translocation and degradation. These systems include th...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0458
更新日期:2019-06-26 00:00:00
abstract::We have investigated the methylation status of the repeat unit of the human ribosomal RNA genes in lung cancer. Using a Southern blot analysis approach we have determined that the non-transcribed region of these genes was generally heavily methylated, while the transcribed region was not methylated in either tumor or ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.010
更新日期:1999-01-01 00:00:00
abstract::Oncogenic transformation is dependent on activated membrane-associated NADPH oxidase (NOX). However, the resultant extracellular superoxide anions are also driving the NO/peroxynitrite and the HOCl pathway, which eliminates NOX-expressing transformed cells through selective apoptosis induction. Tumor progression is de...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2014-0234
更新日期:2015-12-01 00:00:00