Abstract:
:In a recent study we showed that two proteinases (CMS2MS2 and CMS2MS3) from Carica candamarcensis enhance mammalian cell proliferation. The aim of the present study is the determination of the primary structure of CMS2MS2 and prediction of its three-dimensional structure. The protein contains 214 residues, including the catalytic triad composed of Cys(25), His(159), and Asn(175). A phylogenetic tree analysis demonstrated that CMS2MS2 ranks closer to chymopapain than to papain. The overall predicted three-dimensional structure is similar to proteinases from the papain family. These results suggest that minor structural differences within CMS2MS2 must account for its proliferative action.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Gomes MT,Bemquerer MP,Lopes MT,Richardson M,Júnior SO,Salas CEdoi
10.1515/BC.2007.083subject
Has Abstractpub_date
2007-08-01 00:00:00pages
819-22issue
8eissn
1431-6730issn
1437-4315journal_volume
388pub_type
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