Structural basis of [NiFe] hydrogenase maturation by Hyp proteins.

abstract：:As the outermost barrier of the body, the skin is directly and frequently exposed to a prooxidative environment, including solar UVA and UVB radiation, and air pollution. Ozone, a major air pollutant, possesses a strong oxidizing potential and therefore is possibly one of the most reactive chemicals the skin ever meet...

journal_title：Biological chemistry

authors： Thiele JJ,Podda M,Packer L

更新日期：1997-11-01 00:00:00

abstract：:Vaccination with either exogenous hepatitis B surface antigen (HBsAg) lipoprotein particles without adjuvants, or plasmid DNA encoding secreted small HBsAg stimulate long-lasting, potent antibody responses in H-2d (BALB/c) and C57Bl/6 (H-2b) mice. Vaccination with exogenous HBsAg primes MHC-I restricted cytotoxic T ly...

journal_title：Biological chemistry

authors： Schirmbeck R,Reimann J

更新日期：1999-03-01 00:00:00

abstract：:Magnesium cation (Mg2+) is the most abundant divalent cation in living cells, where it is required for various intracellular functions. In chloroplasts and cyanobacteria, established photosynthetic model systems, Mg2+ is the central ion in chlorophylls, and Mg2+ flux across the thylakoid membrane is required for count...

journal_title：Biological chemistry

authors： Pohland AC,Schneider D

更新日期：2019-09-25 00:00:00

abstract：:N.BstNBI is a unique restriction endonuclease isolated from Bacillus stearothermophilus. We have characterized the recognition sequence and the cleavage site of N.BstNBI. Mapping of cleavage sites of N.BstNBI showed that it recognizes an asymmetric sequence, 5' GAGTC 3', and cleaves only on the top strand 4 base pairs...

journal_title：Biological chemistry

authors： Morgan RD,Calvet C,Demeter M,Agra R,Kong H

更新日期：2000-11-01 00:00:00

abstract：:The main function of the epidermis is to establish a vital multifunctional barrier between the body and its external environment. A defective epidermal barrier is one of the key features of atopic dermatitis (AD), a chronic and relapsing inflammatory skin disorder that affects up to 20% of children and 2-3% of adults ...

journal_title：Biological chemistry

authors： Le Lamer M,Pellerin L,Reynier M,Cau L,Pendaries V,Leprince C,Méchin MC,Serre G,Paul C,Simon M

更新日期：2015-11-01 00:00:00

abstract：:The three isoforms of human Ins(1,4,5)P3 3-kinase (IP3K) show remarkable differences in their intracellular targeting. Whereas predominant targeting to the cytoskeleton and endoplasmic reticulum has been shown for IP3K-A and IP3K-B, rat IP3K-C shuttles actively between the nucleus and cytoplasm. In the present study w...

journal_title：Biological chemistry

authors： Nalaskowski MM,Windhorst S,Stockebrand MC,Mayr GW

更新日期：2006-05-01 00:00:00

abstract：:Nitrosative stress results from an increase in reactive nitrogen species (RNS) within the cell. Though the RNS - nitric oxide (·NO) and peroxynitrite (ONOO-) - play pivotal physiological roles, at elevated concentrations, these moieties can be poisonous to both prokaryotic and eukaryotic cells alike due to their capac...

journal_title：Biological chemistry

authors： Tharmalingam S,Alhasawi A,Appanna VP,Lemire J,Appanna VD

更新日期：2017-10-26 00:00:00

abstract：:The major enzymatic activity of the ribosome is the catalysis of peptide bond formation. The active site -- the peptidyl transferase center -- is composed of ribosomal RNA (rRNA), and interactions between rRNA and the reactants, peptidyl-tRNA and aminoacyl-tRNA, are crucial for the reaction to proceed rapidly and effi...

journal_title：Biological chemistry

authors： Beringer M,Rodnina MV

更新日期：2007-07-01 00:00:00

abstract：:The purpose of this work was to study the regulative role of the glutamate receptor found earlier in the brain mitochondria. In the present work a glutamate-dependent signaling system with similar features was detected in mitochondria of the heart. The glutamate-dependent signaling system in the heart mitochondria was...

journal_title：Biological chemistry

authors： Selin AA,Lobysheva NV,Nesterov SV,Skorobogatova YA,Byvshev IM,Pavlik LL,Mikheeva IB,Moshkov DA,Yaguzhinsky LS,Nartsissov YR

更新日期：2016-05-01 00:00:00

abstract：:Airway smooth muscle (ASM) controls airway narrowing and plays a pivotal role in the pathogenesis of asthma. MicroRNAs are small yet powerful gene tuners that regulate diverse cellular processes. Recent studies have demonstrated the versatile role of microRNAs in regulating multiple ASM phenotypes that are critically ...

journal_title：Biological chemistry

authors： Sun M,Lu Q

更新日期：2016-06-01 00:00:00

abstract：:We present a method that allows preparing long DNA containing defined mismatches without the use of gel electrophoretic or chromatographic purification steps. The preparation starts with the synthesis of two PCR products, which are identical except for those positions that will later form the mismatches. One of the PC...

journal_title：Biological chemistry

authors： Thomas E,Pingoud A,Friedhoff P

更新日期：2002-09-01 00:00:00

abstract：:A deletion mutant (V314) of the calcineurin A subunit was constructed using site-directed mutagenesis. Its phosphatase activity and function were then characterized. The V314 deletion significantly altered the phosphatase activity, which was more than ten times higher than that of wild-type calcineurin, the calcineuri...

journal_title：Biological chemistry

authors： Yan L,Wei Q

更新日期：1999-11-01 00:00:00

abstract：:Knockout mice are the gold standard to probe for the role of a specific protease within the interacting network of proteases, substrates, and inhibitors. This proteolytic network, or protease web, determines cell signaling and organ homeostasis. Therefore, protease deficiency or inhibition is intrinsically tied to alt...

journal_title：Biological chemistry

authors： Krüger A

更新日期：2009-02-01 00:00:00

abstract：:The ubiquitous calpains, mu- and m-calpain, have been implicated in essential physiological processes and various pathologies. Cell-permeable specific inhibitors are important tools to elucidate the roles of calpains in cultivated cells and animal models. The synthetic N-acetylated 27-mer peptide derived from exon B o...

journal_title：Biological chemistry

authors： Gil-Parrado S,Assfalg-Machleidt I,Fiorino F,Deluca D,Pfeiler D,Schaschke N,Moroder L,Machleidt W

更新日期：2003-03-01 00:00:00

abstract：:Studies of extracellular matrix (ECM) biology in the nervous system have mainly focused on laminin, fibronectin and tenascin-R, proteins that are present during nervous system development and normal function. However, during disease, fibrin, which physiologically is not present in the nervous tissue, is detected at ne...

journal_title：Biological chemistry

authors： Akassoglou K,Strickland S

更新日期：2002-01-01 00:00:00

abstract：:We present a rapid and simple method for the sexing of human and other primate DNA. Homologous introns from the X- and Y-linked zinc finger protein genes (ZFX and ZFY) are PCR amplified with one primer pair. An Alu element insertion in the ZFX intron results in a size difference in the amplicons which is resolved by a...

journal_title：Biological chemistry

authors： Wilson JF,Erlandsson R

更新日期：1998-10-01 00:00:00

abstract：:The lysosomal cysteine proteinase cathepsin B has been implicated in the progression of various human tumors including ovarian cancer. Included in this study were 63 patients with epithelial ovarian carcinoma. Follow-up information (median follow-up period 7 years) was available for all patients, among whom 42 (66.7%)...

journal_title：Biological chemistry

authors： Scorilas A,Fotiou S,Tsiambas E,Yotis J,Kotsiandri F,Sameni M,Sloane BF,Talieri M

更新日期：2002-07-01 00:00:00

abstract：:Among the Ras family, Ran is a unique small G protein. It does not have a lipid modification motif at the C-terminus to bind to the membrane, which is often observed within the Ras family. Ran may therefore interact with a wide range of proteins in various intracellular locations. This means that Ran could play many d...

journal_title：Biological chemistry

authors： Nishimoto T

更新日期：2000-05-01 00:00:00

abstract：:As an important protein, UL31 has been demonstrated to play multiple roles in herpes simplex virus 1 (HSV-1) replication. Previous studies showed that UL31 predominantly locates in the nucleus in chemical fixed cells and live cells, however, the determining mechanisms for its nuclear translocation is not clear. In the...

journal_title：Biological chemistry

authors： Cai M,Si J,Li X,Zeng Z,Li M

更新日期：2016-06-01 00:00:00

abstract：:In the SalI system, endonuclease activity can be only achieved in the presence of a functional modification gene. Thus, the DNA methyltransferase is involved in the control of restriction. By fusion of the restriction gene of the SalI system to the modification gene of the isospecific HgiDII system a hybrid type II re...

journal_title：Biological chemistry

authors： Pélaez AI,Ribas-Aparicio RM,Gómez A,Rodicio MR

更新日期：1998-04-01 00:00:00

abstract：:The mitochondrial DNA (mtDNA) encompasses two classes of functionally important sequence variants: recent pathogenic mutations and ancient adaptive polymorphisms. To rapidly and cheaply evaluate both classes of single nucleotide variants (SNVs), we have developed an integrated system in which mtDNA SNVs are analyzed b...

journal_title：Biological chemistry

authors： Poole JC,Procaccio V,Brandon MC,Merrick G,Wallace DC

更新日期：2010-10-01 00:00:00

abstract：:Synthetic biologists aim at engineering controllable biological parts such as DNA, RNA and proteins in order to steer biological activities using external inputs. Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-t...

journal_title：Biological chemistry

authors： Di Ventura B,Mootz HD

更新日期：2019-03-26 00:00:00

abstract：:During the last decade, an increasing number of new enzymes containing glycyl radicals in their active sites have been identified and biochemically characterised. These include benzylsuccinate synthase (Bss), 4-hydroxyphenylacetate decarboxylase (Hpd) and the coenzyme B12-independent glycerol dehydratase (Gdh). These ...

journal_title：Biological chemistry

authors： Selmer T,Pierik AJ,Heider J

更新日期：2005-10-01 00:00:00

abstract：:Mammalian apolipoproteinB (apoB) RNA editing is a site-specific deamination reaction that mediates the C to U conversion responsible for apoB48 production in the mammalian small intestine. This process is not detected in chicken apoB RNA. Mammalian apoB RNA editing is mediated by a multicomponent enzyme complex that i...

journal_title：Biological chemistry

authors： Anant S,Yu H,Davidson NO

更新日期：1998-08-01 00:00:00

abstract：:We have investigated the methylation status of the repeat unit of the human ribosomal RNA genes in lung cancer. Using a Southern blot analysis approach we have determined that the non-transcribed region of these genes was generally heavily methylated, while the transcribed region was not methylated in either tumor or ...

journal_title：Biological chemistry

authors： Shiraishi M,Sekiguchi A,Chuu YH,Sekiya T

更新日期：1999-01-01 00:00:00

abstract：:The cell, as a molecular system, is often interpreted in terms of complex clockworks, and the design charts of mechanical and electrical engineering are assumed to provide adequate approximations for the description of cellular organization. However, a growing body of experimental evidence obtained through the observa...

journal_title：Biological chemistry

authors： Kurakin A

更新日期：2005-03-01 00:00:00

abstract：:To elucidate the structures and dynamics of membrane proteins, highly advanced biophysical methods have been developed that often require significant resources, both for sample preparation and experimental analyses. For very complex systems, such as membrane transporters, ion channels or G-protein coupled receptors (G...

journal_title：Biological chemistry

authors： Rose-Sperling D,Tran MA,Lauth LM,Goretzki B,Hellmich UA

更新日期：2019-09-25 00:00:00

abstract：:The occurrence of rhodanese-like proteins in the major evolutionary phyla, together with the observed abundance of these proteins also within the same genome, suggests that their function cannot be limited to cyanide scavenging. The aim of the present study was to investigate whether Azotobacter vinelandii RhdA, an en...

journal_title：Biological chemistry

authors： Cereda A,Forlani F,Iametti S,Bernhardt R,Ferranti P,Picariello G,Pagani S,Bonomi F

更新日期：2003-10-01 00:00:00

abstract：:The cellular adhesion molecule E-selectin is expressed on activated endothelial cells, and is involved in the process of adherence of blood cells to vessel endothelium in inflammatory events such as atherosclerosis. In a recent study we found a Ser128Arg mutation in the EGF domain as well as a Leu554Phe mutation in th...

journal_title：Biological chemistry

authors： Wenzel K,Stahn R,Speer A,Denner K,Gläser C,Affeldt M,Moobed M,Scheer A,Baumann G,Felix SB

更新日期：1999-06-01 00:00:00

abstract：:Insulin contains three disulfide bonds, one intrachain bond, A6-A11, and two interchain bonds, A7-B7 and A20-B19. Site-directed mutagenesis results (the two cysteine residues of disulfide A7-B7 were replaced by serine) showed that disulfide A7-B7 is crucial to both the structure and activity of insulin. However, chemi...

journal_title：Biological chemistry

authors： Guo ZY,Jia XY,Feng YM

更新日期：2004-12-01 00:00:00