Abstract:
:Functional ribonuclease A was presented on the surface of the filamentous phage M13 by fusion to the minor coat protein. RNase activity of the fusion protein was shown by a zymogram assay. In addition, we established a modified method for preparing RNase-displaying phages without contaminating host RNases.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Korn K,Foerster HH,Hahn Udoi
10.1515/BC.2000.024keywords:
subject
Has Abstractpub_date
2000-02-01 00:00:00pages
179-81issue
2eissn
1431-6730issn
1437-4315journal_volume
381pub_type
杂志文章abstract::In order to enhance and target gene delivery we have previously established a novel method, termed magnetofection, which uses magnetic force acting on gene vectors that are associated with magnetic particles. Here we review the benefits, the mechanism and the potential of the method with regard to overcoming physical ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2003.082
更新日期:2003-05-01 00:00:00
abstract::Early diagnostics at the preclinical stage of Alzheimer's disease is of utmost importance for drug development in clinical trials and prognostic guidance. Since soluble Aβ oligomers are considered to play a crucial role in the disease pathogenesis, several methods aim to quantify Aβ oligomers in body fluids such as ce...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2016-0153
更新日期:2017-04-01 00:00:00
abstract::Abstract The R- and K-gingipain proteases of Porphyromonas gingivalis are involved in proteolysis of haemoglobin from which the defensive dimeric haem pigment is formed. Whilst oxyhaemoglobin is refractory towards K-gingipain, methaemoglobin is rapidly degraded. Ligation of methaemoglobin with N3-, which effectively b...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.140
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abstract::Mutations of the p53 gene may alter the specific regulatory domains of the protein. We examined the conserved domains III, IV and V by SSCP using PCR primers covering exons 5, 6, 7 and 8 from hairy cell leukemia (HCL), polyps, colorectal and gastric carcinomas. A low rate of p53 mutations was detected in HCL and polyp...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::Oxidative stress has for a long time been associated with cell death, especially classical necrosis, however, its role in other cell death pathways is less clear. Here, we evaluated in a comparative way, the effect of four different reactive oxygen species (ROS) scavengers, N-acetyl-L-cysteine (NAC), α-tocopherol and ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2017-0317
更新日期:2019-01-28 00:00:00
abstract::Metarhizium anisopliae is an entomopathogenic fungus relevant in biotechnology with applications like malaria vector control. Studies of its virulence factors are therefore of great interest. Fungal ribotoxins are toxic ribonucleases with extraordinary efficiency against ribosomes and suggested as potential insecticid...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2016-0119
更新日期:2017-01-01 00:00:00
abstract::We have investigated the methylation status of the repeat unit of the human ribosomal RNA genes in lung cancer. Using a Southern blot analysis approach we have determined that the non-transcribed region of these genes was generally heavily methylated, while the transcribed region was not methylated in either tumor or ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.010
更新日期:1999-01-01 00:00:00
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journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.131
更新日期:2005-11-01 00:00:00
abstract::Dog pancreas microsomes represent the key components of the established model system for the analysis of protein transport into the mammalian endoplasmic reticulum. More recently, these microsomes were also employed in cell-free systems which address questions related to protein folding and protein degradation in the ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.149
更新日期:1999-10-01 00:00:00
abstract::The mitochondrial DNA (mtDNA) encompasses two classes of functionally important sequence variants: recent pathogenic mutations and ancient adaptive polymorphisms. To rapidly and cheaply evaluate both classes of single nucleotide variants (SNVs), we have developed an integrated system in which mtDNA SNVs are analyzed b...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2010.125
更新日期:2010-10-01 00:00:00
abstract::The relationship between the structure and activity of meta- and para-hydroxylated monophenols was studied during their tyrosinase-catalysed hydroxylation and the rate-limiting steps of the reaction mechanism were identified. The para-hydroxylated substrates permit us to study the effect of a substituent (R) in the ca...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.041
更新日期:2000-04-01 00:00:00
abstract::Haptoglobin (Hpt) is known to capture circulating free hemoglobin (Hb) and bind apolipoprotein (Apo) A-I or E. Here, we report that Hb can be tightly bound by most of Hpt molecules (TB-Hpt, 80%), whereas loosely bound by a minor part of them (LB-Hpt, 20%). LB-Hpt amount was significantly increased (over 60%) in patien...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2011.033
更新日期:2011-04-01 00:00:00
abstract::Low NO concentrations synthesized by constitutively expressed NO synthases act on several signaling pathways activating transcription factors (TF), such as NF-kappaB or AP-1, and thereby influence gene expression. In contrast, during inflammatory reactions the inducible NO synthase produces NO for prolonged periods of...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2003.153
更新日期:2003-10-01 00:00:00
abstract::Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes. ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.062
更新日期:2001-03-01 00:00:00
abstract::The regulation of eukaryotic gene expression is a complicated process involving the interaction of a large number of transacting factors with specific cis-regulatory elements. DNA methylation plays a role in this scheme by acting in cis to modulate protein-DNA interactions. Several lines of evidence indicate that meth...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/bchm.1998.379.4-5.401
更新日期:1998-04-01 00:00:00
abstract::Proteomic identification of protease cleavage site specificity (PICS) is a recent proteomic approach for the easy mapping of protease subsite preferences that determines both the prime- and non-prime side specificity concurrently. Here we greatly facilitate user access by providing an automated and simple web-based da...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2011.158
更新日期:2011-11-01 00:00:00
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journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0310
更新日期:2019-03-26 00:00:00
abstract::The kinin B2 receptor, which is constitutively expressed in a large number of tissues, mediates most of the known effects of bradykinin (BK). Normally undetectable in healthy tissues, the B1 receptor is strongly over-expressed under pathological conditions. BK is an important mediator in renal homeostasis and is mainl...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.004
更新日期:2006-01-01 00:00:00
abstract::We have previously described Kunitz-type serine proteinase inhibitors purified from Bauhinia seeds. Human plasma kallikrein shows different susceptibility to those inhibitors. In this communication, we describe the interaction of human plasma kallikrein with fluorogenic and non-fluorogenic peptides based on the Bauhin...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.016
更新日期:2001-01-01 00:00:00
abstract::Cysteine cathepsins are expressed in most tissues, including the gastrointestinal tract. We demonstrated an involvement of mouse intestinal cathepsin B in extracellular matrix remodeling for regeneration from trauma. The present study aimed at elucidating roles of cysteine cathepsins in the non-traumatized gastrointes...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2014-0151
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abstract::A deletion mutant (V314) of the calcineurin A subunit was constructed using site-directed mutagenesis. Its phosphatase activity and function were then characterized. The V314 deletion significantly altered the phosphatase activity, which was more than ten times higher than that of wild-type calcineurin, the calcineuri...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.163
更新日期:1999-11-01 00:00:00
abstract::Heparan sulfate proteoglycans are obligatory for receptor binding and mitogenic activity of the basic fibroblast growth factor (bFGF). In the present study the influence of undersulfated heparan sulfate on the expression of basic fibroblast growth factor and coronary smooth muscle cell (cSMC) proliferation was investi...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1997.378.7.701
更新日期:1997-07-01 00:00:00
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journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0309
更新日期:2019-03-26 00:00:00
abstract::Only one transcript for KLK10 was identified by RT-PCR in lung tissue, whereas KLK11 expressed at least four alternative transcripts. Quantitative analysis of KLK10 and KLK11 expression levels was assessed by real-time PCR, in a cohort of 47 patients with non-small-cell lung cancer (NSCLC). Expression levels of these ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.098
更新日期:2006-06-01 00:00:00
abstract::The GAGA box of the rat serine protease inhibitor 2 (spi 2) genes not only acts as a basal promoter element, but also mediates transcriptional activation by growth hormone and interleukin-6. The GAGA box is separated from the TATA box by only 12 bp, and this close association is required for efficient transcription. H...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.127
更新日期:2000-11-01 00:00:00
abstract::A FAD and [4Fe-4S]cluster-containing enzyme from Clostridium aminobutyricum catalyses the reversible dehydration of 4-hydroxybutyryl-CoA to crotonyl-CoA which involves the cleavage of an unactivated C-H bond at the beta-carbon. Transient oxidation of the substrate to an enoxy radical by FAD might facilitate the remova...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1997.378.8.843
更新日期:1997-08-01 00:00:00
abstract::The cellular adhesion molecule E-selectin is expressed on activated endothelial cells, and is involved in the process of adherence of blood cells to vessel endothelium in inflammatory events such as atherosclerosis. In a recent study we found a Ser128Arg mutation in the EGF domain as well as a Leu554Phe mutation in th...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.082
更新日期:1999-06-01 00:00:00
abstract::The ligand-activated aryl hydrocarbon receptor (AHR) is known to modulate many genes in a highly cell-specific manner, either directly or indirectly via secondary effects. In contrast, little is known about the effects of AHR deficiency on gene expression balance. We compared the transcriptome of CD4 T cells from AHR-...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.151
更新日期:2006-09-01 00:00:00
abstract::Bacterial proteases secreted into an infected host may exhibit a wide range of pathogenic potentials. Staphylococci, in particular Staphylococcus aureus, are known to produce several extracellular proteases, including serine-, cysteine- and metalloenzymes. Their insensitivity to most human plasma protease inhibitors a...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2002.116
更新日期:2002-07-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV), or human herpesvirus 8 (HHV8) is a gammaherpesvirus and the etiological agent of Kaposi's sarcoma, primary effusion lymphoma and multicentric Castleman disease. The KSHV genome contains genes for a unique group of proteins with homology to cellular interferon regulatory ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2017-0111
更新日期:2017-07-26 00:00:00