Characterization of a new toxin from the entomopathogenic fungus Metarhizium anisopliae: the ribotoxin anisoplin.

Abstract:

:Metarhizium anisopliae is an entomopathogenic fungus relevant in biotechnology with applications like malaria vector control. Studies of its virulence factors are therefore of great interest. Fungal ribotoxins are toxic ribonucleases with extraordinary efficiency against ribosomes and suggested as potential insecticides. Here we describe this ribotoxin characteristic activity in M. anisopliae cultures. Anisoplin has been obtained as a recombinant protein and further characterized. It is structurally similar to hirsutellin A, the ribotoxin from the entomopathogen Hirsutella thompsonii. Moreover, anisoplin shows the ribonucleolytic activity typical of ribotoxins and cytotoxicity against insect cells. How Metarhizium uses this toxin and possible applications are of interest.

journal_name

Biol Chem

journal_title

Biological chemistry

authors

Olombrada M,Medina P,Budia F,Gavilanes JG,Martínez-Del-Pozo Á,García-Ortega L

doi

10.1515/hsz-2016-0119

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

135-142

issue

1

eissn

1431-6730

issn

1437-4315

pii

/j/bchm.just-accepted/hsz-2016-0119/hsz-2016-0119.

journal_volume

398

pub_type

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