Nitrosative stress and transcription.

abstract：:In a recent study we showed that two proteinases (CMS2MS2 and CMS2MS3) from Carica candamarcensis enhance mammalian cell proliferation. The aim of the present study is the determination of the primary structure of CMS2MS2 and prediction of its three-dimensional structure. The protein contains 214 residues, including t...

journal_title：Biological chemistry

authors： Gomes MT,Bemquerer MP,Lopes MT,Richardson M,Júnior SO,Salas CE

更新日期：2007-08-01 00:00:00

abstract：:Hsp70 chaperone systems play an essential role in the life cycle of many proteins not only in an hostile environment but also under normal growth conditions. In the course of evolution the diversification of functions was accompanied by an amplification of components of the Hsp70 system. Here strategies are reviewed h...

journal_title：Biological chemistry

authors： Mayer MP,Bukau B

更新日期：1998-03-01 00:00:00

abstract：:The hair follicle consists of a complex system of multiple tissue compartments that are clearly distinguishable by their morphology and type of differentiation. We have synthesized hair follicle-specific keratins from the companion layer (K6hf, K17) and the hair cortex (Ha1, Hb3, Hb6) in Escherichia coli. The assembly...

journal_title：Biological chemistry

authors： Hofmann I,Winter H,Mücke N,Langowski J,Schweizer J

更新日期：2002-09-01 00:00:00

abstract：:Many aspects of the RNA maturation leave traces in RNA sequencing data in the form of deviations from the reference genomic DNA. This includes, in particular, genomically non-encoded nucleotides and chemical modifications. The latter leave their signatures in the form of mismatches and conspicuous patterns of sequenci...

journal_title：Biological chemistry

authors： Findeiss S,Langenberger D,Stadler PF,Hoffmann S

更新日期：2011-04-01 00:00:00

abstract：:The signal recognition particle (SRP), a ubiquitous cytoplasmic ribonucleoprotein particle, plays an essential role in promoting co-translational translocation of proteins into the endoplasmic reticulum. Here, we summarise recent progress made in the understanding of two essential SRP functions: the signal recognition...

journal_title：Biological chemistry

authors： Bui N,Strub K

更新日期：1999-02-01 00:00:00

abstract：:The infectivity and pathogenicity of influenza virus are primarily determined by host cellular trypsin-type processing proteases which cleave the viral membrane fusion glycoprotein hemagglutinin (HA). Therefore the distribution of the processing protease is a major determinant of the infectious organ tropism. The comm...

journal_title：Biological chemistry

authors： Yao D,Chen Y,Kuwajima M,Shiota M,Kido H

更新日期：2004-06-01 00:00:00

abstract：:Cells need to process multifaceted external cues to steer their dynamic behavior. To efficiently perform this task, cells implement several exploratory mechanisms to actively sample their environment. In particular, cells can use exploratory actin-based cell protrusions and contractions to engage and squeeze the envir...

journal_title：Biological chemistry

authors： Nalbant P,Dehmelt L

更新日期：2018-07-26 00:00:00

abstract：:The influence of the cellular milieu, a complex and crowded solvent, is often neglected when biomolecular structure and function are studied in vitro. To mimic the cellular environment, crowding effects are commonly induced in vitro using artificial crowding agents like Ficoll or dextran. However, it is unclear if suc...

journal_title：Biological chemistry

authors： Gnutt D,Ebbinghaus S

更新日期：2016-01-01 00:00:00

abstract：:Trypsin and chymotrypsin have similar tertiary structures, although very different substrate specificities. Trypsin hydrolyzes peptides at Lys/Arg residues while chymotrypsin recognizes large hydrophobic residues. Recent work has shown that trypsin is not converted into a protease with chymotrypsin-like activity when ...

journal_title：Biological chemistry

authors： Hedstrom L

更新日期：1996-07-01 00:00:00

abstract：:To elucidate the structures and dynamics of membrane proteins, highly advanced biophysical methods have been developed that often require significant resources, both for sample preparation and experimental analyses. For very complex systems, such as membrane transporters, ion channels or G-protein coupled receptors (G...

journal_title：Biological chemistry

authors： Rose-Sperling D,Tran MA,Lauth LM,Goretzki B,Hellmich UA

更新日期：2019-09-25 00:00:00

abstract：:To exploit the B-lymphocyte antigen-CD20 binding capacity of the Ibritumomab tiuxetan (IBTN) monoclonal antibody (mAb) for imaging, the over-expression of B cells in non-Hodgkin's lymphoma (NHL) (a myeloproliferative disorder of the lymphatic system) was investigated. In the current investigation, we present the label...

journal_title：Biological chemistry

authors： Shah SQ,Khan AU,Khan MR

更新日期：2012-01-01 00:00:00

abstract：:Cytochrome c oxidase from bovine heart contains seven binding sites for ATP or ADP and three additional for ADP only, as concluded from competition equilibrium dialysis binding studies. The isolated enzyme contains bound cholate which, in contrast to bound ATP, is only slowly exchanged by ADP (or ATP). The kinetics of...

journal_title：Biological chemistry

authors： Napiwotzki J,Kadenbach B

更新日期：1998-03-01 00:00:00

abstract：:Studies of extracellular matrix (ECM) biology in the nervous system have mainly focused on laminin, fibronectin and tenascin-R, proteins that are present during nervous system development and normal function. However, during disease, fibrin, which physiologically is not present in the nervous tissue, is detected at ne...

journal_title：Biological chemistry

authors： Akassoglou K,Strickland S

更新日期：2002-01-01 00:00:00

abstract：:The sphingolipid sphingosine-1-phosphate (S1P) has various functions in immune cell biology, regulating survival, proliferation, and, most prominently, migration. S1P couples to five G protein-coupled receptors (S1PR1-5) to transduce its effects on immune cell function. Expression of S1PR4 is restricted to immune cell...

journal_title：Biological chemistry

authors： Dillmann C,Mora J,Olesch C,Brüne B,Weigert A

更新日期：2015-06-01 00:00:00

abstract：:Loss of genetic material from chromosome 3p21.3 is one of the most common and earliest events in the pathogenesis of lung cancer and many other solid tumors. The chromosomal area 3p21.3 is thought to harbor at least one important tumor suppressor gene, which, despite many years of investigation, has remained elusive. ...

journal_title：Biological chemistry

authors： Pfeifer GP,Yoon JH,Liu L,Tommasi S,Wilczynski SP,Dammann R

更新日期：2002-06-01 00:00:00

abstract：:One of the control mechanisms of cathepsin B biosynthesis and trafficking operates through alternative splicing of pre-mRNA. An mRNA lacking exon 2 is more efficiently translated than that containing all exons, and may be responsible for elevated biosynthesis and enzyme routing to the extracellular space, with critica...

journal_title：Biological chemistry

authors： Baici A,Müntener K,Willimann A,Zwicky R

更新日期：2006-08-01 00:00:00

abstract：:The ligand-activated aryl hydrocarbon receptor (AHR) is known to modulate many genes in a highly cell-specific manner, either directly or indirectly via secondary effects. In contrast, little is known about the effects of AHR deficiency on gene expression balance. We compared the transcriptome of CD4 T cells from AHR-...

journal_title：Biological chemistry

authors： Frericks M,Temchura VV,Majora M,Stutte S,Esser C

更新日期：2006-09-01 00:00:00

abstract：:To facilitate evolutionary and forensic studies of DNA polymorphisms on the Y chromosome, we devised a multiplex amplification procedure for short-tandem-repeat (STR) loci. Four tetranucleotide STR loci (DYS19, DYS390, DYS391, and DYS393) were simultaneously amplified with FAM-labeled primers and genotypes were determ...

journal_title：Biological chemistry

authors： Redd AJ,Clifford SL,Stoneking M

更新日期：1997-08-01 00:00:00

abstract：:Abstract Hairpin ribozymes occur naturally only in the satellite RNAs of tobacco ringspot virus (TRsV), chicory yellow mottle virus (CYMoV) and arabis mosaic virus (ArMV). The catalytic centre of the predominantly studied sTRsV hairpin ribozyme, and of sArMV is organised around a four-way helical junction. We show her...

journal_title：Biological chemistry

authors： Bajaj P,Steger G,Hammann C

更新日期：2011-07-01 00:00:00

abstract：:Major vault protein (MVP) is the predominant member of a large cytosolic ribonucleoprotein particle, termed vault. We have previously shown that MVP derived from electric ray electric organ becomes phosphorylated by protein kinase C in vitro and by tyrosine kinase in vivo. Here we show that MVP from two mammalian cell...

journal_title：Biological chemistry

authors： Ehrnsperger C,Volknandt W

更新日期：2001-10-01 00:00:00

abstract：:Family with sequence similarity 134, Member B (FAM134B), is a cis-Golgi transmembrane protein that is known to be necessary for the long-term survival of nociceptive and autonomic ganglion neurons. Recent work has shown that FAM134B plays a pivotal role in autophagy-mediated turnover of endoplasmic reticulum (ER) memb...

journal_title：Biological chemistry

authors： Cai M,Chen J,Yu C,Xi L,Jiang Q,Wang Y,Wang X

更新日期：2019-03-26 00:00:00

abstract：:Many neurodegenerative disorders and syndromes are associated with an excessive generation of reactive oxygen species (ROS) and oxidative stress. The pathways to nerve cell death induced by diverse potential neurotoxins such as peptides, excitatory amino acids, cytokines or synthetic drugs commonly share oxidative dow...

journal_title：Biological chemistry

authors： Behl C,Moosmann B

更新日期：2002-03-01 00:00:00

abstract：:A high-resolution crystallographic structure determination of a protein-ligand complex is generally accepted as the 'gold standard' for structure-based drug design, yet the relationship between structure and affinity is neither obvious nor straightforward. Here we analyze the interactions of a series of serine protein...

journal_title：Biological chemistry

authors： Tziridis A,Rauh D,Neumann P,Kolenko P,Menzel A,Bräuer U,Ursel C,Steinmetzer P,Stürzebecher J,Schweinitz A,Steinmetzer T,Stubbs MT

更新日期：2014-07-01 00:00:00

abstract：:The 15 human kallikrein-related peptidases (KLKs) are clinically important biomarkers and therapeutic targets of interest in inflammation, cancer, and neurodegenerative disease. KLKs are secreted as inactive pro-forms (pro-KLKs) that are activated extracellularly by specific proteolytic release of their amino-terminal...

journal_title：Biological chemistry

authors： Yoon H,Blaber SI,Li W,Scarisbrick IA,Blaber M

更新日期：2013-01-01 00:00:00

abstract：:Mammalian tyrosinases are key enzymes of melanin formation. Their native forms undergo complex maturation and sorting processes before being integrated into the melanosomal membrane, which greatly complicates their heterologous expression in other cell types. In the present work, we constructed several differently tru...

journal_title：Biological chemistry

authors： Cordes P,Sun W,Wolber R,Kolbe L,Klebe G,Röhm KH

更新日期：2013-05-01 00:00:00

abstract：:Chitinase-like proteins (CLPs) are lectins combining properties of cytokines and growth factors. Human CLPs include YKL-40, YKL-39 and SI-CLP that are secreted by cancer cells, macrophages, neutrophils, synoviocytes, chondrocytes and other cells. The best investigated CLP in cancer is YKL-40. Serum and plasma levels o...

journal_title：Biological chemistry

authors： Kzhyshkowska J,Yin S,Liu T,Riabov V,Mitrofanova I

更新日期：2016-03-01 00:00:00

abstract：:Based on studies presented here and other published experiments performed with surviving tissue preparations, with transfected cells and with cells that constitutively express the human angiotensin I converting enzyme ACE and B2 receptors, we concluded the following: ACE inhibitors and other endogenous peptides that r...

journal_title：Biological chemistry

authors： Erdös EG,Marcic BM

更新日期：2001-01-01 00:00:00

abstract：:The cellular adhesion molecule E-selectin is expressed on activated endothelial cells, and is involved in the process of adherence of blood cells to vessel endothelium in inflammatory events such as atherosclerosis. In a recent study we found a Ser128Arg mutation in the EGF domain as well as a Leu554Phe mutation in th...

journal_title：Biological chemistry

authors： Wenzel K,Stahn R,Speer A,Denner K,Gläser C,Affeldt M,Moobed M,Scheer A,Baumann G,Felix SB

更新日期：1999-06-01 00:00:00

abstract：:The three-dimensional structure of pterin-4a-carbinolamine dehydratase (PCD) from Pseudomonas aeruginosa has been solved. Based on this we have investigated the roles of putative active center residues through functional replacement by site-directed mutagenesis. Three histidines, His73, His74 and His91, appear to be i...

journal_title：Biological chemistry

authors： Köster S,Stier G,Kubasch N,Curtius HC,Ghisla S

更新日期：1998-12-01 00:00:00

abstract：:The ATP-binding cassette (ABC) transporter genes represent the largest family of transporters and these genes are abundant in the genome of all vertebrates. Through analysis of the genome sequence databases we have characterized the full complement of ABC genes from several mammals and other vertebrates. Multiple gene...

journal_title：Biological chemistry

authors： Moitra K,Dean M

更新日期：2011-01-01 00:00:00