Oxidative nerve cell death in Alzheimer's disease and stroke: antioxidants as neuroprotective compounds.

abstract：:Human pro-matrix metalloproteinase 3 (proMMP-3) lacking the N-terminal 34 amino acids and the C-terminal hemopexin-like domain was expressed in E. coli and used to investigate the process of proenzyme activation and its interaction with an endogenous inhibitor TIMP-1 during activation. The truncated precursor was puri...

journal_title：Biological chemistry

authors： Suzuki K,Kan CC,Hung W,Gehring MR,Brew K,Nagase H

更新日期：1998-02-01 00:00:00

abstract：:RIO1 is an essential gene that encodes a protein serine kinase and is transcribed constitutively at a very low level. Transcriptional activation of RIO1 dispenses with a canonical TATA box as well as with classical transactivators or specific DNA-binding factors. Instead, a dG-dC-rich sequence element, that is located...

journal_title：Biological chemistry

authors： Angermayr M,Schwerdffeger K,Bandlow W

更新日期：2003-09-01 00:00:00

abstract：:Human kallikrein-related peptidases (KLKs), including the well-known prostate cancer biomarker, prostate-specific antigen (PSA-KLK3), along with 14 other serine proteinase KLK family members are now known to regulate cells by cleaving and activating members of the G-protein-coupled proteinase-activated receptor (PAR) ...

journal_title：Biological chemistry

authors： Hollenberg MD

更新日期：2014-09-01 00:00:00

abstract：:Metarhizium anisopliae is an entomopathogenic fungus relevant in biotechnology with applications like malaria vector control. Studies of its virulence factors are therefore of great interest. Fungal ribotoxins are toxic ribonucleases with extraordinary efficiency against ribosomes and suggested as potential insecticid...

journal_title：Biological chemistry

authors： Olombrada M,Medina P,Budia F,Gavilanes JG,Martínez-Del-Pozo Á,García-Ortega L

更新日期：2017-01-01 00:00:00

abstract：:The formation of reactive oxygen species (ROS) can be induced by xenobiotic substances, such as redox cycling molecules, but also by endogenous substances such as hormones and cytokines. Recent research shows the importance of ROS in cellular signaling. Here, the signaling pathways of the two blood pressure-regulating...

journal_title：Biological chemistry

authors： Queisser N,Fazeli G,Schupp N

更新日期：2010-11-01 00:00:00

abstract：:Cytokinins are essential plant hormones that regulate numerous physiological processes. Recently, a protein was identified in mung bean (Vigna radiata) and characterized as a cytokinin-specific binding protein (VrCSBP). Fluorescence correlation spectroscopy was used to investigate the interaction between VrCSBP and it...

journal_title：Biological chemistry

authors： Zawadzki P,Slósarek G,Boryski J,Wojtaszek P

更新日期：2010-01-01 00:00:00

abstract：:The hair follicle consists of a complex system of multiple tissue compartments that are clearly distinguishable by their morphology and type of differentiation. We have synthesized hair follicle-specific keratins from the companion layer (K6hf, K17) and the hair cortex (Ha1, Hb3, Hb6) in Escherichia coli. The assembly...

journal_title：Biological chemistry

authors： Hofmann I,Winter H,Mücke N,Langowski J,Schweizer J

更新日期：2002-09-01 00:00:00

abstract：:The major enzymatic activity of the ribosome is the catalysis of peptide bond formation. The active site -- the peptidyl transferase center -- is composed of ribosomal RNA (rRNA), and interactions between rRNA and the reactants, peptidyl-tRNA and aminoacyl-tRNA, are crucial for the reaction to proceed rapidly and effi...

journal_title：Biological chemistry

authors： Beringer M,Rodnina MV

更新日期：2007-07-01 00:00:00

abstract：:Bradykinin (BK) is a vasorelaxant, algesic and inflammatory agent. Angiotensin II (AngII) is known to control vascular tone and promote growth, inflammation and artherogenesis. There is evidence for cross talking between BK and AngII receptors. Therefore, the effect of lack of kinin receptors was assessed in mice with...

journal_title：Biological chemistry

authors： Rodrigues ES,Martin RP,Felipe SA,Bader M,Oliveira SM,Shimuta SI

更新日期：2009-09-01 00:00:00

abstract：:Neuritic plaques of Alzheimer patients are composed of multiple protein components. Among them, the amyloid beta-peptides (Abeta) 1-40/42 and further N- and C-terminally modified fragments of Abeta are highly abundant. Most prominent are the isoaspartate (isoAsp)-Abeta peptides and pyroglutamyl (pGlu)-Abeta. While pGl...

journal_title：Biological chemistry

authors： Böhme L,Hoffmann T,Manhart S,Wolf R,Demuth HU

更新日期：2008-08-01 00:00:00

abstract：:During the last years, the use of therapeutic glycoproteins has increased strikingly. Glycosylation of recombinant glycoproteins is of major importance in biotechnology, as the glycan composition of recombinant glycoproteins impacts their pharmacological properties. The terminal position of N-linked complex glycans in...

journal_title：Biological chemistry

authors： Werner A,Horstkorte R,Glanz D,Biskup K,Blanchard V,Berger M,Bork K

更新日期：2012-08-01 00:00:00

abstract：:We present a novel strategy, termed CISTEM, which allows direct in vivo screening of polypeptides displayed on the surface of E. coli cells by a combination of ligand-mediated protection and phage-mediated selection. The effectiveness of this new approach was demonstrated by displaying the T7.tag on the surface of E. ...

journal_title：Biological chemistry

authors： Camaj P,Hirsh AE,Schmidt W,Meinke A,von Gabain A

更新日期：2001-12-01 00:00:00

abstract：:N-acyl homoserine lactones (AHLs) are quorum sensing (QS) signal molecules used by most Gram-negative pathogenic bacteria. In this article the lactonase activity of the preparations based on hexahistidine-tagged organophosphorus hydrolase (His6-OPH) towards AHLs was studied. Initially, three of the most interesting β-...

journal_title：Biological chemistry

authors： Aslanli A,Lyagin I,Efremenko E

更新日期：2018-07-26 00:00:00

abstract：:CYP20A1 is a well-conserved member of the human cytochrome P450 enzyme family for which no endogenous or xenobiotic substrate is known. We have recently shown that this enzyme has moderate activity towards two proluciferin probe substrates. In order to facilitate the search for physiological substrates we have tested ...

journal_title：Biological chemistry

authors： Durairaj P,Fan L,Sharma SS,Jie Z,Bureik M

更新日期：2020-02-25 00:00:00

abstract：:Angiotensin-converting enzyme (ACE) plays an important role in blood pressure control. ACE also has effects on renal function, reproduction, hematopoiesis, and several aspects of the immune response. ACE 10/10 mice overexpress ACE in monocytic cells; macrophages from ACE 10/10 mice demonstrate increased polarization t...

journal_title：Biological chemistry

authors： Bernstein KE,Gonzalez-Villalobos RA,Giani JF,Shah K,Bernstein E,Janjulia T,Koronyo Y,Shi PD,Koronyo-Hamaoui M,Fuchs S,Shen XZ

更新日期：2014-10-01 00:00:00

abstract：:Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) is a membrane-anchored docking protein that has been shown to play an important role in linking FGF, nerve growth factor (NGF) and glial cell-derived neurotrophic factor (GDNF) receptors with the Ras/mitogen-activated protein (MAP) kinase signaling cascade. He...

journal_title：Biological chemistry

authors： Wu Y,Chen Z,Ullrich A

更新日期：2003-08-01 00:00:00

abstract：:Amphetamine ('Speed'), methamphetamine ('Ice') and its congener 3,4-methylenedioxymethamphetamine (MDMA; 'Ecstasy') are illicit drugs abused worldwide for their euphoric and stimulant effects. Despite compelling evidence for chronic MDMA neurotoxicity in animal models, the physiological consequences of such toxicity i...

journal_title：Biological chemistry

authors： Steinkellner T,Freissmuth M,Sitte HH,Montgomery T

更新日期：2011-01-01 00:00:00

abstract：:Loss of genetic material from chromosome 3p21.3 is one of the most common and earliest events in the pathogenesis of lung cancer and many other solid tumors. The chromosomal area 3p21.3 is thought to harbor at least one important tumor suppressor gene, which, despite many years of investigation, has remained elusive. ...

journal_title：Biological chemistry

authors： Pfeifer GP,Yoon JH,Liu L,Tommasi S,Wilczynski SP,Dammann R

更新日期：2002-06-01 00:00:00

abstract：:The cloning of a Ca2+(0)-sensing receptor (CaR) from parathyroid and kidney, coupled with the identification of inherited disorders in Ca2+(0)-sensing resulting from inactivating or activating mutations of this CaR, has provided substantial insights into how Ca2+(0) is regulated at a systemic level. The CaR plays a ce...

journal_title：Biological chemistry

authors： Chattopadhyay N,Vassilev PM,Brown EM

更新日期：1997-08-01 00:00:00

abstract：:Tissue kallikrein KLK1 and the kallikrein-related peptidases KLK2-15 are a subfamily of serine proteases that have defined or proposed roles in a range of central nervous system (CNS) and non-CNS pathologies. To further understand their potential activity in multiple sclerosis (MS), serum levels of KLK1, 6, 7, 8 and 1...

journal_title：Biological chemistry

authors： Scarisbrick IA,Linbo R,Vandell AG,Keegan M,Blaber SI,Blaber M,Sneve D,Lucchinetti CF,Rodriguez M,Diamandis EP

更新日期：2008-06-01 00:00:00

abstract：:The accumulation of cholesterol is a general feature of cancer tissue, and recent evidence suggests that cholesterol plays critical roles in the progression of cancers, including breast, prostate, and colorectal cancers. The dysregulation of metabolic pathways, including those involved in cholesterol biosynthesis, is ...

journal_title：Biological chemistry

authors： Murai T

更新日期：2015-01-01 00:00:00

abstract：:The cell, as a molecular system, is often interpreted in terms of complex clockworks, and the design charts of mechanical and electrical engineering are assumed to provide adequate approximations for the description of cellular organization. However, a growing body of experimental evidence obtained through the observa...

journal_title：Biological chemistry

authors： Kurakin A

更新日期：2005-03-01 00:00:00

abstract：:The kinin B2 receptor, which is constitutively expressed in a large number of tissues, mediates most of the known effects of bradykinin (BK). Normally undetectable in healthy tissues, the B1 receptor is strongly over-expressed under pathological conditions. BK is an important mediator in renal homeostasis and is mainl...

journal_title：Biological chemistry

authors： Bachvarov D,Bachvarova M,Koumangaye R,Klein J,Pesquero JB,Neau E,Bader M,Schanstra JP,Bascands JL

更新日期：2006-01-01 00:00:00

abstract：:Rasal is a modular multi-domain protein of the GTPase-activating protein 1 (GAP1) family; its four known members, GAP1m, Rasal, GAP1IP4BP and Capri, have a Ras GTPase-activating domain (RasGAP). This domain supports the intrinsically slow GTPase activity of Ras by actively participating in the catalytic reaction. In t...

journal_title：Biological chemistry

authors： Cuellar J,Valpuesta JM,Wittinghofer A,Sot B

更新日期：2017-12-20 00:00:00

abstract：:Cysteine proteases of the malaria parasite Plasmodium falciparum, known as falcipains, are promising targets for antimalarial chemotherapy. We evaluated cultured parasites for the stage-specific expression of cysteine proteases and sensitivity to cysteine protease inhibitors. Protease activity and inhibitor sensitivit...

journal_title：Biological chemistry

authors： Shenai BR,Semenov AV,Rosenthal PJ

更新日期：2002-05-01 00:00:00

abstract：:The enzymatic properties of glycosylphosphatidylinositol-specific phospholipase D (EC 3.1.4.50) were characterized using a 6,000-fold purified enzyme. This was obtained in 100 microg amounts from human serum with a recovery of 35%. Pure alkaline phosphatase containing one anchor moiety per molecule was used as substra...

journal_title：Biological chemistry

authors： Rhode H,Schulze M,Cumme GA,Göhlert A,Blume E,Bublitz R,Schilling K,Horn A

更新日期：2000-05-01 00:00:00

abstract：:Coxsackievirus B3 (CVB-3) is a plus-strand RNA virus that is believed to be the most common causal agent of viral myocarditis. Since no specific treatment for CVB-3 infections is available to date, we and others have recently started to develop RNA interference (RNAi) approaches to prevent virus propagation. Here we d...

journal_title：Biological chemistry

authors： Werk D,Schubert S,Lindig V,Grunert HP,Zeichhardt H,Erdmann VA,Kurreck J

更新日期：2005-09-01 00:00:00

abstract：:The transcription factor E2F1 plays a decisive role in the G1/S and G2/M checkpoint transitions of proliferating cells. Because cells are arrested at these checkpoints after heat shock it was of interest to test heat shock effects on E2F1 activity. In human A549 cells, heat shock (44 degrees C, 30 min) caused an immed...

journal_title：Biological chemistry

authors： Gerullis D,Rensing L,Beyersmann D

更新日期：2003-01-01 00:00:00

abstract：:Bacterial proteases secreted into an infected host may exhibit a wide range of pathogenic potentials. Staphylococci, in particular Staphylococcus aureus, are known to produce several extracellular proteases, including serine-, cysteine- and metalloenzymes. Their insensitivity to most human plasma protease inhibitors a...

journal_title：Biological chemistry

authors： Dubin G

更新日期：2002-07-01 00:00:00

abstract：:Two members of the nuclear receptor superfamily, EcR (ecdysteroid receptor protein) and Usp (Ultraspiracle), heterodimerize to form a functional receptor for the steroid hormone 20-hydroxyecdysone and thus enable it to coordinate morphogenetic events during insect metamorphosis. N-terminally His-tagged Usp was overexp...

journal_title：Biological chemistry

authors： Rymarczyk G,Grad I,Rusek A,Oświecimska-Rusin K,Niedziela-Majka A,Kochman M,Ozyhar A

更新日期：2003-01-01 00:00:00