Multiplex DNA typing of short-tandem-repeat loci on the Y chromosome.

abstract：:The conversion of cellular prion protein (PrP(C)) into its pathological isoform (PrP(Sc)) conveys an increase in hydrophobicity and induces a partial resistance to proteinase K (PK). Interestingly, co-incubation with high copper ion concentrations also modifies the solubility of PrP(c) and induces a partial PK resista...

journal_title：Biological chemistry

authors： Kuczius T,Buschmann A,Zhang W,Karch H,Becker K,Peters G,Groschup MH

更新日期：2004-08-01 00:00:00

abstract：:Five moeA mutants were generated by replacing some conserved amino acids of MoeA by site-directed mutagenesis. The mutants were assayed for the ability to restore in vivo nitrate reductase activity of the moeA mutant Escherichia coli JRG97 and in vitro Neurospora crassa nit-1 nitrate reductase activity. The replacemen...

journal_title：Biological chemistry

authors： Sandu C,Brandsch R

更新日期：2002-02-01 00:00:00

abstract：:Abstract The homeostasis and atheroprotective function of bilirubin could be an appealing model to investigate one of the many physiologic functions of the human aryl hydrocarbon receptor (AhR). Several clinical and epidemiological studies have been carried out on key enzymes generating and eliminating bilirubin (heme...

journal_title：Biological chemistry

authors： Bock KW,Köhle C

更新日期：2010-06-01 00:00:00

abstract：:Kallikrein-related peptidase 2 (KLK2) degrades insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) in vitro. IGFBP-3 forms complexes with IGFs, preventing them from binding to their receptors and stimulating cell proliferation and survival. IGF-independent actions have also been described for IGFBP-3. The deg...

journal_title：Biological chemistry

authors： Hekim C,Riipi T,Weisell J,Närvänen A,Koistinen R,Stenman UH,Koistinen H

更新日期：2010-04-01 00:00:00

abstract：:Calpain is a calcium-activated non lysosomal neutral thiol protease (EC 3.4.22.17) present in a wide variety of eukaryotic cells. Calpain is usually present as an inactive form and is activated by calcium ions and phospholipids. The ability of calpain to alter, by limited proteolysis, the activity or function of numer...

journal_title：Biological chemistry

authors： Yajima Y,Kawashima S

更新日期：2002-05-01 00:00:00

abstract：:The regulation of eukaryotic gene expression is a complicated process involving the interaction of a large number of transacting factors with specific cis-regulatory elements. DNA methylation plays a role in this scheme by acting in cis to modulate protein-DNA interactions. Several lines of evidence indicate that meth...

journal_title：Biological chemistry

authors： Bergman Y,Mostoslavsky R

更新日期：1998-04-01 00:00:00

abstract：:In order to study the role of N-glycans in the ER-associated degradation of unassembled immunoglobulin light (Ig L) chains, we introduced N-glycan acceptor sites into the variable domain of the murine Ig L chain kappaNS1, which is unfolded in unassembled molecules. We investigated the fate of kappaNS1 glycosylated at ...

journal_title：Biological chemistry

authors： Chillarón J,Adán C,Haas IG

更新日期：2000-12-01 00:00:00

abstract：:The purpose of this work was to study the regulative role of the glutamate receptor found earlier in the brain mitochondria. In the present work a glutamate-dependent signaling system with similar features was detected in mitochondria of the heart. The glutamate-dependent signaling system in the heart mitochondria was...

journal_title：Biological chemistry

authors： Selin AA,Lobysheva NV,Nesterov SV,Skorobogatova YA,Byvshev IM,Pavlik LL,Mikheeva IB,Moshkov DA,Yaguzhinsky LS,Nartsissov YR

更新日期：2016-05-01 00:00:00

abstract：:Animal cells release traces of material onto glass or silicon surfaces during adhesion and migration. This little studied phenomenon is a widespread and normal concomitant of cell migration. The paper introduces the study of such material. The traces can be visualised by different microscopic techniques (e.g. TIRF, IR...

journal_title：Biological chemistry

authors： Fuhr G,Richter E,Zimmermann H,Hitzler H,Niehus H,Hagedorn R

更新日期：1998-08-01 00:00:00

abstract：:Bacterial infections often upregulate the plasma kinin-forming cascade of the host (the 'contact system') which is triggered by adsorption of high molecular mass kininogen (HK), coagulation factor XII (FXII) and prekallikrein (pHPK) on the host or pathogen cell surfaces. A possible activation of the contact system upo...

journal_title：Biological chemistry

authors： Karkowska-Kuleta J,Kozik A,Rapala-Kozik M

更新日期：2010-01-01 00:00:00

abstract：:The hypoxia-inducible factor-1 (HIF-1) is a basic-helix-loop-helix (bHLH) heterodimeric transcription factor activated by reductions in oxygen concentration (hypoxia). Activated HIF-1 upregulates expression of genes involved in the adaptation of higher organisms to hypoxic conditions, caused by e.g. high altitude, ane...

journal_title：Biological chemistry

authors： Wenger RH,Gassmann M

更新日期：1997-07-01 00:00:00

abstract：:Upon activation of the B cell antigen receptor (BCR), the spleen tyrosine kinase (Syk) and the Src family kinase Lyn phosphorylate tyrosines of the immunoreceptor tyrosine-based activation motif (ITAM) of Igα and Igβ which further serve as binding sites for the SH2 domains of these kinases. Using a synthetic biology a...

journal_title：Biological chemistry

authors： Kulathu Y,Zuern C,Yang J,Reth M

更新日期：2019-03-26 00:00:00

abstract：:The enzymatic properties of glycosylphosphatidylinositol-specific phospholipase D (EC 3.1.4.50) were characterized using a 6,000-fold purified enzyme. This was obtained in 100 microg amounts from human serum with a recovery of 35%. Pure alkaline phosphatase containing one anchor moiety per molecule was used as substra...

journal_title：Biological chemistry

authors： Rhode H,Schulze M,Cumme GA,Göhlert A,Blume E,Bublitz R,Schilling K,Horn A

更新日期：2000-05-01 00:00:00

abstract：:Maintenance of cellular redox control is pivotal for normal cellular functions and cell fate decisions including cell death. Among the key cellular redox systems in mammals, the glutathione peroxidase (GPX) family of proteins is the largest conferring multifaceted functions and affecting virtually all cellular process...

journal_title：Biological chemistry

authors： Buday K,Conrad M

更新日期：2020-10-22 00:00:00

abstract：:Human septins comprise a family of 13 genes that encode for >30 protein isoforms with ubiquitous and tissue-specific expressions. Septins are GTP-binding proteins that assemble into higher-order oligomers and filamentous polymers, which associate with cell membranes and the cytoskeleton. In the last decade, much progr...

journal_title：Biological chemistry

authors： Dolat L,Hu Q,Spiliotis ET

更新日期：2014-02-01 00:00:00

abstract：:Many aspects of the RNA maturation leave traces in RNA sequencing data in the form of deviations from the reference genomic DNA. This includes, in particular, genomically non-encoded nucleotides and chemical modifications. The latter leave their signatures in the form of mismatches and conspicuous patterns of sequenci...

journal_title：Biological chemistry

authors： Findeiss S,Langenberger D,Stadler PF,Hoffmann S

更新日期：2011-04-01 00:00:00

abstract：:The mitochondrial DNA (mtDNA) encompasses two classes of functionally important sequence variants: recent pathogenic mutations and ancient adaptive polymorphisms. To rapidly and cheaply evaluate both classes of single nucleotide variants (SNVs), we have developed an integrated system in which mtDNA SNVs are analyzed b...

journal_title：Biological chemistry

authors： Poole JC,Procaccio V,Brandon MC,Merrick G,Wallace DC

更新日期：2010-10-01 00:00:00

abstract：:Extreme thermophiles produce unusually long polyamines, including the linear caldopentamine (Cdp) and the branched pentamine tetrakis(3-aminopropyl)-ammonium (Taa), with the latter containing a central quaternary ammonium moiety. Here we compare the interaction of these two pentamines with RNA by studying the heat den...

journal_title：Biological chemistry

authors： Hayrapetyan A,Grosjean H,Helm M

更新日期：2009-09-01 00:00:00

abstract：:Structural and functional characterization of integral membrane proteins in a bilayer environment is strongly hampered by the requirement of detergents for solubilization and subsequent purification, as detergents commonly affect their structure and/or activity. Here, we describe a rapid procedure with minimal exposur...

journal_title：Biological chemistry

authors： Shirzad-Wasei N,van Oostrum J,Bovee-Geurts PH,Kusters LJ,Bosman GJ,DeGrip WJ

更新日期：2015-08-01 00:00:00

abstract：:This review addresses many of the chemical aspects of nitrosative stress mediated by N2O3. From a cellular perspective, N2O3 and the resulting reactive nitrogen oxide species target specific motifs such as thiols, lysine active sites, and zinc fingers and is dependant upon both the rates of production as well as consu...

journal_title：Biological chemistry

authors： Ridnour LA,Thomas DD,Mancardi D,Espey MG,Miranda KM,Paolocci N,Feelisch M,Fukuto J,Wink DA

更新日期：2004-01-01 00:00:00

abstract：:The lysosomal lumen contains numerous acidic hydrolases involved in the degradation of carbohydrates, lipids, proteins, and nucleic acids, which are basic cell components that turn over continuously within the cell and/or are ingested from outside of the cell. Deficiency in almost any of these hydrolases causes accumu...

journal_title：Biological chemistry

authors： Golabek AA,Kida E

更新日期：2006-08-01 00:00:00

abstract：:A water-soluble [18O]-labeled endoperoxide derived from N,N'-di(2,3-dihydroxypropyl)-1,4-naphthalene-dipropanamide (DHPN18O2) has been shown to act as a clean chemical source of [18O]-labeled molecular singlet oxygen. This allows the assessment of the singlet oxygen (1O2) reactivity toward biological targets such as D...

journal_title：Biological chemistry

authors： Martinez GR,Medeiros MH,Ravanat JL,Cadet J,Di Mascio P

更新日期：2002-03-01 00:00:00

abstract：:Disruption of the plasma membrane poses deadly threat to eukaryotic cells and survival requires a rapid membrane repair system. Recent evidence reveal various plasma membrane repair mechanisms, which are required for cells to cope with membrane lesions including membrane fusion and replacement strategies, remodeling o...

journal_title：Biological chemistry

authors： Boye TL,Nylandsted J

更新日期：2016-10-01 00:00:00

abstract：:The evolution of a highly developed nervous system is mirrored by the ability of individual neurons to develop increased morphological complexity. As microtubules (MTs) are crucially involved in neuronal development, we tested the hypothesis that the evolution of complexity is driven by an increasing capacity of the M...

journal_title：Biological chemistry

authors： Trushina NI,Mulkidjanian AY,Brandt R

更新日期：2019-08-27 00:00:00

abstract：:Oncogenic transformation is dependent on activated membrane-associated NADPH oxidase (NOX). However, the resultant extracellular superoxide anions are also driving the NO/peroxynitrite and the HOCl pathway, which eliminates NOX-expressing transformed cells through selective apoptosis induction. Tumor progression is de...

journal_title：Biological chemistry

authors： Böhm B,Heinzelmann S,Motz M,Bauer G

更新日期：2015-12-01 00:00:00

abstract：:The heterodimer consisting of ecdysteroid receptor (EcR) and ultraspiracle (USP), both of which are members of the nuclear receptor superfamily, is considered to be the functional ecdysteroid receptor. Here we analyzed the subcellular distribution of EcR and USP fused to fluorescent proteins. The experiments were carr...

journal_title：Biological chemistry

authors： Nieva C,Gwóźdź T,Dutko-Gwóźdź J,Wiedenmann J,Spindler-Barth M,Wieczorek E,Dobrucki J,Duś D,Henrich V,Ozyhar A,Spindler KD

更新日期：2005-05-01 00:00:00

abstract：:Knockout mice are the gold standard to probe for the role of a specific protease within the interacting network of proteases, substrates, and inhibitors. This proteolytic network, or protease web, determines cell signaling and organ homeostasis. Therefore, protease deficiency or inhibition is intrinsically tied to alt...

journal_title：Biological chemistry

authors： Krüger A

更新日期：2009-02-01 00:00:00

abstract：:Plastids originated from an endosymbiotic event between an early eukaryotic host cell and an ancestor of today's cyanobacteria. During the events by which the engulfed endosymbiont was transformed into a permanent organelle, many genes were transferred from the plastidal genome to the nucleus of the host cell. Protein...

journal_title：Biological chemistry

authors： Vothknecht UC,Soll J

更新日期：2000-09-01 00:00:00

abstract：:Arginine deiminase (ADI) is a potential antitumor agent for the arginine deprivation treatment of L-arginine auxotrophic tumors. The optimum pH of ADI varies significantly, yet little is known about the origin of this variety. Here, Pseudomonas aeruginosa ADI (PaADI), an enzyme that functions only at acidic pH, was ut...

journal_title：Biological chemistry

authors： Ding H,Liu H,Yin Y,Ding Y,Jia Y,Chen Q,Zou G,Zheng Z

更新日期：2012-09-01 00:00:00

abstract：:Abstract Hairpin ribozymes occur naturally only in the satellite RNAs of tobacco ringspot virus (TRsV), chicory yellow mottle virus (CYMoV) and arabis mosaic virus (ArMV). The catalytic centre of the predominantly studied sTRsV hairpin ribozyme, and of sArMV is organised around a four-way helical junction. We show her...

journal_title：Biological chemistry

authors： Bajaj P,Steger G,Hammann C

更新日期：2011-07-01 00:00:00