Abstract:
:MHC encoded DM heterodimers and classical MHC class II complexes meet in an endosomal/lysosomal compartment where DM heterodimers support peptide loading of MHC class II. Studies on peptide loading of rat class II and on peptide persistence in cells of the dendritic lineage prompted us to establish full length cDNA clones coding for the subunits alpha and beta of rat DM molecules as well as a mAb directed against the luminal moiety of the beta subunit. Here we describe the establishment of the first full length cDNA clones of rat RT1.DMa and RT1.DMb. The mode of expression of RT1.DM at the transcript level in bone marrow culture-derived dendritic cells, in Langerhans cells and in a number of additional accessory cells is reported. The beta protein was identified in detergent lysates of RT1.DM expressing cells by Western blot analysis using a newly established monoclonal antibody directed against the luminal part of RT1.DMbeta.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Kurth D,Neumann J,Demleitner K,Hildmann J,Mehlig M,Scheicher C,Wendling U,Sun D,Reske Kdoi
10.1515/bchm.1997.378.9.1005subject
Has Abstractpub_date
1997-09-01 00:00:00pages
1005-12issue
9eissn
1431-6730issn
1437-4315journal_volume
378pub_type
杂志文章abstract::Full-length Hsp70 protein (Hsp70) and the C-terminal domain of Hsp70 (Hsp70C) both stimulate the cytolytic activity of naive natural killer (NK) cells against Hsp70-positive tumor target cells. Here, we describe the characterization of Hsp70-NK cell interaction with binding studies using the human NK cell line YT. Bin...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.030
更新日期:2003-02-01 00:00:00
abstract::Arginine deiminase (ADI) is a potential antitumor agent for the arginine deprivation treatment of L-arginine auxotrophic tumors. The optimum pH of ADI varies significantly, yet little is known about the origin of this variety. Here, Pseudomonas aeruginosa ADI (PaADI), an enzyme that functions only at acidic pH, was ut...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2012-0183
更新日期:2012-09-01 00:00:00
abstract::We have previously described Kunitz-type serine proteinase inhibitors purified from Bauhinia seeds. Human plasma kallikrein shows different susceptibility to those inhibitors. In this communication, we describe the interaction of human plasma kallikrein with fluorogenic and non-fluorogenic peptides based on the Bauhin...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.016
更新日期:2001-01-01 00:00:00
abstract::Selective adhesion of fungal cells to one another and to foreign surfaces is fundamental for the development of multicellular growth forms and the successful colonization of substrates and host organisms. Accordingly, fungi possess diverse cell wall-associated adhesins, mostly large glycoproteins, which present N-term...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2020-0199
更新日期:2020-11-26 00:00:00
abstract::In this work we demonstrate that heat and pressure induce only slightly different energetic changes in the unfolded state of RNase A. Using pressure and temperature as denaturants on a significant number of variants, and by determining the free energy of unfolding at different temperatures, we estimated the stability ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.038
更新日期:2006-03-01 00:00:00
abstract::Alternative splicing is a process by which multiple messenger RNAs (mRNAs) are generated from a single pre-mRNA, resulting in functionally distinct protein products. This is accomplished by the differential recognition of splice sites in the pre-mRNA, often regulated in a tissue- or development-specific manner. Altern...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2005.143
更新日期:2005-12-01 00:00:00
abstract::The expression of the cysteine protease cathepsin B is increased in early stages of human breast cancer.To assess the potential role of cathepsin B in premalignant progression of breast epithelial cells, we employed a 3D reconstituted basement membrane overlay culture model of MCF10A human breast epithelial cells and ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2012-0252
更新日期:2012-12-01 00:00:00
abstract::Signalling molecules are produced, degraded, modified and transported throughout the development of higher organisms. Understanding their mode of action implies understanding these dynamics in vivo and in real time. Genetically encoded biosensors are being more and more used as tools to 'follow' signalling molecules a...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0310
更新日期:2019-03-26 00:00:00
abstract::The enzymatic properties of glycosylphosphatidylinositol-specific phospholipase D (EC 3.1.4.50) were characterized using a 6,000-fold purified enzyme. This was obtained in 100 microg amounts from human serum with a recovery of 35%. Pure alkaline phosphatase containing one anchor moiety per molecule was used as substra...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.062
更新日期:2000-05-01 00:00:00
abstract::Synthetic biologists aim at engineering controllable biological parts such as DNA, RNA and proteins in order to steer biological activities using external inputs. Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-t...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0309
更新日期:2019-03-26 00:00:00
abstract::Haptoglobin (Hpt) is known to capture circulating free hemoglobin (Hb) and bind apolipoprotein (Apo) A-I or E. Here, we report that Hb can be tightly bound by most of Hpt molecules (TB-Hpt, 80%), whereas loosely bound by a minor part of them (LB-Hpt, 20%). LB-Hpt amount was significantly increased (over 60%) in patien...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2011.033
更新日期:2011-04-01 00:00:00
abstract::The three isoforms of human Ins(1,4,5)P3 3-kinase (IP3K) show remarkable differences in their intracellular targeting. Whereas predominant targeting to the cytoskeleton and endoplasmic reticulum has been shown for IP3K-A and IP3K-B, rat IP3K-C shuttles actively between the nucleus and cytoplasm. In the present study w...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.075
更新日期:2006-05-01 00:00:00
abstract::In pancreatic acinar cells stimulation of different intracellular pathways leads to different patterns of Ca2+ signaling. Bombesin induces activation of both phosphatidylinositol 4,5-bisphosphate (PIP2)-specific phospholipase C (PLC) and phospholipase D (PLD). The latter leads to generation of diacylglycerol (DAG) in ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.1999.111
更新日期:1999-07-01 00:00:00
abstract::Mitochondria are indispensable cellular organelles providing ATP and numerous other essential metabolites to ensure cell survival. Reactive oxygen species (ROS), which are formed as side reactions during oxidative phosphorylation or by external agents, induce molecular damage in mitochondrial proteins, lipids/membrane...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2017-0206
更新日期:2017-12-20 00:00:00
abstract::Proteomic identification of protease cleavage site specificity (PICS) is a recent proteomic approach for the easy mapping of protease subsite preferences that determines both the prime- and non-prime side specificity concurrently. Here we greatly facilitate user access by providing an automated and simple web-based da...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2011.158
更新日期:2011-11-01 00:00:00
abstract::The activities of cathepsin B, L, J and H in rat liver were significantly increased by starvation if compared with normal diet rats. Furthermore, the activity of cathepsin L increased with glucagon treatment, and the activities of cathepsin L and H decreased significantly with insulin treatment. The changes in catheps...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract::The lysosomal cysteine proteases cathepsin B and L play important roles in tumor cell invasion. An imbalance between these cathepsins and their endogenous inhibitors, the cystatins, has been associated with development of the metastatic phenotype. Accordingly, many studies have indicated potential use of cystatins in ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.035
更新日期:2008-04-01 00:00:00
abstract::Many neurodegenerative disorders and syndromes are associated with an excessive generation of reactive oxygen species (ROS) and oxidative stress. The pathways to nerve cell death induced by diverse potential neurotoxins such as peptides, excitatory amino acids, cytokines or synthetic drugs commonly share oxidative dow...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2002.053
更新日期:2002-03-01 00:00:00
abstract::The non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase catalyses the irreversible reaction of glyceraldehyde-3-phosphate to 3-phosphoglycerate by the reduction of NADP to NADPH. This is in contrast to the extensively analysed phosphorylating glyceraldehyde-3-phosphate dehydrogenases which catalyse the revers...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1997-12-01 00:00:00
abstract::Fanconi anemia is a fatal, hereditary chromosome instability syndrome of early childhood with progressive pancytopenia and cancer-proneness. Hypersensitivity to alkylating agents points to DNA repair inefficiency. Excess reactive oxygen intermediates and hypersensitivity to oxygen, all features of Fanconi anemia cells...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.151
更新日期:2008-10-01 00:00:00
abstract::Mitochondria contain elaborate machineries for the import of precursor proteins from the cytosol. The translocase of the outer mitochondrial membrane (TOM) performs the initial import of precursor proteins and transfers the precursors to downstream translocases, including the presequence translocase and the carrier tr...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2016-0145
更新日期:2016-11-01 00:00:00
abstract::The primary cilium is a sensory organelle that is vital in regulating several signalling pathways. Unlike most organelles cilia are open to the rest of the cell, not enclosed by membranes. The distinct protein composition is crucial to the function of cilia and many signalling proteins and receptors are specifically c...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2017-0168
更新日期:2017-12-20 00:00:00
abstract::To elucidate the structures and dynamics of membrane proteins, highly advanced biophysical methods have been developed that often require significant resources, both for sample preparation and experimental analyses. For very complex systems, such as membrane transporters, ion channels or G-protein coupled receptors (G...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0473
更新日期:2019-09-25 00:00:00
abstract::Kallikrein 6 (KLK6) is a trypsin-like serine peptidase whose relevance in various types of cancers is currently being explored. Previous studies have shown that KLK6 mRNA is upregulated in colon and gastric cancers; however, the regulatory mechanisms and phenotypic consequences of this upregulation are largely unknown...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.087
更新日期:2008-06-01 00:00:00
abstract::The neurobiological mechanisms of obesity-induced peripheral neuropathy are poorly understood. We evaluated the role of Sigma-1 receptor (Sig-1R) and NMDA receptor (NMDARs) in the spinal cord in peripheral neuropathy using an animal model of high fat diet-induced diabetes. We examined the expression of Sig-1R and NMDA...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2017-0117
更新日期:2017-09-26 00:00:00
abstract::The cloning of a Ca2+(0)-sensing receptor (CaR) from parathyroid and kidney, coupled with the identification of inherited disorders in Ca2+(0)-sensing resulting from inactivating or activating mutations of this CaR, has provided substantial insights into how Ca2+(0) is regulated at a systemic level. The CaR plays a ce...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1997-08-01 00:00:00
abstract::We present a novel strategy, termed CISTEM, which allows direct in vivo screening of polypeptides displayed on the surface of E. coli cells by a combination of ligand-mediated protection and phage-mediated selection. The effectiveness of this new approach was demonstrated by displaying the T7.tag on the surface of E. ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.202
更新日期:2001-12-01 00:00:00
abstract::This review addresses many of the chemical aspects of nitrosative stress mediated by N2O3. From a cellular perspective, N2O3 and the resulting reactive nitrogen oxide species target specific motifs such as thiols, lysine active sites, and zinc fingers and is dependant upon both the rates of production as well as consu...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2004.001
更新日期:2004-01-01 00:00:00
abstract::Matrix metalloproteinases (MMPs) are a family of zinc endopeptidases that have been implicated in various disease processes. Different classes of MMP inhibitors, including hydroxamic acids, phosphinic acids and thiols, have been previously described. Most of these mimic peptides and most likely bind in a similar way t...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.159
更新日期:2001-08-01 00:00:00
abstract::Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes. ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.062
更新日期:2001-03-01 00:00:00