Pressure- and temperature-induced unfolding studies: thermodynamics of core hydrophobicity and packing of ribonuclease A.

abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV), or human herpesvirus 8 (HHV8) is a gammaherpesvirus and the etiological agent of Kaposi's sarcoma, primary effusion lymphoma and multicentric Castleman disease. The KSHV genome contains genes for a unique group of proteins with homology to cellular interferon regulatory ...

journal_title：Biological chemistry

authors： Koch S,Schulz TF

更新日期：2017-07-26 00:00:00

abstract：:Characterization of protein damage during photosensitization of chlorin e6-treated cells was performed using the green fluorescent protein (GFP). The GFP-chromophore damage caused by singlet oxygen was studied in COS 7 kidney cells and E. coli bacteria following light irradiation. Electron spin resonance (ESR) reveale...

journal_title：Biological chemistry

authors： Greenbaum L,Rothmann C,Lavie R,Malik Z

更新日期：2000-12-01 00:00:00

abstract：:The activities of cathepsin B, L, J and H in rat liver were significantly increased by starvation if compared with normal diet rats. Furthermore, the activity of cathepsin L increased with glucagon treatment, and the activities of cathepsin L and H decreased significantly with insulin treatment. The changes in catheps...

journal_title：Biological chemistry

authors： Inubushi T,Shikiji M,Endo K,Kakegawa H,Kishino Y,Katunuma N

更新日期：1996-07-01 00:00:00

abstract：:Mitochondria are indispensable cellular organelles providing ATP and numerous other essential metabolites to ensure cell survival. Reactive oxygen species (ROS), which are formed as side reactions during oxidative phosphorylation or by external agents, induce molecular damage in mitochondrial proteins, lipids/membrane...

journal_title：Biological chemistry

authors： Zimmermann M,Reichert AS

更新日期：2017-12-20 00:00:00

abstract：:Among the Ras family, Ran is a unique small G protein. It does not have a lipid modification motif at the C-terminus to bind to the membrane, which is often observed within the Ras family. Ran may therefore interact with a wide range of proteins in various intracellular locations. This means that Ran could play many d...

journal_title：Biological chemistry

authors： Nishimoto T

更新日期：2000-05-01 00:00:00

abstract：:The influence of the cellular milieu, a complex and crowded solvent, is often neglected when biomolecular structure and function are studied in vitro. To mimic the cellular environment, crowding effects are commonly induced in vitro using artificial crowding agents like Ficoll or dextran. However, it is unclear if suc...

journal_title：Biological chemistry

authors： Gnutt D,Ebbinghaus S

更新日期：2016-01-01 00:00:00

abstract：:The production and processing of ribosomal RNA is a complex and well-coordinated nucleolar process for ribosome biogenesis. Progress in understanding nucleolar structure and function has lead to the unexpected discovery of the nucleolus as a highly sensitive sensor of cellular stress and an important regulator of the ...

journal_title：Biological chemistry

authors： Burger K,Eick D

更新日期：2013-09-01 00:00:00

abstract：:Isolated human granulocyte plasma membranes contain progelatinase B. The binding of progelatinase B to the membrane, however, is relatively weak, and a considerable part of progelatinase B can be removed by simply washing the membrane with buffer. This detachment does not depend on the ionic strength of the buffer, in...

journal_title：Biological chemistry

authors： Kolkenbrock H,Zimmermann J,Burmester GR,Ulbrich N

更新日期：2000-01-01 00:00:00

abstract：:Biofilms are a ubiquitous mode of microbial life and display an increased tolerance to different stresses. Inside biofilms, cells may experience both externally applied stresses and internal stresses that emerge as a result of growth in spatially structured communities. In this review, we discuss the spatial scales of...

journal_title：Biological chemistry

authors： Rode DKH,Singh PK,Drescher K

更新日期：2020-11-26 00:00:00

abstract：:A controlled interaction of materials with their surrounding biological environment is of great interest in many fields. Multifunctional coatings aim to provide simultaneous modulation of several biological signals. They can consist of various combinations of bioactive, and bioinert components as well as of reporter m...

journal_title：Biological chemistry

authors： Pagel M,Beck-Sickinger AG

更新日期：2017-01-01 00:00:00

abstract：:The physiological roles of the intracellular iron and redox regulatory systems are intimately linked. Iron is an essential trace element for most organisms, yet elevated cellular iron levels are a potent generator and amplifier of reactive oxygen species and redox stress. Proteins binding iron or iron-sulfur (Fe/S) cl...

journal_title：Biological chemistry

authors： Mühlenhoff U,Braymer JJ,Christ S,Rietzschel N,Uzarska MA,Weiler BD,Lill R

更新日期：2020-11-26 00:00:00

abstract：:A novel antifungal protein, designated chrysancorin, was isolated from seeds of Chrysanthemum coronarium var. spatiosum with a procedure involving ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue resin, ion exchange chromatography on SP-Sepharose and FPLC-gel filtration on Superd...

journal_title：Biological chemistry

authors： Wang H,Ye XY,Ng TB

更新日期：2001-06-01 00:00:00

abstract：:DEAD-box helicases catalyze RNA duplex unwinding in an ATP-dependent reaction. Members of the DEAD-box helicase family consist of a common helicase core formed by two RecA-like domains. According to the current mechanistic model for DEAD-box mediated RNA unwinding, binding of RNA and ATP triggers a conformational chan...

journal_title：Biological chemistry

authors： Rudolph MG,Klostermeier D

更新日期：2015-08-01 00:00:00

abstract：:SlyD is a bacterial two-domain protein that functions as a molecular chaperone, a prolyl cis/trans isomerase, and a nickel-binding protein. This review summarizes recent findings about the molecular enzyme mechanism of SlyD. The chaperone function located in one domain of SlyD is involved in twin-arginine translocatio...

journal_title：Biological chemistry

authors： Kovermann M,Schmid FX,Balbach J

更新日期：2013-08-01 00:00:00

abstract：:The hair follicle consists of a complex system of multiple tissue compartments that are clearly distinguishable by their morphology and type of differentiation. We have synthesized hair follicle-specific keratins from the companion layer (K6hf, K17) and the hair cortex (Ha1, Hb3, Hb6) in Escherichia coli. The assembly...

journal_title：Biological chemistry

authors： Hofmann I,Winter H,Mücke N,Langowski J,Schweizer J

更新日期：2002-09-01 00:00:00

abstract：:In a recent study we showed that two proteinases (CMS2MS2 and CMS2MS3) from Carica candamarcensis enhance mammalian cell proliferation. The aim of the present study is the determination of the primary structure of CMS2MS2 and prediction of its three-dimensional structure. The protein contains 214 residues, including t...

journal_title：Biological chemistry

authors： Gomes MT,Bemquerer MP,Lopes MT,Richardson M,Júnior SO,Salas CE

更新日期：2007-08-01 00:00:00

abstract：:The lysosomal lumen contains numerous acidic hydrolases involved in the degradation of carbohydrates, lipids, proteins, and nucleic acids, which are basic cell components that turn over continuously within the cell and/or are ingested from outside of the cell. Deficiency in almost any of these hydrolases causes accumu...

journal_title：Biological chemistry

authors： Golabek AA,Kida E

更新日期：2006-08-01 00:00:00

abstract：:Loss of genetic material from chromosome 3p21.3 is one of the most common and earliest events in the pathogenesis of lung cancer and many other solid tumors. The chromosomal area 3p21.3 is thought to harbor at least one important tumor suppressor gene, which, despite many years of investigation, has remained elusive. ...

journal_title：Biological chemistry

authors： Pfeifer GP,Yoon JH,Liu L,Tommasi S,Wilczynski SP,Dammann R

更新日期：2002-06-01 00:00:00

abstract：:The cysteine-type peptidase cathepsin X is highly upregulated in several cancers and presumably promotes tumor invasion through bypassing cellular senescence. Here, we present first evidence that the underlying mechanism may involve the regulation of the insulin-like growth factor (IGF) system, a well-known activator ...

journal_title：Biological chemistry

authors： Kraus S,Fruth M,Bunsen T,Nägler DK

更新日期：2012-12-01 00:00:00

abstract：:The kallikrein-related peptidase (KLK) gene family of 15 serine proteases encodes many proteins, including prostate specific antigen (PSA or KLK3), that are well described and/or are potential biomarkers for hormone-related cancers. Variant mRNA transcripts produced by alternative splicing, polyadenylation or AUG site...

journal_title：Biological chemistry

authors： Tan OL,Whitbread AK,Clements JA,Dong Y

更新日期：2006-06-01 00:00:00

abstract：:Based on studies presented here and other published experiments performed with surviving tissue preparations, with transfected cells and with cells that constitutively express the human angiotensin I converting enzyme ACE and B2 receptors, we concluded the following: ACE inhibitors and other endogenous peptides that r...

journal_title：Biological chemistry

authors： Erdös EG,Marcic BM

更新日期：2001-01-01 00:00:00

abstract：:Matrix metalloproteinases (MMPs) are a family of zinc endopeptidases that have been implicated in various disease processes. Different classes of MMP inhibitors, including hydroxamic acids, phosphinic acids and thiols, have been previously described. Most of these mimic peptides and most likely bind in a similar way t...

journal_title：Biological chemistry

authors： Grams F,Brandstetter H,D'Alò S,Geppert D,Krell HW,Leinert H,Livi V,Menta E,Oliva A,Zimmermann G,Gram F,Brandstetter H,D'Alò S,Geppert D,Krell HW,Leinert H,Livi VMenta E,Oliva A,Zimmermann G

更新日期：2001-08-01 00:00:00

abstract：:Abstract Fanconi anemia (FA) is a rare cancer-prone genetic disorder characterized by progressive bone marrow failure, chromosomal instability and redox abnormalities. There is much biochemical and genetic data, which strongly suggest that FA cells experience increased oxidative stress. The present study was designed ...

journal_title：Biological chemistry

authors： Petrovic S,Leskovac A,Kotur-Stevuljevic J,Joksic J,Guc-Scekic M,Vujic D,Joksic G

更新日期：2011-07-01 00:00:00

abstract：:Dipeptidyl peptidase-IV has been demonstrated to play a role in cancer biology by many authors. Since then, additional proteins possessing similar enzymatic activity have been described and their role in cancerogenesis has been hypothesized. To assess the complexity of these 'Dipeptidyl peptidase-IV activity and/or st...

journal_title：Biological chemistry

authors： Sedo A,Busek P,Scholzová E,Malík R,Vlasicová K,Janácková S,Mares V

更新日期：2004-06-01 00:00:00

abstract：:As an important protein, UL31 has been demonstrated to play multiple roles in herpes simplex virus 1 (HSV-1) replication. Previous studies showed that UL31 predominantly locates in the nucleus in chemical fixed cells and live cells, however, the determining mechanisms for its nuclear translocation is not clear. In the...

journal_title：Biological chemistry

authors： Cai M,Si J,Li X,Zeng Z,Li M

更新日期：2016-06-01 00:00:00

abstract：:The main function of the epidermis is to establish a vital multifunctional barrier between the body and its external environment. A defective epidermal barrier is one of the key features of atopic dermatitis (AD), a chronic and relapsing inflammatory skin disorder that affects up to 20% of children and 2-3% of adults ...

journal_title：Biological chemistry

authors： Le Lamer M,Pellerin L,Reynier M,Cau L,Pendaries V,Leprince C,Méchin MC,Serre G,Paul C,Simon M

更新日期：2015-11-01 00:00:00

abstract：:The heterodimer consisting of ecdysteroid receptor (EcR) and ultraspiracle (USP), both of which are members of the nuclear receptor superfamily, is considered to be the functional ecdysteroid receptor. Here we analyzed the subcellular distribution of EcR and USP fused to fluorescent proteins. The experiments were carr...

journal_title：Biological chemistry

authors： Nieva C,Gwóźdź T,Dutko-Gwóźdź J,Wiedenmann J,Spindler-Barth M,Wieczorek E,Dobrucki J,Duś D,Henrich V,Ozyhar A,Spindler KD

更新日期：2005-05-01 00:00:00

abstract：:Metachromatic leukodystrophy is a lysosomal disorder caused by the deficiency of arylsulfatase A (ASA). This leads to the storage of the sphingolipid 3-O-sulfogalactosylceramide (sulfatide) in various cell types, such as renal tubular cells. Examination of mannose 6-phosphate receptor (MPR300)-dependent endocytosis re...

journal_title：Biological chemistry

authors： Klein D,Yaghootfam A,Matzner U,Koch B,Braulke T,Gieselmann V

更新日期：2009-01-01 00:00:00

abstract：:Cysteine cathepsins are expressed in most tissues, including the gastrointestinal tract. We demonstrated an involvement of mouse intestinal cathepsin B in extracellular matrix remodeling for regeneration from trauma. The present study aimed at elucidating roles of cysteine cathepsins in the non-traumatized gastrointes...

journal_title：Biological chemistry

authors： Tamhane T,Arampatzidou M,Gerganova V,Tacke M,Illukkumbura R,Dauth S,Schaschke N,Peters C,Reinheckel T,Brix K

更新日期：2014-10-01 00:00:00

abstract：:The signal recognition particle (SRP), a ubiquitous cytoplasmic ribonucleoprotein particle, plays an essential role in promoting co-translational translocation of proteins into the endoplasmic reticulum. Here, we summarise recent progress made in the understanding of two essential SRP functions: the signal recognition...

journal_title：Biological chemistry

authors： Bui N,Strub K

更新日期：1999-02-01 00:00:00