Expression of human pro-matrix metalloproteinase 3 that lacks the N-terminal 34 residues in Escherichia coli: autoactivation and interaction with tissue inhibitor of metalloproteinase 1 (TIMP-1).

Abstract:

:Human pro-matrix metalloproteinase 3 (proMMP-3) lacking the N-terminal 34 amino acids and the C-terminal hemopexin-like domain was expressed in E. coli and used to investigate the process of proenzyme activation and its interaction with an endogenous inhibitor TIMP-1 during activation. The truncated precursor was purified from the E. coli extract in the presence of 5mM EGTA. The active 23.5 kDa form was generated simply by exposure to Ca2+ and Zn2+ but not either by Ca2+ alone or by Zn2+ alone. The rate of MMP-3(deltaC) formation was concentration dependent, indicating that autoactivation is a bimolecular reaction. The truncated precursor was able to interact with the N-terminal domain of TIMP-1 without losing the 48 residue-long propeptide. However, upon a longer incubation, the propeptide was slowly processed, indicating that the association of the N-terminally truncated proMMP-3 with TIMP-1 is weaker than that of the fully activated MMP-3 and TIMP-1. These results indicate that the expression of MMP activities is regulated by endogenous inhibitor TIMPs during their activation processes which provide an additional control mechanism of extracellular matrix breakdown.

journal_name

Biol Chem

journal_title

Biological chemistry

authors

Suzuki K,Kan CC,Hung W,Gehring MR,Brew K,Nagase H

doi

10.1515/bchm.1998.379.2.185

subject

Has Abstract

pub_date

1998-02-01 00:00:00

pages

185-91

issue

2

eissn

1431-6730

issn

1437-4315

journal_volume

379

pub_type

杂志文章
  • Identification of plasma haptoglobin forms which loosely bind hemoglobin.

    abstract::Haptoglobin (Hpt) is known to capture circulating free hemoglobin (Hb) and bind apolipoprotein (Apo) A-I or E. Here, we report that Hb can be tightly bound by most of Hpt molecules (TB-Hpt, 80%), whereas loosely bound by a minor part of them (LB-Hpt, 20%). LB-Hpt amount was significantly increased (over 60%) in patien...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2011.033

    authors: Spagnuolo MS,Cigliano L,Maresca B,Pugliese CR,Abrescia P

    更新日期:2011-04-01 00:00:00

  • Reverse action of ribonuclease T1 in frozen aqueous systems.

    abstract::We have studied ribonuclease T1 (EC 3.1-27.3)-catalysed synthesis of guanylyl-(3'-->5')cytidine in frozen aqueous reaction mixtures at -10 degrees C and in solution at 0 degree C in order to investigate whether ribonuclease-catalysed synthetic reactions can take advantage of the yield-increasing effect of freezing as ...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/bchm.1997.378.2.115

    authors: Haensler M,Hahn U,Jakubke HD

    更新日期:1997-02-01 00:00:00

  • Kallikrein 6 is a mediator of K-RAS-dependent migration of colon carcinoma cells.

    abstract::Kallikrein 6 (KLK6) is a trypsin-like serine peptidase whose relevance in various types of cancers is currently being explored. Previous studies have shown that KLK6 mRNA is upregulated in colon and gastric cancers; however, the regulatory mechanisms and phenotypic consequences of this upregulation are largely unknown...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2008.087

    authors: Henkhaus RS,Gerner EW,Ignatenko NA

    更新日期:2008-06-01 00:00:00

  • Novel approach to quorum quenching: rational design of antibacterials in combination with hexahistidine-tagged organophosphorus hydrolase.

    abstract::N-acyl homoserine lactones (AHLs) are quorum sensing (QS) signal molecules used by most Gram-negative pathogenic bacteria. In this article the lactonase activity of the preparations based on hexahistidine-tagged organophosphorus hydrolase (His6-OPH) towards AHLs was studied. Initially, three of the most interesting β-...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/hsz-2018-0162

    authors: Aslanli A,Lyagin I,Efremenko E

    更新日期:2018-07-26 00:00:00

  • Efficacy of HOCl scavenging by sulfur-containing compounds: antioxidant activity of glutathione disulfide?

    abstract::Hypochlorous acid (HOCl) is a bactericidal compound formed by activated neutrophils during inflammation. Overproduction of HOCl causes damage to tissues at the site of neutrophil accumulation. The deleterious effects of excessive HOCl formation can be attenuated using antioxidants. Thiols and thioethers are known to b...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2002.073

    authors: den Hartog GJ,Haenen GR,Vegt E,van der Vijgh WJ,Bast A

    更新日期:2002-03-01 00:00:00

  • Activation of GPR119 by fatty acid agonists augments insulin release from clonal β-cells and isolated pancreatic islets and improves glucose tolerance in mice.

    abstract::G-protein coupled receptor 119 (GPR119) is emerging as a potential target for the treatment of type 2 diabetes with beneficial effects on glucose homeostasis. This study assessed the insulin-secreting properties of various GPR119 agonists and the distribution of GPR119 in pancreatic islets. Endogenous ligands [oleoyle...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/hsz-2013-0255

    authors: Moran BM,Abdel-Wahab YH,Flatt PR,McKillop AM

    更新日期:2014-04-01 00:00:00

  • Activation of progelatinase A by mammalian legumain, a recently discovered cysteine proteinase.

    abstract::The activation of progelatinase A to gelatinase A requires cleavage of an asparaginyl bond to form the N-terminus of the mature enzyme. We have asked whether the activation can be mediated by legumain, the recently discovered lysosomal cysteine proteinase that is specific for hydrolysis of asparaginyl bonds. Addition ...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2001.093

    authors: Chen JM,Fortunato M,Stevens RA,Barrett AJ

    更新日期:2001-05-01 00:00:00

  • Purification and MALDI-MS characterization of stressin, a stress-associated glycoprotein.

    abstract::Glycoconjugates have a whole spectrum of biological roles, from those that appear trivial to those that are crucial. Results accumulated in the past years indicate they might also play an important role in the response to stress, a complex physiological response of the human organism to various threats. We have recent...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.1999.058

    authors: Lauc G,Peter-Katalinić J,Dabelić S,Flögel M

    更新日期:1999-04-01 00:00:00

  • Diversity of GPI-anchored fungal adhesins.

    abstract::Selective adhesion of fungal cells to one another and to foreign surfaces is fundamental for the development of multicellular growth forms and the successful colonization of substrates and host organisms. Accordingly, fungi possess diverse cell wall-associated adhesins, mostly large glycoproteins, which present N-term...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审

    doi:10.1515/hsz-2020-0199

    authors: Essen LO,Vogt MS,Mösch HU

    更新日期:2020-11-26 00:00:00

  • S1PR4 is required for plasmacytoid dendritic cell differentiation.

    abstract::The sphingolipid sphingosine-1-phosphate (S1P) has various functions in immune cell biology, regulating survival, proliferation, and, most prominently, migration. S1P couples to five G protein-coupled receptors (S1PR1-5) to transduce its effects on immune cell function. Expression of S1PR4 is restricted to immune cell...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/hsz-2014-0271

    authors: Dillmann C,Mora J,Olesch C,Brüne B,Weigert A

    更新日期:2015-06-01 00:00:00

  • Purification and cloning of type A/B hnRNP proteins involved in transcriptional activation from the Rat spi 2 gene GAGA box.

    abstract::The GAGA box of the rat serine protease inhibitor 2 (spi 2) genes not only acts as a basal promoter element, but also mediates transcriptional activation by growth hormone and interleukin-6. The GAGA box is separated from the TATA box by only 12 bp, and this close association is required for efficient transcription. H...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2000.127

    authors: Leverrier S,Cinato E,Paul C,Derancourt J,Bemark M,Leanderson T,Legraverend C

    更新日期:2000-11-01 00:00:00

  • Tropospheric ozone: an emerging environmental stress to skin.

    abstract::As the outermost barrier of the body, the skin is directly and frequently exposed to a prooxidative environment, including solar UVA and UVB radiation, and air pollution. Ozone, a major air pollutant, possesses a strong oxidizing potential and therefore is possibly one of the most reactive chemicals the skin ever meet...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审

    doi:10.1515/bchm.1997.378.11.1299

    authors: Thiele JJ,Podda M,Packer L

    更新日期:1997-11-01 00:00:00

  • Pressure- and temperature-induced unfolding studies: thermodynamics of core hydrophobicity and packing of ribonuclease A.

    abstract::In this work we demonstrate that heat and pressure induce only slightly different energetic changes in the unfolded state of RNase A. Using pressure and temperature as denaturants on a significant number of variants, and by determining the free energy of unfolding at different temperatures, we estimated the stability ...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2006.038

    authors: Font J,Benito A,Torrent J,Lange R,Ribó M,Vilanova M

    更新日期:2006-03-01 00:00:00

  • Cellular expression of plasma prekallikrein in human tissues.

    abstract::Plasma prekallikrein (PPK) is synthesised in hepatocytes and secreted into the blood, where it participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. Recently we demonstrated by quantitative RT-PCR that the human PPK gene is transcribed not only in the l...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2007.104

    authors: Fink E,Bhoola KD,Snyman C,Neth P,Figueroa CD

    更新日期:2007-09-01 00:00:00

  • GAPDH enhances group II intron splicing in vitro.

    abstract::Group II introns are autocatalytic RNAs which self-splice in vitro. However, in vivo additional protein factors might be involved in the splicing process. We used an affinity chromatography method called 'StreptoTag' to identify group II intron binding proteins from Saccharomyces cerevisiae. This method uses a hybrid ...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2004.076

    authors: Böck-Taferner P,Wank H

    更新日期:2004-07-01 00:00:00

  • Hormonal and dietary regulation of lysosomal cysteine proteinases in liver under gluconeogenesis conditions.

    abstract::The activities of cathepsin B, L, J and H in rat liver were significantly increased by starvation if compared with normal diet rats. Furthermore, the activity of cathepsin L increased with glucagon treatment, and the activities of cathepsin L and H decreased significantly with insulin treatment. The changes in catheps...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:

    authors: Inubushi T,Shikiji M,Endo K,Kakegawa H,Kishino Y,Katunuma N

    更新日期:1996-07-01 00:00:00

  • The role of protein phosphatase 2A catalytic subunit Calpha in embryogenesis: evidence from sequence analysis and localization studies.

    abstract::Protein phosphatase 2A (PP2A) constitutes one of the major families of protein serine/threonine phosphatases found in all eukaryotic cells. PP2A holoenzymes are composed of a catalytic subunit complexed with a structural regulatory subunit of 65 kDa. These core subunits associate with regulatory subunits of various si...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.1999.139

    authors: Götz J,Kues W

    更新日期:1999-09-01 00:00:00

  • Assembly and function of small RNA - argonaute protein complexes.

    abstract::Small RNAs such as microRNAs (miRNAs), short interfering RNAs (siRNAs) or Piwi-interacting RNAs (piRNAs) are important regulators of gene expression in various organisms. Small RNAs bind to a member of the Argonaute protein family and are incorporated into larger structures that mediate diverse gene silencing events. ...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审

    doi:10.1515/hsz-2014-0116

    authors: Dueck A,Meister G

    更新日期:2014-06-01 00:00:00

  • Correlating structure and ligand affinity in drug discovery: a cautionary tale involving second shell residues.

    abstract::A high-resolution crystallographic structure determination of a protein-ligand complex is generally accepted as the 'gold standard' for structure-based drug design, yet the relationship between structure and affinity is neither obvious nor straightforward. Here we analyze the interactions of a series of serine protein...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/hsz-2014-0158

    authors: Tziridis A,Rauh D,Neumann P,Kolenko P,Menzel A,Bräuer U,Ursel C,Steinmetzer P,Stürzebecher J,Schweinitz A,Steinmetzer T,Stubbs MT

    更新日期:2014-07-01 00:00:00

  • Processive proteolysis by γ-secretase and the mechanism of Alzheimer's disease.

    abstract::γ-Secretase is a membrane-embedded protease complex with presenilin as the catalytic component. Cleavage within the transmembrane domain of the amyloid β-protein precursor (APP) by γ-secretase produces the C-terminus of the amyloid β-peptide (Aβ), a proteolytic product prone to aggregation and strongly linked to Alzhe...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审

    doi:10.1515/hsz-2012-0140

    authors: Wolfe MS

    更新日期:2012-09-01 00:00:00

  • Interaction of heat shock protein 70 peptide with NK cells involves the NK receptor CD94.

    abstract::Full-length Hsp70 protein (Hsp70) and the C-terminal domain of Hsp70 (Hsp70C) both stimulate the cytolytic activity of naive natural killer (NK) cells against Hsp70-positive tumor target cells. Here, we describe the characterization of Hsp70-NK cell interaction with binding studies using the human NK cell line YT. Bin...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2003.030

    authors: Gross C,Hansch D,Gastpar R,Multhoff G

    更新日期:2003-02-01 00:00:00

  • Traces of post-transcriptional RNA modifications in deep sequencing data.

    abstract::Many aspects of the RNA maturation leave traces in RNA sequencing data in the form of deviations from the reference genomic DNA. This includes, in particular, genomically non-encoded nucleotides and chemical modifications. The latter leave their signatures in the form of mismatches and conspicuous patterns of sequenci...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2011.043

    authors: Findeiss S,Langenberger D,Stadler PF,Hoffmann S

    更新日期:2011-04-01 00:00:00

  • Effects of reducing agents on glutathione metabolism and the function of carotid body chemoreceptor cells.

    abstract::Two current hypotheses of O2 sensing in the carotid body (CB) chemoreceptors suggest participation of oxygen reactive (ROS) species, but they are mechanistically opposed. One postulates that hypoxia decreases ROS levels; the other that hypoxia increases them. Yet, both propose that the ensuing alteration in the cellul...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2004.021

    authors: Gonzalez C,Sanz-Alyayate G,Agapito MT,Obeso A

    更新日期:2004-03-01 00:00:00

  • Specific inhibition of interleukin-13 activity by a recombinant human single-chain immunoglobulin domain directed against the IL-13 receptor alpha1 chain.

    abstract::Interleukin-13 (IL-13) is a T-cell-derived pleiotropic cytokine of particular medical importance because of its critical role in the development of allergic asthma. The effects of IL-13 on its target cells are mediated through a dimeric transmembrane receptor (IL-13R), which shares the IL-4Ralpha subunit with the IL-4...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2007.036

    authors: Knackmuss S,Krause S,Engel K,Reusch U,Virchow JC,Mueller T,Kraich M,Little M,Luttmann W,Friedrich K

    更新日期:2007-03-01 00:00:00

  • The activity and localization patterns of cathepsins B and X in cells of the mouse gastrointestinal tract differ along its length.

    abstract::Cysteine cathepsins are expressed in most tissues, including the gastrointestinal tract. We demonstrated an involvement of mouse intestinal cathepsin B in extracellular matrix remodeling for regeneration from trauma. The present study aimed at elucidating roles of cysteine cathepsins in the non-traumatized gastrointes...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/hsz-2014-0151

    authors: Tamhane T,Arampatzidou M,Gerganova V,Tacke M,Illukkumbura R,Dauth S,Schaschke N,Peters C,Reinheckel T,Brix K

    更新日期:2014-10-01 00:00:00

  • Thyroid hormone receptors bind to an element in the connexin43 promoter.

    abstract::Thyroid hormones stimulate gap junctional communication in rat liver WB-F344 epithelial cells, elevating connexin43 mRNA and protein levels. In the present work we analysed connexin43 expression in liver and heart samples from thyroid hormone-treated Wistar rats. Connexin43 mRNA was elevated 2.1-fold in rat liver samp...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2000.120

    authors: Stock A,Sies H

    更新日期:2000-09-01 00:00:00

  • Analysis of the autoproteolytic activity of the recombinant helper component proteinase from zucchini yellow mosaic virus.

    abstract::The multifunctional helper component proteinase (HC-Pro) of potyviruses contains an autoproteolytic function that, together with the protein 1 (P1) and NIa proteinase, processes the polyprotein into mature proteins. In this study, we analysed the autoproteolytic active domain of zucchini yellow mosaic virus (ZYMV) HC-...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2011.097

    authors: Boonrod K,Füllgrabe MW,Krczal G,Wassenegger M

    更新日期:2011-10-01 00:00:00

  • Visualisation of transforming growth factor-beta 1, tissue kallikrein, and kinin and transforming growth factor-beta receptors on human clear-cell renal carcinoma cells.

    abstract::Transforming growth factor-beta1 (TGF-beta1) has a biphasic effect on the growth of renal epithelial cells. In transformed cells, TGF-beta1 appears to accelerate the proliferation of malignant cells. The diverse cellular functions of TGF-beta1 are regulated by three high-affinity serine/threonine kinase receptors, nam...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2005.045

    authors: Moodley R,Snyman C,Odhav B,Bhoola KD

    更新日期:2005-04-01 00:00:00

  • Zinc-dependent changes in oxidative and endoplasmic reticulum stress during cardiomyocyte hypoxia/reoxygenation.

    abstract::Myocardial zinc dyshomeostasis is associated with caspase-3 activation, ErbB2 degradation and apoptosis during hypoxia/reoxygenation. Zinc pyrithione replenishes intracellular zinc, suppresses caspase-3, augments ErbB2 levels and improves cell survival. We hypothesize that zinc is capable of modulating redox and endop...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/hsz-2020-0167

    authors: Bodiga VL,Vemuri PK,Nimmagadda G,Bodiga S

    更新日期:2020-10-25 00:00:00

  • Cross talk between kinin and angiotensin II receptors in mouse abdominal aorta.

    abstract::Bradykinin (BK) is a vasorelaxant, algesic and inflammatory agent. Angiotensin II (AngII) is known to control vascular tone and promote growth, inflammation and artherogenesis. There is evidence for cross talking between BK and AngII receptors. Therefore, the effect of lack of kinin receptors was assessed in mice with...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2009.081

    authors: Rodrigues ES,Martin RP,Felipe SA,Bader M,Oliveira SM,Shimuta SI

    更新日期:2009-09-01 00:00:00