Abstract:
:In bacteria, UGA stop codons can be recoded to direct the incorporation of selenocysteine into proteins on the ribosome. Recoding requires a selenocysteine incorporation sequence (SECIS) downstream of the UGA codon, a specialized translation factor SelB, and the non-canonical Sec-tRNASec, which is formed from Ser-tRNASec by selenocysteine synthase, SelA, using selenophosphate as selenium donor. Here we describe a rapid-kinetics approach to study the mechanism of selenocysteine insertion into proteins on the ribosome. Labeling of SelB, Sec-tRNASec and other components of the translational machinery allows direct observation of the formation or dissociation of complexes by monitoring changes in the fluorescence of single dyes or fluorescence resonance energy transfer between two fluorophores. Furthermore, the structure of SelA was studied by electron cryomicroscopy (cryo-EM). We report that intact SelA from the thermophilic bacterium Moorella thermoacetica (mthSelA) can be vitrified for cryo-EM using a controlled-environment vitrification system. Two-dimensional image analysis of vitrified mthSelA images shows that SelA can adopt the wide range of orientations required for high-resolution structure determination by cryo-EM. The results indicate that mthSelA forms a homodecamer that has a ring-like structure with five bilobed wings, similar to the structure of the E. coli complex determined previously.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Fischer N,Paleskava A,Gromadski KB,Konevega AL,Wahl MC,Stark H,Rodnina MVdoi
10.1515/BC.2007.108subject
Has Abstractpub_date
2007-10-01 00:00:00pages
1061-7issue
10eissn
1431-6730issn
1437-4315journal_volume
388pub_type
杂志文章abstract::Mutations of the p53 gene may alter the specific regulatory domains of the protein. We examined the conserved domains III, IV and V by SSCP using PCR primers covering exons 5, 6, 7 and 8 from hairy cell leukemia (HCL), polyps, colorectal and gastric carcinomas. A low rate of p53 mutations was detected in HCL and polyp...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::The kinin B2 receptor, which is constitutively expressed in a large number of tissues, mediates most of the known effects of bradykinin (BK). Normally undetectable in healthy tissues, the B1 receptor is strongly over-expressed under pathological conditions. BK is an important mediator in renal homeostasis and is mainl...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.004
更新日期:2006-01-01 00:00:00
abstract::Components of kinin-forming systems operating at inflammatory sites are likely to interact with elastase that is released by recruited neutrophils and may, at least temporarily, constitute the major proteolytic activity present at these sites. The aim of this work was to determine the effect of kininogen degradation b...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.104
更新日期:2003-06-01 00:00:00
abstract::A number of unexpected pathophysiological connections linking different neurodegenerative diseases have emerged over the past decade. An example is provided by prion and Alzheimer's diseases. Despite being distinct pathologies, these disorders share several neurotoxic mechanisms, including accumulation of misfolded pr...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2016-0190
更新日期:2016-11-01 00:00:00
abstract::Recent evidence suggests that epidermal growth factor receptor (EGFR)-dependent signaling contributes to liver cell proliferation, as well as to apoptotic liver cell death, and represents an important regulator of hepatic regeneration. This article summarizes recent findings on the molecular mechanisms involved in EGF...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2009.106
更新日期:2009-10-01 00:00:00
abstract::Early diagnostics at the preclinical stage of Alzheimer's disease is of utmost importance for drug development in clinical trials and prognostic guidance. Since soluble Aβ oligomers are considered to play a crucial role in the disease pathogenesis, several methods aim to quantify Aβ oligomers in body fluids such as ce...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2016-0153
更新日期:2017-04-01 00:00:00
abstract::The enzyme L-asparaginase is a crucial component in the treatment of acute lymphoblastic leukemia (ALL). As all asparaginases in clinical use are derived from microorganisms, immunological reactions are the most important adverse events associated with asparaginase treatment. Two different methods, phage display and t...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.063
更新日期:2005-06-01 00:00:00
abstract::We have investigated the methylation status of the repeat unit of the human ribosomal RNA genes in lung cancer. Using a Southern blot analysis approach we have determined that the non-transcribed region of these genes was generally heavily methylated, while the transcribed region was not methylated in either tumor or ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.010
更新日期:1999-01-01 00:00:00
abstract::The thyroglobulin type-1 (Tg-1) domain is a protein module that occurs in a variety of secreted and membrane proteins and is recognised as a potent inhibitor of cysteine peptidases. We present here some properties of the Tg-1 domain of human testican, a modularly organised proteoglycan secreted mainly by brain cells, ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.010
更新日期:2005-01-01 00:00:00
abstract::Abstract Few genes are known to be involved in renal cell carcinoma (RCC) development and progression. The cell-specific transcription factor hepatocyte nuclear factor 4 alpha (HNF4 alpha) is down-regulated in RCC and we have shown that HNF4 alpha inhibits cell proliferation in the embryonic kidney cell line HEK293. T...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.011
更新日期:2008-02-01 00:00:00
abstract::Oncogenic transformation is dependent on activated membrane-associated NADPH oxidase (NOX). However, the resultant extracellular superoxide anions are also driving the NO/peroxynitrite and the HOCl pathway, which eliminates NOX-expressing transformed cells through selective apoptosis induction. Tumor progression is de...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2014-0234
更新日期:2015-12-01 00:00:00
abstract::The cysteine-type peptidase cathepsin X is highly upregulated in several cancers and presumably promotes tumor invasion through bypassing cellular senescence. Here, we present first evidence that the underlying mechanism may involve the regulation of the insulin-like growth factor (IGF) system, a well-known activator ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2012-0209
更新日期:2012-12-01 00:00:00
abstract::The possible application of proteinase inhibitors in the support of anti-tumor chemotherapy requires profound knowledge of the proteinases involved in malignant processes. Therefore, the occurrence of cathepsins B, D, H, L and S and of gelatinases, urokinase plasminogen activator and stromelysins was studied in biopsi...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm3.1996.377.11.695
更新日期:1996-11-01 00:00:00
abstract::L-Arginine:glycine amidinotransferase (AT) catalyzes the committed step in creatine biosynthesis by formation of guanidinoacetic acid, the direct precursor of creatine. The X-ray structure of the human enzyme shows a novel fold with fivefold pseudosymmetry of beta beta alphabeta-modules. These modules enclose the acti...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1997-03-01 00:00:00
abstract::The solute carrier family SLC10 consists of seven members, including the bile acid transporters Na+/taurocholate co-transporting polypeptide (NTCP) and apical sodium-dependent bile acid transporter (ASBT), the steroid sulfate transporter SOAT as well as four orphan carriers (SLC10A3, SLC10A4, SLC10A5 and SLC10A7). Pre...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2019-0148
更新日期:2019-09-25 00:00:00
abstract::Transforming growth factor-β (TGFβ) signaling acts as suppressor and inducer of tumor progression during the early and late stages of cancer, respectively. Some miRNAs have shown a regulatory effect on TGFβ signaling and here, we have used a combination of bioinformatics and experimental tools to show that hsa-miR-559...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2018-0264
更新日期:2019-04-24 00:00:00
abstract::We have characterised a Drosophila P-element enhancer detector insertion F125, which is expressed in the embryonic head and CNS as well as in various third instar imaginal discs. In an attempt to identify the gene with the equivalent expression pattern, we have characterised an adjacent gene. It encodes two novel conc...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1997.378.10.1119
更新日期:1997-10-01 00:00:00
abstract::Host T cell activation, a key step in obtaining adaptive immunity against pathogens, is initiated by the binding of the T cell receptor to a foreign antigenic peptide presented by the major histocompatibility complex on the surface of an antigen-presenting cell and, consequently, formation of an immunological synapse....
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2014-0282
更新日期:2015-06-01 00:00:00
abstract::This review deals with current concepts on the regulation and function of phospholipid asymmetry in biological membranes. This ubiquitous phenomenon is characterized by a distinctly different lipid composition between the inner and outer leaflet of the membrane bilayer. Transbilayer asymmetry is controlled by differen...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1998-08-01 00:00:00
abstract::The gene product of the yeast VPS1 gene is a member of a family of high-molecular-weight GTP-binding proteins that are involved in diverse cellular processes. The Vps1 protein (Vps1p) was shown to perform an essential function in the yeast secretory pathway. Here, we report the isolation and characterization of a muta...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:
更新日期:1997-10-01 00:00:00
abstract::Mammalian tyrosinases are key enzymes of melanin formation. Their native forms undergo complex maturation and sorting processes before being integrated into the melanosomal membrane, which greatly complicates their heterologous expression in other cell types. In the present work, we constructed several differently tru...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2012-0300
更新日期:2013-05-01 00:00:00
abstract::The role of Zn2+-sensing receptor GPR39 on glucose homeostasis and incretin regulation was assessed in enteroendocrine L- and K-cells. Anti-hyperglycaemic, insulinotropic and incretin secreting properties of Zn2+ were explored in normal, diabetic and incretin receptor knockout mice. Compared to intraperitoneal injecti...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2018-0393
更新日期:2019-06-20 00:00:00
abstract::MHC encoded DM heterodimers and classical MHC class II complexes meet in an endosomal/lysosomal compartment where DM heterodimers support peptide loading of MHC class II. Studies on peptide loading of rat class II and on peptide persistence in cells of the dendritic lineage prompted us to establish full length cDNA cl...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1997.378.9.1005
更新日期:1997-09-01 00:00:00
abstract::Reactive oxygen and nitrogen species deposited by cold physical plasma are proposed as predominant effectors in the interaction between discharge and biomedical application. Most reactive species found in plasma sources are known in biology for inter- and intracellular communication (redox signaling) and mammalian cel...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0242
更新日期:2018-12-19 00:00:00
abstract::Synthetic biologists aim at engineering controllable biological parts such as DNA, RNA and proteins in order to steer biological activities using external inputs. Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-t...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0309
更新日期:2019-03-26 00:00:00
abstract::Protein transport, especially into different cellular compartments, is a highly coordinated and regulated process. The molecular machineries which carry out these transport processes are highly complex in structure, function, and regulation. In the case of chloroplasts, thousands of protein molecules have been estimat...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2009.051
更新日期:2009-08-01 00:00:00
abstract::The main function of the epidermis is to establish a vital multifunctional barrier between the body and its external environment. A defective epidermal barrier is one of the key features of atopic dermatitis (AD), a chronic and relapsing inflammatory skin disorder that affects up to 20% of children and 2-3% of adults ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2015-0141
更新日期:2015-11-01 00:00:00
abstract::The enzymatic properties of glycosylphosphatidylinositol-specific phospholipase D (EC 3.1.4.50) were characterized using a 6,000-fold purified enzyme. This was obtained in 100 microg amounts from human serum with a recovery of 35%. Pure alkaline phosphatase containing one anchor moiety per molecule was used as substra...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.062
更新日期:2000-05-01 00:00:00
abstract::We developed a novel method for the simultaneous extraction and analysis of total tissue RNA and DNA to quantify the RNA and DNA oxidation products 8-oxo-7,8-dihydroguanosine and 8-oxo-7,8-dihydro-2'-deoxyguanosine using HPLC coupled to electrochemical detection (HPLC-ECD). The protein denaturing agents guanidine thio...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.014
更新日期:2006-01-01 00:00:00
abstract::Only one transcript for KLK10 was identified by RT-PCR in lung tissue, whereas KLK11 expressed at least four alternative transcripts. Quantitative analysis of KLK10 and KLK11 expression levels was assessed by real-time PCR, in a cohort of 47 patients with non-small-cell lung cancer (NSCLC). Expression levels of these ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.098
更新日期:2006-06-01 00:00:00