The hyaluronan-binding serine protease from human plasma cleaves HMW and LMW kininogen and releases bradykinin.

abstract：:The surface of spherical, nonporous silica nanoparticles (SiO2-NPs) was modified with gadolinium (Gd) complexes, fluorophores, and cell-penetrating peptides to achieve multifunctionality on a single particle. The Gd surface concentrations were 9-16 μmol/g resulting in nanomaterials with high local longitudinal and tra...

journal_title：Biological chemistry

authors： Joshi R,Feldmann V,Koestner W,Detje C,Gottschalk S,Mayer HA,Sauer MG,Engelmann J

更新日期：2013-01-01 00:00:00

abstract：:Type I allergy, a hypersensitivity disease affecting almost 20% of the population worldwide, is based on the IgE recognition of otherwise harmless antigens (i.e., allergens). Allergen-induced crosslink of effector cell-bound IgE antibodies leads to the release of biological mediators and thus to immediate disease symp...

journal_title：Biological chemistry

authors： Valenta R,Vrtala S,Focke-Tejkl M,Bugajska-Schretter,Ball T,Twardosz A,Spitzauer S,Grönlund H,Kraft D

更新日期：1999-07-01 00:00:00

abstract：:The physiological roles of the intracellular iron and redox regulatory systems are intimately linked. Iron is an essential trace element for most organisms, yet elevated cellular iron levels are a potent generator and amplifier of reactive oxygen species and redox stress. Proteins binding iron or iron-sulfur (Fe/S) cl...

journal_title：Biological chemistry

authors： Mühlenhoff U,Braymer JJ,Christ S,Rietzschel N,Uzarska MA,Weiler BD,Lill R

更新日期：2020-11-26 00:00:00

abstract：:The evolution of a highly developed nervous system is mirrored by the ability of individual neurons to develop increased morphological complexity. As microtubules (MTs) are crucially involved in neuronal development, we tested the hypothesis that the evolution of complexity is driven by an increasing capacity of the M...

journal_title：Biological chemistry

authors： Trushina NI,Mulkidjanian AY,Brandt R

更新日期：2019-08-27 00:00:00

abstract：:A controlled interaction of materials with their surrounding biological environment is of great interest in many fields. Multifunctional coatings aim to provide simultaneous modulation of several biological signals. They can consist of various combinations of bioactive, and bioinert components as well as of reporter m...

journal_title：Biological chemistry

authors： Pagel M,Beck-Sickinger AG

更新日期：2017-01-01 00:00:00

abstract：:The gene product of the yeast VPS1 gene is a member of a family of high-molecular-weight GTP-binding proteins that are involved in diverse cellular processes. The Vps1 protein (Vps1p) was shown to perform an essential function in the yeast secretory pathway. Here, we report the isolation and characterization of a muta...

journal_title：Biological chemistry

authors： Finken-Eigen M,Müller S,Köhrer K

更新日期：1997-10-01 00:00:00

abstract：:Cysteine cathepsins are expressed in most tissues, including the gastrointestinal tract. We demonstrated an involvement of mouse intestinal cathepsin B in extracellular matrix remodeling for regeneration from trauma. The present study aimed at elucidating roles of cysteine cathepsins in the non-traumatized gastrointes...

journal_title：Biological chemistry

authors： Tamhane T,Arampatzidou M,Gerganova V,Tacke M,Illukkumbura R,Dauth S,Schaschke N,Peters C,Reinheckel T,Brix K

更新日期：2014-10-01 00:00:00

abstract：:The p53 tumour suppressor gene is frequently mutated in human tumours and different tumour-derived mutations have varying effects on cells. The effect of a novel tumour-derived p53 mutation and two recently described mutations from South African breast cancer patients on the growth rate, colony formation, cell cycle a...

journal_title：Biological chemistry

authors： Donninger H,Binder A,Bohm L,Parker MI

更新日期：2008-01-01 00:00:00

abstract：:DEAD-box proteins disrupt or remodel RNA and protein/RNA complexes at the expense of ATP. The catalytic core is composed of two flexibly connected RecA-like domains. The N-terminal domain contains most of the motifs involved in nucleotide binding and serves as a minimalistic model for helicase/nucleotide interactions....

journal_title：Biological chemistry

authors： Strohmeier J,Hertel I,Diederichsen U,Rudolph MG,Klostermeier D

更新日期：2011-04-01 00:00:00

abstract：:Cysteine proteases of the malaria parasite Plasmodium falciparum, known as falcipains, are promising targets for antimalarial chemotherapy. We evaluated cultured parasites for the stage-specific expression of cysteine proteases and sensitivity to cysteine protease inhibitors. Protease activity and inhibitor sensitivit...

journal_title：Biological chemistry

authors： Shenai BR,Semenov AV,Rosenthal PJ

更新日期：2002-05-01 00:00:00

abstract：:We have previously described Kunitz-type serine proteinase inhibitors purified from Bauhinia seeds. Human plasma kallikrein shows different susceptibility to those inhibitors. In this communication, we describe the interaction of human plasma kallikrein with fluorogenic and non-fluorogenic peptides based on the Bauhin...

journal_title：Biological chemistry

authors： Oliva ML,Santomauro-Vaz EM,Andrade SA,Juliano MA,Pott VJ,Sampaio MU,Sampaio CA

更新日期：2001-01-01 00:00:00

abstract：:The flagellate Euglena gracilis contains as photoreceptor complex a heterotetrameric light-sensitive adenylyl cyclase (AC), consisting of the flavoproteins PACalpha and PACbeta. Previously, we demonstrated the functional expression of PACalpha and PACbeta in oocytes from Xenopus laevis and of PACalpha in different ani...

journal_title：Biological chemistry

authors： Looser J,Schröder-Lang S,Hegemann P,Nagel G

更新日期：2009-11-01 00:00:00

abstract：:Chitinase-like proteins (CLPs) are lectins combining properties of cytokines and growth factors. Human CLPs include YKL-40, YKL-39 and SI-CLP that are secreted by cancer cells, macrophages, neutrophils, synoviocytes, chondrocytes and other cells. The best investigated CLP in cancer is YKL-40. Serum and plasma levels o...

journal_title：Biological chemistry

authors： Kzhyshkowska J,Yin S,Liu T,Riabov V,Mitrofanova I

更新日期：2016-03-01 00:00:00

abstract：:The transcription factor GLI1 is one of three vertebrate members of the GLI family, which is characterized by a highly conserved DNA-binding domain of five zinc fingers. We have analyzed whether human GLI1 is a target of PKA regulation. It was found that PKA inhibits GLI1 transcriptional activity. However, no evidence...

journal_title：Biological chemistry

authors： Kaesler S,Lüscher B,Rüther U

更新日期：2000-07-01 00:00:00

abstract：:Parvulins belong to the family of peptidyl-prolyl cis/trans isomerases (PPIases) assisting in protein folding and in regulating the function of a broad variety of proteins in all branches of life. The human representatives Pin1 and Par14/17 are directly involved in processes influencing cellular maintenance and cell f...

journal_title：Biological chemistry

authors： Matena A,Rehic E,Hönig D,Kamba B,Bayer P

更新日期：2018-01-26 00:00:00

abstract：:Malaria can be a very severe disease, particularly in young children, pregnant women (mostly in primipara), and malaria naïve adults, and currently ranks among the most prevalent infections in tropical and subtropical areas throughout the world. The widespread occurrence and the increased incidence of malaria in many ...

journal_title：Biological chemistry

authors： Tsuji M,Rodrigues EG,Nussenzweig S

更新日期：2001-04-01 00:00:00

abstract：:A novel approach to studying the inter- and intrasubunit communication required for the activity of homodimeric proteins is described. It was developed for the restriction endonuclease EcoRV, but should also be useful for other homodimeric enzymes. Two ecorV genes encoding different EcoRV mutants are coexpressed in th...

journal_title：Biological chemistry

authors： Wende W,Stahl F,Pingoud A

更新日期：1996-10-01 00:00:00

abstract：:The glutamyl endopeptidase family of enzymes from staphylococci has been shown to be important virulence determinants of pathogenic family members, such as Staphylococcus aureus. Previous studies have identified the N-terminus and residues from positions 185-195 as potentially important regions that determine the acti...

journal_title：Biological chemistry

authors： Ono T,Ohara-Nemoto Y,Shimoyama Y,Okawara H,Kobayakawa T,Baba TT,Kimura S,Nemoto TK

更新日期：2010-10-01 00:00:00

abstract：:To elucidate the structures and dynamics of membrane proteins, highly advanced biophysical methods have been developed that often require significant resources, both for sample preparation and experimental analyses. For very complex systems, such as membrane transporters, ion channels or G-protein coupled receptors (G...

journal_title：Biological chemistry

authors： Rose-Sperling D,Tran MA,Lauth LM,Goretzki B,Hellmich UA

更新日期：2019-09-25 00:00:00

abstract：:The transcription of the fatty acid synthase (FAS) gene is regulated by the sterol status of the cell via cleavage of the sterol regulatory element-binding protein (SREBP). When human HepG2 hepatoma cells were cotransfected with an expression plasmid for mature SREBP-1a together with FAS promoter/reporter constructs s...

journal_title：Biological chemistry

authors： Wolf SS,Roder K,Sickinger S,Schweizer M

更新日期：2001-07-01 00:00:00

abstract：:Kallikrein-related peptidase 2 (KLK2) degrades insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) in vitro. IGFBP-3 forms complexes with IGFs, preventing them from binding to their receptors and stimulating cell proliferation and survival. IGF-independent actions have also been described for IGFBP-3. The deg...

journal_title：Biological chemistry

authors： Hekim C,Riipi T,Weisell J,Närvänen A,Koistinen R,Stenman UH,Koistinen H

更新日期：2010-04-01 00:00:00

abstract：:As the outermost barrier of the body, the skin is directly and frequently exposed to a prooxidative environment, including solar UVA and UVB radiation, and air pollution. Ozone, a major air pollutant, possesses a strong oxidizing potential and therefore is possibly one of the most reactive chemicals the skin ever meet...

journal_title：Biological chemistry

authors： Thiele JJ,Podda M,Packer L

更新日期：1997-11-01 00:00:00

abstract：:The saline extract of Bauhinia bauhinioides dry seeds was shown to inhibit cruzipain, a cysteine proteinase from Trypanosoma cruzi. The inhibitory activity was assigned to a protein with 164 amino acid residues and molecular mass of 18 034 Da that was purified by chromatography on DEAE-Sephadex, trypsin-Sepharose (rem...

journal_title：Biological chemistry

authors： de Oliveira C,Santana LA,Carmona AK,Cezari MH,Sampaio MU,Sampaio CA,Oliva ML

更新日期：2001-05-01 00:00:00

abstract：:It is not rare that controversial indications about the presence or the expression level of multidrug-resistant (MDR) proteins come out from different laboratories upon examination of identical tumor specimens. Distinct aspects, including the use of weakly discriminating monoclonal antibodies (MAbs) and/or unsuitable ...

journal_title：Biological chemistry

authors： Poloni F,Castagna M,Felici F,Cianfriglia M

更新日期：1997-06-01 00:00:00

abstract：:TNF-alpha is a major pro-inflammatory cytokine that regulates further cytokine induction, especially of IL-1 and IL-6, in many human diseases including cancer, inflammation and immune disorders. In a search for new inhibitors of inducible TNF-alpha promoter activity and expression, cultures of the imperfect fungus Tri...

journal_title：Biological chemistry

authors： Rether J,Serwe A,Anke T,Erkel G

更新日期：2007-06-01 00:00:00

abstract：:The kallikrein-kinin and renin-angiotensin (KKS-RAS) systems represent two highly regulated proteolytic systems that are involved in several physiological and pathological processes. Although their protein-protein interactions can be studied using experimental approaches, it is difficult to differentiate between direc...

journal_title：Biological chemistry

authors： Stoka V,Turk V

更新日期：2010-04-01 00:00:00

abstract：:The primary cilium is a sensory organelle that is vital in regulating several signalling pathways. Unlike most organelles cilia are open to the rest of the cell, not enclosed by membranes. The distinct protein composition is crucial to the function of cilia and many signalling proteins and receptors are specifically c...

journal_title：Biological chemistry

authors： Stephen LA,Elmaghloob Y,Ismail S

更新日期：2017-12-20 00:00:00

abstract：:The surface topography of red blood cells (RBCs) was investigated under near-physiological conditions using atomic force microscopy (AFM). An immobilization protocol was established where RBCs are coupled via molecular bonds of the membrane glycoproteins to wheat germ agglutinin (WGA), which is covalently and flexibly...

journal_title：Biological chemistry

authors： Kamruzzahan AS,Kienberger F,Stroh CM,Berg J,Huss R,Ebner A,Zhu R,Rankl C,Gruber HJ,Hinterdorfer P

更新日期：2004-10-01 00:00:00

abstract：:Human adenocarcinomas overexpress a hypoglycosylated, tumor-associated form of the mucin-like glycoprotein MUC1 containing abnormal mono- and disaccharide antigens, such as Tn, sialyl-Tn, and TF, as well as stretches of unglycosylated protein backbone in the variable number of tandem repeats (VNTR) region. Both peptid...

journal_title：Biological chemistry

authors： Ryan SO,Vlad AM,Islam K,Gariépy J,Finn OJ

更新日期：2009-07-01 00:00:00

abstract：:A kinetic study was performed of a model for an autocatalytic zymogen activation process involving both intra- and intermolecular routes, to which a chromogenic reaction in which the active enzyme acts upon one of its substrates was coupled to continuously monitor the reaction. Kinetic equations describing the evoluti...

journal_title：Biological chemistry

authors： Fuentes ME,Varón R,García-Moreno M,Valero E

更新日期：2005-07-01 00:00:00