Degradation of channelopsin-2 in the absence of retinal and degradation resistance in certain mutants.

abstract：:Functional ribonuclease A was presented on the surface of the filamentous phage M13 by fusion to the minor coat protein. RNase activity of the fusion protein was shown by a zymogram assay. In addition, we established a modified method for preparing RNase-displaying phages without contaminating host RNases. ...

journal_title：Biological chemistry

authors： Korn K,Foerster HH,Hahn U

更新日期：2000-02-01 00:00:00

abstract：:Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes. ...

journal_title：Biological chemistry

authors： Czerney P,Lehmann F,Wenzel M,Buschmann V,Dietrich A,Mohr GJ

更新日期：2001-03-01 00:00:00

abstract：:Nitrogen dioxide (*NO2) is an oxidizing free radical which can initiate a variety of destructive pathways in living systems, and several diseases are suspected to be connected with both exogenously and endogenously formed *NO2. Peroxynitrite (ONOO-/ONOOH) is believed to be an important endogenous source of *NO2 radica...

journal_title：Biological chemistry

authors： Kirsch M,Korth HG,Sustmann R,de Groot H

更新日期：2002-03-01 00:00:00

abstract：:Forkhead box O (FoxO) transcription factors are important downstream targets of the PI3K/Akt signaling pathway and crucial regulators of cell fate. This function of FoxOs relies on their ability to control diverse cellular functions, including proliferation, differentiation, apoptosis, DNA repair, defense against oxid...

journal_title：Biological chemistry

authors： Sedding DG

更新日期：2008-03-01 00:00:00

abstract：:Human septins comprise a family of 13 genes that encode for >30 protein isoforms with ubiquitous and tissue-specific expressions. Septins are GTP-binding proteins that assemble into higher-order oligomers and filamentous polymers, which associate with cell membranes and the cytoskeleton. In the last decade, much progr...

journal_title：Biological chemistry

authors： Dolat L,Hu Q,Spiliotis ET

更新日期：2014-02-01 00:00:00

abstract：:Interleukin-13 (IL-13) is a T-cell-derived pleiotropic cytokine of particular medical importance because of its critical role in the development of allergic asthma. The effects of IL-13 on its target cells are mediated through a dimeric transmembrane receptor (IL-13R), which shares the IL-4Ralpha subunit with the IL-4...

journal_title：Biological chemistry

authors： Knackmuss S,Krause S,Engel K,Reusch U,Virchow JC,Mueller T,Kraich M,Little M,Luttmann W,Friedrich K

更新日期：2007-03-01 00:00:00

abstract：:Abstract Fanconi anemia (FA) is a rare cancer-prone genetic disorder characterized by progressive bone marrow failure, chromosomal instability and redox abnormalities. There is much biochemical and genetic data, which strongly suggest that FA cells experience increased oxidative stress. The present study was designed ...

journal_title：Biological chemistry

authors： Petrovic S,Leskovac A,Kotur-Stevuljevic J,Joksic J,Guc-Scekic M,Vujic D,Joksic G

更新日期：2011-07-01 00:00:00

abstract：:TNF-alpha is a major pro-inflammatory cytokine that regulates further cytokine induction, especially of IL-1 and IL-6, in many human diseases including cancer, inflammation and immune disorders. In a search for new inhibitors of inducible TNF-alpha promoter activity and expression, cultures of the imperfect fungus Tri...

journal_title：Biological chemistry

authors： Rether J,Serwe A,Anke T,Erkel G

更新日期：2007-06-01 00:00:00

abstract：:Dendritic cells (DCs) are an important link between innate and adaptive immunity. DCs get activated in inflamed tissues where oxygen tension is usually low, which requires the transcription factor hypoxia inducible factor (HIF)-1 for cellular adaptation. To investigate whether the HIF-1 transcriptional complex plays a...

journal_title：Biological chemistry

authors： Wobben R,Hüsecken Y,Lodewick C,Gibbert K,Fandrey J,Winning S

更新日期：2013-04-01 00:00:00

abstract：:Trypsin and chymotrypsin have similar tertiary structures, although very different substrate specificities. Trypsin hydrolyzes peptides at Lys/Arg residues while chymotrypsin recognizes large hydrophobic residues. Recent work has shown that trypsin is not converted into a protease with chymotrypsin-like activity when ...

journal_title：Biological chemistry

authors： Hedstrom L

更新日期：1996-07-01 00:00:00

abstract：:Full-length Hsp70 protein (Hsp70) and the C-terminal domain of Hsp70 (Hsp70C) both stimulate the cytolytic activity of naive natural killer (NK) cells against Hsp70-positive tumor target cells. Here, we describe the characterization of Hsp70-NK cell interaction with binding studies using the human NK cell line YT. Bin...

journal_title：Biological chemistry

authors： Gross C,Hansch D,Gastpar R,Multhoff G

更新日期：2003-02-01 00:00:00

abstract：:Here, we describe a novel member in the group of membrane-anchored chymotrypsin (S1)-like serine proteases, namely testis serine protease 1 (T-SP1), as it is principally expressed in testis tissue. The human T-SP1 gene encompasses 28.7 kb on the short arm of chromosome 8 and consists of seven exons. Rapid amplificatio...

journal_title：Biological chemistry

authors： Neth P,Profanter B,Geissler C,Nägler DK,Nerlich A,Sommerhoff CP,Jochum M

更新日期：2008-12-01 00:00:00

abstract：:The kallikrein-related peptidase (KLK) gene family of 15 serine proteases encodes many proteins, including prostate specific antigen (PSA or KLK3), that are well described and/or are potential biomarkers for hormone-related cancers. Variant mRNA transcripts produced by alternative splicing, polyadenylation or AUG site...

journal_title：Biological chemistry

authors： Tan OL,Whitbread AK,Clements JA,Dong Y

更新日期：2006-06-01 00:00:00

abstract：:Glycoconjugates have a whole spectrum of biological roles, from those that appear trivial to those that are crucial. Results accumulated in the past years indicate they might also play an important role in the response to stress, a complex physiological response of the human organism to various threats. We have recent...

journal_title：Biological chemistry

authors： Lauc G,Peter-Katalinić J,Dabelić S,Flögel M

更新日期：1999-04-01 00:00:00

abstract：:As an important protein, UL31 has been demonstrated to play multiple roles in herpes simplex virus 1 (HSV-1) replication. Previous studies showed that UL31 predominantly locates in the nucleus in chemical fixed cells and live cells, however, the determining mechanisms for its nuclear translocation is not clear. In the...

journal_title：Biological chemistry

authors： Cai M,Si J,Li X,Zeng Z,Li M

更新日期：2016-06-01 00:00:00

abstract：:We have investigated the methylation status of the repeat unit of the human ribosomal RNA genes in lung cancer. Using a Southern blot analysis approach we have determined that the non-transcribed region of these genes was generally heavily methylated, while the transcribed region was not methylated in either tumor or ...

journal_title：Biological chemistry

authors： Shiraishi M,Sekiguchi A,Chuu YH,Sekiya T

更新日期：1999-01-01 00:00:00

abstract：:Proteomic identification of protease cleavage site specificity (PICS) is a recent proteomic approach for the easy mapping of protease subsite preferences that determines both the prime- and non-prime side specificity concurrently. Here we greatly facilitate user access by providing an automated and simple web-based da...

journal_title：Biological chemistry

authors： Schilling O,auf dem Keller U,Overall CM

更新日期：2011-11-01 00:00:00

abstract：:Adenoviral DNA was examined within capsids by dimethyl sulfate footprinting. Protein-DNA interactions were visualized through ligation-mediated PCR (LM-PCR). Signals for protein binding were found adjacent to both inverted terminal repeats (ITR). There were no indications of close protein binding at several other loci...

journal_title：Biological chemistry

authors： Dobner T,Büchner D,Zeller T,Wolf H,Niller HH

更新日期：2001-09-01 00:00:00

abstract：:Bradykinin causes vascular relaxations through release of endothelial relaxing factors including prostacyclin, nitric oxide (NO) and epoxyeicosatrienoic acids (EETs). Bradykinin is metabolized by angiotensin converting enzyme (ACE) and ACE inhibition enhances bradykinin relaxations. Our goal was to characterize the ro...

journal_title：Biological chemistry

authors： Gauthier KM,Cepura CJ,Campbell WB

更新日期：2013-09-01 00:00:00

abstract：:Historically considered as accessory cells to neurons, there is an increasing interest in the role of astrocytes in normal and pathological conditions. Astrocytes are involved in neurotransmitter recycling, antioxidant supply, ion buffering and neuroinflammation, i.e. a lot of the same pathways that go astray in Alzhe...

journal_title：Biological chemistry

authors： Zulfiqar S,Garg P,Nieweg K

更新日期：2019-08-27 00:00:00

abstract：:Characterization of protein damage during photosensitization of chlorin e6-treated cells was performed using the green fluorescent protein (GFP). The GFP-chromophore damage caused by singlet oxygen was studied in COS 7 kidney cells and E. coli bacteria following light irradiation. Electron spin resonance (ESR) reveale...

journal_title：Biological chemistry

authors： Greenbaum L,Rothmann C,Lavie R,Malik Z

更新日期：2000-12-01 00:00:00

abstract：:A central point of regulation in the Wnt/beta-catenin signalling pathway is the formation of the beta-catenin destruction complex. Axin1, an essential negative regulator of Wnt signalling, serves as a scaffold within this complex and is critical for rapid turnover of beta-catenin. To examine the mechanism by which Wnt...

journal_title：Biological chemistry

authors： Sue Ng S,Mahmoudi T,Li VS,Hatzis P,Boersema PJ,Mohammed S,Heck AJ,Clevers H

更新日期：2010-02-01 00:00:00

abstract：:Disulfide bonds play a critical role in a variety of structural and mechanistic processes associated with proteins inside the cells and in the extracellular environment. The thioredoxin family of proteins like thioredoxin (Trx), glutaredoxin (Grx) and protein disulfide isomerase, are involved in the formation, transfe...

journal_title：Biological chemistry

authors： Sousa SF,Neves RPP,Waheed SO,Fernandes PA,Ramos MJ

更新日期：2019-04-24 00:00:00

abstract：:Drug-induced phospholipidosis is a lysosomal storage disorder characterized by excessive accumulation of phospholipids. Its cellular mechanism is still not well understood, but it is known that cationic amphiphilic drugs can induce it. These drugs have a hydrophilic amine head group that can be protonated in the endol...

journal_title：Biological chemistry

authors： Breiden B,Sandhoff K

更新日期：2019-12-18 00:00:00

abstract：:The surface topography of red blood cells (RBCs) was investigated under near-physiological conditions using atomic force microscopy (AFM). An immobilization protocol was established where RBCs are coupled via molecular bonds of the membrane glycoproteins to wheat germ agglutinin (WGA), which is covalently and flexibly...

journal_title：Biological chemistry

authors： Kamruzzahan AS,Kienberger F,Stroh CM,Berg J,Huss R,Ebner A,Zhu R,Rankl C,Gruber HJ,Hinterdorfer P

更新日期：2004-10-01 00:00:00

abstract：:Based on studies presented here and other published experiments performed with surviving tissue preparations, with transfected cells and with cells that constitutively express the human angiotensin I converting enzyme ACE and B2 receptors, we concluded the following: ACE inhibitors and other endogenous peptides that r...

journal_title：Biological chemistry

authors： Erdös EG,Marcic BM

更新日期：2001-01-01 00:00:00

abstract：:As the outermost barrier of the body, the skin is directly and frequently exposed to a prooxidative environment, including solar UVA and UVB radiation, and air pollution. Ozone, a major air pollutant, possesses a strong oxidizing potential and therefore is possibly one of the most reactive chemicals the skin ever meet...

journal_title：Biological chemistry

authors： Thiele JJ,Podda M,Packer L

更新日期：1997-11-01 00:00:00

abstract：:Synthetic biologists aim at engineering controllable biological parts such as DNA, RNA and proteins in order to steer biological activities using external inputs. Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-t...

journal_title：Biological chemistry

authors： Di Ventura B,Mootz HD

更新日期：2019-03-26 00:00:00

abstract：:Coxsackievirus B3 (CVB-3) is a plus-strand RNA virus that is believed to be the most common causal agent of viral myocarditis. Since no specific treatment for CVB-3 infections is available to date, we and others have recently started to develop RNA interference (RNAi) approaches to prevent virus propagation. Here we d...

journal_title：Biological chemistry

authors： Werk D,Schubert S,Lindig V,Grunert HP,Zeichhardt H,Erdmann VA,Kurreck J

更新日期：2005-09-01 00:00:00

abstract：:Plasma prekallikrein (PPK) is synthesised in hepatocytes and secreted into the blood, where it participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. Recently we demonstrated by quantitative RT-PCR that the human PPK gene is transcribed not only in the l...

journal_title：Biological chemistry

authors： Fink E,Bhoola KD,Snyman C,Neth P,Figueroa CD

更新日期：2007-09-01 00:00:00