Virus shedding by SV40-transformed human cells.

Abstract:

:Cultures of human cells transformed by SV40 were found to release infectious virus, even after several passages in vitro. Virus shedding by these cultures did not depend on propagation of virus from cell to cell, as it was not affected by anti-SV40 antiserum that could effectively block virus propagation in acutely infected cells. Cells transformed by the 'early' temperature-sensitive mutant tsA30, and maintained at the restrictive temperature of 39 degrees, shed virus in reduced amount. Finally, xeroderma pigmentosum cells transformed by SV40 were also found to release virus, indicating that the enzymes of excision and repair of UV-induced damage to DNA probably were not involved in the molecular mechanism underlying virus shedding.

journal_name

Intervirology

journal_title

Intervirology

authors

Lomax CA,Thirion JP,Bourgaux-Ramoisy D

doi

10.1159/000148919

subject

Has Abstract

pub_date

1978-01-01 00:00:00

pages

39-47

issue

1

eissn

0300-5526

issn

1423-0100

journal_volume

9

pub_type

杂志文章
  • Prevalence and Clinical Profile of Human Salivirus in Children with Acute Gastroenteritis in Northern Italy, 2014-2015.

    abstract:OBJECTIVE:Human Salivirus (SalV) has been associated with gastroenteritis on all continents. METHODS:This paper presents the real-time RT-PCR assay for the detection of SalV in clinical fecal samples collected from 192 hospitalized children with acute gastroenteritis in Piedmont, Italy. RESULTS:The most commonly dete...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000490568

    authors: Bergallo M,Daprà V,Rassu M,Bonamin S,Cuccu R,Calvi C,Montanari P,Galliano I

    更新日期:2018-01-01 00:00:00

  • Determination of drug resistance and virus typology in HIV-1-positive pediatric patients in Istanbul, Turkey.

    abstract::The aim of the study was to determine the prevalence of drug resistance of HIV-1 in pediatric patients from Istanbul, Turkey. Genotypic drug resistance testing revealed transmission of drug resistance from mother to child in 20%. Due to rising numbers of children with HIV-1, baseline resistance testing is recommended ...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000362335

    authors: Yoldaş O,Ağaçfidan A,Lübke N,Somer A,Hançerli S,Verheyen J,Kaiser R,Akgül B

    更新日期:2014-01-01 00:00:00

  • Clinical, Virological, and Immunological Profiles of DENV, ZIKV, and/or CHIKV-Infected Brazilian Patients.

    abstract:BACKGROUND:Arboviruses co-circulating within a population that are transmitted by the same vector have the potential to cause coinfections. Coinfections with dengue virus (DENV), Zika virus (ZIKV), and chikungunya virus (CHIKV) have been occurring in Brazil, but it is not well-understood how human responses vary during...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000510223

    authors: Sánchez-Arcila JC,Badolato-Correa J,de Souza TMA,Paiva IA,Barbosa LS,Nunes PCG,Lima MDRQ,Dos Santos FB,Damasco PV,da Cunha RV,Azeredo EL,de Oliveira-Pinto LM

    更新日期:2020-01-01 00:00:00

  • Inhibition of Mayaro virus replication by prostaglandin A(1) in Vero cells.

    abstract::Prostaglandins exhibit antiviral activity against a wide variety of RNA and DNA viruses. In the present report, we describe the effect of cyclopentenone prostaglandin A(1) (PGA(1)) on Mayaro virus replication in Vero cells. Virus yield was significantly reduced at nontoxic concentrations which did not suppress DNA, RN...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000050069

    authors: Burlandy FM,Rebello MA

    更新日期:2001-01-01 00:00:00

  • Historical Discoveries on Viruses in the Environment and Their Impact on Public Health.

    abstract:BACKGROUND:Transmission of many viruses occurs by direct transmission during a close contact between two hosts, or by an indirect transmission through the environment. Several and often interconnected factors, both abiotic and biotic, determine the persistence of these viruses released in the environment, which can las...

    journal_title:Intervirology

    pub_type: 历史文章,杂志文章,评审

    doi:10.1159/000511575

    authors: Labadie T,Batéjat C,Leclercq I,Manuguerra JC

    更新日期:2020-01-01 00:00:00

  • Application of the critical point dried whole cell technique to the study of animal rhabdoviruses.

    abstract::The critical point dried (CPD) whole cell technique was applied to the study of the morphogenesis and morphology of rabies virus and vesicular stomatitis virus (VSV) in mouse neuroblastoma and baby hamster kidney (BHK) cells. With the stereoscopic technique, progeny viruses at the cell surface and within the cytoplasm...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000148939

    authors: Iwasaki Y

    更新日期:1978-01-01 00:00:00

  • Diagnostic value of nucleic-acid-sequence- based amplification for the detection of cytomegalovirus infection in renal and liver transplant recipients.

    abstract::To evaluate the diagnostic value of nucleic-acid-sequence-based amplification (NASBA) for the detection of cytomegalovirus (CMV) infection in transplant recipients, we compared immediate early 1 (IE1) and late pp67 mRNA detection by NASBA with the antigenemia assay, PCR and viral culture in 72 renal transplant (RTx) r...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000053974

    authors: Goossens VJ,Blok MJ,Christiaans MH,van Hooff JP,Sillekens P,Höckerstedt K,Lautenschlager I,Middeldorp JM,Bruggeman CA

    更新日期:1999-01-01 00:00:00

  • Molecular characterization of Jurkat cells persistently infected with vaccinia virus mutant vp811.

    abstract::Previous reports from this laboratory demonstrated that persistent infections could be established in Jurkat cells with wild-type vaccinia virus (Jvac). The infected cells elicit increased expression of IL-2, IL-2Ralpha and IL-6 but not of IL-1beta nor of interferon-gamma. The persistently infected cells are also capa...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000081734

    authors: Melana SM,Pogo BG

    更新日期:2005-01-01 00:00:00

  • Experimental and spontaneous mucosal disease of cattle: a validation of Koch's postulates in the definition of pathogenesis.

    abstract::The complex etiology of mucosal disease of cattle has been unravelled by experimental reproduction of the fatal syndrome, thereby validating Koch's postulates. The causal virus, bovine virus diarrhea virus (BVDV), has two biotypes, noncytopathogenic and cytopathogenic; the former can infect the early bovine fetus, ind...

    journal_title:Intervirology

    pub_type: 杂志文章,评审

    doi:10.1159/000150295

    authors: Brownlie J,Clarke MC

    更新日期:1993-01-01 00:00:00

  • Measurement of human cytomegalovirus-associated DNA polymerase activity in patient urine as a potential diagnostic tool.

    abstract::Virus-associated DNA polymerase activity has recently been proposed for the detection of human cytomegalovirus (HCMV) in urine, a method that should allow rapid and quantitative determination of the viral load. In this report, the virus-associated DNA polymerase activity recovered from the urine of a group of patients...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000150530

    authors: Marcello A,Pisoni G,Pizzighella S,Palù G

    更新日期:1996-01-01 00:00:00

  • Enhancement of Epstein-Barr virus gene expression by other herpesviruses.

    abstract::We examined the effect of infection by other herpesviruses on the induction of Epstein-Barr virus (EBV) antigens. Raji cells were infected with a variety of viruses before superinfection with P3HR-1 virus 6 h later. Much earlier and greater synthesis of EBV antigens was observed in herpesvirus (herpes simplex virus ty...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000150057

    authors: Kikuta H,Rapp F

    更新日期:1988-01-01 00:00:00

  • Efficient ribosome binding of brome mosaic virus (BMV) RNA4 contributes to its ability to outcompete the other BMV RNAs for translation.

    abstract::Analysis of translation products synthesized in vitro in the presence of a mixture of brome mosaic virus (BMV) RNAs 1, 2, 3, and 4 usually shows a predominance of coat protein, coded by RNA4. The proportion of products directed by RNAs 1 and 2 decreased at saturating concentrations of BMV RNA and in the presence of 7-...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149008

    authors: Pyne JW,Hall TC

    更新日期:1979-01-01 00:00:00

  • Monkeys and Rats Are Not Susceptible to Ferret Hepatitis E Virus Infection.

    abstract::Ferret hepatitis E virus (HEV), a novel hepatitis E-like virus, has been identified in ferrets in the Netherlands, Japan, and the US. To determine whether ferret HEV transmits to other animals, we inoculated laboratory rats (Wistar), nude rats (Long-Evans-rnu/rnu), and cynomolgus monkeys with ferret HEV (F4351) by int...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000373891

    authors: Li TC,Yoshizaki S,Ami Y,Suzaki Y,Yang T,Takeda N,Takaji W

    更新日期:2015-01-01 00:00:00

  • Hepatitis B virus genotypes and hepatocellular carcinoma in Japan.

    abstract::Hepatitis B virus (HBV) is one of the major causative agents of hepatocellular carcinoma (HCC) in Southeast Asia where HBV genotype B (HBV/B) and genotype C (HBV/C) are prevalent. Clinical and virological differences were evaluated between HCC patients in Japan and Taiwan, all of whom were infected with HBV/B and HBV/...

    journal_title:Intervirology

    pub_type: 杂志文章,评审

    doi:10.1159/000075000

    authors: Orito E,Mizokami M

    更新日期:2003-01-01 00:00:00

  • Genotype analysis, using PCR with type-specific primers, of hepatitis B virus isolates from patients coinfected with hepatitis delta virus genotype II from Miyako Island, Japan.

    abstract:OBJECTIVES:The aims of this study were to determine the hepatitis B virus (HBV) genotypes in hepatitis delta virus (HDV) RNA-positive patients and to characterize the HBV nucleotide sequences that may be found on a distant island of Japan. METHODS:This study included three patients with chronic hepatitis who were posi...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000069746

    authors: Moriyama M,Taira M,Matsumura H,Aoki H,Mikuni M,Kaneko M,Shioda A,Iwaguchi K,Arai S,Ichijima S,Iwasaki H,Tanaka N,Abe K,Arakawa Y

    更新日期:2003-01-01 00:00:00

  • Growth characteristics of acute hemorrhagic conjunctivitis (AHC) virus in monkey kidney cells. II. Temperature sensitivity of the isolates obtained at various epidemic areas.

    abstract::Acute hemorrhagic conjunctivitis (AHC) virus strains isolated in eight different areas during epidemics of AHC were tested for the reproductive capacity at 33, 37 and 39 degrees. All of the 25 strains tested grew better at 33 degrees but restrictively at 39 degrees. The degree of temperature sensitivity varied slightl...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149952

    authors: Miyamura K,Sasagawa A,Tajiri E,Kono R

    更新日期:1976-01-01 00:00:00

  • Havachoobe (Onosma dichroanthum Boiss) Root Extract Decreases the Hepatitis B Virus Surface Antigen Secretion in the PLC/PRF/5 Cell Line.

    abstract:BACKGROUND:Many efforts are currently focused on functional treatment of the hepatitis B virus (HBV). This can be done by suppressing the secretion of HBV surface antigen (HBsAg). Scientific communities are very interested in natural products in that respect. OBJECTIVE:Use of root extract of Havachoobe (Onosma dichroa...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000512140

    authors: Mohebbi A,Azadi F,Hashemi MM,Askari FS,Razzaghi N

    更新日期:2020-12-15 00:00:00

  • Biochemically silent posttransfusion non-A, non-B hepatitis interferes with superinfection by hepatitis A virus.

    abstract::Superinfection of a silently non-A, non-B (NANB)-infected chimpanzee with hepatitis A virus (HAV) resulted in minimal liver enzyme elevations, lack of detectable HAV in stool, and questionable presence of HAV antigen in liver biopsy specimens obtained during the expected period of virus replication. Our findings indic...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149724

    authors: Bradley DW,Krawczynski K,Humphrey CD,McCaustland KA

    更新日期:1987-01-01 00:00:00

  • Effect of glucosamine on the replication of the arenavirus Junín in Vero cells.

    abstract::When Vero cells infected with Junín virus are cultured in the presence of 16 mM glucosamine, no infectious virus is produced and no surface antigens or virions are detected. Cytoplasmic viral antigen, however, is synthesized normally. ...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149032

    authors: Martínez Peralta LA,León ME,Coto CE,Laguens RP

    更新日期:1979-01-01 00:00:00

  • Loss of immunorecessive cytotoxic T lymphocyte determinant V on SV40 T antigen following cocultivation with site-specific cytotoxic T lymphocyte clone Y-5.

    abstract::Five distinct antigenic sites recognized by cytotoxic T lymphocytes in H-2b mice have been identified on simian virus 40 (SV40) T antigen. Cocultivation of SV40-transformed cells with cytotoxic T lymphocyte (CTL) clones specific for epitopes I-IV results in the selection of variants which show a loss of either epitope...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000150154

    authors: Tanaka Y,Tevethia SS

    更新日期:1990-01-01 00:00:00

  • Suckling mice were used to detect infectious dengue-2 viruses by intracerebral injection of the full-length RNA transcript.

    abstract:OBJECTIVE:In this study we established a mouse brain injection system to detect infectious dengue-2 virus produced from the full-length RNA transcripts. METHODS:In vitro transcription was used to synthesize full-length dengue-2 virus RNA from the plasmid pRS424FLDEN2NGC, which was intracerebrally injected into the 6-d...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000081744

    authors: Lee YR,Huang KJ,Lei HY,Chen SH,Lin YS,Yeh TM,Liu HS

    更新日期:2005-01-01 00:00:00

  • Early alterations in the morphology of cytomegalovirus-infected human fibroblasts.

    abstract::Cytomegalovirus-infected human fibroblasts developed large numbers of fine microvilli within 90 min after addition of virus. The structure of these microvilli gradually changed as the infection proceeded, although the infected cells were easily detected by their large number of processes. These processes may be involv...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149058

    authors: Garnett HM

    更新日期:1979-01-01 00:00:00

  • Selected amino acid change encoding Epstein-Barr virus-specific T cell epitope of the LMP2A gene in Japanese nasal NK/T cell lymphoma patients.

    abstract::Nasal natural killer (NK)/T cell lymphoma is a peculiar lymphoma with a unique immunophenotype. Etiologically, in 1990, the authors first demonstrated the presence of Epstein-Barr virus (EBV) genomes and their products in this lymphoma. EBV-specific cytotoxic T lymphocytes (CTL) are very important in controlling the l...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000106462

    authors: Nagamine M,Kishibe K,Takahara M,Nagato T,Ishii H,Bandoh N,Ogino T,Harabuchi Y

    更新日期:2007-01-01 00:00:00

  • Shedding of virus-free MMTV gp52 by nonproducer mammary tumor cells: a source of tumor marker in the presence and absence of dexamethasone.

    abstract::The envelope glycoprotein of the mouse mammary tumor virus (MMTV gp52), a useful plasma marker for mammary tumors in mice, was measured in extracellular medium by radioimmunoassay to quantitatively compare gp52 levels released by a C3H MMTV producer and its subcloned B9 nonproducer cell line. Experiments analyzed leve...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000150081

    authors: Ritzi EM,Walthall EB

    更新日期:1989-01-01 00:00:00

  • Coxsackievirus B3 induces apoptosis in the early phase of murine myocarditis: a comparative analysis of cardiovirulent and noncardiovirulent strains.

    abstract:OBJECTIVE:To investigate the relationship between enteroviral infection and myocardial tissue apoptosis during the development of viral myocarditis in a murine model. METHODS:C3H/HeJ mice were inoculated with two strains of coxsackievirus B3, specifically CVB3 (cardiovirulent Nancy strain) and CVB3/0 (noncardiovirulen...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000071453

    authors: Joo CH,Hong HN,Kim EO,Im JO,Yoon SY,Ye JS,Moon MS,Kim D,Lee H,Kim YK

    更新日期:2003-01-01 00:00:00

  • The two capsid proteins of maize rayado fino virus contain common peptide sequences.

    abstract::Virions of maize rayado fino virus (MRFV) were purified and two major capsid proteins (ca. Mr 29,000 and 22,000) were resolved by SDS-PAGE. When the two major capsid proteins were isolated from gels and compared by one-dimensional peptide mapping after digestion with Staphylococcus aureus V-8 protease, indistinguishab...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149664

    authors: Falk BW,Tsai JH

    更新日期:1986-01-01 00:00:00

  • Polyploidization of Epstein-Barr virus (EBV)-carrying lymphoma lines decreases the inducibility of EBV-determined early antigen following P3HR-1 virus superinfection or iododeoxyuridine treatment.

    abstract::Four tetraploid cell lines were derived from the Epstein-Barr virus (EBV)-carrying, nonproducer Burkitt lymphoma line Raji after colcemide treatment. EBV early antigen (EA) was induced by P3HR-1 virus superinfection and by iododeoxyuridine treatment. Inducibility was reduced in all tetraploid lines compared to the nea...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000148973

    authors: Shapiro IM,Andersson-Anvret M,Klein G

    更新日期:1978-01-01 00:00:00

  • Progressive changes in hepatoma cells stably transfected with hepatitis B virus X gene.

    abstract:OBJECTIVE:The aim of this study is to investigate the molecular mechanism of hepatocellular carcinoma (HCC) development induced by hepatitis B virus X protein (HBx). METHODS:We previously established a H7402-X cell line that constitutively expresses HBx protein. In the present study, H7402-X gene expression profiles a...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000120289

    authors: Ye L,Dong N,Wang Q,Xu Z,Cai N,Wang H,Zhang X

    更新日期:2008-01-01 00:00:00

  • Contrasting characteristics of Marek's disease herpesvirus isolated from chickens with and without avian leukosis virus infection.

    abstract::Marek's disease herpesvirus (MDHV) isolated from chickens free of naturally occurring avian leukosis virus (ALV) infection produced characteristic foci in both chicken embryo fibroblast (CEF) and chicken kidney cell (CKC) cultures. MDHV-A, which was extracted from the feather follicle epithelium of chickens naturally ...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000148963

    authors: Campbell WF,Kufe DW,Peters WP,Spiegelman S,Frankel JW

    更新日期:1978-01-01 00:00:00

  • The Genotyping Resistance Profile of the Pol Gene Detected in Blood of Newly Diagnosed HIV-Positive Men Is Durably Archived in the Gut Whatever the Time of Initiation of cART.

    abstract:OBJECTIVE:To evaluate the mutational patterns on the pol gene of the main HIV-1 strain archived in cell genome of 10 chronically infected men according to their clinical and therapeutic history. The genotyping resistance profiles were compared between the first blood plasma available at the time of HIV diagnosis and re...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000472716

    authors: Depincé AE,Dussert E,Vergnon-Miszczycha D,Keita A,Pillet S,Botelho-Nevers E,Frésard A,Gagneux-Brunon A,Lucht FR,Roblin X,Pozzetto B,Paul S,Bourlet T

    更新日期:2016-01-01 00:00:00