Abstract:
:The envelope glycoprotein of the mouse mammary tumor virus (MMTV gp52), a useful plasma marker for mammary tumors in mice, was measured in extracellular medium by radioimmunoassay to quantitatively compare gp52 levels released by a C3H MMTV producer and its subcloned B9 nonproducer cell line. Experiments analyzed levels of viral gp52 released from nonproducer cells both in the presence and absence of dexamethasone (DXS) to compare levels with those of producer cells and to assess quantitative changes in nonproducer release of both soluble and virion-associated gp52. Basal gp52 levels of producer cells were 2.4- to 7.0-fold higher than nonproducer cells. Very little virion-associated gp52 was detected in nonproducer cultures, whereas soluble gp52 was abundant and easily detectable in both cultures. DXS stimulated virus production in B9 cultures, converting nonproducer cells into producers. However, under identical conditions of DXS stimulation, B9 cells never reached the same elevated levels of gp52 as producer cells (90 ng/10(6) cells versus 216 ng/10(6) cells). Significant soluble gp52 release by B9 cells in the absence of DXS and enhancement in the presence of DXS argues that nonproducer cells can influence extracellular levels of viral antigen. By analogy to in vivo studies, results suggest that nonproducer cells in a tumor might also contribute to the pool of gp52 which serves as a systemic plasma marker for tumor.
journal_name
Intervirologyjournal_title
Intervirologyauthors
Ritzi EM,Walthall EBdoi
10.1159/000150081subject
Has Abstractpub_date
1989-01-01 00:00:00pages
102-10issue
2eissn
0300-5526issn
1423-0100journal_volume
30pub_type
杂志文章相关文献
INTERVIROLOGY文献大全abstract::The PLC/PRF/5 human hepatoma cell line producing hepatitis B surface antigen (HBsAg) was studied to determine whether infectious hepatitis B virus (HBV) was also being produced. 2 chimpanzees with no previous exposure to HBV and no serologic markers of past or active HBV infection were inoculated intravenously with 50...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149217
更新日期:1981-01-01 00:00:00
abstract::A whitefly-transmitted virus from Venezuela, potato yellow mosaic virus, has been propagated, isolated and characterized as a member of the Geminivirus group. The virus was transmitted to several species of Nicotiana and to Petunia hybrida by mechanical inoculation, and to potato and tomato plants by grafting. Purifie...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150042
更新日期:1988-01-01 00:00:00
abstract::Human papillomavirus (HPV) DNA was isolated from a clinically diagnosed flat wart and proved to be related to HPV2. The isolate showed 55% cross-hybridization with HPV2a. A physical map of restriction enzyme cleavage sites differed completely from those of HPV2a and HPV2b. The new HPV2 subtype, which will be classifie...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149548
更新日期:1984-01-01 00:00:00
abstract::Virions of maize rayado fino virus (MRFV) were purified and two major capsid proteins (ca. Mr 29,000 and 22,000) were resolved by SDS-PAGE. When the two major capsid proteins were isolated from gels and compared by one-dimensional peptide mapping after digestion with Staphylococcus aureus V-8 protease, indistinguishab...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149664
更新日期:1986-01-01 00:00:00
abstract::Poliovirus infection of either undifferentiated or differentiated U937 cells produced a decrease in the percentage of cells positive for the surface expression of the CD4, CD11c, CD14, or 8E11 antigens. The number of 4F2 surface molecules per cell increased in infected normal U937 cells, but was unaffected in differen...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150194
更新日期:1991-01-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) latency was established in human embryo lung (HEL) cells by treatment with (E)-5-(2'-bromovinyl)-2'-deoxyuridine and human leukocyte interferon and subsequent temperature increase (from 37 degrees to 40.5 degrees). This in vitro system was used to study reactivation after temperatur...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149668
更新日期:1986-01-01 00:00:00
abstract::In this study, we analyzed the in vitro expression, potency and longevity of immune responses induced in a Balb/c mouse model by a synthetic HIV-1 GAG gene exhibiting a codon usage that was adapted to that of highly expressed mammalian genes (syngag). In contrast to a vector containing the wild-type (wt) GAG gene, the...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000067919
更新日期:2002-01-01 00:00:00
abstract:OBJECTIVES:To express E1 glycoprotein of rubella virus (RuV) strain JR23 in yeast and develop a diagnostic assay using expressed E1 protein as coating antigen in comparison with other diagnostic assays. METHODS:cDNA of E1 open reading frame of RuV was PCR amplified using plasmid pMD18-T-E1 as template and cloned into ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000085101
更新日期:2005-01-01 00:00:00
abstract::Scrapie brain homogenate was mixed with mouse peritoneal macrophages in vitro. After 2 h of incubation at 37 degrees, a portion of the scrapie infectivity was associated with macrophages. In contrast, very little infectivity was associated with kidney cells that had been exposed to scrapie brain homogenate. After incu...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149241
更新日期:1981-01-01 00:00:00
abstract::The results of this study show that the synthesis of the inner mitochondrial membrane phospholipid, cardiolipin, is markedly inhibited following infection of human embryonic lung or hamster embryo fibroblast cells with herpes simplex virus type 1 or 2. The synthesis of other phospholipids, i.e., phosphatidylcholine, p...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149969
更新日期:1974-01-01 00:00:00
abstract::The aim of the study was to determine the prevalence of drug resistance of HIV-1 in pediatric patients from Istanbul, Turkey. Genotypic drug resistance testing revealed transmission of drug resistance from mother to child in 20%. Due to rising numbers of children with HIV-1, baseline resistance testing is recommended ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000362335
更新日期:2014-01-01 00:00:00
abstract::A seroepidemiological survey of the human syncytial (foamy) virus was done by means of an indirect immunofluorescence test on 1,717 sera from nine different Pacific island territories. The specificity of the reaction was verified by neutralization tests. The study indicated that the virus is ubiquitous in this part of...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149112
更新日期:1980-01-01 00:00:00
abstract:BACKGROUND/AIMS:The present study was launched to systematically characterize the molecular genotypes and quantitative serological biomarkers during the natural course of chronic hepatitis B (CHB) infection in a patient population from Chongqing, China. METHODS:A total of 140 patients were enrolled and grouped accordi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000323524
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVES:Single-nucleotide polymorphisms (SNPs) near the interleukin (IL) 28B gene encoding a type III interferon (IFN-λ) are the most important genetic predictors of treatment response to hepatitis C virus (HCV). This retrospective study was undertaken to determine any association between IL28B SNPs and the developm...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000357326
更新日期:2014-01-01 00:00:00
abstract::The 20-nm noninfectious empty envelope particles carrying the hepatitis B surface antigen are secreted in large excess from hepatocytes during a hepatitis B infection. Similar particles produced in cell lines or yeast are an efficient vaccine against hepatitis B virus. We have analyzed the assembly of 20-nm particles ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150410
更新日期:1995-01-01 00:00:00
abstract::The phosphorylation state of H5R protein was investigated by two-dimensional gel electrophoresis of proteins of BSC-40 cells infected at 32 degrees or 39.5 degrees with vaccinia virus ts mutants of the viral B1R or F10L protein kinase genes. A temperature-dependent increase of underphosphorylated H5R protein (pI 6.8) ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000025025
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND:Although human leukocyte antigens (HLA) have been shown in association with the outcomes of hepatitis C virus (HCV) infection among different ethnic groups, such studies remain absent in China, where the HCV prevalence is higher than the global average. METHODS:In this study, 426 HCV-infected and 709 uninfe...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000369209
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Many efforts are currently focused on functional treatment of the hepatitis B virus (HBV). This can be done by suppressing the secretion of HBV surface antigen (HBsAg). Scientific communities are very interested in natural products in that respect. OBJECTIVE:Use of root extract of Havachoobe (Onosma dichroa...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000512140
更新日期:2020-12-15 00:00:00
abstract:OBJECTIVES:In the present study, we aimed to determine whether signal-to-cutoff (S/Co) ratios of reactive anti-HCV samples could be used as a basis for avoiding the need for supplemental testing in our study population. METHODS:We analyzed 901 anti-HCV-positive sera from 8 institutions in China. The Ortho VITROS anti-...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000441474
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:The aim was to identify the codon usage bias between the newly identified duck plague virus (DPV) UL35 gene (GenBank accession No. EF643558) and the UL35-like genes of 27 other reference herpesviruses. METHODS:A comparative analysis of the codon usage bias of the 28 herpesviruses was performed by using the C...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000231992
更新日期:2009-01-01 00:00:00
abstract::A singly enveloped nuclear polyhedrosis virus (SNPV) of the white-marked tussock moth, Orgyia leucostigma, was successfully grown in four continuous cell lines developed from minced neonate larvae of this insect. Level of infection in two cell lines, IPRI-OL-12 and IPRI-OL-13, was 65-90%, but in the other two, IPRI-OL...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149502
更新日期:1984-01-01 00:00:00
abstract::In this study, 574 stool samples from children with gastroenteritis were obtained from different hospitals in Costa Rica, Honduras, Nicaragua and the Dominican Republic during 2005-2006. Diarrhea stool samples were analyzed for rotavirus by ELISA and typed by the RT-PCR-based method. Unusual strains were detected: G1P...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000317288
更新日期:2010-01-01 00:00:00
abstract::Previous reports documented the recovery of a DNA virus from a patient with posttransfusion non-A-G hepatitis and named TT virus (TTV). Although the virus was initially detected as a causative agent of hepatitis, there is doubt about its pathogenicity. The aim of this study was to clarify the relationship between TTV ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000050060
更新日期:2001-01-01 00:00:00
abstract::The genetic relationship between two enteroviruses causing the acute hemorrhagic conjunctivitis (AHC) syndrome was examined by the RNA-RNA hybridization technique. Nucleotide sequence homology between the J670/71 strain of enterovirus type 70 (EV70) and the EH 24/70 strain of Singapore epidemic conjunctivitis (SEC) vi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149539
更新日期:1984-01-01 00:00:00
abstract::The complete nucleotide sequence of rice dwarf phytoreovirus genome segment 2 (S2) was determined to be 3,512 nucleotides long with one open reading frame initiating at nucleotide 15 and terminating at nucleotide 3363. The encoded polypeptide was predicted to have 1,116 residues with a relative molecular weight of 123...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150348
更新日期:1994-01-01 00:00:00
abstract:OBJECTIVE:Giant viruses of the Megavirales order have been neglected in the literature because they are removed from samples during viral purification for viral metagenomic studies. Isolation via amoeba coculture has low efficiency and is extremely time-consuming. Thus, our objective was to improve Megavirales detectio...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000354566
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:Cervical cancer is the third most common malignancy in females. Since the human papillomavirus (HPV) genotype can vary geographically, it is essential to define its genotypic distribution before establishing health care policies and vaccination programs in any given area. The aim of this study was to determin...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000477848
更新日期:2017-01-01 00:00:00
abstract::Acyclovir inhibits herpes simplex virus (HSV) infections better in human fibroblasts than in green monkey kidney cells. Thymidine and acyclovir compete for the active site of HSV thymidine kinase. Large intracellular pools of thymidine found by high-performance liquid chromatography (HPLC) seemed to be responsible for...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149373
更新日期:1983-01-01 00:00:00
abstract::Biochemical studies on human coronavirus, strain 229E, indicate that the RNA is present in the virion in association with protein as a ribonucleoprotein (RNP) complex. Morphological studies have revealed that this nucleocapsid is probably a continuous helical RNP. ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149474
更新日期:1975-01-01 00:00:00
abstract::Five distinct antigenic sites recognized by cytotoxic T lymphocytes in H-2b mice have been identified on simian virus 40 (SV40) T antigen. Cocultivation of SV40-transformed cells with cytotoxic T lymphocyte (CTL) clones specific for epitopes I-IV results in the selection of variants which show a loss of either epitope...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150154
更新日期:1990-01-01 00:00:00