Abstract:
:Virions of maize rayado fino virus (MRFV) were purified and two major capsid proteins (ca. Mr 29,000 and 22,000) were resolved by SDS-PAGE. When the two major capsid proteins were isolated from gels and compared by one-dimensional peptide mapping after digestion with Staphylococcus aureus V-8 protease, indistinguishable peptide maps were obtained, suggesting that these two proteins contain common peptide sequences. Some preparations also showed minor protein components that were intermediate between the Mr 22,000 and Mr 29,000 capsid proteins. One of the minor proteins, ca. Mr 27,000, gave a peptide map indistinguishable from the major capsid proteins. In vitro ageing of partially purified preparations or virion treatment with proteolytic enzymes failed to show conversion of the Mr 29,000 protein to a Mr 22,000. Protease inhibitors added to the buffers used for virion purification did not affect the apparent 1:3 ratio of 29,000 to 22,000 proteins in the purified preparations.
journal_name
Intervirologyjournal_title
Intervirologyauthors
Falk BW,Tsai JHdoi
10.1159/000149664subject
Has Abstractpub_date
1986-01-01 00:00:00pages
111-6issue
2eissn
0300-5526issn
1423-0100journal_volume
25pub_type
杂志文章相关文献
INTERVIROLOGY文献大全abstract::A SmaI map of class I defective DNA of herpes simplex virus type 1 (HSV1) was constructed using a set of deletion hybrid phages. Four SmaI fragments on the defective DNA had a variability in length common among 15 HSV1 isolates: the 1.45 kilobase (kb) fragment located within the BamHI-Z (map coordinates 0.936-0.949) f...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149596
更新日期:1985-01-01 00:00:00
abstract:BACKGROUND:We have recently demonstrated that an oligodeoxynucleotide (ODN) can enter HIV particles and form a local hybrid at the highly conserved polypurine tract (PPT), the target site of the ODN. This hybrid is recognized by the retrovirus-specific RNase H, which is a virion-associated enzyme. It cleaves the RNA at...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000324544
更新日期:2012-01-01 00:00:00
abstract::The kinetics of Aleutian disease virus (ADV) replication in mink lymphocytes was followed by the analysis of virus-specific antigens, infectious virus, and viral DNA. Stimulation with pokeweed mitogen (PWM; 20 micrograms/ml) increased the synthesis of ADV DNA in cultivated cells of the B-cell fraction. Maximum virus t...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149676
更新日期:1986-01-01 00:00:00
abstract:OBJECTIVE:Human Salivirus (SalV) has been associated with gastroenteritis on all continents. METHODS:This paper presents the real-time RT-PCR assay for the detection of SalV in clinical fecal samples collected from 192 hospitalized children with acute gastroenteritis in Piedmont, Italy. RESULTS:The most commonly dete...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000490568
更新日期:2018-01-01 00:00:00
abstract::The interaction of Autographa californica nuclear polyhedrosis virus with two nonpermissive cell lines was investigated. The insect cell line, CP 169, and the Chinese hamster cell line, CHO-K1, were able to adsorb and engulf virus particles, but there was no evidence for viral replication in these cells based on virus...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149522
更新日期:1984-01-01 00:00:00
abstract::Despite frequent statements to the contrary, there are good animal models for AIDS. In this review, we summarize the properties of one of the most useful animal models: infection of rhesus monkeys with simian immunodeficiency virus (SIV). The SIVs are an extensive group of HIV-related lentiviruses of nonhuman primates...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000150108
更新日期:1989-01-01 00:00:00
abstract::Despite the existence for some time of effective prophylactic vaccines, hepatitis B virus (HBV) infection remains an important global concern. Improvements on existing vaccines could be beneficial, especially in situations where it is desirable or necessary to induce protective immunity more rapidly or with fewer dose...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000089375
更新日期:2006-01-01 00:00:00
abstract::Despite the significant medical advances which have taken place in the last decades, acute diarrhoea cases remain a public health issue of major significance, with gastroenteritis agents being associated with severe symptoms in adults and high morbidity in infants and children. Regarding rotaviruses, while children ar...
journal_title:Intervirology
pub_type: 新闻
doi:10.1159/000502007
更新日期:2019-01-01 00:00:00
abstract::We report a case in which sustained viral response was achieved after switching treatment from pegylated interferon (PEG-IFN) α-2b to α-2a and ribavirin (RBV) in patients with recurrence of hepatitis C virus (HCV) infection after living donor liver transplantation. The patient was a 62-year-old man with liver cirrhosi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000328661
更新日期:2012-01-01 00:00:00
abstract::The phosphorylation state of H5R protein was investigated by two-dimensional gel electrophoresis of proteins of BSC-40 cells infected at 32 degrees or 39.5 degrees with vaccinia virus ts mutants of the viral B1R or F10L protein kinase genes. A temperature-dependent increase of underphosphorylated H5R protein (pI 6.8) ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000025025
更新日期:2000-01-01 00:00:00
abstract:OBJECTIVES:To characterize morphological, physicochemical and genomic features of a novel virulent coliphage which was isolated from an engineered Escherichia coli culture and termed engineered E. coli phage (EEP). METHODS AND RESULTS:Electron microscopy revealed that EEP has an icosahedral head (62 nm in diameter) an...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000299063
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVE:Because the determination of the stage of fibrosis depends on rather subjective judgment, more objective parameters are needed. In this study, we followed the long-term outcome, with monitoring of platelet counts, in patients with chronic hepatitis C or liver cirrhosis (LC) who had undergone interferon (IFN) ...
journal_title:Intervirology
pub_type: 临床试验,杂志文章
doi:10.1159/000073209
更新日期:2003-01-01 00:00:00
abstract::In order to assess the risk of transmission of viral diseases during floods, the viral burden in flooded areas of the city of Dresden (Germany) in August 2002 was investigated. Water samples were collected from 9 sampling sites and tested for the presence of 11 enteric viral pathogens. As a control, water samples from...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000095157
更新日期:2006-01-01 00:00:00
abstract::The actinomycin-D(AMD)-sensitive step of replication of three plant viruses - cowpea chlorotic mottle virus (CCMV), cowpea mosaic virus, and the cowpea strain of tobacco mosaic virus - in cowpea was specific for each virus. Different combinations of the viruses were examined in which leaves were inoculated with one vi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149524
更新日期:1984-01-01 00:00:00
abstract:OBJECTIVE:Genome-wide sequences of hepatitis B virus strain associated with type B fulminant hepatitis have not been compared with those of acute self-limited hepatitis. We carried out full-length sequencing analysis of viral strains derived from patients with type B acute liver injury. METHODS:Nine acute self-limited...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000110651
更新日期:2007-01-01 00:00:00
abstract::Cytomegalovirus infection of Brown Norway rats was studied after intraperitoneal or subcutaneous inoculation of virus. No clinical illness was apparent during the 1st month postinfection (p.i.). Low titers of virus were detected in many organs at day 4 p.i. for the intraperitoneally inoculated animals and at day 11 p....
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149612
更新日期:1985-01-01 00:00:00
abstract:BACKGROUND/AIMS:The nucleo-cytoplasmic large DNA viruses (NCLDV) constitute an apparently monophyletic group that consists of 6 families of viruses infecting a broad variety of eukaryotes. A comprehensive genome comparison and maximum-likelihood reconstruction of NCLDV evolution reveal a set of approximately 50 conserv...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000312913
更新日期:2010-01-01 00:00:00
abstract::Solubilized envelope antigens (glycoproteins) of Sendai virus were purified in a DEAE-Bio-Gel A column. Monospecific antisera were prepared against the antigens, designated HN and F glycoproteins. These glycoproteins are antigenically distinct. Immunodiffusion analyses of the envelope antigens of three representative ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149462
更新日期:1975-01-01 00:00:00
abstract::There are two major requirements for potyvirus taxonomy; group-specific criteria and criteria which discriminate between distinct viruses and strains. This review discusses the relative merits in potyvirus taxonomy of molecular parameters, such as gene sequence data, nucleic acid hybridization, coat protein sequence d...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000150211
更新日期:1991-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the distribution of hepatitis C virus (HCV) genotype and the possible association between genotype and HCV-associated liver disease in Indonesia. METHODS:32 anti-HCV-positive asymptomatic carriers (AC), 55 chronic hepatitis (CH), 41 liver cirrhosis (LC), and 35 hepato...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000205515
更新日期:2008-01-01 00:00:00
abstract::The PLC/PRF/5 human hepatoma cell line producing hepatitis B surface antigen (HBsAg) was studied to determine whether infectious hepatitis B virus (HBV) was also being produced. 2 chimpanzees with no previous exposure to HBV and no serologic markers of past or active HBV infection were inoculated intravenously with 50...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149217
更新日期:1981-01-01 00:00:00
abstract::Prostaglandins exhibit antiviral activity against a wide variety of RNA and DNA viruses. In the present report, we describe the effect of cyclopentenone prostaglandin A(1) (PGA(1)) on Mayaro virus replication in Vero cells. Virus yield was significantly reduced at nontoxic concentrations which did not suppress DNA, RN...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000050069
更新日期:2001-01-01 00:00:00
abstract::We investigated persistent infection of Epstein-Barr virus (EBV)-transformed lymphoblastoid cells with dengue virus using a transmission electron microscope. Most of the infected cells kept an intact morphology with only a few virus particles in the cytoplasm, but without any indication of active viral replication. So...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000050030
更新日期:2001-01-01 00:00:00
abstract::Rotavirus is the most common cause of severe diarrhoea worldwide, affecting over 125 million young children every year in developing countries. The present study is a part of ongoing childhood diarrhoeal surveillance to determine the strain diversity of rotaviruses prevalent in Andaman and Nicobar Islands. From Octobe...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000342219
更新日期:2013-01-01 00:00:00
abstract::Virus-associated DNA polymerase activity has recently been proposed for the detection of human cytomegalovirus (HCMV) in urine, a method that should allow rapid and quantitative determination of the viral load. In this report, the virus-associated DNA polymerase activity recovered from the urine of a group of patients...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150530
更新日期:1996-01-01 00:00:00
abstract::Marituba virus was purified by rate-zonal sedimentation and equilibrium density centrifugation in sucrose gradients. The buoyant density of virus particles was 1.19 g/ml. Purified virus was dissociated and its proteins were analyzed by SDS-PAGE. Four virus polypeptides were identified and designated L, G1, G2 and N. T...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149383
更新日期:1983-01-01 00:00:00
abstract::Two DNA-negative temperature-sensitive mutants of human cytomegalovirus strain AD169 were shown to be deficient in induction of several prominent viral polypeptides when grown in human foreskin fibroblasts under nonpermissive conditions. The use of a monospecific polyclonal antiserum allowed recognition of a further d...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150034
更新日期:1988-01-01 00:00:00
abstract:OBJECTIVE:Characterization of the phylogeny and diversity of human respiratory syncytial virus (HRSV) genotype ON1 that occurred during its early evolution (within the first 3.5 years since the detection of the first ON1 strains). ON1 strains have a 72-nucleotide-long in-frame duplication within the second hypervariabl...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000382018
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:Pandemic viral infections as emerging infectious diseases are of a great global concern. However, for some viruses, particular strains are endemic to specific areas and can be genetically distinguished from strains in other regions. In contrast, for some other viruses, genetically similar strains can spread a...
journal_title:Intervirology
pub_type: 杂志文章,meta分析
doi:10.1159/000320967
更新日期:2011-01-01 00:00:00
abstract::Intrauterine viral infections show a wide range of fetal involvement with variable fetal outcome. The histopathologic changes of the placenta during viral infection show a wide spectrum. The most active processes are proliferative with a variety of cells involved. Vascular changes, including inflammatory and obliterat...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000024940
更新日期:1998-01-01 00:00:00