Abstract:
:The PLC/PRF/5 human hepatoma cell line producing hepatitis B surface antigen (HBsAg) was studied to determine whether infectious hepatitis B virus (HBV) was also being produced. 2 chimpanzees with no previous exposure to HBV and no serologic markers of past or active HBV infection were inoculated intravenously with 50 ml of either tissue culture supernatant fluid (357 ng/ml HBsAg) or a suspension of cells disrupted by repeated freeze-thaw cycles (57 ng/ml HBsAg). No evidence of HBV infection was detected in either chimpanzee during 6 months of evaluation. This study suggests that the expression of a portion of the HBV genome, when a portion or all of that genome has been incorporated into a host cell, can result in the production of HBsAg without infectious HBV. If it becomes possible to produce a similar expression of this portion of the genome by itself in nonmalignant cells, HBsAg without HBV may be produced in vitro for use in hepatitis B vaccines.
journal_name
Intervirologyjournal_title
Intervirologyauthors
Tabor E,Copeland JA,Mann GF,Howard CR,Skelly J,Snoy P,Zuckerman AJ,Gerety RJdoi
10.1159/000149217subject
Has Abstractpub_date
1981-01-01 00:00:00pages
82-6issue
2eissn
0300-5526issn
1423-0100journal_volume
15pub_type
杂志文章相关文献
INTERVIROLOGY文献大全abstract::Dengue and chikungunya fevers are transmitted by the common mosquito vector Aedes and malaria by Anopheles. Concurrent infections are reported due to co-circulation of these pathogens, especially in endemic regions. We report a rare case of triple infection with 3 arthropod-borne pathogens (Plasmodium vivax and the de...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000479430
更新日期:2017-01-01 00:00:00
abstract::Intrauterine viral infections show a wide range of fetal involvement with variable fetal outcome. The histopathologic changes of the placenta during viral infection show a wide spectrum. The most active processes are proliferative with a variety of cells involved. Vascular changes, including inflammatory and obliterat...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000024940
更新日期:1998-01-01 00:00:00
abstract::Human papillomavirus (HPV) DNA was isolated from a clinically diagnosed flat wart and proved to be related to HPV2. The isolate showed 55% cross-hybridization with HPV2a. A physical map of restriction enzyme cleavage sites differed completely from those of HPV2a and HPV2b. The new HPV2 subtype, which will be classifie...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149548
更新日期:1984-01-01 00:00:00
abstract::Marituba virus was purified by rate-zonal sedimentation and equilibrium density centrifugation in sucrose gradients. The buoyant density of virus particles was 1.19 g/ml. Purified virus was dissociated and its proteins were analyzed by SDS-PAGE. Four virus polypeptides were identified and designated L, G1, G2 and N. T...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149383
更新日期:1983-01-01 00:00:00
abstract::This review briefly summarises the recent knowledge about hantavirus infections and raises particular problems in hantavirus research that need further investigation. The following questions are addressed: (i) are hantaviruses distributed worldwide and what leads to new outbreaks, (ii) what is known about hantavirus e...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000067924
更新日期:2002-01-01 00:00:00
abstract::Infection with hepatitis B virus (HBV) continues to be a major problem in the human population. There remains a specific requirement for HBV vaccines capable of circumventing the non-responder/inadequate responder status of some vaccinees. Hepacare has been primarily developed to (1) improve anti-SHBs antibody titres ...
journal_title:Intervirology
pub_type: 临床试验,杂志文章
doi:10.1159/000050036
更新日期:2001-01-01 00:00:00
abstract::SDS-polyacrylamide slab gel analysis of selected primate and nonprimate adenoviruses has shown these viruses to differ significantly at the polypeptide molecular level. It follows that data derived from study of a single adenovirus serotype are unique to that serotype and are not representative of the adenovirus group...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149001
更新日期:1978-01-01 00:00:00
abstract::We examined influenza virus strains of the subtype H3N2 from outbreaks of respiratory diseases in swine herds in Germany. Four different clusters can be distinguished when comparing parts of the HA1 gene from porcine H3N2 isolates analyzed between 1982 and 2001. Comparison between these clusters reveals a bp homology ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000077829
更新日期:2004-01-01 00:00:00
abstract::We have analyzed viral DNA patterns, obtained following digestion of six different infectious bovine rhinotracheitis isolates, using a panel of restriction enzymes. Several differences in profiles were observed, particularly for a Canadian viral strain which presumably contained a genome that was larger than the other...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150192
更新日期:1991-01-01 00:00:00
abstract::Hybrid recombinant plasmids were constructed; they were composed of the herpes simplex virus type 2 (HSV2) thymidine kinase (tk) gene and DNA sequences of HSV2 that have been reported to induce morphological and/or oncogenic transformation of rodent cells in culture. Several plasmids were made in two versions, with or...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149644
更新日期:1985-01-01 00:00:00
abstract:OBJECTIVES:The potential risk of accidental infection by hantaviruses in a clinical or research laboratory necessitates special precautionary measures. A biosafety program must address handling and disposal of infectious materials as well as appropriate virus inactivation or depletion procedures to permit necessary fur...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000084603
更新日期:2005-01-01 00:00:00
abstract::Poliovirus infection of either undifferentiated or differentiated U937 cells produced a decrease in the percentage of cells positive for the surface expression of the CD4, CD11c, CD14, or 8E11 antigens. The number of 4F2 surface molecules per cell increased in infected normal U937 cells, but was unaffected in differen...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150194
更新日期:1991-01-01 00:00:00
abstract:OBJECTIVE:The specific effect of rna interference on the replication of porcine hemagglutinating encephalomyelitis virus (phe-cov) was explored. METHODS:Four species of small interfering RNA (siRNA), targeting different regions of the PHE-CoV spike glycoprotein and replicase polyprotein genes, were prepared by in vitr...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000323523
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVES:To explore the host transcription factor E2F-1 and/or any mechanisms that may support viral entry and its effect on the HSV-1 production in anti-CD3-activated Jurkat cells. METHODS:The expressions of ICP4, HVEM and E2F-1 were studied using reverse transcription-PCR and Western blot. HSV-1 production was det...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000437264
更新日期:2015-01-01 00:00:00
abstract::The effect of caffeine on cytomegalovirus (CMV) replication in human embryo lung (HEL) and human embryo kidney (HEK) cells was studied. In HEL cultures, nonirradiated CMV yielded up to 6.7 times more plaques in caffeine-treated cultures than in control cultures. Virus growth curves exhibited different profiles, depend...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000148987
更新日期:1978-01-01 00:00:00
abstract:BACKGROUND/AIMS:Adsorption and kinetic parameters, latent period, burst size and burst time, are characteristics of phage/host systems and can be affected by several environmental factors. As only few studies have focused on temperate dairy phages, we characterized these parameters on temperate Lactobacillus paracasei ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000369207
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:Some patients with chronic hepatitis C virus (HCV) infection fail to achieve complete early virologic response (EVR) despite a marked decrease in HCV RNA at 4 weeks. We investigated the characteristics and final treatment outcomes of this patient subpopulation. METHODS:A total of 516 patients with HCV genoty...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000362721
更新日期:2014-01-01 00:00:00
abstract::A whitefly-transmitted virus from Venezuela, potato yellow mosaic virus, has been propagated, isolated and characterized as a member of the Geminivirus group. The virus was transmitted to several species of Nicotiana and to Petunia hybrida by mechanical inoculation, and to potato and tomato plants by grafting. Purifie...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150042
更新日期:1988-01-01 00:00:00
abstract::The aim of the study was to determine the prevalence of drug resistance of HIV-1 in pediatric patients from Istanbul, Turkey. Genotypic drug resistance testing revealed transmission of drug resistance from mother to child in 20%. Due to rising numbers of children with HIV-1, baseline resistance testing is recommended ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000362335
更新日期:2014-01-01 00:00:00
abstract::The actinomycin-D(AMD)-sensitive step of replication of three plant viruses - cowpea chlorotic mottle virus (CCMV), cowpea mosaic virus, and the cowpea strain of tobacco mosaic virus - in cowpea was specific for each virus. Different combinations of the viruses were examined in which leaves were inoculated with one vi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149524
更新日期:1984-01-01 00:00:00
abstract::Immunopathologic mechanisms leading to liver tissue injury in hepatitis caused by hepatitis A virus (HAV) were studied in an autologous in vitro model. Data show virus-specific killing by liver-infiltrating T lymphocytes in man and support the hypothesis that hepatocellular damage as well as efficient elimination of v...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150304
更新日期:1993-01-01 00:00:00
abstract:OBJECTIVE:The aim was to identify the codon usage bias between the newly identified duck plague virus (DPV) UL35 gene (GenBank accession No. EF643558) and the UL35-like genes of 27 other reference herpesviruses. METHODS:A comparative analysis of the codon usage bias of the 28 herpesviruses was performed by using the C...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000231992
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND/AIMS:In Zimbabwe, cervical cancer is screened through cytology and visual inspection with acetic acid and cervicography (VIAC). The effectiveness of these methods can be increased if complemented by a human papillomavirus DNA detection tool since most cervical cancer cases are caused by persistent infection ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000502206
更新日期:2019-01-01 00:00:00
abstract::The use of glutaraldehyde (GA)-treated chicken red blood cells (RBC) for purification of Sendai virions by adsorption and elution was found to be advantageous over the use of untreated RBC. Biologic and enzymatic activities and structural components of the purified virions were compared with those of the original viri...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000148872
更新日期:1977-01-01 00:00:00
abstract::The use of bleach (hypochlorite) as a disinfectant for drug injection equipment in the intravenous-drug-using population was recommended early in the HIV-1/AIDS epidemic. Epidemiological studies have challenged the use of bleach as an effective measure to prevent HIV-1 transmission. However, in vitro HIV-1 coculture s...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000025015
更新日期:2000-01-01 00:00:00
abstract::Coinfections with hepatitis B virus (HBV) and HIV are very frequent. Although HBV is a DNA virus, it replicates via reverse transcription like HIV. Structural similarities between the enzymatic pocket of the HBV DNA polymerase and HIV-1 reverse transcriptase are the basis that certain drugs inhibit both enzymes and th...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000360942
更新日期:2014-01-01 00:00:00
abstract::A serologic comparison was performed by immune electron microscopy between hepatitis A antigen (related to the MS-1 strain of hepatitis A virus) and a viral isolate, CR69(076), from the stool of a patient in Costa Rica with viral hepatitis. CR69(076) was not antigenically related to hepatitis A antigen but was related...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149486
更新日期:1975-01-01 00:00:00
abstract::Virus-associated DNA polymerase activity has recently been proposed for the detection of human cytomegalovirus (HCMV) in urine, a method that should allow rapid and quantitative determination of the viral load. In this report, the virus-associated DNA polymerase activity recovered from the urine of a group of patients...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150530
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND/AIMS:The present study was launched to systematically characterize the molecular genotypes and quantitative serological biomarkers during the natural course of chronic hepatitis B (CHB) infection in a patient population from Chongqing, China. METHODS:A total of 140 patients were enrolled and grouped accordi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000323524
更新日期:2012-01-01 00:00:00
abstract::Rauscher murine leukemia virus (RLV) infection of adult mice results in splenomegaly that is proportional to the virus titer. We have used this model to study the therapy of chronic viremia as well as chemoprophylaxis after viral exposure, both with single agents and with combination therapy. We showed that prompt ini...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150118
更新日期:1989-01-01 00:00:00