Nondetection of infectious hepatitis B virus in a human hepatoma cell line producing hepatitis B surface antigen.

Abstract:

:The PLC/PRF/5 human hepatoma cell line producing hepatitis B surface antigen (HBsAg) was studied to determine whether infectious hepatitis B virus (HBV) was also being produced. 2 chimpanzees with no previous exposure to HBV and no serologic markers of past or active HBV infection were inoculated intravenously with 50 ml of either tissue culture supernatant fluid (357 ng/ml HBsAg) or a suspension of cells disrupted by repeated freeze-thaw cycles (57 ng/ml HBsAg). No evidence of HBV infection was detected in either chimpanzee during 6 months of evaluation. This study suggests that the expression of a portion of the HBV genome, when a portion or all of that genome has been incorporated into a host cell, can result in the production of HBsAg without infectious HBV. If it becomes possible to produce a similar expression of this portion of the genome by itself in nonmalignant cells, HBsAg without HBV may be produced in vitro for use in hepatitis B vaccines.

journal_name

Intervirology

journal_title

Intervirology

authors

Tabor E,Copeland JA,Mann GF,Howard CR,Skelly J,Snoy P,Zuckerman AJ,Gerety RJ

doi

10.1159/000149217

subject

Has Abstract

pub_date

1981-01-01 00:00:00

pages

82-6

issue

2

eissn

0300-5526

issn

1423-0100

journal_volume

15

pub_type

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