Abstract:
:A whitefly-transmitted virus from Venezuela, potato yellow mosaic virus, has been propagated, isolated and characterized as a member of the Geminivirus group. The virus was transmitted to several species of Nicotiana and to Petunia hybrida by mechanical inoculation, and to potato and tomato plants by grafting. Purified virus possessed typical geminate particle morphology and encapsidated both genomic and subgenomic species consisting of single-stranded DNA. The genome of the virus was cloned into both bacteriophage and plasmid vectors following restriction of supercoiled double-stranded DNA species isolated from infected plant extracts. Two distinct classes of cloned DNA were generated, both about 2,500-2,600 base pairs in length and designated A and B, which when inoculated in combination to Nicotiana benthamiana were infectious after excision from the recombinant clones. The subgenomic DNA was shown to be related to DNA B.
journal_name
Intervirologyjournal_title
Intervirologyauthors
Roberts EJ,Buck KW,Coutts RHdoi
10.1159/000150042subject
Has Abstractpub_date
1988-01-01 00:00:00pages
162-9issue
3eissn
0300-5526issn
1423-0100journal_volume
29pub_type
杂志文章相关文献
INTERVIROLOGY文献大全abstract:OBJECTIVE:To screen and identify cellular proteins binding to the core region of hepatitis C virus (HCV) RNA genome. METHODS:The plasmid pHCV core was constructed to generate in vitro transcripts of the core region of HCV RNA genome. Ultraviolet (UV) cross-linking experiment and competition analysis were performed to ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000105443
更新日期:2007-01-01 00:00:00
abstract::Cytomegalovirus infection of Brown Norway rats was studied after intraperitoneal or subcutaneous inoculation of virus. No clinical illness was apparent during the 1st month postinfection (p.i.). Low titers of virus were detected in many organs at day 4 p.i. for the intraperitoneally inoculated animals and at day 11 p....
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149612
更新日期:1985-01-01 00:00:00
abstract::The Holland strain of anatid herpesvirus (AHV) was 2-to 10-fold more efficiently plaqued under liquid medium or semisolid medium containing methylcellulose than in media containing agar, agarose, or Nobel agar. Virus adsorption was complete in 45 min, with the maximum virus titers obtained under liquid medium at 48 h ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149193
更新日期:1980-01-01 00:00:00
abstract::Early hepatocellular carcinoma (HCC) as defined by the Liver Cancer Study Group of Japan would correspond to high-grade dysplastic nodules with small foci of HCC in the majority of cases, using the classification system proposed by the International Working Party. A large number of early HCCs were revealed to be hypo-...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000078473
更新日期:2004-01-01 00:00:00
abstract::Solubilized envelope antigens (glycoproteins) of Sendai virus were purified in a DEAE-Bio-Gel A column. Monospecific antisera were prepared against the antigens, designated HN and F glycoproteins. These glycoproteins are antigenically distinct. Immunodiffusion analyses of the envelope antigens of three representative ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149462
更新日期:1975-01-01 00:00:00
abstract:OBJECTIVE:Syncytia formation is the hallmark of the cytopathic effect caused by human respiratory syncytial virus (HRSV), which is the most important viral respiratory pathogen in children. This article reports methodological improvements in primary HRSV isolation and the importance of syncytia formation and mRNA level...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000480014
更新日期:2017-01-01 00:00:00
abstract:OBJECTIVE:To investigate the effect of HIV Tat protein on Bcl-2 expression in human monocytes, and observe apoptosis of Tat-stimulated monocytes induced by TNF-alpha-related apoptosis-induced ligand (TRAIL). METHODS:Western blot was used to detect Bcl-2 expression in monocytes stimulated by HIV Tat protein, and Annexi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000100565
更新日期:2007-01-01 00:00:00
abstract::Interferon induction in BSC-1 cells by measles virus isolates previously found to be interferon inducing (IF+) or interferon noninducing (IF-) was studied in different cell types. Lack of induction by the IF- viruses was not due to an inhibitory factor since BSC-1 cells coinfected with IF+ and IF- stocks produced inte...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149274
更新日期:1981-01-01 00:00:00
abstract:BACKGROUND:Arboviruses co-circulating within a population that are transmitted by the same vector have the potential to cause coinfections. Coinfections with dengue virus (DENV), Zika virus (ZIKV), and chikungunya virus (CHIKV) have been occurring in Brazil, but it is not well-understood how human responses vary during...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000510223
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVE:Because the determination of the stage of fibrosis depends on rather subjective judgment, more objective parameters are needed. In this study, we followed the long-term outcome, with monitoring of platelet counts, in patients with chronic hepatitis C or liver cirrhosis (LC) who had undergone interferon (IFN) ...
journal_title:Intervirology
pub_type: 临床试验,杂志文章
doi:10.1159/000073209
更新日期:2003-01-01 00:00:00
abstract::SDS-polyacrylamide slab gel analysis of selected primate and nonprimate adenoviruses has shown these viruses to differ significantly at the polypeptide molecular level. It follows that data derived from study of a single adenovirus serotype are unique to that serotype and are not representative of the adenovirus group...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149001
更新日期:1978-01-01 00:00:00
abstract::Hepatitis E virus (HEV), the main etiologic agent of enterically transmitted acute hepatitis in developing countries, is now recognized as an emerging agent of autochthonous disease and chronic hepatitis E in immunocompromised patients in Europe where HEV infection is probably zoonotically acquired. We describe the fi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000335664
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the exposure of piglets to enteroviruses-G (EV-G) through the presence of antibodies in their serum. METHODS:Serum samples were obtained from the vena cava of 10 piglets at 9 weeks of age and again 39 days later (day 39). They were tested using an immunoassay based on...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000449058
更新日期:2016-01-01 00:00:00
abstract::The effect of caffeine on cytomegalovirus (CMV) replication in human embryo lung (HEL) and human embryo kidney (HEK) cells was studied. In HEL cultures, nonirradiated CMV yielded up to 6.7 times more plaques in caffeine-treated cultures than in control cultures. Virus growth curves exhibited different profiles, depend...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000148987
更新日期:1978-01-01 00:00:00
abstract::Rotavirus is the most common cause of severe diarrhoea worldwide, affecting over 125 million young children every year in developing countries. The present study is a part of ongoing childhood diarrhoeal surveillance to determine the strain diversity of rotaviruses prevalent in Andaman and Nicobar Islands. From Octobe...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000342219
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:Characterization of the phylogeny and diversity of human respiratory syncytial virus (HRSV) genotype ON1 that occurred during its early evolution (within the first 3.5 years since the detection of the first ON1 strains). ON1 strains have a 72-nucleotide-long in-frame duplication within the second hypervariabl...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000382018
更新日期:2015-01-01 00:00:00
abstract::The effects of cyclosporin A (CsA) upon the pathogenesis of primary cytomegalovirus (CMV) infection were investigated. Hartley and strain 2 guinea pigs were inoculated subcutaneously on day 0 with 10(4) TCD50 of virulent, salivary-gland-passaged guinea pig CMV and received oral CsA (20 mg/kg/day) for 14 days. CMV-infe...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149635
更新日期:1985-01-01 00:00:00
abstract::Monoclonal antibodies were used to study measles virus (Hallé strain) infections. The distribution of virus antigens in acutely and persistently infected cells was examined by immunofluorescence, and the synthesis of the virus polypeptides in acutely infected cells was measured after immunoprecipitation and analysis i...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149509
更新日期:1984-01-01 00:00:00
abstract::To evaluate the diagnostic value of nucleic-acid-sequence-based amplification (NASBA) for the detection of cytomegalovirus (CMV) infection in transplant recipients, we compared immediate early 1 (IE1) and late pp67 mRNA detection by NASBA with the antigenemia assay, PCR and viral culture in 72 renal transplant (RTx) r...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000053974
更新日期:1999-01-01 00:00:00
abstract::The aim of the study was to determine the prevalence of drug resistance of HIV-1 in pediatric patients from Istanbul, Turkey. Genotypic drug resistance testing revealed transmission of drug resistance from mother to child in 20%. Due to rising numbers of children with HIV-1, baseline resistance testing is recommended ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000362335
更新日期:2014-01-01 00:00:00
abstract::Two closely related viruses were isolated from the blood of bovines near Darwin, Northern Territory, Australia. When studies of virus morphology indicated that these were rhabdoviruses, serologic studies were done. These isolates are closely related or identical and are related to, but distinct from, the rabies-relate...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150274
更新日期:1992-01-01 00:00:00
abstract::Human cytomegalovirus (HCMV), like all herpesviruses, persists in the host after primary infection, with reactivation often occurring as a result of immunosuppression. The lack of a model system has made analysis of HCMV latency and reactivation difficult. However, the ability to analyse which specific cell types in v...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000150501
更新日期:1996-01-01 00:00:00
abstract::The species concept seems to be inescapably based on the occurrence, in a population, of regular genetic interchange which leads to the formation of common gene pools and on the presence of barriers to interchange which distinguish one gene pool from another. Present knowledge indicates that some types of viruses (e.g...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149624
更新日期:1985-01-01 00:00:00
abstract::Membrane particles possessing receptor activity for influenza virions have been reconstituted following solubilization of human erythrocyte membranes with octylglucoside (OG) and fractionation on a DEAE-cellulose column. Fractions that eluted with 1.5 M NaCl yielded, after removal of OG, reconstituted membrane particl...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149334
更新日期:1983-01-01 00:00:00
abstract:AIMS:The aim of the present study is the preliminary characterization of an El Tor vibriophage M4 (ATCC 51352-B4). METHODS:We studied the growth characteristics and sustainability of this phage under various stresses like temperature, pH, and UV. The phage morphology and phage genome were also examined using electron ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000485835
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Infection with tick-borne encephalitis virus (TBEV) causes pathological changes in the central nervous system. However, the possible redox alterations in the infected cells that can contribute to the virus pathogenicity remain unknown. OBJECTIVE:In the current study we explored the ability of TBEV nonstruct...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000452160
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVE:We studied the ability of human cytomegalovirus (HCMV) to infect peripheral blood mononuclear cells (PBMC) pretreated with or without Th2-cytokine interleukin-4 (IL-4) in vitro. METHODS:Adherent cells and nonadherent cells were obtained from PBMC. We inoculated these cells with HCMV at concentrations ranging...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000050057
更新日期:2001-01-01 00:00:00
abstract::By a combination of PCR and direct-cycle sequencing using consensus primers, we analyzed approximately 400-bp fragments within the NS3 genes of twenty-one dengue virus type 3 strains isolated from five neighboring Southeast Asian countries at different time intervals from 1956 to 1992. The majority of base disparities...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150386
更新日期:1994-01-01 00:00:00
abstract::By 48 h postinfection, 40--80% of SV40-infected CV-1 cells have undergone irreversible injury as indicated by trypan blue staining. Nevertheless, at this time the lysosomes of these cells appear as discrete structures after vital staining with either acridine orange or neutral red. Lysosomes, vitally stained with neut...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149068
更新日期:1979-01-01 00:00:00
abstract::The leukocyte migration inhibition (LMI) technique was used to measure the T cell-mediated immune response of Epstein-Barr-virus (EBV)-seropositive human donors to antigens associated with B cell lines of simian origin, transformed by simian EBV-like viruses, Herpesvirus papio (HVP), H. pan, H. gorilla and H. pongo. E...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149691
更新日期:1986-01-01 00:00:00