A study of measles virus antigens in acutely and persistently infected cells using monoclonal antibodies: differences in the accumulation of certain viral proteins.

Abstract:

:Monoclonal antibodies were used to study measles virus (Hallé strain) infections. The distribution of virus antigens in acutely and persistently infected cells was examined by immunofluorescence, and the synthesis of the virus polypeptides in acutely infected cells was measured after immunoprecipitation and analysis in SDS-polyacrylamide gels. The virus nucleoprotein (NP) was detected 12 h after infection and was the major virus polypeptide synthesized throughout the virus cycle. The NP, matrix, and polymerase (L) polypeptides were first detected associated with the perinuclear membrane, but were later distributed throughout the cytoplasm. The hemagglutinin accumulated in the cell membrane in well-defined 'clusters'. In comparison with the acutely infected system, persistently infected cells presented important differences. There were only low levels of L polypeptides, and up to 50% of the cells contained intranuclear inclusions which stained specifically with NP monoclonal antibody. The relevance of these observations is discussed.

journal_name

Intervirology

journal_title

Intervirology

authors

Giraudon P,Gerald C,Wild TF

doi

10.1159/000149509

subject

Has Abstract

pub_date

1984-01-01 00:00:00

pages

110-20

issue

2

eissn

0300-5526

issn

1423-0100

journal_volume

21

pub_type

杂志文章
  • Barley yellow mosaic virus (BaYMV) and BaYMV-M: two different viruses.

    abstract::Serological and physical properties of isolated particles as well as reactions of barley cultivars after virus inoculation showed that the yellow disease of barley is caused by different viruses. Results summarized in this paper suggest that the European isolates BaYMV-So and BaYMV-NM are closely related to, or identi...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000150132

    authors: Huth W,Adams MJ

    更新日期:1990-01-01 00:00:00

  • In vitro interaction of scrapie agent and mouse peritoneal macrophages.

    abstract::Scrapie brain homogenate was mixed with mouse peritoneal macrophages in vitro. After 2 h of incubation at 37 degrees, a portion of the scrapie infectivity was associated with macrophages. In contrast, very little infectivity was associated with kidney cells that had been exposed to scrapie brain homogenate. After incu...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149241

    authors: Carp RI,Callahan SM

    更新日期:1981-01-01 00:00:00

  • Variability of the region of the herpes simplex virus type 1 genome yielding defective DNA: SmaI fragment polymorphism.

    abstract::A SmaI map of class I defective DNA of herpes simplex virus type 1 (HSV1) was constructed using a set of deletion hybrid phages. Four SmaI fragments on the defective DNA had a variability in length common among 15 HSV1 isolates: the 1.45 kilobase (kb) fragment located within the BamHI-Z (map coordinates 0.936-0.949) f...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149596

    authors: Umene K

    更新日期:1985-01-01 00:00:00

  • Nucleotide sequence of rice dwarf phytoreovirus genome segment 2: completion of sequence analyses of rice dwarf virus.

    abstract::The complete nucleotide sequence of rice dwarf phytoreovirus genome segment 2 (S2) was determined to be 3,512 nucleotides long with one open reading frame initiating at nucleotide 15 and terminating at nucleotide 3363. The encoded polypeptide was predicted to have 1,116 residues with a relative molecular weight of 123...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000150348

    authors: Uyeda I,Suda N,Yamada N,Kudo H,Murao K,Suga H,Kimura I,Shikata E,Kitagawa Y,Kusano T

    更新日期:1994-01-01 00:00:00

  • The genome organization of pea stem necrosis virus and its assignment to the genus Carmovirus.

    abstract::The complete sequence of the single-stranded, positive-sense RNA genome of pea stem necrosis virus (PSNV) has been determined. The 4,048-nucleotide genome contains five open reading frames (ORFs). The 5'-proximal ORF encodes a 25-kD protein (p25). If the amber termination codon is read through, the ORF produces a read...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000065870

    authors: Suzuki S,Hase S,Takahashi H,Ikegami M

    更新日期:2002-01-01 00:00:00

  • Physicochemical characterization of a small RNA virus associated with baculovirus infection in Trichoplusia ni.

    abstract::An RNA virus was isolated from Trichoplusia ni larvae infected with inoculum of the baculovirus of Autographa californica. Physicochemical characterization has revealed a 35-nm virus with a density in CsCl of 1.3 g/cm3 and a sedimentation coefficient of 200S. The virus consisted of 10--15% single-stranded RNA with an ...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149040

    authors: Morris TJ,Hess RT,Pinnock DE

    更新日期:1979-01-01 00:00:00

  • Molecular evolution of hepatitis viruses.

    abstract::Methods and models for analytical and quantitative researches on the evolution of hepatitis viruses using nucleotide sequences and amino acids of hepatitis viruses are described. Methods for the calculation of evolutionary rates and the construction of molecular evolutionary trees, especially by estimating the number ...

    journal_title:Intervirology

    pub_type: 杂志文章,评审

    doi:10.1159/000024975

    authors: Mizokami M,Orito E

    更新日期:1999-01-01 00:00:00

  • Evolutionary relationship of three southern African maize streak virus isolates.

    abstract::The aligned restriction endonuclease maps of three sequenced maize streak virus isolates, three restriction-mapped southern African maize streak virus isolates, and two other sequenced geminiviruses were used as a means of calculating the sequence divergence between these viruses. The degree of divergence was used to ...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000150080

    authors: Kirby R,Clarke BA,Rybicki EP

    更新日期:1989-01-01 00:00:00

  • Whole-genome sequence analysis of human papillomavirus type 18 from infected Thai women.

    abstract:OBJECTIVE:The aim of this study was to attain molecular knowledge of human papillomavirus type 18 (HPV18) by sequencing the whole genome of HPV18 isolated from Thai women at various clinical stages of disease progression. METHOD:Our group analyzed 9 samples of whole-genome HPV18 in infected women ranging from normal t...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000274977

    authors: Lurchachaiwong W,Junyangdikul P,Termrungruanglert W,Payungporn S,Sampatanukul P,Tresukosol D,Niruthisard S,Trivijitsilp P,Karalak A,Swangvaree S,Poovorawan Y

    更新日期:2010-01-01 00:00:00

  • Characterization of human Influenza Viruses in Lebanon during 2010-2011 and 2011-2012 post-pandemic seasons.

    abstract:OBJECTIVE:To genetically characterize human influenza viruses and their susceptibilities to antivirals during two post-pandemic seasons in Lebanon. METHODS:Influenza virus was isolated from nasopharyngeal swabs that were obtained from patients with influenza-like illness during 2010-2012 and further analyzed both phen...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000365758

    authors: Zaraket H,Dapat C,Ghanem S,Ali Z,Lteif M,Kondo H,Dapat IC,Saito K,Kayali G,Suzuki H,Dbaibo G,Saito R

    更新日期:2014-01-01 00:00:00

  • Comparison of proteins and nucleic acids of Echinochloa ragged stunt and rice ragged stunt viruses.

    abstract::Echinochloa ragged stunt virus (ERSV) and rice ragged stunt virus (RRSV; Reoviridae) were purified from their respective host plants and disrupted in SDS. Evidence for the double-strandedness of ERSV RNA was obtained. Electrophoresis in 10% polyacrylamide gels resolved the RNAs of each virus into 10 segments, ranging ...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000150103

    authors: Chen CC,Hsu YH,Chen MJ,Chiu RJ

    更新日期:1989-01-01 00:00:00

  • Mutational analysis of HBsAg assembly.

    abstract::The 20-nm noninfectious empty envelope particles carrying the hepatitis B surface antigen are secreted in large excess from hepatocytes during a hepatitis B infection. Similar particles produced in cell lines or yeast are an efficient vaccine against hepatitis B virus. We have analyzed the assembly of 20-nm particles ...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000150410

    authors: Prange R,Mangold CM,Hilfrich R,Streeck RE

    更新日期:1995-01-01 00:00:00

  • Syncytia Induction by Clinical Isolates of Human Respiratory Syncytial Virus A.

    abstract:OBJECTIVE:Syncytia formation is the hallmark of the cytopathic effect caused by human respiratory syncytial virus (HRSV), which is the most important viral respiratory pathogen in children. This article reports methodological improvements in primary HRSV isolation and the importance of syncytia formation and mRNA level...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000480014

    authors: Gagliardi TB,Criado MF,Proença-Módena JL,Saranzo AM,Iwamoto MA,de Paula FE,Cardoso RS,Delcaro LS,Silva ML,Câmara AA,Arruda E

    更新日期:2017-01-01 00:00:00

  • Construction of rat cell lines that contain potential morphologically transforming regions of the herpes simplex virus type 2 genome.

    abstract::Hybrid recombinant plasmids were constructed; they were composed of the herpes simplex virus type 2 (HSV2) thymidine kinase (tk) gene and DNA sequences of HSV2 that have been reported to induce morphological and/or oncogenic transformation of rodent cells in culture. Several plasmids were made in two versions, with or...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149644

    authors: van den Berg FM,van Amstel PJ,Walboomers JM

    更新日期:1985-01-01 00:00:00

  • The XTrack system: application and advantage.

    abstract::XTrack, a rapid, cost-effective diagnostic HIV tropism system, yields predictive results and dissects virus mixtures. It is based on the duplexing of patient samples with selective DNA probes, combined with sequence-based analysis and replicative phenotyping for ambiguous samples. ...

    journal_title:Intervirology

    pub_type: 杂志文章,评审

    doi:10.1159/000332003

    authors: Klimkait T

    更新日期:2012-01-01 00:00:00

  • Numerical diagnoses of superinfection in chronic hepatitis B viral dynamics.

    abstract:OBJECTIVES:Fluctuation profile has been observed in chronic hepatitis B patients who are untreated or interrupt therapy. A mathematical model and its parameters could be used to diagnose the assumption of superinfection of hepatocytes and to understand the causes for the spontaneous fluctuation pattern of HBV DNA loads...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000321454

    authors: Wang K,Tan W,Tang Y,Deng G

    更新日期:2011-01-01 00:00:00

  • Cyclic appearance of defective interfering particles of herpes simplex virus and the concomitant accumulation of early polypeptide VP175.

    abstract::Serial passage of undiluted herpes simplex virus types 1 and 2 resulted in cyclic production of infectious and defective virions. Defective virus production was characterized by the appearance of a new species of viral DNA with a higher bouyant density in CsCl than standard viral DNA. Measurement of the infectivity ti...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149894

    authors: Murray BK,Biswal N,Bookout JB,Lanford RE,Courtney RJ,Melnick JL

    更新日期:1975-01-01 00:00:00

  • Expression of E1E2 on hepatitis C RNA-containing particles released from primary cultured human hepatocytes derived from infected cirrhotic livers.

    abstract:OBJECTIVE:To determine whether liver-derived hepatitis C RNA-containing particles express the E1E2 discontinuous antigenic determinant defined by unique monoclonal antibody (mAb) D32.10 which recognizes three highly conserved segments in E1 (aa297-306) and E2 (aa480-494 and aa613-621) envelope glycoproteins. METHODS:H...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000318886

    authors: Ndongo N,Selliah S,Berthillon P,Raymond VA,Trépo C,Bilodeau M,Petit MA

    更新日期:2011-01-01 00:00:00

  • Comparative polypeptide patterns of representative human and nonhuman adenoviruses.

    abstract::SDS-polyacrylamide slab gel analysis of selected primate and nonprimate adenoviruses has shown these viruses to differ significantly at the polypeptide molecular level. It follows that data derived from study of a single adenovirus serotype are unique to that serotype and are not representative of the adenovirus group...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149001

    authors: Marusyk RG,Cummings GT

    更新日期:1978-01-01 00:00:00

  • Respiratory syncytial virus enhances the expression of CD11b molecules and the generation of superoxide anion by human eosinophils primed with platelet-activating factor.

    abstract:BACKGROUND:Human respiratory syncytial virus (RSV) infection in infancy and early childhood causes acute bronchiolitis and exacerbates bronchial asthma. Eosinophil infiltration may contribute to airway obstruction in RSV infection. OBJECTIVE:We hypothesized that RSV affects eosinophil function. METHODS:Eosinophil act...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000050086

    authors: Tachibana A,Kimura H,Kato M,Nako Y,Kozawa K,Morikawa A

    更新日期:2002-01-01 00:00:00

  • Some properties of RNA polymerases of different influenza viruses.

    abstract::The transcriptases of several influenza viruses were tested for pH and temperature dependence and for thermal stability. Marked differences in pH dependence and thermostability were determined. ...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149012

    authors: Broucek J,Závadová H,Vonka V

    更新日期:1979-01-01 00:00:00

  • Scrapie-associated tubulofilamentous particles in scrapie hamsters.

    abstract::Examination of thin sections from the cerebral cortex of scrapie-infected hamster brains revealed characteristic circular 26-30 nm diameter tubulofilamentous particles, identical to those previously described in both experimentally induced scrapie in mice, hamsters and natural scrapie of sheep, bovine spongiform encep...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000150269

    authors: Narang HK

    更新日期:1992-01-01 00:00:00

  • Immunoelectrophoretic identification and purification of herpes simplex virus antigens released from infected cells in tissue culture.

    abstract::Proteins released from herpes simplex virus type 1 (HSV-1)-and type 2 (HSV-2)-infected HEp-2 cells have been characterized by the crossed immunoelectrophoretic technique. Both HSV type-common and type-specific antigens were found in the tissue culture medium 24 h after infection. Antigen Ag-6, an HSV-1-specific antige...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149020

    authors: Norrild B,Vestergaard BF

    更新日期:1979-01-01 00:00:00

  • Havachoobe (Onosma dichroanthum Boiss) Root Extract Decreases the Hepatitis B Virus Surface Antigen Secretion in the PLC/PRF/5 Cell Line.

    abstract:BACKGROUND:Many efforts are currently focused on functional treatment of the hepatitis B virus (HBV). This can be done by suppressing the secretion of HBV surface antigen (HBsAg). Scientific communities are very interested in natural products in that respect. OBJECTIVE:Use of root extract of Havachoobe (Onosma dichroa...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000512140

    authors: Mohebbi A,Azadi F,Hashemi MM,Askari FS,Razzaghi N

    更新日期:2020-12-15 00:00:00

  • Use of Multiplex Polymerase Chain Reaction for Detection of High-Risk Human Papillomavirus Genotypes in Women Attending Routine Cervical Cancer Screening in Harare.

    abstract:BACKGROUND/AIMS:In Zimbabwe, cervical cancer is screened through cytology and visual inspection with acetic acid and cervicography (VIAC). The effectiveness of these methods can be increased if complemented by a human papillomavirus DNA detection tool since most cervical cancer cases are caused by persistent infection ...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000502206

    authors: Marembo T,Dube Mandishora R,Borok M

    更新日期:2019-01-01 00:00:00

  • Isolation and morphology of the internal component of human coronavirus, strain 229E.

    abstract::Biochemical studies on human coronavirus, strain 229E, indicate that the RNA is present in the virion in association with protein as a ribonucleoprotein (RNP) complex. Morphological studies have revealed that this nucleocapsid is probably a continuous helical RNP. ...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149474

    authors: Kennedy DA,Johnson-Lussenburg CM

    更新日期:1975-01-01 00:00:00

  • Characterization of potato yellow mosaic virus as a geminivirus with a bipartite genome.

    abstract::A whitefly-transmitted virus from Venezuela, potato yellow mosaic virus, has been propagated, isolated and characterized as a member of the Geminivirus group. The virus was transmitted to several species of Nicotiana and to Petunia hybrida by mechanical inoculation, and to potato and tomato plants by grafting. Purifie...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000150042

    authors: Roberts EJ,Buck KW,Coutts RH

    更新日期:1988-01-01 00:00:00

  • Special aspects of the treatment of HIV-2-infected patients.

    abstract::HIV-2 is responsible for a limited epidemic in West Africa. Around 20% of all infected patients will progress to AIDS, and will need antiretroviral therapy. Unfortunately, antiretrovirals were developed to suppress HIV-1 replication; not all of them are active against HIV-2, e.g. all nonnucleoside reverse transcriptas...

    journal_title:Intervirology

    pub_type: 杂志文章,评审

    doi:10.1159/000332025

    authors: Camacho RJ

    更新日期:2012-01-01 00:00:00

  • Membrane-Associated Kaposi Sarcoma-Associated Herpesvirus Glycoprotein B Promotes Cell Adhesion and Inhibits Migration of Cells via Upregulating IL-1β and TNF-α.

    abstract:OBJECTIVES:Kaposi sarcoma-associated herpesvirus (KSHV) glycoprotein B (gB) is expressed on the viral envelope as well as on the cytoplasmic membrane of infected cells. In the current study, we aimed to decipher the impact of membrane-associated gB on adhesion and migration of cells via modulating the expression of cyt...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000487596

    authors: Hussein HAM,Abdel-Raouf UM,Akula SM

    更新日期:2017-01-01 00:00:00

  • Cytomegalovirus glycoprotein B genotypes in Mexican children and women.

    abstract:OBJECTIVE:Cytomegalovirus (CMV) is widely distributed and constitutes the main cause of congenital infections worldwide. CMV transmission during pregnancy represents one of the major impacts of this virus on public health. This study aimed at assessing glycoprotein B (gB) CMV genotypes in Mexican children and pregnant ...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000373922

    authors: Gonzalez-Sanchez HM,Alvarado-Hernandez DL,Guerra-Palomares S,Garcia-Sepulveda CA,Noyola DE

    更新日期:2015-01-01 00:00:00