Abstract:
BACKGROUND:Human respiratory syncytial virus (RSV) infection in infancy and early childhood causes acute bronchiolitis and exacerbates bronchial asthma. Eosinophil infiltration may contribute to airway obstruction in RSV infection. OBJECTIVE:We hypothesized that RSV affects eosinophil function. METHODS:Eosinophil activation was evaluated by chemiluminescent detection of superoxide anion (O(2)(-)) generation. Expression of CD11b on eosinophils was determined by flow cytometry. RESULTS:Although RSV did not induce O(2)(-) generation by resting eosinophils, RSV enhanced O(2)(-) generation of eosinophils primed with platelet-activating factor (PAF). Enhancement was significantly inhibited by either continuous agitation to prevent eosinophil adhesion to test tube surfaces or by pretreating cells with anti-CD18 antibody, suggesting that the stimulatory effects of RSV on eosinophils depend on cell adhesion via beta(2)-integrins. In fact, RSV enhanced PAF-induced CD11b expression by eosinophils. CONCLUSIONS These findings suggest that RSV enhances eosinophil CD11b expression and O(2)(-) generation induced by PAF. Thus, RSV infection may exacerbate airway inflammation by enhancing mediator release from eosinophils.
journal_name
Intervirologyjournal_title
Intervirologyauthors
Tachibana A,Kimura H,Kato M,Nako Y,Kozawa K,Morikawa Adoi
10.1159/000050086keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
43-51issue
1eissn
0300-5526issn
1423-0100pii
50086journal_volume
45pub_type
杂志文章相关文献
INTERVIROLOGY文献大全abstract::Rotavirus is the most common cause of severe diarrhoea worldwide, affecting over 125 million young children every year in developing countries. The present study is a part of ongoing childhood diarrhoeal surveillance to determine the strain diversity of rotaviruses prevalent in Andaman and Nicobar Islands. From Octobe...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000342219
更新日期:2013-01-01 00:00:00
abstract::Monoclonal antibodies were used to study measles virus (Hallé strain) infections. The distribution of virus antigens in acutely and persistently infected cells was examined by immunofluorescence, and the synthesis of the virus polypeptides in acutely infected cells was measured after immunoprecipitation and analysis i...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149509
更新日期:1984-01-01 00:00:00
abstract:OBJECTIVE:Transmission of HIV-1 and diagnosis of infection in hospitals and public health settings remains a worldwide concern. HIV-1 detection is sometimes not possible using current commercial assays, probably due to mismatches between the selected primers and probes. METHODS:By screening primers and probes, we deve...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000119119
更新日期:2008-01-01 00:00:00
abstract::There are two major requirements for potyvirus taxonomy; group-specific criteria and criteria which discriminate between distinct viruses and strains. This review discusses the relative merits in potyvirus taxonomy of molecular parameters, such as gene sequence data, nucleic acid hybridization, coat protein sequence d...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000150211
更新日期:1991-01-01 00:00:00
abstract::Transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhea virus (PEDV) are similar coronaviruses, causing diseases characterized by vomiting, diarrhea, and death from severe dehydration in piglets. Thus, they have caused huge losses to the swine-breeding industry worldwide. Nowadays, they are easily tra...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000492424
更新日期:2018-01-01 00:00:00
abstract::The envelope glycoprotein of the mouse mammary tumor virus (MMTV gp52), a useful plasma marker for mammary tumors in mice, was measured in extracellular medium by radioimmunoassay to quantitatively compare gp52 levels released by a C3H MMTV producer and its subcloned B9 nonproducer cell line. Experiments analyzed leve...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150081
更新日期:1989-01-01 00:00:00
abstract:OBJECTIVE:To investigate the relationship between enteroviral infection and myocardial tissue apoptosis during the development of viral myocarditis in a murine model. METHODS:C3H/HeJ mice were inoculated with two strains of coxsackievirus B3, specifically CVB3 (cardiovirulent Nancy strain) and CVB3/0 (noncardiovirulen...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000071453
更新日期:2003-01-01 00:00:00
abstract::Dengue and chikungunya fevers are transmitted by the common mosquito vector Aedes and malaria by Anopheles. Concurrent infections are reported due to co-circulation of these pathogens, especially in endemic regions. We report a rare case of triple infection with 3 arthropod-borne pathogens (Plasmodium vivax and the de...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000479430
更新日期:2017-01-01 00:00:00
abstract::In this article, a statistical score method for comparison of partial genomic regions in their representatives of full-length genome of hepatitis E virus (HEV) for genotyping was developed. The critical values of the statistics for hypothesis testing were generated through Monte Carlo simulations. It was found that fr...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000106805
更新日期:2007-01-01 00:00:00
abstract::Interferon induction in BSC-1 cells by measles virus isolates previously found to be interferon inducing (IF+) or interferon noninducing (IF-) was studied in different cell types. Lack of induction by the IF- viruses was not due to an inhibitory factor since BSC-1 cells coinfected with IF+ and IF- stocks produced inte...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149274
更新日期:1981-01-01 00:00:00
abstract:OBJECTIVE:We studied the ability of human cytomegalovirus (HCMV) to infect peripheral blood mononuclear cells (PBMC) pretreated with or without Th2-cytokine interleukin-4 (IL-4) in vitro. METHODS:Adherent cells and nonadherent cells were obtained from PBMC. We inoculated these cells with HCMV at concentrations ranging...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000050057
更新日期:2001-01-01 00:00:00
abstract:OBJECTIVES:To explore the host transcription factor E2F-1 and/or any mechanisms that may support viral entry and its effect on the HSV-1 production in anti-CD3-activated Jurkat cells. METHODS:The expressions of ICP4, HVEM and E2F-1 were studied using reverse transcription-PCR and Western blot. HSV-1 production was det...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000437264
更新日期:2015-01-01 00:00:00
abstract::Three independently isolated herpes simplex virus type 1 recombinant viruses containing a deletion of approximately 14 kilobase pairs, representing greater than 95% of the internal repeat DNA sequences, were analyzed for their pathogenicity in mice. The recombinant viruses were found to be avirulent, exhibiting drasti...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150147
更新日期:1990-01-01 00:00:00
abstract::In Spodoptera frugiperda (SF IPLB-21) cells productively infected with S. frugiperda multicapsid nuclear polyhedrosis virus (SfMNPV), we observed the synthesis of infected-cell-specific polypeptides (ICSPs) using the protein synthesis inhibitor cycloheximide (CX) and the DAN synthesis inhibitor cytosine arabinoside (A...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150520
更新日期:1997-01-01 00:00:00
abstract::Marek's disease herpesvirus (MDHV) isolated from chickens free of naturally occurring avian leukosis virus (ALV) infection produced characteristic foci in both chicken embryo fibroblast (CEF) and chicken kidney cell (CKC) cultures. MDHV-A, which was extracted from the feather follicle epithelium of chickens naturally ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000148963
更新日期:1978-01-01 00:00:00
abstract::Hepatitis B virus (HBV) core antigen (HBcAg) detected by the peroxidase-antiperoxidase technique was present in the nucleus and cytoplasm of some infected hepatocytes but only in the cytoplasm of other hepatocytes. When cells expressing HBcAg were examined by in situ hybridization for the presence of HBV DNA, the intr...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149646
更新日期:1985-01-01 00:00:00
abstract::9-beta-D-Arabinofuranosyladenine (ara A) was shown to inhibit a specific step of tobacco mosaic virus (TMV) multiplication. The time course of the ara A-sensitive function occurred 'late' coinciding with the period of viral RNA and protein synthesis. When treatment began after viral RNA synthesis was established, 1.0 ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149694
更新日期:1986-01-01 00:00:00
abstract::We examined the effect of infection by other herpesviruses on the induction of Epstein-Barr virus (EBV) antigens. Raji cells were infected with a variety of viruses before superinfection with P3HR-1 virus 6 h later. Much earlier and greater synthesis of EBV antigens was observed in herpesvirus (herpes simplex virus ty...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150057
更新日期:1988-01-01 00:00:00
abstract:OBJECTIVES:Kaposi sarcoma-associated herpesvirus (KSHV) glycoprotein B (gB) is expressed on the viral envelope as well as on the cytoplasmic membrane of infected cells. In the current study, we aimed to decipher the impact of membrane-associated gB on adhesion and migration of cells via modulating the expression of cyt...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000487596
更新日期:2017-01-01 00:00:00
abstract:OBJECTIVE:To investigate the correlation of serum hepatitis B e antigen (HBeAg) levels with the presence of core promoter (CP) mutations, hepatitis B virus (HBV) viremia and the response to interferon (IFN) in patients with chronic hepatitis B. METHODS:Fourteen HBeAg-positive patients received alpha-2a IFN. Diluted se...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000072431
更新日期:2003-01-01 00:00:00
abstract::Infection with hepatitis B virus (HBV) continues to be a major problem in the human population. There remains a specific requirement for HBV vaccines capable of circumventing the non-responder/inadequate responder status of some vaccinees. Hepacare has been primarily developed to (1) improve anti-SHBs antibody titres ...
journal_title:Intervirology
pub_type: 临床试验,杂志文章
doi:10.1159/000050036
更新日期:2001-01-01 00:00:00
abstract::Hepatitis E virus (HEV), the main etiologic agent of enterically transmitted acute hepatitis in developing countries, is now recognized as an emerging agent of autochthonous disease and chronic hepatitis E in immunocompromised patients in Europe where HEV infection is probably zoonotically acquired. We describe the fi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000335664
更新日期:2012-01-01 00:00:00
abstract::Human skin fibroblasts have previously been reported to display an age-dependent resistance to infection with coxsackie B4 virus. We have shown that the virus will replicate and produce CPE in human skin fibroblasts regardless of the age of the donor of the cells. The passage history of the virus was found to influenc...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150029
更新日期:1988-01-01 00:00:00
abstract::SDS-polyacrylamide slab gel analysis of selected primate and nonprimate adenoviruses has shown these viruses to differ significantly at the polypeptide molecular level. It follows that data derived from study of a single adenovirus serotype are unique to that serotype and are not representative of the adenovirus group...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149001
更新日期:1978-01-01 00:00:00
abstract::Early hepatocellular carcinoma (HCC) as defined by the Liver Cancer Study Group of Japan would correspond to high-grade dysplastic nodules with small foci of HCC in the majority of cases, using the classification system proposed by the International Working Party. A large number of early HCCs were revealed to be hypo-...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000078473
更新日期:2004-01-01 00:00:00
abstract::Intrauterine viral infections show a wide range of fetal involvement with variable fetal outcome. The histopathologic changes of the placenta during viral infection show a wide spectrum. The most active processes are proliferative with a variety of cells involved. Vascular changes, including inflammatory and obliterat...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000024940
更新日期:1998-01-01 00:00:00
abstract::Cytomegalovirus infection of Brown Norway rats was studied after intraperitoneal or subcutaneous inoculation of virus. No clinical illness was apparent during the 1st month postinfection (p.i.). Low titers of virus were detected in many organs at day 4 p.i. for the intraperitoneally inoculated animals and at day 11 p....
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149612
更新日期:1985-01-01 00:00:00
abstract::The amino acid composition (AAC) of the coat proteins (CPs) of 126 plant viruses or strains were analyzed by stepwise discriminant analysis. The criteria chosen for discrimination were: the structure of virus particles (3 clusters); the mode of of transmission of the viruses (6 clusters); and the grouping of viruses a...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149675
更新日期:1986-01-01 00:00:00
abstract::Immunopathologic mechanisms leading to liver tissue injury in hepatitis caused by hepatitis A virus (HAV) were studied in an autologous in vitro model. Data show virus-specific killing by liver-infiltrating T lymphocytes in man and support the hypothesis that hepatocellular damage as well as efficient elimination of v...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150304
更新日期:1993-01-01 00:00:00
abstract:OBJECTIVE:Giant viruses of the Megavirales order have been neglected in the literature because they are removed from samples during viral purification for viral metagenomic studies. Isolation via amoeba coculture has low efficiency and is extremely time-consuming. Thus, our objective was to improve Megavirales detectio...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000354566
更新日期:2013-01-01 00:00:00