Abstract:
OBJECTIVE:We studied the ability of human cytomegalovirus (HCMV) to infect peripheral blood mononuclear cells (PBMC) pretreated with or without Th2-cytokine interleukin-4 (IL-4) in vitro. METHODS:Adherent cells and nonadherent cells were obtained from PBMC. We inoculated these cells with HCMV at concentrations ranging from 0 to 10 ng/ml of IL-4. Immediate-early antigen-1 (IE-1) and glycoprotein H (gH) mRNAs were detected using the reverse-transcription polymerase chain reaction. RESULTS:IE-1 and gH mRNAs could be detected in monocytes pretreated with IL-4. In contrast, no IE-1 mRNA was detected in monocytes pretreated without IL-4. We tested whether higher infectious titers could result in the infection of monocytes whether or not they were pretreated with IL-4. However, no IE-1 mRNA was detected in the monocytes not pretreated with IL-4. To elucidate how HCMV-infected monocytes affect lung tissue, human embryonic lung fibroblasts MRC-5 were cocultured with HCMV-infected monocytes. The cytopathic effects of HCMV were observed microscopically and was confirmed by direct immunoperoxidase staining with a human monoclonal antibody against the HCMV IE-1. CONCLUSION:Our data strongly suggest that the ability of HCMV to infect monocytes may correlate with the presence of IL-4.
journal_name
Intervirologyjournal_title
Intervirologyauthors
Kitajima H,Okubo Y,Honda J,Yonemitsu J,Yoshida N,Fumimori T,Oizumi Kdoi
10.1159/000050057keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
264-70issue
4eissn
0300-5526issn
1423-0100pii
50057journal_volume
44pub_type
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