Alteration in the coding potential and expression of H-ras in human cytomegalovirus-transformed cells.

Abstract:

:CMV-transformed cell lines demonstrated a greater level of H-ras RNA (7.5- to 9.5-fold) relative to the level for H-ras in parental cells. Nuclear run off assays showed that the RNA levels for the H-ras gene were regulated at the level of transcriptional initiation. The increased RNA levels for H-ras correlated with the level of p21rasVal-12 in transformed cells, while p21rasVal-12 was below the level of detection in nontransformed cells using Western blot analysis. In addition, an activating mutation was identified in both alleles of the first exon, codon 12 of H-ras resulting in a G:C to T:A transversion in all transformed cell lines examined in this study. These results suggest that the mutated H-ras may be one of the components by which an oncogenic phenotype is maintained in these CMV-transformed cells.

journal_name

Intervirology

journal_title

Intervirology

authors

Boldogh I,Huang ES,Rady P,Arany I,Tyring S,Albrecht T

doi

10.1159/000150396

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

321-9

issue

6

eissn

0300-5526

issn

1423-0100

journal_volume

37

pub_type

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