Type B fulminant hepatitis is closely associated with a highly mutated hepatitis B virus strain.

Abstract:

OBJECTIVE:Genome-wide sequences of hepatitis B virus strain associated with type B fulminant hepatitis have not been compared with those of acute self-limited hepatitis. We carried out full-length sequencing analysis of viral strains derived from patients with type B acute liver injury. METHODS:Nine acute self-limited hepatitis and 6 fulminant hepatitis patients were the subjects of this study. Full-length sequencing analysis of viral DNA was done by PCR-direct sequencing. RESULTS:Higher frequencies in fulminant hepatitis strains compared with acute hepatitis ones were observed in the T1762/A1764 (p < 0.05), A1896 (p = 0.09) and M1753 (M = C or A) (p = 0.09) mutations. Viruses related to fulminant hepatitis possessed the higher number of nucleotide substitutions than those related to acute hepatitis in the whole virus genome (p < 0.01) and various regions including preS/S gene (p < 0.05), precore/core gene (p < 0.01), polymerase gene (p < 0.05) and basic core promoter/core upstream regulatory sequence (p < 0.01). The high number of nucleotide substitutions in viruses related to fulminant hepatitis was predominantly non-synonymous in the preS/S and precore/core genes. CONCLUSION:Development of type B fulminant hepatitis may be associated with a highly mutated hepatitis B virus strain.

journal_name

Intervirology

journal_title

Intervirology

authors

Kanada A,Takehara T,Ohkawa K,Tatsumi T,Sakamori R,Yamaguchi S,Uemura A,Kohga K,Sasakawa A,Hikita H,Hijioka T,Katayama K,Deguchi M,Kagita M,Kanto T,Hiramatsu N,Hayashi N

doi

10.1159/000110651

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

394-401

issue

6

eissn

0300-5526

issn

1423-0100

pii

000110651

journal_volume

50

pub_type

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