Abstract:
BACKGROUND:Many efforts are currently focused on functional treatment of the hepatitis B virus (HBV). This can be done by suppressing the secretion of HBV surface antigen (HBsAg). Scientific communities are very interested in natural products in that respect. OBJECTIVE:Use of root extract of Havachoobe (Onosma dichroanthum BoissI), a Northern Iranian native medical herb, for assessment of its anti-HBsAg secretion activity. METHODS:Havachoobe had been bought at a nearby apothecary store. Plant root extract was obtained using a hydroalcoholic process. Cytotoxic activity of the extract was examined on PLC/PRF/5 cells using MTT assay. ELISA has been used to measure HBsAg in the treated cell line supernatants. In addition, real-time PCR analysis was performed to evaluate the expression of HBsAg before and after treatment of Onosma in vitro. RESULTS:The results showed very low root extract cytotoxicity at concentrations under 8 μg/mL. Tissue culture infectious dose 50 was obtained at 63.78 μg/mL. In a dose-dependent and time-dependent manner, a significantly reduced HBsAg secretion was observed at a concentration of 8 ppm at 12 h post-treatment. The real-time PCR result showed relative decreased HBsAg expression at all doses at 12 h post-treatment time. DISCUSSION:In this study, we first reported anti-HBsAg activity on an Iranian herbal medicine. Havachoobe root extract was shown to be able to inhibit HBsAg in a dose-dependent and time-dependent manner. We find the extract exerts its inhibitory effect of HBsAg by targeting transcription of HBsAg.
journal_name
Intervirologyjournal_title
Intervirologyauthors
Mohebbi A,Azadi F,Hashemi MM,Askari FS,Razzaghi Ndoi
10.1159/000512140subject
Has Abstractpub_date
2020-12-15 00:00:00pages
1-5eissn
0300-5526issn
1423-0100pii
000512140pub_type
杂志文章相关文献
INTERVIROLOGY文献大全abstract:OBJECTIVES:Data on drug-resistant mutations (DRMs) in HIV-1-infected therapy-naïve children are scarce. The aim of this study was to determine the HIV-1 subtype distribution and the prevalence of DRMs in therapy-naïve HIV-1-infected children who received routine care at the University Hospital Düsseldorf, Düsseldorf, G...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000477811
更新日期:2016-01-01 00:00:00
abstract::The ability to discriminate between herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) infections by serological means is of increasing importance for clinical virological diagnostics. HSV type-specific serology has a number of applications, e.g. to guide duration and dosage of antiviral therapy, to allow for string...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000150493
更新日期:1996-01-01 00:00:00
abstract::The species concept seems to be inescapably based on the occurrence, in a population, of regular genetic interchange which leads to the formation of common gene pools and on the presence of barriers to interchange which distinguish one gene pool from another. Present knowledge indicates that some types of viruses (e.g...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149624
更新日期:1985-01-01 00:00:00
abstract::The morphology, assembly, and accumulation sites of rhabdovirus particles in Peregrinus maidis planthoppers infected with a Hawaiian isolate of maize mosaic virus (MMV) were studied. These particles were usually bullet-shaped, but were sometimes bacilliform, and averaged 234 and 247 nm, respectively, in length and 60 ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149616
更新日期:1985-01-01 00:00:00
abstract:OBJECTIVE:To investigate the effect of HIV Tat protein on Bcl-2 expression in human monocytes, and observe apoptosis of Tat-stimulated monocytes induced by TNF-alpha-related apoptosis-induced ligand (TRAIL). METHODS:Western blot was used to detect Bcl-2 expression in monocytes stimulated by HIV Tat protein, and Annexi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000100565
更新日期:2007-01-01 00:00:00
abstract::Human skin fibroblasts were previously shown to be resistant to coxsackie B4 virus infection. We have cultured fibroblasts from skin and lung tissues of donors of various ages. By a novel method for direct assay of virus absorption and penetration, we have shown that skin fibroblasts from young fetuses are susceptible...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149360
更新日期:1983-01-01 00:00:00
abstract:OBJECTIVES:In the present study, we aimed to determine whether signal-to-cutoff (S/Co) ratios of reactive anti-HCV samples could be used as a basis for avoiding the need for supplemental testing in our study population. METHODS:We analyzed 901 anti-HCV-positive sera from 8 institutions in China. The Ortho VITROS anti-...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000441474
更新日期:2015-01-01 00:00:00
abstract::Studies of human papillomavirus (HPV) infection are hampered by the lack of an adequate culture system. The athymic mouse xenograft system permits propagation of HPV, but only a few HPV types have been grown in this manner. To produce an oncogenic type for studies of HPV pathogenesis, a condylomata acuminata lesion fr...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000025033
更新日期:2000-01-01 00:00:00
abstract::Analysis of translation products synthesized in vitro in the presence of a mixture of brome mosaic virus (BMV) RNAs 1, 2, 3, and 4 usually shows a predominance of coat protein, coded by RNA4. The proportion of products directed by RNAs 1 and 2 decreased at saturating concentrations of BMV RNA and in the presence of 7-...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149008
更新日期:1979-01-01 00:00:00
abstract:AIMS:The aim of the present study is the preliminary characterization of an El Tor vibriophage M4 (ATCC 51352-B4). METHODS:We studied the growth characteristics and sustainability of this phage under various stresses like temperature, pH, and UV. The phage morphology and phage genome were also examined using electron ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000485835
更新日期:2017-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the distribution of hepatitis C virus (HCV) genotype and the possible association between genotype and HCV-associated liver disease in Indonesia. METHODS:32 anti-HCV-positive asymptomatic carriers (AC), 55 chronic hepatitis (CH), 41 liver cirrhosis (LC), and 35 hepato...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000205515
更新日期:2008-01-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) latency was established in human embryo lung (HEL) cells by treatment with (E)-5-(2'-bromovinyl)-2'-deoxyuridine and human leukocyte interferon and subsequent temperature increase (from 37 degrees to 40.5 degrees). This in vitro system was used to study reactivation after temperatur...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149668
更新日期:1986-01-01 00:00:00
abstract:OBJECTIVE:Torque teno virus (TTV) is a ubiquitous virus that is commonly associated with blood transfusion. The main aims of this study were to evaluate the incidence of TTV in polytransfused children with thalassemia and to determine for the first time the prevalent TTV genotypes in Egypt. METHODS:TTV was detected in...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000480507
更新日期:2017-01-01 00:00:00
abstract:OBJECTIVE:Mycosis fungoides (MF) is the most frequent form of cutaneous T cell lymphoma (CTCL). Human T cell leukemia virus type 1 (HTLV-1) involvement in MF progression is a matter of debate. The goal of the investigation was to search for HTLV-1 markers in a group of MF patients from a nonendemic area to HTLV-1. MAT...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000086063
更新日期:2005-01-01 00:00:00
abstract::In this article, a statistical score method for comparison of partial genomic regions in their representatives of full-length genome of hepatitis E virus (HEV) for genotyping was developed. The critical values of the statistics for hypothesis testing were generated through Monte Carlo simulations. It was found that fr...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000106805
更新日期:2007-01-01 00:00:00
abstract:OBJECTIVES:Chikungunya virus (CHIKV) is an alphavirus belonging to the Togaviridae family. Alphaviruses cause a chronic non-cytopathic infection in mosquito cells, while they develop a highly cytopathic infection in cells originating from various vertebrates. In this study, we compared the cytopathic effect (CPE) induc...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000339985
更新日期:2013-01-01 00:00:00
abstract::This review summarizes current strategies for the treatment of human cytomegalovirus (CMV) infection/diseases in high-risk patients such as transplant recipients and AIDS patients. Since the major drugs ganciclovir (Cytovene), foscarnet (Foscavir) and cidofovir (Vistide) are frequently associated with severe side effe...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000150568
更新日期:1997-01-01 00:00:00
abstract::Rauscher murine leukemia virus (RLV) infection of adult mice results in splenomegaly that is proportional to the virus titer. We have used this model to study the therapy of chronic viremia as well as chemoprophylaxis after viral exposure, both with single agents and with combination therapy. We showed that prompt ini...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150118
更新日期:1989-01-01 00:00:00
abstract::The envelope glycoprotein of the mouse mammary tumor virus (MMTV gp52), a useful plasma marker for mammary tumors in mice, was measured in extracellular medium by radioimmunoassay to quantitatively compare gp52 levels released by a C3H MMTV producer and its subcloned B9 nonproducer cell line. Experiments analyzed leve...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150081
更新日期:1989-01-01 00:00:00
abstract::Cancer of the cervix is the most common malignant tumor among women in Africa and, in particular, Senegal. Studies of the prevalence of human papilloma virus (HPV) infection in Africa have mainly focused on carcinomas. Data on the presence of the virus in women with normal cervical cytology are scarce. In this study, ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000024981
更新日期:1999-01-01 00:00:00
abstract::There are two major requirements for potyvirus taxonomy; group-specific criteria and criteria which discriminate between distinct viruses and strains. This review discusses the relative merits in potyvirus taxonomy of molecular parameters, such as gene sequence data, nucleic acid hybridization, coat protein sequence d...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000150211
更新日期:1991-01-01 00:00:00
abstract::A serologic comparison was performed by immune electron microscopy between hepatitis A antigen (related to the MS-1 strain of hepatitis A virus) and a viral isolate, CR69(076), from the stool of a patient in Costa Rica with viral hepatitis. CR69(076) was not antigenically related to hepatitis A antigen but was related...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149486
更新日期:1975-01-01 00:00:00
abstract::XTrack, a rapid, cost-effective diagnostic HIV tropism system, yields predictive results and dissects virus mixtures. It is based on the duplexing of patient samples with selective DNA probes, combined with sequence-based analysis and replicative phenotyping for ambiguous samples. ...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000332003
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVE:Because the determination of the stage of fibrosis depends on rather subjective judgment, more objective parameters are needed. In this study, we followed the long-term outcome, with monitoring of platelet counts, in patients with chronic hepatitis C or liver cirrhosis (LC) who had undergone interferon (IFN) ...
journal_title:Intervirology
pub_type: 临床试验,杂志文章
doi:10.1159/000073209
更新日期:2003-01-01 00:00:00
abstract:OBJECTIVE:Characterization of the phylogeny and diversity of human respiratory syncytial virus (HRSV) genotype ON1 that occurred during its early evolution (within the first 3.5 years since the detection of the first ON1 strains). ON1 strains have a 72-nucleotide-long in-frame duplication within the second hypervariabl...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000382018
更新日期:2015-01-01 00:00:00
abstract::In this study, we analyzed the in vitro expression, potency and longevity of immune responses induced in a Balb/c mouse model by a synthetic HIV-1 GAG gene exhibiting a codon usage that was adapted to that of highly expressed mammalian genes (syngag). In contrast to a vector containing the wild-type (wt) GAG gene, the...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000067919
更新日期:2002-01-01 00:00:00
abstract:OBJECTIVE:Cervical cancer is the third most common malignancy in females. Since the human papillomavirus (HPV) genotype can vary geographically, it is essential to define its genotypic distribution before establishing health care policies and vaccination programs in any given area. The aim of this study was to determin...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000477848
更新日期:2017-01-01 00:00:00
abstract::An RNA virus was isolated from Trichoplusia ni larvae infected with inoculum of the baculovirus of Autographa californica. Physicochemical characterization has revealed a 35-nm virus with a density in CsCl of 1.3 g/cm3 and a sedimentation coefficient of 200S. The virus consisted of 10--15% single-stranded RNA with an ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149040
更新日期:1979-01-01 00:00:00
abstract::Hepatitis C virus (HCV) induces chronic liver disease in hosts which can eventually progresses to liver cirrhosis and hepatocellular carcinoma. However, progression of liver disease is slower in patients with persistently normal levels of alanine aminotransferase (ALT) than in those with active hepatitis. Although dis...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000087264
更新日期:2006-01-01 00:00:00
abstract::Transcription of human T-cell leukemia virus type I is regulated by a viral transactivatior Tax, through the 21-bp sequence in the long terminal repeat (LTR). We found that cellular transcription factor AP-1 (c-Jun/c-Fos heterocomplex) bound to the 21-bp sequence. The binding affinity of the complex increased in propo...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150436
更新日期:1995-01-01 00:00:00