Assembly and accumulation sites of maize mosaic virus in its planthopper vector.

Abstract:

:The morphology, assembly, and accumulation sites of rhabdovirus particles in Peregrinus maidis planthoppers infected with a Hawaiian isolate of maize mosaic virus (MMV) were studied. These particles were usually bullet-shaped, but were sometimes bacilliform, and averaged 234 and 247 nm, respectively, in length and 60 nm in width. They were found in most acini of the principal and accessory salivary glands and in brain, nerve ganglia, leg muscle, foregut, midgut, trachea, epidermis, and fat and connective tissues. In most tissues MMV particles accumulated mainly within intracytoplasmic, dilated cisternae that were connected to the perinuclear space. However, in the salivary glands virus particles accumulated mainly in intercellular and extracellular spaces and were found in secretion vesicles. MMV particles appeared to bud from three types of membranes: (i) inner, and rarely outer, nuclear membranes of cells in most tissues examined; (ii) intracytoplasmic membranes, e.g., endoplasmic reticulum in salivary glands; and (iii) plasma membranes of salivary gland cells and nerve axons. The plasma membrane has not been reported previously as a budding site for plant rhabdoviruses, although it is known as a major assembly site for animal rhabdoviruses.

journal_name

Intervirology

journal_title

Intervirology

authors

Ammar ED,Nault LR

doi

10.1159/000149616

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

33-41

issue

1

eissn

0300-5526

issn

1423-0100

journal_volume

24

pub_type

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