Abstract:
:The 20-nm noninfectious empty envelope particles carrying the hepatitis B surface antigen are secreted in large excess from hepatocytes during a hepatitis B infection. Similar particles produced in cell lines or yeast are an efficient vaccine against hepatitis B virus. We have analyzed the assembly of 20-nm particles using a mutagenesis approach. Specific mutations were introduced into the S gene and the preS region encoding the viral envelope proteins using recombinant DNA techniques. The mutant genes were expressed in cell lines to identify the amino acid residues that are critical for the assembly and the secretion of the 20-nm particles.
journal_name
Intervirologyjournal_title
Intervirologyauthors
Prange R,Mangold CM,Hilfrich R,Streeck REdoi
10.1159/000150410subject
Has Abstractpub_date
1995-01-01 00:00:00pages
16-23issue
1-2eissn
0300-5526issn
1423-0100journal_volume
38pub_type
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