Genotype analysis, using PCR with type-specific primers, of hepatitis B virus isolates from patients coinfected with hepatitis delta virus genotype II from Miyako Island, Japan.

Abstract:

OBJECTIVES:The aims of this study were to determine the hepatitis B virus (HBV) genotypes in hepatitis delta virus (HDV) RNA-positive patients and to characterize the HBV nucleotide sequences that may be found on a distant island of Japan. METHODS:This study included three patients with chronic hepatitis who were positive for hepatitis B surface antigen (enzyme-linked immunosorbent assay; ELISA), HDV antibody (ELISA) and HDV RNA by polymerase chain reaction (PCR). The HBV genotype was determined by nested PCR using type-specific primers. The first-round PCR products from two patients were sequenced, followed by an investigation of nucleotide homology. RESULTS:Viruses from all three patients in this study were classified as HBV genotype B. Comparison with HBV isolates from geographically neighboring regions revealed that the two HBV isolates had 97.9-98.6% identity at the nucleotide level to a Chinese isolate, 98.3-98.6% identity to the Okinawa isolate and 98.6-98.8% identity to a Japanese isolate of genotype B. On phylogenetic analysis, the HBV isolates from the two patients were classified as HBV genotype B. The HBV isolates of cases 1 and 3 clustered in the same group as isolates from the Chinese mainland and Japanese mainland, which are geographically near Miyako Island. CONCLUSION:The HBV isolates coinfected with HDV found on Miyako Island were of genotype B. The PCR method based on genotype-specific primers was useful in determining HBV genotypes.

journal_name

Intervirology

journal_title

Intervirology

authors

Moriyama M,Taira M,Matsumura H,Aoki H,Mikuni M,Kaneko M,Shioda A,Iwaguchi K,Arai S,Ichijima S,Iwasaki H,Tanaka N,Abe K,Arakawa Y

doi

10.1159/000069746

keywords:

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

114-20

issue

2

eissn

0300-5526

issn

1423-0100

pii

69746

journal_volume

46

pub_type

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