Abstract:
OBJECTIVES:The aims of this study were to determine the hepatitis B virus (HBV) genotypes in hepatitis delta virus (HDV) RNA-positive patients and to characterize the HBV nucleotide sequences that may be found on a distant island of Japan. METHODS:This study included three patients with chronic hepatitis who were positive for hepatitis B surface antigen (enzyme-linked immunosorbent assay; ELISA), HDV antibody (ELISA) and HDV RNA by polymerase chain reaction (PCR). The HBV genotype was determined by nested PCR using type-specific primers. The first-round PCR products from two patients were sequenced, followed by an investigation of nucleotide homology. RESULTS:Viruses from all three patients in this study were classified as HBV genotype B. Comparison with HBV isolates from geographically neighboring regions revealed that the two HBV isolates had 97.9-98.6% identity at the nucleotide level to a Chinese isolate, 98.3-98.6% identity to the Okinawa isolate and 98.6-98.8% identity to a Japanese isolate of genotype B. On phylogenetic analysis, the HBV isolates from the two patients were classified as HBV genotype B. The HBV isolates of cases 1 and 3 clustered in the same group as isolates from the Chinese mainland and Japanese mainland, which are geographically near Miyako Island. CONCLUSION:The HBV isolates coinfected with HDV found on Miyako Island were of genotype B. The PCR method based on genotype-specific primers was useful in determining HBV genotypes.
journal_name
Intervirologyjournal_title
Intervirologyauthors
Moriyama M,Taira M,Matsumura H,Aoki H,Mikuni M,Kaneko M,Shioda A,Iwaguchi K,Arai S,Ichijima S,Iwasaki H,Tanaka N,Abe K,Arakawa Ydoi
10.1159/000069746keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
114-20issue
2eissn
0300-5526issn
1423-0100pii
69746journal_volume
46pub_type
杂志文章相关文献
INTERVIROLOGY文献大全abstract::Serological and physical properties of isolated particles as well as reactions of barley cultivars after virus inoculation showed that the yellow disease of barley is caused by different viruses. Results summarized in this paper suggest that the European isolates BaYMV-So and BaYMV-NM are closely related to, or identi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150132
更新日期:1990-01-01 00:00:00
abstract::Bovine adenoviruses (BAVs) are important pathogens causing significant economic losses to the cattle industry. We have been interested in the differences among serotypes of these viruses, particularly in their pathogenicity and host range. As part of our efforts to better understand these viruses, we have determined t...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000024917
更新日期:1998-01-01 00:00:00
abstract::Antibodies of individuals infected with the human immunodeficiency virus type 1 (HIV-1) were used to probe the antigenicity of the HIV-1 transmembrane protein of 41 kD (gp41) by antibody-reactive peptide scanning (Pepscan). Eleven distinct sequential antibody-binding sites were defined by testing reactivity to 339 ove...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150169
更新日期:1990-01-01 00:00:00
abstract:OBJECTIVE:This study sought to investigate the presence of dengue virus binding proteins expressed on the surface of HepG2 cells and to determine if there were serotype specific differences in binding. METHODS:HepG2 cell membrane proteins were extracted and separated by SDS-PAGE, transferred to nitrocellulose membrane...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000080882
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND/AIMS:The present study was launched to systematically characterize the molecular genotypes and quantitative serological biomarkers during the natural course of chronic hepatitis B (CHB) infection in a patient population from Chongqing, China. METHODS:A total of 140 patients were enrolled and grouped accordi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000323524
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVE:The aim was to identify the codon usage bias between the newly identified duck plague virus (DPV) UL35 gene (GenBank accession No. EF643558) and the UL35-like genes of 27 other reference herpesviruses. METHODS:A comparative analysis of the codon usage bias of the 28 herpesviruses was performed by using the C...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000231992
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Infection with tick-borne encephalitis virus (TBEV) causes pathological changes in the central nervous system. However, the possible redox alterations in the infected cells that can contribute to the virus pathogenicity remain unknown. OBJECTIVE:In the current study we explored the ability of TBEV nonstruct...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000452160
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND/AIMS:Adsorption and kinetic parameters, latent period, burst size and burst time, are characteristics of phage/host systems and can be affected by several environmental factors. As only few studies have focused on temperate dairy phages, we characterized these parameters on temperate Lactobacillus paracasei ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000369207
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:Genome-wide sequences of hepatitis B virus strain associated with type B fulminant hepatitis have not been compared with those of acute self-limited hepatitis. We carried out full-length sequencing analysis of viral strains derived from patients with type B acute liver injury. METHODS:Nine acute self-limited...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000110651
更新日期:2007-01-01 00:00:00
abstract::The regulatory regions for transcription and replication of several hepatitis B virus (HBV) genomes from 19 patients having various forms of HBV infection were sequenced. Predominant mutations were found to occur naturally in nucleotide positions 1762 (A to T) and 1764 (G to A) in chronic hepatitis patients and in asy...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150453
更新日期:1995-01-01 00:00:00
abstract::The genetic relationship between two enteroviruses causing the acute hemorrhagic conjunctivitis (AHC) syndrome was examined by the RNA-RNA hybridization technique. Nucleotide sequence homology between the J670/71 strain of enterovirus type 70 (EV70) and the EH 24/70 strain of Singapore epidemic conjunctivitis (SEC) vi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149539
更新日期:1984-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the mutational patterns on the pol gene of the main HIV-1 strain archived in cell genome of 10 chronically infected men according to their clinical and therapeutic history. The genotyping resistance profiles were compared between the first blood plasma available at the time of HIV diagnosis and re...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000472716
更新日期:2016-01-01 00:00:00
abstract::We report a case in which sustained viral response was achieved after switching treatment from pegylated interferon (PEG-IFN) α-2b to α-2a and ribavirin (RBV) in patients with recurrence of hepatitis C virus (HCV) infection after living donor liver transplantation. The patient was a 62-year-old man with liver cirrhosi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000328661
更新日期:2012-01-01 00:00:00
abstract::Prostaglandins exhibit antiviral activity against a wide variety of RNA and DNA viruses. In the present report, we describe the effect of cyclopentenone prostaglandin A(1) (PGA(1)) on Mayaro virus replication in Vero cells. Virus yield was significantly reduced at nontoxic concentrations which did not suppress DNA, RN...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000050069
更新日期:2001-01-01 00:00:00
abstract:OBJECTIVES:To characterize morphological, physicochemical and genomic features of a novel virulent coliphage which was isolated from an engineered Escherichia coli culture and termed engineered E. coli phage (EEP). METHODS AND RESULTS:Electron microscopy revealed that EEP has an icosahedral head (62 nm in diameter) an...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000299063
更新日期:2010-01-01 00:00:00
abstract::6/94 virus, isolated originally from a multiple sclerosis (MS) patient, was compared antigenically with related parainfluenza type 1 strains. These included two Sendai strains of mouse and two Sendai strains of reported human origin as well as the HA2 strain. By standard hemagglutination inhibition (HI) or hemadsorpti...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149875
更新日期:1975-01-01 00:00:00
abstract:BACKGROUND/AIMS:In Zimbabwe, cervical cancer is screened through cytology and visual inspection with acetic acid and cervicography (VIAC). The effectiveness of these methods can be increased if complemented by a human papillomavirus DNA detection tool since most cervical cancer cases are caused by persistent infection ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000502206
更新日期:2019-01-01 00:00:00
abstract::The proposed family Toroviridae is characterized by eveloped, peplomer-bearing particles containing an elongated tubular nucleocapsid with helical symmetry. The capsid may bend into an open torus, conferring a biconcave disk or kidney-shaped morphology to the virion (largest diameter 120-140 nm) or the capsid may be s...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149710
更新日期:1987-01-01 00:00:00
abstract::Healthy common marmosets from two separate colonies, one in England and one in France, were found to have antibodies cross-reacting with Epstein-Barr virus structural antigens (EBV-VCA). All seropositive animals were at least 2 years old. Experimental EBV infection of marmosets of different ages led to seroconversion ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149198
更新日期:1980-01-01 00:00:00
abstract::Influenza PR8 particles resulting from strong treatment with caseinase C are spikeless, devoid of neuraminidase and hemagglutinin 1 and 2 glycopeptides, and contain a Schiff-negative polypeptide of about 13,000 molecular weight which exists as traces in intact virions. Their M-protein polypeptide content is reduced to...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149478
更新日期:1975-01-01 00:00:00
abstract:OBJECTIVES:Kaposi sarcoma-associated herpesvirus (KSHV) glycoprotein B (gB) is expressed on the viral envelope as well as on the cytoplasmic membrane of infected cells. In the current study, we aimed to decipher the impact of membrane-associated gB on adhesion and migration of cells via modulating the expression of cyt...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000487596
更新日期:2017-01-01 00:00:00
abstract::This review summarizes current strategies for the treatment of human cytomegalovirus (CMV) infection/diseases in high-risk patients such as transplant recipients and AIDS patients. Since the major drugs ganciclovir (Cytovene), foscarnet (Foscavir) and cidofovir (Vistide) are frequently associated with severe side effe...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000150568
更新日期:1997-01-01 00:00:00
abstract::The envelope glycoprotein of the mouse mammary tumor virus (MMTV gp52), a useful plasma marker for mammary tumors in mice, was measured in extracellular medium by radioimmunoassay to quantitatively compare gp52 levels released by a C3H MMTV producer and its subcloned B9 nonproducer cell line. Experiments analyzed leve...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150081
更新日期:1989-01-01 00:00:00
abstract::Immunopathologic mechanisms leading to liver tissue injury in hepatitis caused by hepatitis A virus (HAV) were studied in an autologous in vitro model. Data show virus-specific killing by liver-infiltrating T lymphocytes in man and support the hypothesis that hepatocellular damage as well as efficient elimination of v...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150304
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:Many efforts are currently focused on functional treatment of the hepatitis B virus (HBV). This can be done by suppressing the secretion of HBV surface antigen (HBsAg). Scientific communities are very interested in natural products in that respect. OBJECTIVE:Use of root extract of Havachoobe (Onosma dichroa...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000512140
更新日期:2020-12-15 00:00:00
abstract:AIMS:The aim of the present study is the preliminary characterization of an El Tor vibriophage M4 (ATCC 51352-B4). METHODS:We studied the growth characteristics and sustainability of this phage under various stresses like temperature, pH, and UV. The phage morphology and phage genome were also examined using electron ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000485835
更新日期:2017-01-01 00:00:00
abstract:OBJECTIVE:Torque teno virus (TTV) is a ubiquitous virus that is commonly associated with blood transfusion. The main aims of this study were to evaluate the incidence of TTV in polytransfused children with thalassemia and to determine for the first time the prevalent TTV genotypes in Egypt. METHODS:TTV was detected in...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000480507
更新日期:2017-01-01 00:00:00
abstract:OBJECTIVE:The genetic barrier for the evolution of integrase inhibitors (INIs) including raltegravir (RAL), elvitegravir (EVG), and dolutegravir (DTG) resistance was compared between HIV-1 subtypes CRF01_AE and B. METHODS:Analysis of 66 substitutions associated with INI resistance at 41 amino acid positions in 144 nuc...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000336658
更新日期:2012-01-01 00:00:00
abstract::Viroids are minute unencapsidated non-coding circular RNAs known to be present and to cause diseases only in plants. Infections were associated with the occurrence of specific single-stranded RNAs similar in size to miRNAs and endogenous small interfering RNAs, and viroid pathogenicity is suspected to occur through RN...
journal_title:Intervirology
pub_type: 新闻
doi:10.1159/000509212
更新日期:2019-01-01 00:00:00
abstract::Guanidine (GD) inhibited tobacco mosaic virus (TMV) RNA synthesis at two different steps, depending on the concentration of GD used. The time-course of inhibition by 40 mM GD coincided with the time-course of RNA synthesis. However, 10 mM GD inhibited an earlier step that occurred prior to viral RNA synthesis. ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149459
更新日期:1975-01-01 00:00:00