Virus Overlay Protein Binding Assay (VOPBA) reveals serotype specific heterogeneity of dengue virus binding proteins on HepG2 human liver cells.

abstract：:Human skin fibroblasts were previously shown to be resistant to coxsackie B4 virus infection. We have cultured fibroblasts from skin and lung tissues of donors of various ages. By a novel method for direct assay of virus absorption and penetration, we have shown that skin fibroblasts from young fetuses are susceptible...

journal_title：Intervirology

authors： Hornberger E,Plotkin SA

更新日期：1983-01-01 00:00:00

abstract：OBJECTIVES:In the present study, we aimed to determine whether signal-to-cutoff (S/Co) ratios of reactive anti-HCV samples could be used as a basis for avoiding the need for supplemental testing in our study population. METHODS:We analyzed 901 anti-HCV-positive sera from 8 institutions in China. The Ortho VITROS anti-...

journal_title：Intervirology

authors： Zhang K,Wang L,Lin G,Li J

更新日期：2015-01-01 00:00:00

abstract：:Hepatitis C virus (HCV) induces chronic liver disease in hosts which can eventually progresses to liver cirrhosis and hepatocellular carcinoma. However, progression of liver disease is slower in patients with persistently normal levels of alanine aminotransferase (ALT) than in those with active hepatitis. Although dis...

journal_title：Intervirology

authors： Kanto T,Inoue M,Miyazaki M,Itose I,Miyatake H,Sakakibara M,Yakushijin T,Kaimori A,Oki C,Hiramatsu N,Kasahara A,Hayashi N

更新日期：2006-01-01 00:00:00

abstract：:To identify specific environmental, viral, and genetic risk factors for hepatocellular carcinoma (HCC) and the interaction of such factors, we are conducting a prospective study in a high-incidence area of China. Questionnaires were completed and biosamples collected by 60,984 men ages 30-64 years, at study entry. Wit...

journal_title：Intervirology

authors： London WT,Evans AA,McGlynn K,Buetow K,An P,Gao L,Lustbader E,Ross E,Chen G,Shen F

更新日期：1995-01-01 00:00:00

abstract：:A singly enveloped nuclear polyhedrosis virus (SNPV) of the white-marked tussock moth, Orgyia leucostigma, was successfully grown in four continuous cell lines developed from minced neonate larvae of this insect. Level of infection in two cell lines, IPRI-OL-12 and IPRI-OL-13, was 65-90%, but in the other two, IPRI-OL...

journal_title：Intervirology

authors： Sohi SS,Percy J,Arif BM,Cunningham JC

更新日期：1984-01-01 00:00:00

abstract：:The effect of caffeine on cytomegalovirus (CMV) replication in human embryo lung (HEL) and human embryo kidney (HEK) cells was studied. In HEL cultures, nonirradiated CMV yielded up to 6.7 times more plaques in caffeine-treated cultures than in control cultures. Virus growth curves exhibited different profiles, depend...

journal_title：Intervirology

authors： Yamanishi K,Fogel M,Rapp F

更新日期：1978-01-01 00:00:00

abstract：OBJECTIVES:To characterize morphological, physicochemical and genomic features of a novel virulent coliphage which was isolated from an engineered Escherichia coli culture and termed engineered E. coli phage (EEP). METHODS AND RESULTS:Electron microscopy revealed that EEP has an icosahedral head (62 nm in diameter) an...

journal_title：Intervirology

authors： Li S,Liu L,Zhu J,Zou L,Li M,Cong Y,Rao X,Hu X,Zhou Y,Chen Z,Hu F

更新日期：2010-01-01 00:00:00

abstract：:When Vero cells infected with Junín virus are cultured in the presence of 16 mM glucosamine, no infectious virus is produced and no surface antigens or virions are detected. Cytoplasmic viral antigen, however, is synthesized normally. ...

journal_title：Intervirology

authors： Martínez Peralta LA,León ME,Coto CE,Laguens RP

更新日期：1979-01-01 00:00:00

abstract：OBJECTIVE:Sandfly fever phleboviruses are endemic in Mediterranean countries. We report a febrile phlebovirus case in a Greek patient who presented signs of neuroinvasive infection. METHODS:In summer 2010, a 20-year-old male was admitted to hospital with fever and lethargy; he was a resident of central Macedonia, nort...

journal_title：Intervirology

authors： Papa A,Mallias J,Tsergouli K,Markou F,Poulou A,Milidis T

更新日期：2014-01-01 00:00:00

abstract：:The leukocyte migration inhibition (LMI) technique was used to measure the T cell-mediated immune response of Epstein-Barr-virus (EBV)-seropositive human donors to antigens associated with B cell lines of simian origin, transformed by simian EBV-like viruses, Herpesvirus papio (HVP), H. pan, H. gorilla and H. pongo. E...

journal_title：Intervirology

authors： Szigeti R,Rabin H,Timar L,Klein G

更新日期：1986-01-01 00:00:00

abstract：:We examined influenza virus strains of the subtype H3N2 from outbreaks of respiratory diseases in swine herds in Germany. Four different clusters can be distinguished when comparing parts of the HA1 gene from porcine H3N2 isolates analyzed between 1982 and 2001. Comparison between these clusters reveals a bp homology ...

journal_title：Intervirology

authors： Schrader C,Süss J

更新日期：2004-01-01 00:00:00

abstract：:To evaluate the diagnostic value of nucleic-acid-sequence-based amplification (NASBA) for the detection of cytomegalovirus (CMV) infection in transplant recipients, we compared immediate early 1 (IE1) and late pp67 mRNA detection by NASBA with the antigenemia assay, PCR and viral culture in 72 renal transplant (RTx) r...

journal_title：Intervirology

authors： Goossens VJ,Blok MJ,Christiaans MH,van Hooff JP,Sillekens P,Höckerstedt K,Lautenschlager I,Middeldorp JM,Bruggeman CA

更新日期：1999-01-01 00:00:00

abstract：OBJECTIVE:Syncytia formation is the hallmark of the cytopathic effect caused by human respiratory syncytial virus (HRSV), which is the most important viral respiratory pathogen in children. This article reports methodological improvements in primary HRSV isolation and the importance of syncytia formation and mRNA level...

journal_title：Intervirology

authors： Gagliardi TB,Criado MF,Proença-Módena JL,Saranzo AM,Iwamoto MA,de Paula FE,Cardoso RS,Delcaro LS,Silva ML,Câmara AA,Arruda E

更新日期：2017-01-01 00:00:00

abstract：:The Holland strain of anatid herpesvirus (AHV) was 2-to 10-fold more efficiently plaqued under liquid medium or semisolid medium containing methylcellulose than in media containing agar, agarose, or Nobel agar. Virus adsorption was complete in 45 min, with the maximum virus titers obtained under liquid medium at 48 h ...

journal_title：Intervirology

authors： Attanasio R,Olson R,Johnson JC

更新日期：1980-01-01 00:00:00

abstract：:The genetic relationship between two enteroviruses causing the acute hemorrhagic conjunctivitis (AHC) syndrome was examined by the RNA-RNA hybridization technique. Nucleotide sequence homology between the J670/71 strain of enterovirus type 70 (EV70) and the EH 24/70 strain of Singapore epidemic conjunctivitis (SEC) vi...

journal_title：Intervirology

authors： Natori K,Yamazaki S,Miyamura K,Kono R

更新日期：1984-01-01 00:00:00

abstract：:This review briefly summarises the recent knowledge about hantavirus infections and raises particular problems in hantavirus research that need further investigation. The following questions are addressed: (i) are hantaviruses distributed worldwide and what leads to new outbreaks, (ii) what is known about hantavirus e...

journal_title：Intervirology

authors： Ulrich R,Hjelle B,Pitra C,Krüger DH

更新日期：2002-01-01 00:00:00

abstract：OBJECTIVE(S):Chikungunya virus (CHIKV) is a reemerging virus of significant importance that has caused large-scale outbreaks in the countries with a temperate climate. CHIKV causes debilitating arthralgia which can persist for weeks and up to a year. Fibroblast cells are the main target of CHIKV infection. In this stud...

journal_title：Intervirology

authors： Sharma A,Balakathiresan NS,Maheshwari RK

更新日期：2015-01-01 00:00:00

abstract：:The early gene products of simian virus 40 (SV40) transform human fibroblasts, whereas those of the human papovavirus BK (BKV) do not. The early gene products of both SV40 and BKV transform baby rat kidney cells. SV40-BKV chimerics were constructed which were able to transform baby rat kidney cells. Using the SV40-BKV...

journal_title：Intervirology

authors： de Ronde A,Sol CJ,MacDonald ME,Koot M,ter Schegget J,van Strien A,Wouters E,van der Noordaa J

更新日期：1987-01-01 00:00:00

abstract：:The replication of vaccinia virus in human hematopoietic cell lines was studied, to ascertain whether the cell type and the stage of differentiation can influence the outcome of the infection. Lymphocytic NB104 cells and myelogenous leukemic K562 cells can be productively infected with vaccinia. Whereas NB104 cells we...

journal_title：Intervirology

authors： Pogo BG,Lai AC,Holland JG,Friend C

更新日期：1988-01-01 00:00:00

abstract：BACKGROUND:Dengue fever (DF), one of the main emerging virus diseases, also increases in German travellers, due to the increasing number of travellers to tropical regions. METHODS:We determined antibodies against DF virus in a group (n = 149) of long-distance travellers in order to evaluate the risk of acquiring DF. S...

journal_title：Intervirology

authors： Allwinn R,Hofknecht N,Doerr HW

更新日期：2008-01-01 00:00:00

abstract：:A reverse transcriptase, polymerase chain reaction (RT-PCR) procedure was used to amplify a segment of the genome of turkey coronavirus (TCV) spanning portions of the matrix and nucleocapsid (MN) protein genes (approximately 1.1 kb). The MN gene region of three epidemiologically distinct TCV strains (Minnesota, NC95, ...

journal_title：Intervirology

authors： Breslin JJ,Smith LG,Fuller FJ,Guy JS

更新日期：1999-01-01 00:00:00

abstract：OBJECTIVE:To study the anti-herpes simplex virus (HSV) activity of a (1→6)-(1→3)-β-D-glucan isolated from Agaricus brasiliensis fruiting bodies (FR) as well as its chemically sulfated derivative (FR-S). METHODS:The antiherpetic activity and mechanism of action was studied by viral plaque assay applying different metho...

journal_title：Intervirology

authors： Cardozo FT,Camelini CM,Leal PC,Kratz JM,Nunes RJ,Mendonça MM,Simões CM

更新日期：2014-01-01 00:00:00

abstract：:Coinfections with hepatitis B virus (HBV) and HIV are very frequent. Although HBV is a DNA virus, it replicates via reverse transcription like HIV. Structural similarities between the enzymatic pocket of the HBV DNA polymerase and HIV-1 reverse transcriptase are the basis that certain drugs inhibit both enzymes and th...

journal_title：Intervirology

authors： Gürtler LG

更新日期：2014-01-01 00:00:00

abstract：OBJECTIVE:To determine whether liver-derived hepatitis C RNA-containing particles express the E1E2 discontinuous antigenic determinant defined by unique monoclonal antibody (mAb) D32.10 which recognizes three highly conserved segments in E1 (aa297-306) and E2 (aa480-494 and aa613-621) envelope glycoproteins. METHODS:H...

journal_title：Intervirology

authors： Ndongo N,Selliah S,Berthillon P,Raymond VA,Trépo C,Bilodeau M,Petit MA

更新日期：2011-01-01 00:00:00

abstract：AIMS:The aim of the present study is the preliminary characterization of an El Tor vibriophage M4 (ATCC 51352-B4). METHODS:We studied the growth characteristics and sustainability of this phage under various stresses like temperature, pH, and UV. The phage morphology and phage genome were also examined using electron ...

journal_title：Intervirology

authors： Das S,Ghosh AN

更新日期：2017-01-01 00:00:00

abstract：:In order to evaluate the usefulness of cytomegalovirus (CMV) antibody screening during pregnancy, women attending for antenatal care at the Antoine Béclère Hospital (Clamart, France) were prospectively studied during 22 months (1995-1996). Forty-five percent of these women were CMV-seropositive. Twenty suspected or co...

journal_title：Intervirology

authors： Grangeot-Keros L,Simon B,Audibert F,Vial M

更新日期：1998-01-01 00:00:00

abstract：:An open-reading frame designated 1R1, on DNA component 1 of bean golden mosaic geminivirus (BGMV), has been identified as the coat protein gene. A DraI restriction fragment of BGMV DNA1 that includes 1R1 was inserted into the SmaI pKK223-3 expression vector. The 32 kD protein expressed in Escherichia coli cells reacte...

journal_title：Intervirology

authors： Murayama A,Yamaya Y,Ikegami M

更新日期：1991-01-01 00:00:00

abstract：:Marituba virus was purified by rate-zonal sedimentation and equilibrium density centrifugation in sucrose gradients. The buoyant density of virus particles was 1.19 g/ml. Purified virus was dissociated and its proteins were analyzed by SDS-PAGE. Four virus polypeptides were identified and designated L, G1, G2 and N. T...

journal_title：Intervirology

authors： Frugulhetti IC,Soares MC,Rebello MA

更新日期：1983-01-01 00:00:00

abstract：:We report a case in which sustained viral response was achieved after switching treatment from pegylated interferon (PEG-IFN) α-2b to α-2a and ribavirin (RBV) in patients with recurrence of hepatitis C virus (HCV) infection after living donor liver transplantation. The patient was a 62-year-old man with liver cirrhosi...

journal_title：Intervirology

authors： Kawaoka T,Hiraga N,Takahashi S,Takaki S,Tsuge M,Nagaoki Y,Hashimoto Y,Katamura Y,Miki D,Hiramatsu A,Waki K,Imamura M,Kawakami Y,Aikata H,Ochi H,Tashiro H,Ohdan H,Chayama K

更新日期：2012-01-01 00:00:00

abstract：:Echinochloa ragged stunt virus (ERSV) and rice ragged stunt virus (RRSV; Reoviridae) were purified from their respective host plants and disrupted in SDS. Evidence for the double-strandedness of ERSV RNA was obtained. Electrophoresis in 10% polyacrylamide gels resolved the RNAs of each virus into 10 segments, ranging ...

journal_title：Intervirology

authors： Chen CC,Hsu YH,Chen MJ,Chiu RJ

更新日期：1989-01-01 00:00:00