Identification of the coat protein gene of bean golden mosaic geminivirus.

Abstract:

:An open-reading frame designated 1R1, on DNA component 1 of bean golden mosaic geminivirus (BGMV), has been identified as the coat protein gene. A DraI restriction fragment of BGMV DNA1 that includes 1R1 was inserted into the SmaI pKK223-3 expression vector. The 32 kD protein expressed in Escherichia coli cells reacted with antibodies to the BGMV capsid polypeptide and behaved identically to purified capsid protein in western blots.

journal_name

Intervirology

journal_title

Intervirology

authors

Murayama A,Yamaya Y,Ikegami M

doi

10.1159/000150208

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

258-61

issue

4

eissn

0300-5526

issn

1423-0100

journal_volume

32

pub_type

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