Abstract:
:A reverse transcriptase, polymerase chain reaction (RT-PCR) procedure was used to amplify a segment of the genome of turkey coronavirus (TCV) spanning portions of the matrix and nucleocapsid (MN) protein genes (approximately 1.1 kb). The MN gene region of three epidemiologically distinct TCV strains (Minnesota, NC95, Indiana) was amplified, cloned into pUC19, and sequenced. TCV MN gene sequences were compared with published sequences of other avian and mammalian coronaviruses. A high degree of similarity (>90%) was observed between the nucleotide, matrix protein, and nucleocapsid protein sequences of TCV strains and published sequences of infectious bronchitis virus (IBV). The matrix and nucleocapsid protein sequences of TCV had limited homology (<30%) with MN sequences of mammalian coronaviruses. These results demonstrate a close genetic relationship between the avian coronaviruses, IBV and TCV.
journal_name
Intervirologyjournal_title
Intervirologyauthors
Breslin JJ,Smith LG,Fuller FJ,Guy JSdoi
10.1159/000024956keywords:
subject
Has Abstractpub_date
1999-01-01 00:00:00pages
22-9issue
1eissn
0300-5526issn
1423-0100pii
24956journal_volume
42pub_type
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