Sequence analysis of the matrix/nucleocapsid gene region of turkey coronavirus.

Abstract:

:A reverse transcriptase, polymerase chain reaction (RT-PCR) procedure was used to amplify a segment of the genome of turkey coronavirus (TCV) spanning portions of the matrix and nucleocapsid (MN) protein genes (approximately 1.1 kb). The MN gene region of three epidemiologically distinct TCV strains (Minnesota, NC95, Indiana) was amplified, cloned into pUC19, and sequenced. TCV MN gene sequences were compared with published sequences of other avian and mammalian coronaviruses. A high degree of similarity (>90%) was observed between the nucleotide, matrix protein, and nucleocapsid protein sequences of TCV strains and published sequences of infectious bronchitis virus (IBV). The matrix and nucleocapsid protein sequences of TCV had limited homology (<30%) with MN sequences of mammalian coronaviruses. These results demonstrate a close genetic relationship between the avian coronaviruses, IBV and TCV.

journal_name

Intervirology

journal_title

Intervirology

authors

Breslin JJ,Smith LG,Fuller FJ,Guy JS

doi

10.1159/000024956

keywords:

subject

Has Abstract

pub_date

1999-01-01 00:00:00

pages

22-9

issue

1

eissn

0300-5526

issn

1423-0100

pii

24956

journal_volume

42

pub_type

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