Abstract:
:The phosphorylation state of H5R protein was investigated by two-dimensional gel electrophoresis of proteins of BSC-40 cells infected at 32 degrees or 39.5 degrees with vaccinia virus ts mutants of the viral B1R or F10L protein kinase genes. A temperature-dependent increase of underphosphorylated H5R protein (pI 6.8) was demonstrated in the case of the B1R, but not of the F10L gene. The temperature-dependent cytoplasmic location of underphosphorylated H5R protein after infection with the ts mutants of the B1R gene was the consequence of the associated viral DNA replication block. These results show that the B1R protein kinase controls the phosphorylation state of the H5R protein synthesised at the early stage in vaccinia-virus-infected cells.
journal_name
Intervirologyjournal_title
Intervirologyauthors
Beaud G,Beaud Rdoi
10.1159/000025025keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
67-70issue
1eissn
0300-5526issn
1423-0100pii
25025journal_volume
43pub_type
杂志文章相关文献
INTERVIROLOGY文献大全abstract::HIV-2 is responsible for a limited epidemic in West Africa. Around 20% of all infected patients will progress to AIDS, and will need antiretroviral therapy. Unfortunately, antiretrovirals were developed to suppress HIV-1 replication; not all of them are active against HIV-2, e.g. all nonnucleoside reverse transcriptas...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000332025
更新日期:2012-01-01 00:00:00
abstract::We investigated persistent infection of Epstein-Barr virus (EBV)-transformed lymphoblastoid cells with dengue virus using a transmission electron microscope. Most of the infected cells kept an intact morphology with only a few virus particles in the cytoplasm, but without any indication of active viral replication. So...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000050030
更新日期:2001-01-01 00:00:00
abstract::Acute hemorrhagic conjunctivitis (AHC) virus strains isolated in eight different areas during epidemics of AHC were tested for the reproductive capacity at 33, 37 and 39 degrees. All of the 25 strains tested grew better at 33 degrees but restrictively at 39 degrees. The degree of temperature sensitivity varied slightl...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149952
更新日期:1976-01-01 00:00:00
abstract::The WU-virus (WUV), a novel polyomavirus, has recently been recovered from respiratory tract samples. Within a study collective of children with severe lower respiratory tract disease, 3% of the patients tested WUV positive. Viral loads ranged from 5 x 10(2) copies/ml to 1 x 10(4) copies/ml. The WUV genome-positive pa...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000209673
更新日期:2008-01-01 00:00:00
abstract::Dengue and chikungunya fevers are transmitted by the common mosquito vector Aedes and malaria by Anopheles. Concurrent infections are reported due to co-circulation of these pathogens, especially in endemic regions. We report a rare case of triple infection with 3 arthropod-borne pathogens (Plasmodium vivax and the de...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000479430
更新日期:2017-01-01 00:00:00
abstract::5 patients with primary genital herpes simplex virus (HSV) infection were studied prospectively for the production of serum antibody reactivity as measured by the virus micro-neutralization test (NT), micro-solid phase radioimmunometric assay (micro-SPRIA), and antibody-dependent cellular-cytotoxic (ADCC) test. ADCC a...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149320
更新日期:1982-01-01 00:00:00
abstract::An open-reading frame designated 1R1, on DNA component 1 of bean golden mosaic geminivirus (BGMV), has been identified as the coat protein gene. A DraI restriction fragment of BGMV DNA1 that includes 1R1 was inserted into the SmaI pKK223-3 expression vector. The 32 kD protein expressed in Escherichia coli cells reacte...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150208
更新日期:1991-01-01 00:00:00
abstract::Antibodies of individuals infected with the human immunodeficiency virus type 1 (HIV-1) were used to probe the antigenicity of the HIV-1 transmembrane protein of 41 kD (gp41) by antibody-reactive peptide scanning (Pepscan). Eleven distinct sequential antibody-binding sites were defined by testing reactivity to 339 ove...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150169
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND:Dengue virus is spread in tropical areas of the world and is the causative agent of dengue fever and dengue hemorrhagic fever. It is horizontally transmitted to humans by infected Aedes mosquitoes, but it is also able to be vertically or transovarially transmitted to insect progeny. OBJECTIVE:In this work, ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000107272
更新日期:2007-01-01 00:00:00
abstract::Intrauterine viral infections show a wide range of fetal involvement with variable fetal outcome. The histopathologic changes of the placenta during viral infection show a wide spectrum. The most active processes are proliferative with a variety of cells involved. Vascular changes, including inflammatory and obliterat...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000024940
更新日期:1998-01-01 00:00:00
abstract::Dimethyl sulfoxide and twelve related polar compounds, capable of inducing differentiation of murine erythroleukemia cells, markedly inhibited induction of Epstein-Barr virus antigens in virus producer and nonproducer lymphoblastoid cells exposed to 12-O-tetradecanoylphorbol-13-acetate, n-butyric acid, 5-iodo-2'-deoxy...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150088
更新日期:1989-01-01 00:00:00
abstract::Hepatitis B virus (HBV) is one of the major causative agents of hepatocellular carcinoma (HCC) in Southeast Asia where HBV genotype B (HBV/B) and genotype C (HBV/C) are prevalent. Clinical and virological differences were evaluated between HCC patients in Japan and Taiwan, all of whom were infected with HBV/B and HBV/...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000075000
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND/AIMS:Cardiovirus is a genus of viruses belonging to the family Picornaviridae. Here, we used viral metagenomic techniques to detect the viral nucleic acid in the fecal samples from wild rats in Zhenjiang city in China. METHOD:Fecal samples were collected from 20 wild rats and pooled into four sample pools a...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000500555
更新日期:2019-01-01 00:00:00
abstract::Previous reports from this laboratory demonstrated that persistent infections could be established in Jurkat cells with wild-type vaccinia virus (Jvac). The infected cells elicit increased expression of IL-2, IL-2Ralpha and IL-6 but not of IL-1beta nor of interferon-gamma. The persistently infected cells are also capa...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000081734
更新日期:2005-01-01 00:00:00
abstract::The amino acid composition (AAC) of the coat proteins (CPs) of 126 plant viruses or strains were analyzed by stepwise discriminant analysis. The criteria chosen for discrimination were: the structure of virus particles (3 clusters); the mode of of transmission of the viruses (6 clusters); and the grouping of viruses a...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149675
更新日期:1986-01-01 00:00:00
abstract::A BHK/21 cell line persistently infected by an arenavirus is described. During four consecutive passages, 30-45% of the cells showed granular cytoplasmic antigen by indirect immunofluorescence, employing both Argentine hemorrhagic fever convalescent sera and sera from animals immunized with Junin virus. Virus isolated...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149180
更新日期:1980-01-01 00:00:00
abstract::Poliovirus infection of either undifferentiated or differentiated U937 cells produced a decrease in the percentage of cells positive for the surface expression of the CD4, CD11c, CD14, or 8E11 antigens. The number of 4F2 surface molecules per cell increased in infected normal U937 cells, but was unaffected in differen...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150194
更新日期:1991-01-01 00:00:00
abstract:OBJECTIVE:To determine whether liver-derived hepatitis C RNA-containing particles express the E1E2 discontinuous antigenic determinant defined by unique monoclonal antibody (mAb) D32.10 which recognizes three highly conserved segments in E1 (aa297-306) and E2 (aa480-494 and aa613-621) envelope glycoproteins. METHODS:H...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000318886
更新日期:2011-01-01 00:00:00
abstract::Ferret hepatitis E virus (HEV), a novel hepatitis E-like virus, has been identified in ferrets in the Netherlands, Japan, and the US. To determine whether ferret HEV transmits to other animals, we inoculated laboratory rats (Wistar), nude rats (Long-Evans-rnu/rnu), and cynomolgus monkeys with ferret HEV (F4351) by int...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000373891
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVES:The potential risk of accidental infection by hantaviruses in a clinical or research laboratory necessitates special precautionary measures. A biosafety program must address handling and disposal of infectious materials as well as appropriate virus inactivation or depletion procedures to permit necessary fur...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000084603
更新日期:2005-01-01 00:00:00
abstract::Among patients undergoing maintenance hemodialysis, a high prevalence of hepatitis C virus (HCV) infection can be observed. In a prospective study, sera of 273 patients were examined for the presence of HCV infection by serological tests and by PCR. Thirty-five patients (12.8%) were HCV antibody positive, and in 31 of...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150558
更新日期:1997-01-01 00:00:00
abstract::CMV-transformed cell lines demonstrated a greater level of H-ras RNA (7.5- to 9.5-fold) relative to the level for H-ras in parental cells. Nuclear run off assays showed that the RNA levels for the H-ras gene were regulated at the level of transcriptional initiation. The increased RNA levels for H-ras correlated with t...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150396
更新日期:1994-01-01 00:00:00
abstract::The complete nucleotide sequence of rice dwarf phytoreovirus genome segment 2 (S2) was determined to be 3,512 nucleotides long with one open reading frame initiating at nucleotide 15 and terminating at nucleotide 3363. The encoded polypeptide was predicted to have 1,116 residues with a relative molecular weight of 123...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150348
更新日期:1994-01-01 00:00:00
abstract::The mainstay of therapy for patients with chronic hepatitis B is inhibition of the replicative cycle of hepatitis B virus (HBV) in hepatocytes. Antiviral agents have different potencies. The measurement of HBV DNA in serum can monitor the potency of these therapeutic agents. Viral breakthrough may occur during therapy...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000074990
更新日期:2003-01-01 00:00:00
abstract::XTrack, a rapid, cost-effective diagnostic HIV tropism system, yields predictive results and dissects virus mixtures. It is based on the duplexing of patient samples with selective DNA probes, combined with sequence-based analysis and replicative phenotyping for ambiguous samples. ...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000332003
更新日期:2012-01-01 00:00:00
abstract::Scrapie brain homogenate was mixed with mouse peritoneal macrophages in vitro. After 2 h of incubation at 37 degrees, a portion of the scrapie infectivity was associated with macrophages. In contrast, very little infectivity was associated with kidney cells that had been exposed to scrapie brain homogenate. After incu...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149241
更新日期:1981-01-01 00:00:00
abstract::Human skin fibroblasts were previously shown to be resistant to coxsackie B4 virus infection. We have cultured fibroblasts from skin and lung tissues of donors of various ages. By a novel method for direct assay of virus absorption and penetration, we have shown that skin fibroblasts from young fetuses are susceptible...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149360
更新日期:1983-01-01 00:00:00
abstract::Viroids are minute unencapsidated non-coding circular RNAs known to be present and to cause diseases only in plants. Infections were associated with the occurrence of specific single-stranded RNAs similar in size to miRNAs and endogenous small interfering RNAs, and viroid pathogenicity is suspected to occur through RN...
journal_title:Intervirology
pub_type: 新闻
doi:10.1159/000509212
更新日期:2019-01-01 00:00:00
abstract::Virions of maize rayado fino virus (MRFV) were purified and two major capsid proteins (ca. Mr 29,000 and 22,000) were resolved by SDS-PAGE. When the two major capsid proteins were isolated from gels and compared by one-dimensional peptide mapping after digestion with Staphylococcus aureus V-8 protease, indistinguishab...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149664
更新日期:1986-01-01 00:00:00
abstract::The replication of vaccinia virus in human hematopoietic cell lines was studied, to ascertain whether the cell type and the stage of differentiation can influence the outcome of the infection. Lymphocytic NB104 cells and myelogenous leukemic K562 cells can be productively infected with vaccinia. Whereas NB104 cells we...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150024
更新日期:1988-01-01 00:00:00