Temperature-dependent phosphorylation state of the H5R protein synthesised at the early stage of infection in cells infected with vaccinia virus ts mutants of the B1R and F10L protein kinases.

Abstract:

:The phosphorylation state of H5R protein was investigated by two-dimensional gel electrophoresis of proteins of BSC-40 cells infected at 32 degrees or 39.5 degrees with vaccinia virus ts mutants of the viral B1R or F10L protein kinase genes. A temperature-dependent increase of underphosphorylated H5R protein (pI 6.8) was demonstrated in the case of the B1R, but not of the F10L gene. The temperature-dependent cytoplasmic location of underphosphorylated H5R protein after infection with the ts mutants of the B1R gene was the consequence of the associated viral DNA replication block. These results show that the B1R protein kinase controls the phosphorylation state of the H5R protein synthesised at the early stage in vaccinia-virus-infected cells.

journal_name

Intervirology

journal_title

Intervirology

authors

Beaud G,Beaud R

doi

10.1159/000025025

keywords:

subject

Has Abstract

pub_date

2000-01-01 00:00:00

pages

67-70

issue

1

eissn

0300-5526

issn

1423-0100

pii

25025

journal_volume

43

pub_type

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