Abstract:
:A BHK/21 cell line persistently infected by an arenavirus is described. During four consecutive passages, 30-45% of the cells showed granular cytoplasmic antigen by indirect immunofluorescence, employing both Argentine hemorrhagic fever convalescent sera and sera from animals immunized with Junin virus. Virus isolated from the cells killed suckling mice but not adult mice and protected guinea pigs against further challenge with the virulent prototype strain of Junin virus. Neutralization tests showed that the virus isolated from the cells was neutralized by anti-Junin virus antisera. The usefulness of this cell line in rapid immunofluorescent serological procedures is described.
journal_name
Intervirologyjournal_title
Intervirologyauthors
Carballal G,Cossio PM,Rabinovich A,Oubiña J,Arana RMdoi
10.1159/000149180subject
Has Abstractpub_date
1980-01-01 00:00:00pages
173-9issue
3-4eissn
0300-5526issn
1423-0100journal_volume
14pub_type
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