Abstract:
:We have studied the effect of gamma-interferon (IFN-gamma) treatment on the transcription of viral and c-myc oncogenes in bovine papillomavirus type 1 (BPV-1)-transformed mouse fibroblast cell lines. Upon IFN-gamma treatment, viral transcripts always decreased in cell lines containing episomal BPV-1 DNA, while the effect was variable in cell lines containing integrated BPV-1 DNA. Two series of tumour cell lines established by in vivo passage of B6B71 (episomal) and B6B31-J (integrated) cells via nude mice (NuTu A) into immunocompetent C57BL/6 mice (NuTuA B6Tul) also showed a decrease and an increase, respectively, in viral transcripts upon IFN-gamma treatment. IFN-gamma also reduced c-myc transcription in all cell lines derived from tumours, but increased it in the transformed cell cultures. There was selection of c-myc oncogene transcripts in all the tumour-derived cell lines as compared to their transformed cell cultures. Cycloheximide treatment increased both viral and c-myc gene transcripts 3- to 20-fold in all cell lines. These results imply that IFN-gamma produced locally by immunological mechanisms may influence the expression of Papillomavirus oncogenes, and the response to IFN-gamma treatment may change when the viral DNA is integrated into the host genome.
journal_name
Intervirologyjournal_title
Intervirologyauthors
Agrawal R,Pelkonen J,Mäntyjärvi RAdoi
10.1159/000150320subject
Has Abstractpub_date
1993-01-01 00:00:00pages
44-52issue
1eissn
0300-5526issn
1423-0100journal_volume
36pub_type
杂志文章相关文献
INTERVIROLOGY文献大全abstract::The use of glutaraldehyde (GA)-treated chicken red blood cells (RBC) for purification of Sendai virions by adsorption and elution was found to be advantageous over the use of untreated RBC. Biologic and enzymatic activities and structural components of the purified virions were compared with those of the original viri...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000148872
更新日期:1977-01-01 00:00:00
abstract::Coinfections with hepatitis B virus (HBV) and HIV are very frequent. Although HBV is a DNA virus, it replicates via reverse transcription like HIV. Structural similarities between the enzymatic pocket of the HBV DNA polymerase and HIV-1 reverse transcriptase are the basis that certain drugs inhibit both enzymes and th...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000360942
更新日期:2014-01-01 00:00:00
abstract::The large tumour antigen (TAg) of simian virus 40 is the main regulatory protein in viral replication. We have investigated possible functional roles for the N-terminal domain of TAg while defining the epitopes of antibodies binding within that region. The epitopes recognised by eight monoclonal anti-TAg antibodies we...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000024910
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND:Arboviruses co-circulating within a population that are transmitted by the same vector have the potential to cause coinfections. Coinfections with dengue virus (DENV), Zika virus (ZIKV), and chikungunya virus (CHIKV) have been occurring in Brazil, but it is not well-understood how human responses vary during...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000510223
更新日期:2020-01-01 00:00:00
abstract::The amino acid sequences of the virion proteins of 7 definitive tobamoviruses have been compared. The results show that the proteins may be arranged unequivocally in a single dendrogram which, by analogy with similar comparisons of other sets of homologous proteins, probably reflects their phylogeny. The magnitude of ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149169
更新日期:1980-01-01 00:00:00
abstract::Despite the significant medical advances which have taken place in the last decades, acute diarrhoea cases remain a public health issue of major significance, with gastroenteritis agents being associated with severe symptoms in adults and high morbidity in infants and children. Regarding rotaviruses, while children ar...
journal_title:Intervirology
pub_type: 新闻
doi:10.1159/000502007
更新日期:2019-01-01 00:00:00
abstract::The genetic relationship between two enteroviruses causing the acute hemorrhagic conjunctivitis (AHC) syndrome was examined by the RNA-RNA hybridization technique. Nucleotide sequence homology between the J670/71 strain of enterovirus type 70 (EV70) and the EH 24/70 strain of Singapore epidemic conjunctivitis (SEC) vi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149539
更新日期:1984-01-01 00:00:00
abstract:OBJECTIVES:Data on drug-resistant mutations (DRMs) in HIV-1-infected therapy-naïve children are scarce. The aim of this study was to determine the HIV-1 subtype distribution and the prevalence of DRMs in therapy-naïve HIV-1-infected children who received routine care at the University Hospital Düsseldorf, Düsseldorf, G...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000477811
更新日期:2016-01-01 00:00:00
abstract::Influenza PR8 particles resulting from strong treatment with caseinase C are spikeless, devoid of neuraminidase and hemagglutinin 1 and 2 glycopeptides, and contain a Schiff-negative polypeptide of about 13,000 molecular weight which exists as traces in intact virions. Their M-protein polypeptide content is reduced to...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149478
更新日期:1975-01-01 00:00:00
abstract::To evaluate the diagnostic value of nucleic-acid-sequence-based amplification (NASBA) for the detection of cytomegalovirus (CMV) infection in transplant recipients, we compared immediate early 1 (IE1) and late pp67 mRNA detection by NASBA with the antigenemia assay, PCR and viral culture in 72 renal transplant (RTx) r...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000053974
更新日期:1999-01-01 00:00:00
abstract::When Vero cells infected with Junín virus are cultured in the presence of 16 mM glucosamine, no infectious virus is produced and no surface antigens or virions are detected. Cytoplasmic viral antigen, however, is synthesized normally. ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149032
更新日期:1979-01-01 00:00:00
abstract::The complete nucleotide sequence of rice dwarf phytoreovirus genome segment 2 (S2) was determined to be 3,512 nucleotides long with one open reading frame initiating at nucleotide 15 and terminating at nucleotide 3363. The encoded polypeptide was predicted to have 1,116 residues with a relative molecular weight of 123...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150348
更新日期:1994-01-01 00:00:00
abstract:OBJECTIVE:Giant viruses of the Megavirales order have been neglected in the literature because they are removed from samples during viral purification for viral metagenomic studies. Isolation via amoeba coculture has low efficiency and is extremely time-consuming. Thus, our objective was to improve Megavirales detectio...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000354566
更新日期:2013-01-01 00:00:00
abstract::Hepatitis B virus (HBV) core antigen (HBcAg) detected by the peroxidase-antiperoxidase technique was present in the nucleus and cytoplasm of some infected hepatocytes but only in the cytoplasm of other hepatocytes. When cells expressing HBcAg were examined by in situ hybridization for the presence of HBV DNA, the intr...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149646
更新日期:1985-01-01 00:00:00
abstract::In order to assess the risk of transmission of viral diseases during floods, the viral burden in flooded areas of the city of Dresden (Germany) in August 2002 was investigated. Water samples were collected from 9 sampling sites and tested for the presence of 11 enteric viral pathogens. As a control, water samples from...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000095157
更新日期:2006-01-01 00:00:00
abstract:OBJECTIVE:To investigate the effect of HIV Tat protein on Bcl-2 expression in human monocytes, and observe apoptosis of Tat-stimulated monocytes induced by TNF-alpha-related apoptosis-induced ligand (TRAIL). METHODS:Western blot was used to detect Bcl-2 expression in monocytes stimulated by HIV Tat protein, and Annexi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000100565
更新日期:2007-01-01 00:00:00
abstract:OBJECTIVES:The potential risk of accidental infection by hantaviruses in a clinical or research laboratory necessitates special precautionary measures. A biosafety program must address handling and disposal of infectious materials as well as appropriate virus inactivation or depletion procedures to permit necessary fur...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000084603
更新日期:2005-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to attain molecular knowledge of human papillomavirus type 18 (HPV18) by sequencing the whole genome of HPV18 isolated from Thai women at various clinical stages of disease progression. METHOD:Our group analyzed 9 samples of whole-genome HPV18 in infected women ranging from normal t...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000274977
更新日期:2010-01-01 00:00:00
abstract::The envelope glycoprotein of the mouse mammary tumor virus (MMTV gp52), a useful plasma marker for mammary tumors in mice, was measured in extracellular medium by radioimmunoassay to quantitatively compare gp52 levels released by a C3H MMTV producer and its subcloned B9 nonproducer cell line. Experiments analyzed leve...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150081
更新日期:1989-01-01 00:00:00
abstract::An amyloid-like isoform of a 33- to 34-kD glycoprotein, termed as the scrapie prion protein (PrPsc), plays a critical role in transmissible spongiform encephalopathies of animals and humans. It has even been suggested to present the responsible infectious agent. This protein is a posttranslationally modified form of t...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150457
更新日期:1995-01-01 00:00:00
abstract::Membrane particles possessing receptor activity for influenza virions have been reconstituted following solubilization of human erythrocyte membranes with octylglucoside (OG) and fractionation on a DEAE-cellulose column. Fractions that eluted with 1.5 M NaCl yielded, after removal of OG, reconstituted membrane particl...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149334
更新日期:1983-01-01 00:00:00
abstract:OBJECTIVE:Syncytia formation is the hallmark of the cytopathic effect caused by human respiratory syncytial virus (HRSV), which is the most important viral respiratory pathogen in children. This article reports methodological improvements in primary HRSV isolation and the importance of syncytia formation and mRNA level...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000480014
更新日期:2017-01-01 00:00:00
abstract::The use of bleach (hypochlorite) as a disinfectant for drug injection equipment in the intravenous-drug-using population was recommended early in the HIV-1/AIDS epidemic. Epidemiological studies have challenged the use of bleach as an effective measure to prevent HIV-1 transmission. However, in vitro HIV-1 coculture s...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000025015
更新日期:2000-01-01 00:00:00
abstract:OBJECTIVE:Sandfly fever phleboviruses are endemic in Mediterranean countries. We report a febrile phlebovirus case in a Greek patient who presented signs of neuroinvasive infection. METHODS:In summer 2010, a 20-year-old male was admitted to hospital with fever and lethargy; he was a resident of central Macedonia, nort...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000363505
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND/AIMS:The present study was launched to systematically characterize the molecular genotypes and quantitative serological biomarkers during the natural course of chronic hepatitis B (CHB) infection in a patient population from Chongqing, China. METHODS:A total of 140 patients were enrolled and grouped accordi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000323524
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the exposure of piglets to enteroviruses-G (EV-G) through the presence of antibodies in their serum. METHODS:Serum samples were obtained from the vena cava of 10 piglets at 9 weeks of age and again 39 days later (day 39). They were tested using an immunoassay based on...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000449058
更新日期:2016-01-01 00:00:00
abstract::Early hepatocellular carcinoma (HCC) as defined by the Liver Cancer Study Group of Japan would correspond to high-grade dysplastic nodules with small foci of HCC in the majority of cases, using the classification system proposed by the International Working Party. A large number of early HCCs were revealed to be hypo-...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000078473
更新日期:2004-01-01 00:00:00
abstract:OBJECTIVES:In the present study, we aimed to determine whether signal-to-cutoff (S/Co) ratios of reactive anti-HCV samples could be used as a basis for avoiding the need for supplemental testing in our study population. METHODS:We analyzed 901 anti-HCV-positive sera from 8 institutions in China. The Ortho VITROS anti-...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000441474
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:Human Salivirus (SalV) has been associated with gastroenteritis on all continents. METHODS:This paper presents the real-time RT-PCR assay for the detection of SalV in clinical fecal samples collected from 192 hospitalized children with acute gastroenteritis in Piedmont, Italy. RESULTS:The most commonly dete...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000490568
更新日期:2018-01-01 00:00:00
abstract::Dengue and chikungunya fevers are transmitted by the common mosquito vector Aedes and malaria by Anopheles. Concurrent infections are reported due to co-circulation of these pathogens, especially in endemic regions. We report a rare case of triple infection with 3 arthropod-borne pathogens (Plasmodium vivax and the de...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000479430
更新日期:2017-01-01 00:00:00