Purification of Sendai virions with glutaraldehyde-treated red blood cells.

abstract：OBJECTIVE:The aim of this study is to investigate the molecular mechanism of hepatocellular carcinoma (HCC) development induced by hepatitis B virus X protein (HBx). METHODS:We previously established a H7402-X cell line that constitutively expresses HBx protein. In the present study, H7402-X gene expression profiles a...

journal_title：Intervirology

authors： Ye L,Dong N,Wang Q,Xu Z,Cai N,Wang H,Zhang X

更新日期：2008-01-01 00:00:00

abstract：:Human skin fibroblasts were previously shown to be resistant to coxsackie B4 virus infection. We have cultured fibroblasts from skin and lung tissues of donors of various ages. By a novel method for direct assay of virus absorption and penetration, we have shown that skin fibroblasts from young fetuses are susceptible...

journal_title：Intervirology

authors： Hornberger E,Plotkin SA

更新日期：1983-01-01 00:00:00

abstract：:The critical point dried (CPD) whole cell technique was applied to the study of the morphogenesis and morphology of rabies virus and vesicular stomatitis virus (VSV) in mouse neuroblastoma and baby hamster kidney (BHK) cells. With the stereoscopic technique, progeny viruses at the cell surface and within the cytoplasm...

journal_title：Intervirology

authors： Iwasaki Y

更新日期：1978-01-01 00:00:00

abstract：OBJECTIVES:The potential risk of accidental infection by hantaviruses in a clinical or research laboratory necessitates special precautionary measures. A biosafety program must address handling and disposal of infectious materials as well as appropriate virus inactivation or depletion procedures to permit necessary fur...

journal_title：Intervirology

authors： Kraus AA,Priemer C,Heider H,Kruger DH,Ulrich R

更新日期：2005-01-01 00:00:00

abstract：:Cultures of human cells transformed by SV40 were found to release infectious virus, even after several passages in vitro. Virus shedding by these cultures did not depend on propagation of virus from cell to cell, as it was not affected by anti-SV40 antiserum that could effectively block virus propagation in acutely in...

journal_title：Intervirology

authors： Lomax CA,Thirion JP,Bourgaux-Ramoisy D

更新日期：1978-01-01 00:00:00

abstract：BACKGROUND:Many efforts are currently focused on functional treatment of the hepatitis B virus (HBV). This can be done by suppressing the secretion of HBV surface antigen (HBsAg). Scientific communities are very interested in natural products in that respect. OBJECTIVE:Use of root extract of Havachoobe (Onosma dichroa...

journal_title：Intervirology

authors： Mohebbi A,Azadi F,Hashemi MM,Askari FS,Razzaghi N

更新日期：2020-12-15 00:00:00

abstract：:The leukocyte migration inhibition (LMI) technique was used to measure the T cell-mediated immune response of Epstein-Barr-virus (EBV)-seropositive human donors to antigens associated with B cell lines of simian origin, transformed by simian EBV-like viruses, Herpesvirus papio (HVP), H. pan, H. gorilla and H. pongo. E...

journal_title：Intervirology

authors： Szigeti R,Rabin H,Timar L,Klein G

更新日期：1986-01-01 00:00:00

abstract：:Despite frequent statements to the contrary, there are good animal models for AIDS. In this review, we summarize the properties of one of the most useful animal models: infection of rhesus monkeys with simian immunodeficiency virus (SIV). The SIVs are an extensive group of HIV-related lentiviruses of nonhuman primates...

journal_title：Intervirology

authors： Desrosiers RC,Ringler DJ

更新日期：1989-01-01 00:00:00

abstract：:Examination of newborn mice, inoculated intraperitoneally with high doses of scrapie virus, revealed that the virus could not be reisolated from their tissues after about 1 week following inoculation, until almost 1 year later. The inoculum was rapidly removed and was not detectable, although the animals became latent...

journal_title：Intervirology

authors： Hotchin J,Sikora E,Baker F

更新日期：1983-01-01 00:00:00

abstract：OBJECTIVE:Sandfly fever phleboviruses are endemic in Mediterranean countries. We report a febrile phlebovirus case in a Greek patient who presented signs of neuroinvasive infection. METHODS:In summer 2010, a 20-year-old male was admitted to hospital with fever and lethargy; he was a resident of central Macedonia, nort...

journal_title：Intervirology

authors： Papa A,Mallias J,Tsergouli K,Markou F,Poulou A,Milidis T

更新日期：2014-01-01 00:00:00

abstract：:Epstein-Barr virus (EBV) is a common herpesvirus that may cause asymptomatic infection or various diseases, such as mononucleosis, lymphoproliferative disorders and several cancers. Our objective was to estimate the prevalence of EBV among patients hospitalized in "Luigi Vanvitelli" University Hospital in the last 10 ...

journal_title：Intervirology

authors： Franci G,Crudele V,Della Rocca MT,Melardo C,Chianese A,Finamore E,Bencivenga F,Astorri R,Vitiello M,Galdiero E,Galdiero M

更新日期：2019-01-01 00:00:00

abstract：:We report a case in which sustained viral response was achieved after switching treatment from pegylated interferon (PEG-IFN) α-2b to α-2a and ribavirin (RBV) in patients with recurrence of hepatitis C virus (HCV) infection after living donor liver transplantation. The patient was a 62-year-old man with liver cirrhosi...

journal_title：Intervirology

authors： Kawaoka T,Hiraga N,Takahashi S,Takaki S,Tsuge M,Nagaoki Y,Hashimoto Y,Katamura Y,Miki D,Hiramatsu A,Waki K,Imamura M,Kawakami Y,Aikata H,Ochi H,Tashiro H,Ohdan H,Chayama K

更新日期：2012-01-01 00:00:00

abstract：:Monoclonal antibodies were used to study measles virus (Hallé strain) infections. The distribution of virus antigens in acutely and persistently infected cells was examined by immunofluorescence, and the synthesis of the virus polypeptides in acutely infected cells was measured after immunoprecipitation and analysis i...

journal_title：Intervirology

authors： Giraudon P,Gerald C,Wild TF

更新日期：1984-01-01 00:00:00

abstract：:Guanidine (GD) inhibited tobacco mosaic virus (TMV) RNA synthesis at two different steps, depending on the concentration of GD used. The time-course of inhibition by 40 mM GD coincided with the time-course of RNA synthesis. However, 10 mM GD inhibited an earlier step that occurred prior to viral RNA synthesis. ...

journal_title：Intervirology

authors： Dawson WO

更新日期：1975-01-01 00:00:00

abstract：OBJECTIVE:Torque teno virus (TTV) is a ubiquitous virus that is commonly associated with blood transfusion. The main aims of this study were to evaluate the incidence of TTV in polytransfused children with thalassemia and to determine for the first time the prevalent TTV genotypes in Egypt. METHODS:TTV was detected in...

journal_title：Intervirology

authors： Hassuna NA,Naguib E,Abdel-Fatah M,Mousa SMO

更新日期：2017-01-01 00:00:00

abstract：:The complete nucleotide sequence of rice dwarf phytoreovirus genome segment 2 (S2) was determined to be 3,512 nucleotides long with one open reading frame initiating at nucleotide 15 and terminating at nucleotide 3363. The encoded polypeptide was predicted to have 1,116 residues with a relative molecular weight of 123...

journal_title：Intervirology

authors： Uyeda I,Suda N,Yamada N,Kudo H,Murao K,Suga H,Kimura I,Shikata E,Kitagawa Y,Kusano T

更新日期：1994-01-01 00:00:00

abstract：:Despite the significant medical advances which have taken place in the last decades, acute diarrhoea cases remain a public health issue of major significance, with gastroenteritis agents being associated with severe symptoms in adults and high morbidity in infants and children. Regarding rotaviruses, while children ar...

journal_title：Intervirology

authors： Vontas A,Hadjicristodoulou C,Krikelis V,Petinaki E

更新日期：2019-01-01 00:00:00

abstract：:Influenza PR8 particles resulting from strong treatment with caseinase C are spikeless, devoid of neuraminidase and hemagglutinin 1 and 2 glycopeptides, and contain a Schiff-negative polypeptide of about 13,000 molecular weight which exists as traces in intact virions. Their M-protein polypeptide content is reduced to...

journal_title：Intervirology

authors： Reginster M,Rentier B,Dierickx L

更新日期：1975-01-01 00:00:00

abstract：OBJECTIVES:To characterize morphological, physicochemical and genomic features of a novel virulent coliphage which was isolated from an engineered Escherichia coli culture and termed engineered E. coli phage (EEP). METHODS AND RESULTS:Electron microscopy revealed that EEP has an icosahedral head (62 nm in diameter) an...

journal_title：Intervirology

authors： Li S,Liu L,Zhu J,Zou L,Li M,Cong Y,Rao X,Hu X,Zhou Y,Chen Z,Hu F

更新日期：2010-01-01 00:00:00

abstract：:In this article, a statistical score method for comparison of partial genomic regions in their representatives of full-length genome of hepatitis E virus (HEV) for genotyping was developed. The critical values of the statistics for hypothesis testing were generated through Monte Carlo simulations. It was found that fr...

journal_title：Intervirology

authors： Xun P,Chen F,Dong C,Qian G,Lai D,Meng J

更新日期：2007-01-01 00:00:00

abstract：:An amyloid-like isoform of a 33- to 34-kD glycoprotein, termed as the scrapie prion protein (PrPsc), plays a critical role in transmissible spongiform encephalopathies of animals and humans. It has even been suggested to present the responsible infectious agent. This protein is a posttranslationally modified form of t...

journal_title：Intervirology

authors： Tanji K,Saeki K,Matsumoto Y,Takeda M,Hirasawa K,Doi K,Matsumoto Y,Onodera T

更新日期：1995-01-01 00:00:00

abstract：:Previous reports documented the recovery of a DNA virus from a patient with posttransfusion non-A-G hepatitis and named TT virus (TTV). Although the virus was initially detected as a causative agent of hepatitis, there is doubt about its pathogenicity. The aim of this study was to clarify the relationship between TTV ...

journal_title：Intervirology

authors： Suzuki F,Chayama K,Tsubota A,Akuta N,Someya T,Kobayashi M,Suzuki Y,Saitoh S,Arase Y,Ikeda K,Kumada H

更新日期：2001-01-01 00:00:00

abstract：:Acute hemorrhagic conjunctivitis (AHC) virus strains isolated in eight different areas during epidemics of AHC were tested for the reproductive capacity at 33, 37 and 39 degrees. All of the 25 strains tested grew better at 33 degrees but restrictively at 39 degrees. The degree of temperature sensitivity varied slightl...

journal_title：Intervirology

authors： Miyamura K,Sasagawa A,Tajiri E,Kono R

更新日期：1976-01-01 00:00:00

abstract：:The replication of vaccinia virus in human hematopoietic cell lines was studied, to ascertain whether the cell type and the stage of differentiation can influence the outcome of the infection. Lymphocytic NB104 cells and myelogenous leukemic K562 cells can be productively infected with vaccinia. Whereas NB104 cells we...

journal_title：Intervirology

authors： Pogo BG,Lai AC,Holland JG,Friend C

更新日期：1988-01-01 00:00:00

abstract：:Ferret hepatitis E virus (HEV), a novel hepatitis E-like virus, has been identified in ferrets in the Netherlands, Japan, and the US. To determine whether ferret HEV transmits to other animals, we inoculated laboratory rats (Wistar), nude rats (Long-Evans-rnu/rnu), and cynomolgus monkeys with ferret HEV (F4351) by int...

journal_title：Intervirology

authors： Li TC,Yoshizaki S,Ami Y,Suzaki Y,Yang T,Takeda N,Takaji W

更新日期：2015-01-01 00:00:00

abstract：:Despite decades of research, no aetiologic factor(s) for human breast cancer has been identified and the search for a causal agent has all but been abandoned during the past thirty years. Over 60 years ago, it was demonstrated that breast tumours in mice are caused by an oncornavirus, murine mammary tumour virus (MMTV...

journal_title：Intervirology

authors： Mant C,Hodgson S,Hobday R,D'Arrigo C,Cason J

更新日期：2004-01-01 00:00:00

abstract：OBJECTIVE:The specific effect of rna interference on the replication of porcine hemagglutinating encephalomyelitis virus (phe-cov) was explored. METHODS:Four species of small interfering RNA (siRNA), targeting different regions of the PHE-CoV spike glycoprotein and replicase polyprotein genes, were prepared by in vitr...

journal_title：Intervirology

authors： Lan Y,Lu H,Zhao K,He W,Chen K,Wang G,Song D,Gao F

更新日期：2012-01-01 00:00:00

abstract：OBJECTIVE:In this study we established a mouse brain injection system to detect infectious dengue-2 virus produced from the full-length RNA transcripts. METHODS:In vitro transcription was used to synthesize full-length dengue-2 virus RNA from the plasmid pRS424FLDEN2NGC, which was intracerebrally injected into the 6-d...

journal_title：Intervirology

authors： Lee YR,Huang KJ,Lei HY,Chen SH,Lin YS,Yeh TM,Liu HS

更新日期：2005-01-01 00:00:00

abstract：:The envelope glycoprotein of the mouse mammary tumor virus (MMTV gp52), a useful plasma marker for mammary tumors in mice, was measured in extracellular medium by radioimmunoassay to quantitatively compare gp52 levels released by a C3H MMTV producer and its subcloned B9 nonproducer cell line. Experiments analyzed leve...

journal_title：Intervirology

authors： Ritzi EM,Walthall EB

更新日期：1989-01-01 00:00:00

abstract：:The reactivity of sera from patients with cervical cancer with the E7 protein of human papilloma virus type 16 (HPV16) was estimated using a novel non-radioactive immunoprecipitation assay and four established protein- and peptide-based immunoassays. Six of 14 sera from patients with cervical cancer and 1 of 10 sera f...

journal_title：Intervirology

authors： Malcolm K,Meschede W,Pawlita M,Koutsky LA,Frazer IH

更新日期：2000-01-01 00:00:00