Abstract:
OBJECTIVE:In this study we established a mouse brain injection system to detect infectious dengue-2 virus produced from the full-length RNA transcripts. METHODS:In vitro transcription was used to synthesize full-length dengue-2 virus RNA from the plasmid pRS424FLDEN2NGC, which was intracerebrally injected into the 6-day-old suckling mice (ICR strains). Engineered dengue-2 viruses were detected in the brain sections using immunohistochemistry staining. RT-PCR followed by restriction endonuclease BstEII digestion was used to confirm the mosquito C6/36 cells cocultured with the mouse brain extract. RESULTS:The mice inoculated with the full-length dengue-2 viral RNA transcript showed paralysis symptoms and died between day 10 and 13 postinjection. The dengue-2 virus-specific antigens (E, Core and NS1) were detected in all the brain and part of the liver sections of the paralyzed mice by immunohistochemistry staining, indicating the existence of dengue-2 virus in these tissues of the suckling mice. The viruses detected in the brains of suckling mice were indeed infectious, which was further confirmed by coculturing mosquito C6/36 cells with the brain extract of the injected mice. CONCLUSIONS:We developed an in vivo approach to detect and produce engineered dengue viruses with infectivity from the full-length plasmid cDNA. This suckling mice system will also aid in screening the infectious viruses that are created by site-directed mutagenesis and is useful for the studies of dengue virus gene function and pathogenesis in the host.
journal_name
Intervirologyjournal_title
Intervirologyauthors
Lee YR,Huang KJ,Lei HY,Chen SH,Lin YS,Yeh TM,Liu HSdoi
10.1159/000081744keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
161-6issue
2-3eissn
0300-5526issn
1423-0100pii
81744journal_volume
48pub_type
杂志文章相关文献
INTERVIROLOGY文献大全abstract:OBJECTIVE:The open reading frame 8 (ORF8) within the short unique (U(s)) region of the bovine herpesvirus 1 (BHV-1) genome was predicted to encode a protein with homology to U(s)9 protein of other alpha herpesviruses. The aim of this study is to identify the protein encoded by the U(s) ORF8 and to examine the effect of...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000063236
更新日期:2002-01-01 00:00:00
abstract::Ferret hepatitis E virus (HEV), a novel hepatitis E-like virus, has been identified in ferrets in the Netherlands, Japan, and the US. To determine whether ferret HEV transmits to other animals, we inoculated laboratory rats (Wistar), nude rats (Long-Evans-rnu/rnu), and cynomolgus monkeys with ferret HEV (F4351) by int...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000373891
更新日期:2015-01-01 00:00:00
abstract::Coinfections with hepatitis B virus (HBV) and HIV are very frequent. Although HBV is a DNA virus, it replicates via reverse transcription like HIV. Structural similarities between the enzymatic pocket of the HBV DNA polymerase and HIV-1 reverse transcriptase are the basis that certain drugs inhibit both enzymes and th...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000360942
更新日期:2014-01-01 00:00:00
abstract::An open-reading frame designated 1R1, on DNA component 1 of bean golden mosaic geminivirus (BGMV), has been identified as the coat protein gene. A DraI restriction fragment of BGMV DNA1 that includes 1R1 was inserted into the SmaI pKK223-3 expression vector. The 32 kD protein expressed in Escherichia coli cells reacte...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150208
更新日期:1991-01-01 00:00:00
abstract::The early gene products of simian virus 40 (SV40) transform human fibroblasts, whereas those of the human papovavirus BK (BKV) do not. The early gene products of both SV40 and BKV transform baby rat kidney cells. SV40-BKV chimerics were constructed which were able to transform baby rat kidney cells. Using the SV40-BKV...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150019
更新日期:1987-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the mutational patterns on the pol gene of the main HIV-1 strain archived in cell genome of 10 chronically infected men according to their clinical and therapeutic history. The genotyping resistance profiles were compared between the first blood plasma available at the time of HIV diagnosis and re...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000472716
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVES:To characterize morphological, physicochemical and genomic features of a novel virulent coliphage which was isolated from an engineered Escherichia coli culture and termed engineered E. coli phage (EEP). METHODS AND RESULTS:Electron microscopy revealed that EEP has an icosahedral head (62 nm in diameter) an...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000299063
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:Dengue virus is spread in tropical areas of the world and is the causative agent of dengue fever and dengue hemorrhagic fever. It is horizontally transmitted to humans by infected Aedes mosquitoes, but it is also able to be vertically or transovarially transmitted to insect progeny. OBJECTIVE:In this work, ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000107272
更新日期:2007-01-01 00:00:00
abstract::Biochemical studies on human coronavirus, strain 229E, indicate that the RNA is present in the virion in association with protein as a ribonucleoprotein (RNP) complex. Morphological studies have revealed that this nucleocapsid is probably a continuous helical RNP. ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149474
更新日期:1975-01-01 00:00:00
abstract::Virus-associated DNA polymerase activity has recently been proposed for the detection of human cytomegalovirus (HCMV) in urine, a method that should allow rapid and quantitative determination of the viral load. In this report, the virus-associated DNA polymerase activity recovered from the urine of a group of patients...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150530
更新日期:1996-01-01 00:00:00
abstract::Rotavirus is the most common cause of severe diarrhoea worldwide, affecting over 125 million young children every year in developing countries. The present study is a part of ongoing childhood diarrhoeal surveillance to determine the strain diversity of rotaviruses prevalent in Andaman and Nicobar Islands. From Octobe...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000342219
更新日期:2013-01-01 00:00:00
abstract::Four tetraploid cell lines were derived from the Epstein-Barr virus (EBV)-carrying, nonproducer Burkitt lymphoma line Raji after colcemide treatment. EBV early antigen (EA) was induced by P3HR-1 virus superinfection and by iododeoxyuridine treatment. Inducibility was reduced in all tetraploid lines compared to the nea...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000148973
更新日期:1978-01-01 00:00:00
abstract::Marituba virus was purified by rate-zonal sedimentation and equilibrium density centrifugation in sucrose gradients. The buoyant density of virus particles was 1.19 g/ml. Purified virus was dissociated and its proteins were analyzed by SDS-PAGE. Four virus polypeptides were identified and designated L, G1, G2 and N. T...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149383
更新日期:1983-01-01 00:00:00
abstract::Interferon induction in BSC-1 cells by measles virus isolates previously found to be interferon inducing (IF+) or interferon noninducing (IF-) was studied in different cell types. Lack of induction by the IF- viruses was not due to an inhibitory factor since BSC-1 cells coinfected with IF+ and IF- stocks produced inte...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149274
更新日期:1981-01-01 00:00:00
abstract::A whitefly-transmitted virus from Venezuela, potato yellow mosaic virus, has been propagated, isolated and characterized as a member of the Geminivirus group. The virus was transmitted to several species of Nicotiana and to Petunia hybrida by mechanical inoculation, and to potato and tomato plants by grafting. Purifie...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150042
更新日期:1988-01-01 00:00:00
abstract::The complete nucleotide sequence of rice dwarf phytoreovirus genome segment 2 (S2) was determined to be 3,512 nucleotides long with one open reading frame initiating at nucleotide 15 and terminating at nucleotide 3363. The encoded polypeptide was predicted to have 1,116 residues with a relative molecular weight of 123...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150348
更新日期:1994-01-01 00:00:00
abstract::9-beta-D-Arabinofuranosyladenine (ara A) was shown to inhibit a specific step of tobacco mosaic virus (TMV) multiplication. The time course of the ara A-sensitive function occurred 'late' coinciding with the period of viral RNA and protein synthesis. When treatment began after viral RNA synthesis was established, 1.0 ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149694
更新日期:1986-01-01 00:00:00
abstract:OBJECTIVE:To clarify the value of contrast-enhanced harmonic ultrasonography (US) with Sonazoid, a second-generation US contrast agent, in the differential diagnosis of liver tumors compared to dynamic CT. METHODS:A total of 249 hepatic nodules in 214 patients were studied; these included 177 hepatocellular carcinomas...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000122600
更新日期:2008-01-01 00:00:00
abstract::The 20-nm noninfectious empty envelope particles carrying the hepatitis B surface antigen are secreted in large excess from hepatocytes during a hepatitis B infection. Similar particles produced in cell lines or yeast are an efficient vaccine against hepatitis B virus. We have analyzed the assembly of 20-nm particles ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150410
更新日期:1995-01-01 00:00:00
abstract::Transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhea virus (PEDV) are similar coronaviruses, causing diseases characterized by vomiting, diarrhea, and death from severe dehydration in piglets. Thus, they have caused huge losses to the swine-breeding industry worldwide. Nowadays, they are easily tra...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000492424
更新日期:2018-01-01 00:00:00
abstract::Nasal natural killer (NK)/T cell lymphoma is a peculiar lymphoma with a unique immunophenotype. Etiologically, in 1990, the authors first demonstrated the presence of Epstein-Barr virus (EBV) genomes and their products in this lymphoma. EBV-specific cytotoxic T lymphocytes (CTL) are very important in controlling the l...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000106462
更新日期:2007-01-01 00:00:00
abstract::An RNA virus was isolated from Trichoplusia ni larvae infected with inoculum of the baculovirus of Autographa californica. Physicochemical characterization has revealed a 35-nm virus with a density in CsCl of 1.3 g/cm3 and a sedimentation coefficient of 200S. The virus consisted of 10--15% single-stranded RNA with an ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149040
更新日期:1979-01-01 00:00:00
abstract::Human cytomegalovirus (CMV) has devised numerous means of escaping immune surveillance. The CMV genome encodes at least 4 genes involved in downregulating surface expression of HLA class I molecules. In addition, it sequesters CC chemokines, induces Fc receptors, interferes with induction of HLA class II antigens, and...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000053964
更新日期:1999-01-01 00:00:00
abstract::A seroepidemiological survey of the human syncytial (foamy) virus was done by means of an indirect immunofluorescence test on 1,717 sera from nine different Pacific island territories. The specificity of the reaction was verified by neutralization tests. The study indicated that the virus is ubiquitous in this part of...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149112
更新日期:1980-01-01 00:00:00
abstract::Dimethyl sulfoxide and twelve related polar compounds, capable of inducing differentiation of murine erythroleukemia cells, markedly inhibited induction of Epstein-Barr virus antigens in virus producer and nonproducer lymphoblastoid cells exposed to 12-O-tetradecanoylphorbol-13-acetate, n-butyric acid, 5-iodo-2'-deoxy...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150088
更新日期:1989-01-01 00:00:00
abstract:OBJECTIVE:The aim was to identify the codon usage bias between the newly identified duck plague virus (DPV) UL35 gene (GenBank accession No. EF643558) and the UL35-like genes of 27 other reference herpesviruses. METHODS:A comparative analysis of the codon usage bias of the 28 herpesviruses was performed by using the C...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000231992
更新日期:2009-01-01 00:00:00
abstract:OBJECTIVES:Chikungunya virus (CHIKV) is an alphavirus belonging to the Togaviridae family. Alphaviruses cause a chronic non-cytopathic infection in mosquito cells, while they develop a highly cytopathic infection in cells originating from various vertebrates. In this study, we compared the cytopathic effect (CPE) induc...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000339985
更新日期:2013-01-01 00:00:00
abstract::To evaluate the diagnostic value of nucleic-acid-sequence-based amplification (NASBA) for the detection of cytomegalovirus (CMV) infection in transplant recipients, we compared immediate early 1 (IE1) and late pp67 mRNA detection by NASBA with the antigenemia assay, PCR and viral culture in 72 renal transplant (RTx) r...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000053974
更新日期:1999-01-01 00:00:00
abstract:OBJECTIVE:To study the anti-herpes simplex virus (HSV) activity of a (1→6)-(1→3)-β-D-glucan isolated from Agaricus brasiliensis fruiting bodies (FR) as well as its chemically sulfated derivative (FR-S). METHODS:The antiherpetic activity and mechanism of action was studied by viral plaque assay applying different metho...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000365194
更新日期:2014-01-01 00:00:00
abstract::Cancer of the cervix is the most common malignant tumor among women in Africa and, in particular, Senegal. Studies of the prevalence of human papilloma virus (HPV) infection in Africa have mainly focused on carcinomas. Data on the presence of the virus in women with normal cervical cytology are scarce. In this study, ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000024981
更新日期:1999-01-01 00:00:00