PCR-RFLP-detected human papilloma virus infection in a group of senegalese women attending an STD clinic and identification of a new HPV-68 subtype.

Abstract:

:Cancer of the cervix is the most common malignant tumor among women in Africa and, in particular, Senegal. Studies of the prevalence of human papilloma virus (HPV) infection in Africa have mainly focused on carcinomas. Data on the presence of the virus in women with normal cervical cytology are scarce. In this study, 158 cytologically normal women who had been referred to the 'Institut Pasteur de Dakar' (Senegal) for various genital complaints were investigated for the presence of HPV on exfoliated cells by PCR-RFLP. HPV was detected in 13.9% of cases. Oncogenic type HPV 16 was the most common type (40.9%), followed by HPV 53 and HPV 58, both detected in 13.6% of cases. Mixed HPV infections were present in 13. 6% of the subjects. Only HPVs belonging to the intermediate-high risk group were detected. These data suggest the need for careful cytological control of patients. A PCR-HPV fragment (GA115) possessing an original RFLP pattern was isolated. After sequencing, it showed a nucleotide homology of 97.1% with HPV 68 and should therefore be considered a new HPV 68 subtype. The use of PCR-RFLP strategy enables detection and typing of all known and as yet unknown genital HPVs. Variant and subtype classification of HPV types identified by oligonucleotide probe methods may need to be refined, especially for less prevalent HPVs and in areas where little information on HPV prevalence is available. More studies are needed to characterize satisfactorily the epidemiology of HPV in Africa.

journal_name

Intervirology

journal_title

Intervirology

authors

Astori G,Beltrame A,Pipan C,Raphenon G,Botta GA

doi

10.1159/000024981

keywords:

subject

Has Abstract

pub_date

1999-01-01 00:00:00

pages

221-7

issue

4

eissn

0300-5526

issn

1423-0100

pii

int42221

journal_volume

42

pub_type

杂志文章
  • Shedding of virus-free MMTV gp52 by nonproducer mammary tumor cells: a source of tumor marker in the presence and absence of dexamethasone.

    abstract::The envelope glycoprotein of the mouse mammary tumor virus (MMTV gp52), a useful plasma marker for mammary tumors in mice, was measured in extracellular medium by radioimmunoassay to quantitatively compare gp52 levels released by a C3H MMTV producer and its subcloned B9 nonproducer cell line. Experiments analyzed leve...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000150081

    authors: Ritzi EM,Walthall EB

    更新日期:1989-01-01 00:00:00

  • Mixed infection with two tobamoviruses: the formation of particles containing the coat protein messenger RNAs of either virus.

    abstract::Plants mixedly infected with the U2 strain of tobacco mosaic virus (T2MV) and sunnhemp mosaic virus (SHMV) and grown at 35 degrees, yield particles of the same modal lengths (300 and 40 nm) as those found in plants singly infected with SHMV, but not in plants infected with T2MV, which yield only the long particles. At...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149973

    authors: Skotnicki A,Gibbs A,Shaw DC

    更新日期:1976-01-01 00:00:00

  • Impairment of the M-protein and unmasking of a superficial type-specific antigen by proteolytic treatment of influenza A virions with preservation of host-specific antigenicity.

    abstract::Influenza PR8 particles resulting from strong treatment with caseinase C are spikeless, devoid of neuraminidase and hemagglutinin 1 and 2 glycopeptides, and contain a Schiff-negative polypeptide of about 13,000 molecular weight which exists as traces in intact virions. Their M-protein polypeptide content is reduced to...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149478

    authors: Reginster M,Rentier B,Dierickx L

    更新日期:1975-01-01 00:00:00

  • Long-term outcome, with monitoring of platelet counts, in patients with chronic hepatitis C and liver cirrhosis after interferon therapy.

    abstract:OBJECTIVE:Because the determination of the stage of fibrosis depends on rather subjective judgment, more objective parameters are needed. In this study, we followed the long-term outcome, with monitoring of platelet counts, in patients with chronic hepatitis C or liver cirrhosis (LC) who had undergone interferon (IFN) ...

    journal_title:Intervirology

    pub_type: 临床试验,杂志文章

    doi:10.1159/000073209

    authors: Moriyama M,Matsumura H,Aoki H,Shimizu T,Nakai K,Saito T,Yamagami H,Shioda A,Kaneko M,Goto I,Tanaka N,Arakawa Y

    更新日期:2003-01-01 00:00:00

  • Rotavirus genotypes in Costa Rica, Nicaragua, Honduras and the Dominican Republic.

    abstract::In this study, 574 stool samples from children with gastroenteritis were obtained from different hospitals in Costa Rica, Honduras, Nicaragua and the Dominican Republic during 2005-2006. Diarrhea stool samples were analyzed for rotavirus by ELISA and typed by the RT-PCR-based method. Unusual strains were detected: G1P...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000317288

    authors: Bourdett-Stanziola L,Ortega-Barria E,Espinoza F,Bucardo F,Jimenez C,Ferrera A

    更新日期:2010-01-01 00:00:00

  • Membrane-Associated Kaposi Sarcoma-Associated Herpesvirus Glycoprotein B Promotes Cell Adhesion and Inhibits Migration of Cells via Upregulating IL-1β and TNF-α.

    abstract:OBJECTIVES:Kaposi sarcoma-associated herpesvirus (KSHV) glycoprotein B (gB) is expressed on the viral envelope as well as on the cytoplasmic membrane of infected cells. In the current study, we aimed to decipher the impact of membrane-associated gB on adhesion and migration of cells via modulating the expression of cyt...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000487596

    authors: Hussein HAM,Abdel-Raouf UM,Akula SM

    更新日期:2017-01-01 00:00:00

  • Prediction of efficacy of interferon treatment of chronic hepatitis C and occurrence of HCC after interferon treatment by a new classification.

    abstract::One hundred and twenty-one chronic hepatitis C patients were administered interferon (IFN) and divided into two groups: 31 complete responders (CR) with prolonged HCV-RNA negative 1 year after treatment and 90 non-complete responders (non-CR), including partial responders (PR) with transiently negative HCV-RNA and non...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000082095

    authors: Kim KI,Sasase N,Taniguchi M,Mita K,Kim SR,Tanaka K,Hayashi Y

    更新日期:2005-01-01 00:00:00

  • Chikungunya Virus Infection Alters Expression of MicroRNAs Involved in Cellular Proliferation, Immune Response and Apoptosis.

    abstract:OBJECTIVE(S):Chikungunya virus (CHIKV) is a reemerging virus of significant importance that has caused large-scale outbreaks in the countries with a temperate climate. CHIKV causes debilitating arthralgia which can persist for weeks and up to a year. Fibroblast cells are the main target of CHIKV infection. In this stud...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000441309

    authors: Sharma A,Balakathiresan NS,Maheshwari RK

    更新日期:2015-01-01 00:00:00

  • Differential diagnosis of hepatic tumors: value of contrast-enhanced harmonic sonography using the newly developed contrast agent, Sonazoid.

    abstract:OBJECTIVE:To clarify the value of contrast-enhanced harmonic ultrasonography (US) with Sonazoid, a second-generation US contrast agent, in the differential diagnosis of liver tumors compared to dynamic CT. METHODS:A total of 249 hepatic nodules in 214 patients were studied; these included 177 hepatocellular carcinomas...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000122600

    authors: Hatanaka K,Kudo M,Minami Y,Ueda T,Tatsumi C,Kitai S,Takahashi S,Inoue T,Hagiwara S,Chung H,Ueshima K,Maekawa K

    更新日期:2008-01-01 00:00:00

  • Characterization of human Influenza Viruses in Lebanon during 2010-2011 and 2011-2012 post-pandemic seasons.

    abstract:OBJECTIVE:To genetically characterize human influenza viruses and their susceptibilities to antivirals during two post-pandemic seasons in Lebanon. METHODS:Influenza virus was isolated from nasopharyngeal swabs that were obtained from patients with influenza-like illness during 2010-2012 and further analyzed both phen...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000365758

    authors: Zaraket H,Dapat C,Ghanem S,Ali Z,Lteif M,Kondo H,Dapat IC,Saito K,Kayali G,Suzuki H,Dbaibo G,Saito R

    更新日期:2014-01-01 00:00:00

  • Testing of CpG-optimized protein and DNA vaccines against the hepatitis B virus in chimpanzees for immunogenicity and protection from challenge.

    abstract::Despite the existence for some time of effective prophylactic vaccines, hepatitis B virus (HBV) infection remains an important global concern. Improvements on existing vaccines could be beneficial, especially in situations where it is desirable or necessary to induce protective immunity more rapidly or with fewer dose...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000089375

    authors: Payette PJ,Ma X,Weeratna RD,McCluskie MJ,Shapiro M,Engle RE,Davis HL,Purcell RH

    更新日期:2006-01-01 00:00:00

  • Effect of antiretroviral HIV therapy on hepatitis B virus replication and pathogenicity.

    abstract::Coinfections with hepatitis B virus (HBV) and HIV are very frequent. Although HBV is a DNA virus, it replicates via reverse transcription like HIV. Structural similarities between the enzymatic pocket of the HBV DNA polymerase and HIV-1 reverse transcriptase are the basis that certain drugs inhibit both enzymes and th...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000360942

    authors: Gürtler LG

    更新日期:2014-01-01 00:00:00

  • Two domains within the early coding region of SV40 involved in the transformation of human fibroblasts.

    abstract::The early gene products of simian virus 40 (SV40) transform human fibroblasts, whereas those of the human papovavirus BK (BKV) do not. The early gene products of both SV40 and BKV transform baby rat kidney cells. SV40-BKV chimerics were constructed which were able to transform baby rat kidney cells. Using the SV40-BKV...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000150019

    authors: de Ronde A,Sol CJ,MacDonald ME,Koot M,ter Schegget J,van Strien A,Wouters E,van der Noordaa J

    更新日期:1987-01-01 00:00:00

  • In vitro interaction of scrapie agent and mouse peritoneal macrophages.

    abstract::Scrapie brain homogenate was mixed with mouse peritoneal macrophages in vitro. After 2 h of incubation at 37 degrees, a portion of the scrapie infectivity was associated with macrophages. In contrast, very little infectivity was associated with kidney cells that had been exposed to scrapie brain homogenate. After incu...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149241

    authors: Carp RI,Callahan SM

    更新日期:1981-01-01 00:00:00

  • Effect of 2-thiouracil on RNA and protein synthesis in synchronous and asynchronous infections of tobacco mosaic virus.

    abstract::Examination of the effect of 2-thiouracil on tobacco mosaic virus (TMV) RNA and protein synthesis in synchronous and asynchronous systems of replication in tobacco leaves demonstrated that 2-thiouracil does not prevent synthesis of TMV RNA, as suggested by previous studies, but inhibits some earlier function. When add...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149351

    authors: Dawson WO,Grantham GL

    更新日期:1983-01-01 00:00:00

  • Cytomegalovirus glycoprotein B genotypes in Mexican children and women.

    abstract:OBJECTIVE:Cytomegalovirus (CMV) is widely distributed and constitutes the main cause of congenital infections worldwide. CMV transmission during pregnancy represents one of the major impacts of this virus on public health. This study aimed at assessing glycoprotein B (gB) CMV genotypes in Mexican children and pregnant ...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000373922

    authors: Gonzalez-Sanchez HM,Alvarado-Hernandez DL,Guerra-Palomares S,Garcia-Sepulveda CA,Noyola DE

    更新日期:2015-01-01 00:00:00

  • Screening cellular proteins binding to the core region of hepatitis C virus RNA genome with digoxin-labeled nucleic acids.

    abstract:OBJECTIVE:To screen and identify cellular proteins binding to the core region of hepatitis C virus (HCV) RNA genome. METHODS:The plasmid pHCV core was constructed to generate in vitro transcripts of the core region of HCV RNA genome. Ultraviolet (UV) cross-linking experiment and competition analysis were performed to ...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000105443

    authors: Su HX,Xu DZ,Zhang YH,Men K,Zhao XN,Li D,Zhang L,Zhang JX,Yan YP

    更新日期:2007-01-01 00:00:00

  • Molecular virology of hepatitis B virus and targets for antiviral intervention.

    abstract::The members of the viral family Hepadnaviridae comprise one of the smallest enveloped DNA viruses and cause acute and chronic infections in mammals and birds, leading to large virus and antigen loads in the blood. They have a restricted host range and depend on differentiated hepatocytes for replication. Hepatitis B v...

    journal_title:Intervirology

    pub_type: 杂志文章,评审

    doi:10.1159/000360946

    authors: Glebe D,König A

    更新日期:2014-01-01 00:00:00

  • The two capsid proteins of maize rayado fino virus contain common peptide sequences.

    abstract::Virions of maize rayado fino virus (MRFV) were purified and two major capsid proteins (ca. Mr 29,000 and 22,000) were resolved by SDS-PAGE. When the two major capsid proteins were isolated from gels and compared by one-dimensional peptide mapping after digestion with Staphylococcus aureus V-8 protease, indistinguishab...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149664

    authors: Falk BW,Tsai JH

    更新日期:1986-01-01 00:00:00

  • HIV-2 in rhesus monkeys: serological, virological and clinical results.

    abstract::Diseases induced by animal retroviruses are not considered to be good models for the human acquired immunodeficiency syndrome (AIDS) at present. The lack of an animal model for the human immunodeficiency virus (HIV) infection presents a main problem in the complete understanding of the pathogenesis of HIV-mediated dis...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000150125

    authors: Dormont D,Livartowski J,Chamaret S,Guetard D,Henin D,Levagueresse R,van de Moortelle PF,Larke B,Gourmelon P,Vazeux R

    更新日期:1989-01-01 00:00:00

  • Antigenic variation among parainfluenza type 1 (Sendai) viruses: analysis of 6/94 virus.

    abstract::6/94 virus, isolated originally from a multiple sclerosis (MS) patient, was compared antigenically with related parainfluenza type 1 strains. These included two Sendai strains of mouse and two Sendai strains of reported human origin as well as the HA2 strain. By standard hemagglutination inhibition (HI) or hemadsorpti...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149875

    authors: Lief FS,Loh W,Meulen VT,Koprowski H

    更新日期:1975-01-01 00:00:00

  • Biological properties of Junin virus proteins. I. Identification of the immunogenic glycoprotein.

    abstract::Purified Junin virus was disrupted by nonionic detergent treatment (Triton X-100), and subviral components were separated by ultracentrifugation. Only the soluble fraction, containing all of the major glycoprotein (mol.wt. 38,000), induced the formation of neutralizing antibodies in laboratory animals and protected th...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149136

    authors: Cresta B,Padula P,de Martinez Segovia M

    更新日期:1980-01-01 00:00:00

  • Mycosis fungoides in European Russia: no antibodies to human T cell leukemia virus type I structural proteins, but virus-like sequences in blood and saliva.

    abstract:OBJECTIVE:Mycosis fungoides (MF) is the most frequent form of cutaneous T cell lymphoma (CTCL). Human T cell leukemia virus type 1 (HTLV-1) involvement in MF progression is a matter of debate. The goal of the investigation was to search for HTLV-1 markers in a group of MF patients from a nonendemic area to HTLV-1. MAT...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000086063

    authors: Morozov VA,Syrtsev AV,Ellerbrok H,Nikolaeva EV,Bavykin AS,Pauli G

    更新日期:2005-01-01 00:00:00

  • Electron microscopic study of persistent dengue virus infection: analysis using a cell line persistently infected with Dengue-2 virus.

    abstract::We investigated persistent infection of Epstein-Barr virus (EBV)-transformed lymphoblastoid cells with dengue virus using a transmission electron microscope. Most of the infected cells kept an intact morphology with only a few virus particles in the cytoplasm, but without any indication of active viral replication. So...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000050030

    authors: Takasaki T,Takada K,Kurane I

    更新日期:2001-01-01 00:00:00

  • Seroepidemiology of human syncytial virus: antibody prevalence in the Pacific.

    abstract::A seroepidemiological survey of the human syncytial (foamy) virus was done by means of an indirect immunofluorescence test on 1,717 sera from nine different Pacific island territories. The specificity of the reaction was verified by neutralization tests. The study indicated that the virus is ubiquitous in this part of...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149112

    authors: Loh PC,Matsuura F,Mizumoto C

    更新日期:1980-01-01 00:00:00

  • Alphavirus vectors: applications for DNA vaccine production and gene expression.

    abstract::Replication-deficient alphavirus vectors have been developed for efficient high-level transgene expression. The broad host range of alphaviruses has allowed infection of a wide variety of mammalian cell lines and primary cultures. Particularly, G protein-coupled receptors have been expressed at high levels and subject...

    journal_title:Intervirology

    pub_type: 杂志文章,评审

    doi:10.1159/000053992

    authors: Lundstrom K

    更新日期:2000-01-01 00:00:00

  • Havachoobe (Onosma dichroanthum Boiss) Root Extract Decreases the Hepatitis B Virus Surface Antigen Secretion in the PLC/PRF/5 Cell Line.

    abstract:BACKGROUND:Many efforts are currently focused on functional treatment of the hepatitis B virus (HBV). This can be done by suppressing the secretion of HBV surface antigen (HBsAg). Scientific communities are very interested in natural products in that respect. OBJECTIVE:Use of root extract of Havachoobe (Onosma dichroa...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000512140

    authors: Mohebbi A,Azadi F,Hashemi MM,Askari FS,Razzaghi N

    更新日期:2020-12-15 00:00:00

  • Antiherpetic mechanism of a sulfated derivative of Agaricus brasiliensis fruiting bodies polysaccharide.

    abstract:OBJECTIVE:To study the anti-herpes simplex virus (HSV) activity of a (1→6)-(1→3)-β-D-glucan isolated from Agaricus brasiliensis fruiting bodies (FR) as well as its chemically sulfated derivative (FR-S). METHODS:The antiherpetic activity and mechanism of action was studied by viral plaque assay applying different metho...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000365194

    authors: Cardozo FT,Camelini CM,Leal PC,Kratz JM,Nunes RJ,Mendonça MM,Simões CM

    更新日期:2014-01-01 00:00:00

  • Chikungunya virus induces a more moderate cytopathic effect in mosquito cells than in mammalian cells.

    abstract:OBJECTIVES:Chikungunya virus (CHIKV) is an alphavirus belonging to the Togaviridae family. Alphaviruses cause a chronic non-cytopathic infection in mosquito cells, while they develop a highly cytopathic infection in cells originating from various vertebrates. In this study, we compared the cytopathic effect (CPE) induc...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000339985

    authors: Li YG,Siripanyaphinyo U,Tumkosit U,Noranate N,A-nuegoonpipat A,Tao R,Kurosu T,Ikuta K,Takeda N,Anantapreecha S

    更新日期:2013-01-01 00:00:00

  • Immunoelectrophoretic identification and purification of herpes simplex virus antigens released from infected cells in tissue culture.

    abstract::Proteins released from herpes simplex virus type 1 (HSV-1)-and type 2 (HSV-2)-infected HEp-2 cells have been characterized by the crossed immunoelectrophoretic technique. Both HSV type-common and type-specific antigens were found in the tissue culture medium 24 h after infection. Antigen Ag-6, an HSV-1-specific antige...

    journal_title:Intervirology

    pub_type: 杂志文章

    doi:10.1159/000149020

    authors: Norrild B,Vestergaard BF

    更新日期:1979-01-01 00:00:00