Biological properties of Junin virus proteins. I. Identification of the immunogenic glycoprotein.

abstract：:Adult Hartley guinea pigs infected with guinea pig cytomegalovirus (CMV) develop a mononucleosis syndrome with a brief viremia, splenomegaly, lymphadenopathy, and peripheral lymphocytosis with circulating atypical lymphocytes. The present study used this experimental model to evaluate in vivo the therapeutic efficacy ...

journal_title：Intervirology

authors： Lucia HL,Griffith BP,Hsiung GD

更新日期：1984-01-01 00:00:00

abstract：:In this study, we analyzed the in vitro expression, potency and longevity of immune responses induced in a Balb/c mouse model by a synthetic HIV-1 GAG gene exhibiting a codon usage that was adapted to that of highly expressed mammalian genes (syngag). In contrast to a vector containing the wild-type (wt) GAG gene, the...

journal_title：Intervirology

authors： Bojak A,Wild J,Deml L,Wagner R

更新日期：2002-01-01 00:00:00

abstract：:Monoclonal antibodies were used to study measles virus (Hallé strain) infections. The distribution of virus antigens in acutely and persistently infected cells was examined by immunofluorescence, and the synthesis of the virus polypeptides in acutely infected cells was measured after immunoprecipitation and analysis i...

journal_title：Intervirology

authors： Giraudon P,Gerald C,Wild TF

更新日期：1984-01-01 00:00:00

abstract：:Guanidine (GD) inhibited tobacco mosaic virus (TMV) RNA synthesis at two different steps, depending on the concentration of GD used. The time-course of inhibition by 40 mM GD coincided with the time-course of RNA synthesis. However, 10 mM GD inhibited an earlier step that occurred prior to viral RNA synthesis. ...

journal_title：Intervirology

authors： Dawson WO

更新日期：1975-01-01 00:00:00

abstract：OBJECTIVES:Fluctuation profile has been observed in chronic hepatitis B patients who are untreated or interrupt therapy. A mathematical model and its parameters could be used to diagnose the assumption of superinfection of hepatocytes and to understand the causes for the spontaneous fluctuation pattern of HBV DNA loads...

journal_title：Intervirology

authors： Wang K,Tan W,Tang Y,Deng G

更新日期：2011-01-01 00:00:00

abstract：:Hepatitis B virus (HBV) core antigen (HBcAg) detected by the peroxidase-antiperoxidase technique was present in the nucleus and cytoplasm of some infected hepatocytes but only in the cytoplasm of other hepatocytes. When cells expressing HBcAg were examined by in situ hybridization for the presence of HBV DNA, the intr...

journal_title：Intervirology

authors： Gowans EJ,Burrell CJ,Jilbert AR,Marmion BP

更新日期：1985-01-01 00:00:00

abstract：OBJECTIVES:To explore the host transcription factor E2F-1 and/or any mechanisms that may support viral entry and its effect on the HSV-1 production in anti-CD3-activated Jurkat cells. METHODS:The expressions of ICP4, HVEM and E2F-1 were studied using reverse transcription-PCR and Western blot. HSV-1 production was det...

journal_title：Intervirology

authors： Bhattarakosol P,Donchai P

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:Human respiratory syncytial virus (RSV) infection in infancy and early childhood causes acute bronchiolitis and exacerbates bronchial asthma. Eosinophil infiltration may contribute to airway obstruction in RSV infection. OBJECTIVE:We hypothesized that RSV affects eosinophil function. METHODS:Eosinophil act...

journal_title：Intervirology

authors： Tachibana A,Kimura H,Kato M,Nako Y,Kozawa K,Morikawa A

更新日期：2002-01-01 00:00:00

abstract：:Serological and physical properties of isolated particles as well as reactions of barley cultivars after virus inoculation showed that the yellow disease of barley is caused by different viruses. Results summarized in this paper suggest that the European isolates BaYMV-So and BaYMV-NM are closely related to, or identi...

journal_title：Intervirology

authors： Huth W,Adams MJ

更新日期：1990-01-01 00:00:00

abstract：:There are two major requirements for potyvirus taxonomy; group-specific criteria and criteria which discriminate between distinct viruses and strains. This review discusses the relative merits in potyvirus taxonomy of molecular parameters, such as gene sequence data, nucleic acid hybridization, coat protein sequence d...

journal_title：Intervirology

authors： Ward CW,Shukla DD

更新日期：1991-01-01 00:00:00

abstract：OBJECTIVE:To investigate the effect of HIV Tat protein on Bcl-2 expression in human monocytes, and observe apoptosis of Tat-stimulated monocytes induced by TNF-alpha-related apoptosis-induced ligand (TRAIL). METHODS:Western blot was used to detect Bcl-2 expression in monocytes stimulated by HIV Tat protein, and Annexi...

journal_title：Intervirology

authors： Zheng L,Yang Y,Guocai L,Pauza CD,Salvato MS

更新日期：2007-01-01 00:00:00

abstract：:Cytomegalovirus infection of Brown Norway rats was studied after intraperitoneal or subcutaneous inoculation of virus. No clinical illness was apparent during the 1st month postinfection (p.i.). Low titers of virus were detected in many organs at day 4 p.i. for the intraperitoneally inoculated animals and at day 11 p....

journal_title：Intervirology

authors： Bruggeman CA,Meijer H,Bosman F,van Boven CP

更新日期：1985-01-01 00:00:00

abstract：OBJECTIVE:The aim of this study is to investigate the molecular mechanism of hepatocellular carcinoma (HCC) development induced by hepatitis B virus X protein (HBx). METHODS:We previously established a H7402-X cell line that constitutively expresses HBx protein. In the present study, H7402-X gene expression profiles a...

journal_title：Intervirology

authors： Ye L,Dong N,Wang Q,Xu Z,Cai N,Wang H,Zhang X

更新日期：2008-01-01 00:00:00

abstract：:Human cytomegalovirus (CMV) has devised numerous means of escaping immune surveillance. The CMV genome encodes at least 4 genes involved in downregulating surface expression of HLA class I molecules. In addition, it sequesters CC chemokines, induces Fc receptors, interferes with induction of HLA class II antigens, and...

journal_title：Intervirology

authors： Michelson S

更新日期：1999-01-01 00:00:00

abstract：:We examined influenza virus strains of the subtype H3N2 from outbreaks of respiratory diseases in swine herds in Germany. Four different clusters can be distinguished when comparing parts of the HA1 gene from porcine H3N2 isolates analyzed between 1982 and 2001. Comparison between these clusters reveals a bp homology ...

journal_title：Intervirology

authors： Schrader C,Süss J

更新日期：2004-01-01 00:00:00

abstract：:An amyloid-like isoform of a 33- to 34-kD glycoprotein, termed as the scrapie prion protein (PrPsc), plays a critical role in transmissible spongiform encephalopathies of animals and humans. It has even been suggested to present the responsible infectious agent. This protein is a posttranslationally modified form of t...

journal_title：Intervirology

authors： Tanji K,Saeki K,Matsumoto Y,Takeda M,Hirasawa K,Doi K,Matsumoto Y,Onodera T

更新日期：1995-01-01 00:00:00

abstract：AIMS:The objective of this work was to study the distribution of hepatitis C virus (HCV) genotypes and subtypes from 2010 to 2015 in 1,221 anti-HCV/HCV-RNA-positive specimens from patients living in the metropolitan area of Naples, since HCV genotypes and subtypes remain cornerstones in the management of chronic HCV in...

journal_title：Intervirology

authors： Grimaldi E,Della Pepa ME,Martora F,Magliocca P,Iovene MR,Coppola N,Donnarumma G,Galdiero M

更新日期：2017-01-01 00:00:00

abstract：:The complete sequence of the single-stranded, positive-sense RNA genome of pea stem necrosis virus (PSNV) has been determined. The 4,048-nucleotide genome contains five open reading frames (ORFs). The 5'-proximal ORF encodes a 25-kD protein (p25). If the amber termination codon is read through, the ORF produces a read...

journal_title：Intervirology

authors： Suzuki S,Hase S,Takahashi H,Ikegami M

更新日期：2002-01-01 00:00:00

abstract：:The PLC/PRF/5 human hepatoma cell line producing hepatitis B surface antigen (HBsAg) was studied to determine whether infectious hepatitis B virus (HBV) was also being produced. 2 chimpanzees with no previous exposure to HBV and no serologic markers of past or active HBV infection were inoculated intravenously with 50...

journal_title：Intervirology

authors： Tabor E,Copeland JA,Mann GF,Howard CR,Skelly J,Snoy P,Zuckerman AJ,Gerety RJ

更新日期：1981-01-01 00:00:00

abstract：:The Holland strain of anatid herpesvirus (AHV) was 2-to 10-fold more efficiently plaqued under liquid medium or semisolid medium containing methylcellulose than in media containing agar, agarose, or Nobel agar. Virus adsorption was complete in 45 min, with the maximum virus titers obtained under liquid medium at 48 h ...

journal_title：Intervirology

authors： Attanasio R,Olson R,Johnson JC

更新日期：1980-01-01 00:00:00

abstract：:The intracellular fate of 3H-labeled exogenous herpes simplex virus type 2 DNA following the exposure of recipient cells to selected DNA facilitators and the ultrastructural changes which ensued in transfected cultures were examined. In most cases, electron microscopic radioautography demonstrated that exogenous DNA r...

journal_title：Intervirology

authors： Marciano-Cabral F,Cabral GA,Taylor MM,Patterson M,Farber FE

更新日期：1981-01-01 00:00:00

abstract：:The envelope glycoprotein of the mouse mammary tumor virus (MMTV gp52), a useful plasma marker for mammary tumors in mice, was measured in extracellular medium by radioimmunoassay to quantitatively compare gp52 levels released by a C3H MMTV producer and its subcloned B9 nonproducer cell line. Experiments analyzed leve...

journal_title：Intervirology

authors： Ritzi EM,Walthall EB

更新日期：1989-01-01 00:00:00

abstract：:An open-reading frame designated 1R1, on DNA component 1 of bean golden mosaic geminivirus (BGMV), has been identified as the coat protein gene. A DraI restriction fragment of BGMV DNA1 that includes 1R1 was inserted into the SmaI pKK223-3 expression vector. The 32 kD protein expressed in Escherichia coli cells reacte...

journal_title：Intervirology

authors： Murayama A,Yamaya Y,Ikegami M

更新日期：1991-01-01 00:00:00

abstract：OBJECTIVE:In this study we established a mouse brain injection system to detect infectious dengue-2 virus produced from the full-length RNA transcripts. METHODS:In vitro transcription was used to synthesize full-length dengue-2 virus RNA from the plasmid pRS424FLDEN2NGC, which was intracerebrally injected into the 6-d...

journal_title：Intervirology

authors： Lee YR,Huang KJ,Lei HY,Chen SH,Lin YS,Yeh TM,Liu HS

更新日期：2005-01-01 00:00:00

abstract：OBJECTIVE:Characterization of the phylogeny and diversity of human respiratory syncytial virus (HRSV) genotype ON1 that occurred during its early evolution (within the first 3.5 years since the detection of the first ON1 strains). ON1 strains have a 72-nucleotide-long in-frame duplication within the second hypervariabl...

journal_title：Intervirology

authors： Ivancic-Jelecki J,Forcic D,Mlinaric-Galinovic G,Tesovic G,Nikic Hecer A

更新日期：2015-01-01 00:00:00

abstract：:The ability to discriminate between herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) infections by serological means is of increasing importance for clinical virological diagnostics. HSV type-specific serology has a number of applications, e.g. to guide duration and dosage of antiviral therapy, to allow for string...

journal_title：Intervirology

authors： Bergström T,Trybala E

更新日期：1996-01-01 00:00:00

abstract：OBJECTIVE:Hepatitis C virus (HCV) infection is a public health problem and a major cause of chronic hepatitis. This virus exhibits a great genetic variability, with 8 genotypes and numerous subtypes. The aim of this study was to evaluate the fluctuations of HCV subtypes during 2 decades in Venezuela. METHODS:HCV genot...

journal_title：Intervirology

authors： Loureiro CL,Jaspe RC,Pujol FH

更新日期：2019-01-01 00:00:00

abstract：:Transcription of human T-cell leukemia virus type I is regulated by a viral transactivatior Tax, through the 21-bp sequence in the long terminal repeat (LTR). We found that cellular transcription factor AP-1 (c-Jun/c-Fos heterocomplex) bound to the 21-bp sequence. The binding affinity of the complex increased in propo...

journal_title：Intervirology

authors： Fujii M,Tsuchiya H,Meng XB,Seiki M

更新日期：1995-01-01 00:00:00

abstract：:Damage and necrosis of hepatocytes in viral hepatitis C is considered to be immune mediated as in hepatitis B. Hepatocellular necrosis accompanies infiltration of lymphocytes and this feature, called necroinflammation, characterizes all types of viral hepatitis and corresponds to the histological expression of hepatit...

journal_title：Intervirology

authors： Uchida T

更新日期：1994-01-01 00:00:00

abstract：:Early hepatocellular carcinoma (HCC) as defined by the Liver Cancer Study Group of Japan would correspond to high-grade dysplastic nodules with small foci of HCC in the majority of cases, using the classification system proposed by the International Working Party. A large number of early HCCs were revealed to be hypo-...

journal_title：Intervirology

authors： Takayasu K,Muramatsu Y,Mizuguchi Y,Moriyama N,Ojima H

更新日期：2004-01-01 00:00:00