Abstract:
:In this study, we analyzed the in vitro expression, potency and longevity of immune responses induced in a Balb/c mouse model by a synthetic HIV-1 GAG gene exhibiting a codon usage that was adapted to that of highly expressed mammalian genes (syngag). In contrast to a vector containing the wild-type (wt) GAG gene, the syngag construct enabled highly efficient Gag expression in both human and rodent cell lines in complete absence of Rev and Rev-responsive element. Immunization of Balb/c mice with the wt gag plasmid DNA induced only weak and inconsistent humoral immune responses. Mice vaccinated by syngag but not wt gag developed substantial and highly consistent Gag-specific antibody titers showing a clear T helper 1 polarization even with low doses of DNA. Moreover, vaccinated mice developed a strong Gag-specific cellular immune response, including cytotoxic T cells, which was not observed in wt gag-immunized animals. Both humoral and cellular immunity were efficient and lasted for more than 20 weeks. Furthermore, the induction of the humoral as well as the cellular immune response was independent of the immunization route (intramuscular or subcutaneous). These results clearly show the advantages of codon-optimized genes with respect to the expression and immunogenicity of plasmid DNA constructs, making them promising vaccine candidates for further studies.
journal_name
Intervirologyjournal_title
Intervirologyauthors
Bojak A,Wild J,Deml L,Wagner Rdoi
10.1159/000067919keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
275-86issue
4-6eissn
0300-5526issn
1423-0100pii
67919journal_volume
45pub_type
杂志文章相关文献
INTERVIROLOGY文献大全abstract::Methods and models for analytical and quantitative researches on the evolution of hepatitis viruses using nucleotide sequences and amino acids of hepatitis viruses are described. Methods for the calculation of evolutionary rates and the construction of molecular evolutionary trees, especially by estimating the number ...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000024975
更新日期:1999-01-01 00:00:00
abstract::Two DNA-negative temperature-sensitive mutants of human cytomegalovirus strain AD169 were shown to be deficient in induction of several prominent viral polypeptides when grown in human foreskin fibroblasts under nonpermissive conditions. The use of a monospecific polyclonal antiserum allowed recognition of a further d...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150034
更新日期:1988-01-01 00:00:00
abstract::Cultures of human cells transformed by SV40 were found to release infectious virus, even after several passages in vitro. Virus shedding by these cultures did not depend on propagation of virus from cell to cell, as it was not affected by anti-SV40 antiserum that could effectively block virus propagation in acutely in...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000148919
更新日期:1978-01-01 00:00:00
abstract:BACKGROUND/AIMS:The present study was launched to systematically characterize the molecular genotypes and quantitative serological biomarkers during the natural course of chronic hepatitis B (CHB) infection in a patient population from Chongqing, China. METHODS:A total of 140 patients were enrolled and grouped accordi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000323524
更新日期:2012-01-01 00:00:00
abstract::The genetic relationship between two enteroviruses causing the acute hemorrhagic conjunctivitis (AHC) syndrome was examined by the RNA-RNA hybridization technique. Nucleotide sequence homology between the J670/71 strain of enterovirus type 70 (EV70) and the EH 24/70 strain of Singapore epidemic conjunctivitis (SEC) vi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149539
更新日期:1984-01-01 00:00:00
abstract::Hepatitis C virus (HCV) induces chronic liver disease in hosts which can eventually progresses to liver cirrhosis and hepatocellular carcinoma. However, progression of liver disease is slower in patients with persistently normal levels of alanine aminotransferase (ALT) than in those with active hepatitis. Although dis...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000087264
更新日期:2006-01-01 00:00:00
abstract::Echinochloa ragged stunt virus (ERSV) and rice ragged stunt virus (RRSV; Reoviridae) were purified from their respective host plants and disrupted in SDS. Evidence for the double-strandedness of ERSV RNA was obtained. Electrophoresis in 10% polyacrylamide gels resolved the RNAs of each virus into 10 segments, ranging ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150103
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND:Dengue virus is spread in tropical areas of the world and is the causative agent of dengue fever and dengue hemorrhagic fever. It is horizontally transmitted to humans by infected Aedes mosquitoes, but it is also able to be vertically or transovarially transmitted to insect progeny. OBJECTIVE:In this work, ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000107272
更新日期:2007-01-01 00:00:00
abstract::The Holland strain of anatid herpesvirus (AHV) was 2-to 10-fold more efficiently plaqued under liquid medium or semisolid medium containing methylcellulose than in media containing agar, agarose, or Nobel agar. Virus adsorption was complete in 45 min, with the maximum virus titers obtained under liquid medium at 48 h ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149193
更新日期:1980-01-01 00:00:00
abstract::In this study, 574 stool samples from children with gastroenteritis were obtained from different hospitals in Costa Rica, Honduras, Nicaragua and the Dominican Republic during 2005-2006. Diarrhea stool samples were analyzed for rotavirus by ELISA and typed by the RT-PCR-based method. Unusual strains were detected: G1P...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000317288
更新日期:2010-01-01 00:00:00
abstract::Dimethyl sulfoxide and twelve related polar compounds, capable of inducing differentiation of murine erythroleukemia cells, markedly inhibited induction of Epstein-Barr virus antigens in virus producer and nonproducer lymphoblastoid cells exposed to 12-O-tetradecanoylphorbol-13-acetate, n-butyric acid, 5-iodo-2'-deoxy...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150088
更新日期:1989-01-01 00:00:00
abstract::Human skin fibroblasts have previously been reported to display an age-dependent resistance to infection with coxsackie B4 virus. We have shown that the virus will replicate and produce CPE in human skin fibroblasts regardless of the age of the donor of the cells. The passage history of the virus was found to influenc...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150029
更新日期:1988-01-01 00:00:00
abstract::Human papillomavirus (HPV) DNA was isolated from a clinically diagnosed flat wart and proved to be related to HPV2. The isolate showed 55% cross-hybridization with HPV2a. A physical map of restriction enzyme cleavage sites differed completely from those of HPV2a and HPV2b. The new HPV2 subtype, which will be classifie...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149548
更新日期:1984-01-01 00:00:00
abstract:OBJECTIVES:The aims of this study were to determine the hepatitis B virus (HBV) genotypes in hepatitis delta virus (HDV) RNA-positive patients and to characterize the HBV nucleotide sequences that may be found on a distant island of Japan. METHODS:This study included three patients with chronic hepatitis who were posi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000069746
更新日期:2003-01-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) latency was established in human embryo lung (HEL) cells by treatment with (E)-5-(2'-bromovinyl)-2'-deoxyuridine and human leukocyte interferon and subsequent temperature increase (from 37 degrees to 40.5 degrees). This in vitro system was used to study reactivation after temperatur...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149668
更新日期:1986-01-01 00:00:00
abstract::3 mM n-butyrate, which effectively induces synthesis of Epstein-Barr virus (EBV) antigens in the producer P3HR-1 cell line, inhibited the transformation of umbilical cord blood lymphocytes by EBV. The drug inhibited both EBNA synthesis and stimulation of host cell DNA synthesis by strain B95-8. This effect was less pr...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149566
更新日期:1985-01-01 00:00:00
abstract::Human skin fibroblasts were previously shown to be resistant to coxsackie B4 virus infection. We have cultured fibroblasts from skin and lung tissues of donors of various ages. By a novel method for direct assay of virus absorption and penetration, we have shown that skin fibroblasts from young fetuses are susceptible...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149360
更新日期:1983-01-01 00:00:00
abstract::In order to assess the risk of transmission of viral diseases during floods, the viral burden in flooded areas of the city of Dresden (Germany) in August 2002 was investigated. Water samples were collected from 9 sampling sites and tested for the presence of 11 enteric viral pathogens. As a control, water samples from...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000095157
更新日期:2006-01-01 00:00:00
abstract::By a combination of PCR and direct-cycle sequencing using consensus primers, we analyzed approximately 400-bp fragments within the NS3 genes of twenty-one dengue virus type 3 strains isolated from five neighboring Southeast Asian countries at different time intervals from 1956 to 1992. The majority of base disparities...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150386
更新日期:1994-01-01 00:00:00
abstract::The critical point dried (CPD) whole cell technique was applied to the study of the morphogenesis and morphology of rabies virus and vesicular stomatitis virus (VSV) in mouse neuroblastoma and baby hamster kidney (BHK) cells. With the stereoscopic technique, progeny viruses at the cell surface and within the cytoplasm...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000148939
更新日期:1978-01-01 00:00:00
abstract::Studies of human papillomavirus (HPV) infection are hampered by the lack of an adequate culture system. The athymic mouse xenograft system permits propagation of HPV, but only a few HPV types have been grown in this manner. To produce an oncogenic type for studies of HPV pathogenesis, a condylomata acuminata lesion fr...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000025033
更新日期:2000-01-01 00:00:00
abstract::Cancer of the cervix is the most common malignant tumor among women in Africa and, in particular, Senegal. Studies of the prevalence of human papilloma virus (HPV) infection in Africa have mainly focused on carcinomas. Data on the presence of the virus in women with normal cervical cytology are scarce. In this study, ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000024981
更新日期:1999-01-01 00:00:00
abstract::Genital infection with high-risk human papillomaviruses (HPV) has been etiologically linked with the development of cervical and other anogenital cancers. There is therefore a need for an effective HPV vaccine with the potential to significantly reduce the burden of more than half a million new cervical cancer cases i...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000150475
更新日期:1996-01-01 00:00:00
abstract::6/94 virus, isolated originally from a multiple sclerosis (MS) patient, was compared antigenically with related parainfluenza type 1 strains. These included two Sendai strains of mouse and two Sendai strains of reported human origin as well as the HA2 strain. By standard hemagglutination inhibition (HI) or hemadsorpti...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149875
更新日期:1975-01-01 00:00:00
abstract::Transcription of human T-cell leukemia virus type I is regulated by a viral transactivatior Tax, through the 21-bp sequence in the long terminal repeat (LTR). We found that cellular transcription factor AP-1 (c-Jun/c-Fos heterocomplex) bound to the 21-bp sequence. The binding affinity of the complex increased in propo...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150436
更新日期:1995-01-01 00:00:00
abstract:OBJECTIVE:To investigate the effect of HIV Tat protein on Bcl-2 expression in human monocytes, and observe apoptosis of Tat-stimulated monocytes induced by TNF-alpha-related apoptosis-induced ligand (TRAIL). METHODS:Western blot was used to detect Bcl-2 expression in monocytes stimulated by HIV Tat protein, and Annexi...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000100565
更新日期:2007-01-01 00:00:00
abstract::We have studied the effect of gamma-interferon (IFN-gamma) treatment on the transcription of viral and c-myc oncogenes in bovine papillomavirus type 1 (BPV-1)-transformed mouse fibroblast cell lines. Upon IFN-gamma treatment, viral transcripts always decreased in cell lines containing episomal BPV-1 DNA, while the eff...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150320
更新日期:1993-01-01 00:00:00
abstract:OBJECTIVE:Cytomegalovirus (CMV) is widely distributed and constitutes the main cause of congenital infections worldwide. CMV transmission during pregnancy represents one of the major impacts of this virus on public health. This study aimed at assessing glycoprotein B (gB) CMV genotypes in Mexican children and pregnant ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000373922
更新日期:2015-01-01 00:00:00
abstract::A serologic comparison was performed by immune electron microscopy between hepatitis A antigen (related to the MS-1 strain of hepatitis A virus) and a viral isolate, CR69(076), from the stool of a patient in Costa Rica with viral hepatitis. CR69(076) was not antigenically related to hepatitis A antigen but was related...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149486
更新日期:1975-01-01 00:00:00
abstract::We examined the effect of infection by other herpesviruses on the induction of Epstein-Barr virus (EBV) antigens. Raji cells were infected with a variety of viruses before superinfection with P3HR-1 virus 6 h later. Much earlier and greater synthesis of EBV antigens was observed in herpesvirus (herpes simplex virus ty...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150057
更新日期:1988-01-01 00:00:00