Abstract:
AIMS:The objective of this work was to study the distribution of hepatitis C virus (HCV) genotypes and subtypes from 2010 to 2015 in 1,221 anti-HCV/HCV-RNA-positive specimens from patients living in the metropolitan area of Naples, since HCV genotypes and subtypes remain cornerstones in the management of chronic HCV infection even in the directly acting antivirals era. METHODS:The study was carried out on 1,221 anti-HCV/HCV-RNA-positive plasma samples collected between April 2010 and December 2015. RESULTS:Of the 1,221 patients enrolled, 633 (51.9%) were males and 588 (48.1%) were females, with a mean age of 60 ± 13 (SD) years. The most frequent HCV genotype observed was genotype 1 (68.1%; 1b in 55.3% and 1a in 9.5%); HCV genotype 2 was found in 289 samples (23.67%), genotype 3 in 6.47%, genotype 4 in only 19 samples, and only 2 samples were classified as genotype 5. The mean age of the patients with genotype 1a or 3 was lower (51 ± 12 and 49 ± 12 years, respectively) than those with genotype 1b (62 ± 11, p < 0.0001 for both) or 2 (62 ± 14, p < 0.0001 for both). CONCLUSIONS:The data from the present study suggest that HCV genotype 1b remains the most prevalent in this area, followed by genotype 2, 1a, and 3a.
journal_name
Intervirologyjournal_title
Intervirologyauthors
Grimaldi E,Della Pepa ME,Martora F,Magliocca P,Iovene MR,Coppola N,Donnarumma G,Galdiero Mdoi
10.1159/000481821subject
Has Abstractpub_date
2017-01-01 00:00:00pages
82-89issue
3eissn
0300-5526issn
1423-0100pii
000481821journal_volume
60pub_type
杂志文章相关文献
INTERVIROLOGY文献大全abstract::When Vero cells infected with Junín virus are cultured in the presence of 16 mM glucosamine, no infectious virus is produced and no surface antigens or virions are detected. Cytoplasmic viral antigen, however, is synthesized normally. ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149032
更新日期:1979-01-01 00:00:00
abstract::We examined the effect of infection by other herpesviruses on the induction of Epstein-Barr virus (EBV) antigens. Raji cells were infected with a variety of viruses before superinfection with P3HR-1 virus 6 h later. Much earlier and greater synthesis of EBV antigens was observed in herpesvirus (herpes simplex virus ty...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150057
更新日期:1988-01-01 00:00:00
abstract::In chicken cells infected by Sindbis virus and exposed to a variety of membrane-active compounds, virus release was inhibited. In infected cells exposed to antiserum directed against the virion glycoproteins E1 or E2, retinol, cortisone, Pb++ or insulin, the processing of two Sindbis virus precursor polypeptides which...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149063
更新日期:1979-01-01 00:00:00
abstract::In less than 1% of persistently infected individuals, human T cell lymphotropic virus type I (HTLV-I) causes a chronic inflammatory disease of the central nervous system (CNS), called HTLV-I-associated myelopathy/tropical spastic paraparesis. Important prerequisites for disease induction are oligoclonal expansion of H...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150540
更新日期:1997-01-01 00:00:00
abstract:OBJECTIVE(S):Chikungunya virus (CHIKV) is a reemerging virus of significant importance that has caused large-scale outbreaks in the countries with a temperate climate. CHIKV causes debilitating arthralgia which can persist for weeks and up to a year. Fibroblast cells are the main target of CHIKV infection. In this stud...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000441309
更新日期:2015-01-01 00:00:00
abstract::The regulatory regions for transcription and replication of several hepatitis B virus (HBV) genomes from 19 patients having various forms of HBV infection were sequenced. Predominant mutations were found to occur naturally in nucleotide positions 1762 (A to T) and 1764 (G to A) in chronic hepatitis patients and in asy...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150453
更新日期:1995-01-01 00:00:00
abstract::Mammalian hepatitis E virus (HEV), the etiological agent of hepatitis E in humans, is a recently discovered infectious agent. It was identified for the first time in 1983 using electron microscopy on a faecal specimen of a person infected with non-A, non-B enterically-transmitted hepatitis. Based on retrospective and ...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000342301
更新日期:2013-01-01 00:00:00
abstract::To evaluate the diagnostic value of nucleic-acid-sequence-based amplification (NASBA) for the detection of cytomegalovirus (CMV) infection in transplant recipients, we compared immediate early 1 (IE1) and late pp67 mRNA detection by NASBA with the antigenemia assay, PCR and viral culture in 72 renal transplant (RTx) r...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000053974
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:Although human leukocyte antigens (HLA) have been shown in association with the outcomes of hepatitis C virus (HCV) infection among different ethnic groups, such studies remain absent in China, where the HCV prevalence is higher than the global average. METHODS:In this study, 426 HCV-infected and 709 uninfe...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000369209
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:Syncytia formation is the hallmark of the cytopathic effect caused by human respiratory syncytial virus (HRSV), which is the most important viral respiratory pathogen in children. This article reports methodological improvements in primary HRSV isolation and the importance of syncytia formation and mRNA level...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000480014
更新日期:2017-01-01 00:00:00
abstract::Methods and models for analytical and quantitative researches on the evolution of hepatitis viruses using nucleotide sequences and amino acids of hepatitis viruses are described. Methods for the calculation of evolutionary rates and the construction of molecular evolutionary trees, especially by estimating the number ...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000024975
更新日期:1999-01-01 00:00:00
abstract::Examination of thin sections from the cerebral cortex of scrapie-infected hamster brains revealed characteristic circular 26-30 nm diameter tubulofilamentous particles, identical to those previously described in both experimentally induced scrapie in mice, hamsters and natural scrapie of sheep, bovine spongiform encep...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150269
更新日期:1992-01-01 00:00:00
abstract::The ability to discriminate between herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) infections by serological means is of increasing importance for clinical virological diagnostics. HSV type-specific serology has a number of applications, e.g. to guide duration and dosage of antiviral therapy, to allow for string...
journal_title:Intervirology
pub_type: 杂志文章,评审
doi:10.1159/000150493
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND/AIMS:Cardiovirus is a genus of viruses belonging to the family Picornaviridae. Here, we used viral metagenomic techniques to detect the viral nucleic acid in the fecal samples from wild rats in Zhenjiang city in China. METHOD:Fecal samples were collected from 20 wild rats and pooled into four sample pools a...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000500555
更新日期:2019-01-01 00:00:00
abstract::Rotavirus is the most common cause of severe diarrhoea worldwide, affecting over 125 million young children every year in developing countries. The present study is a part of ongoing childhood diarrhoeal surveillance to determine the strain diversity of rotaviruses prevalent in Andaman and Nicobar Islands. From Octobe...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000342219
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:Human Salivirus (SalV) has been associated with gastroenteritis on all continents. METHODS:This paper presents the real-time RT-PCR assay for the detection of SalV in clinical fecal samples collected from 192 hospitalized children with acute gastroenteritis in Piedmont, Italy. RESULTS:The most commonly dete...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000490568
更新日期:2018-01-01 00:00:00
abstract::Echinochloa ragged stunt virus (ERSV) and rice ragged stunt virus (RRSV; Reoviridae) were purified from their respective host plants and disrupted in SDS. Evidence for the double-strandedness of ERSV RNA was obtained. Electrophoresis in 10% polyacrylamide gels resolved the RNAs of each virus into 10 segments, ranging ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150103
更新日期:1989-01-01 00:00:00
abstract::The morphology, assembly, and accumulation sites of rhabdovirus particles in Peregrinus maidis planthoppers infected with a Hawaiian isolate of maize mosaic virus (MMV) were studied. These particles were usually bullet-shaped, but were sometimes bacilliform, and averaged 234 and 247 nm, respectively, in length and 60 ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149616
更新日期:1985-01-01 00:00:00
abstract::Scrapie brain homogenate was mixed with mouse peritoneal macrophages in vitro. After 2 h of incubation at 37 degrees, a portion of the scrapie infectivity was associated with macrophages. In contrast, very little infectivity was associated with kidney cells that had been exposed to scrapie brain homogenate. After incu...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149241
更新日期:1981-01-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) latency was established in human embryo lung (HEL) cells by treatment with (E)-5-(2'-bromovinyl)-2'-deoxyuridine and human leukocyte interferon and subsequent temperature increase (from 37 degrees to 40.5 degrees). This in vitro system was used to study reactivation after temperatur...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149668
更新日期:1986-01-01 00:00:00
abstract::Studies of human papillomavirus (HPV) infection are hampered by the lack of an adequate culture system. The athymic mouse xenograft system permits propagation of HPV, but only a few HPV types have been grown in this manner. To produce an oncogenic type for studies of HPV pathogenesis, a condylomata acuminata lesion fr...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000025033
更新日期:2000-01-01 00:00:00
abstract::Three independently isolated herpes simplex virus type 1 recombinant viruses containing a deletion of approximately 14 kilobase pairs, representing greater than 95% of the internal repeat DNA sequences, were analyzed for their pathogenicity in mice. The recombinant viruses were found to be avirulent, exhibiting drasti...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000150147
更新日期:1990-01-01 00:00:00
abstract:OBJECTIVES:Chikungunya virus (CHIKV) is an alphavirus belonging to the Togaviridae family. Alphaviruses cause a chronic non-cytopathic infection in mosquito cells, while they develop a highly cytopathic infection in cells originating from various vertebrates. In this study, we compared the cytopathic effect (CPE) induc...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000339985
更新日期:2013-01-01 00:00:00
abstract::A BHK/21 cell line persistently infected by an arenavirus is described. During four consecutive passages, 30-45% of the cells showed granular cytoplasmic antigen by indirect immunofluorescence, employing both Argentine hemorrhagic fever convalescent sera and sera from animals immunized with Junin virus. Virus isolated...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149180
更新日期:1980-01-01 00:00:00
abstract:OBJECTIVES:To characterize morphological, physicochemical and genomic features of a novel virulent coliphage which was isolated from an engineered Escherichia coli culture and termed engineered E. coli phage (EEP). METHODS AND RESULTS:Electron microscopy revealed that EEP has an icosahedral head (62 nm in diameter) an...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000299063
更新日期:2010-01-01 00:00:00
abstract::In this study, 574 stool samples from children with gastroenteritis were obtained from different hospitals in Costa Rica, Honduras, Nicaragua and the Dominican Republic during 2005-2006. Diarrhea stool samples were analyzed for rotavirus by ELISA and typed by the RT-PCR-based method. Unusual strains were detected: G1P...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000317288
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVES:Single-nucleotide polymorphisms (SNPs) near the interleukin (IL) 28B gene encoding a type III interferon (IFN-λ) are the most important genetic predictors of treatment response to hepatitis C virus (HCV). This retrospective study was undertaken to determine any association between IL28B SNPs and the developm...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000357326
更新日期:2014-01-01 00:00:00
abstract::An RNA virus was isolated from Trichoplusia ni larvae infected with inoculum of the baculovirus of Autographa californica. Physicochemical characterization has revealed a 35-nm virus with a density in CsCl of 1.3 g/cm3 and a sedimentation coefficient of 200S. The virus consisted of 10--15% single-stranded RNA with an ...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149040
更新日期:1979-01-01 00:00:00
abstract::Hepatitis B virus (HBV) core antigen (HBcAg) detected by the peroxidase-antiperoxidase technique was present in the nucleus and cytoplasm of some infected hepatocytes but only in the cytoplasm of other hepatocytes. When cells expressing HBcAg were examined by in situ hybridization for the presence of HBV DNA, the intr...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000149646
更新日期:1985-01-01 00:00:00
abstract::Ferret hepatitis E virus (HEV), a novel hepatitis E-like virus, has been identified in ferrets in the Netherlands, Japan, and the US. To determine whether ferret HEV transmits to other animals, we inoculated laboratory rats (Wistar), nude rats (Long-Evans-rnu/rnu), and cynomolgus monkeys with ferret HEV (F4351) by int...
journal_title:Intervirology
pub_type: 杂志文章
doi:10.1159/000373891
更新日期:2015-01-01 00:00:00