Abstract:
AIMS:The aim of the present study is the preliminary characterization of an El Tor vibriophage M4 (ATCC 51352-B4). METHODS:We studied the growth characteristics and sustainability of this phage under various stresses like temperature, pH, and UV. The phage morphology and phage genome were also examined using electron microscopy. RESULTS:Sustainability studies showed that the phage is more stable in acidic conditions, which is very uncommon among vibriophages. Studies also showed that M4 is a thermostable phage and it is inactivated by temperatures above 60°C but, like other phages, UV has a high inactivating effect on it. Morphological and genomic studies by electron microscopy showed that this phage has a long contractile tail and a big head. The genome is linear and about 120 kb in length. The genome also has a high packaging density as the value of Vm (the volume occupied per Da of biological macromolecule) is low for this phage. The phage-bacterial interaction was studied by negative staining as well as ultrathin sectioning methods. CONCLUSIONS:M4 belongs to the Myoviridae family and it is generally thermostable. It is prone to destruction by alkali and UV. It also has a large DNA which is densely packed inside of a big capsid.
journal_name
Intervirologyjournal_title
Intervirologyauthors
Das S,Ghosh ANdoi
10.1159/000485835subject
Has Abstractpub_date
2017-01-01 00:00:00pages
149-155issue
4eissn
0300-5526issn
1423-0100pii
000485835journal_volume
60pub_type
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