A new family of vertebrate viruses: Toroviridae.

Abstract:

:The proposed family Toroviridae is characterized by eveloped, peplomer-bearing particles containing an elongated tubular nucleocapsid with helical symmetry. The capsid may bend into an open torus, conferring a biconcave disk or kidney-shaped morphology to the virion (largest diameter 120-140 nm) or the capsid may be straight, resulting in a rod-shaped particle (35 X 170 nm). Morphogenesis is by budding of preformed nucleocapsids through membranes mainly of the Golgi system and of the rough endoplasmic reticulum. Berne virus, which is proposed as the family prototype, contains a single strand of infectious positive-sense RNA, Mr about 6.5 X 10(6), which is polyadenylated. The RNA is surrounded by the major nucleocapsid phosphoprotein (Mr about 20,000) which, in turn, is enveloped by a membrane containing one major protein (Mr 22,000) and a phosphoprotein (Mr 37,000). The viral peplomers, about 20 nm long, carry determinants for neutralization and hemagglutination; they are formed by a polydisperse N-glycosylated protein (Mr 75,000-100,000). Four major subgenomic polyadenylated RNAs have been identified in infected cells, with Mrs of 3.0, 0.71, 0.46 and 0.26 X 10(6). Torovirus replication is inhibited by actinomycin D, alpha-amanitin and pre-irradiation of the host cell with UV light. All toroviruses identified so far cause enteric infections and are probably transmitted by the fecal-oral route. Serologic relationships between equine, bovine and human toroviruses have been demonstrated.

journal_name

Intervirology

journal_title

Intervirology

authors

Horzinek MC,Flewett TH,Saif LJ,Spaan WJ,Weiss M,Woode GN

doi

10.1159/000149710

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

17-24

issue

1

eissn

0300-5526

issn

1423-0100

journal_volume

27

pub_type

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